Postharvest Storage Research Articles

Antifungal activity of Cinnamomum camphora essential oil against Fusarium oxysporum and its application on postharvest blueberry fruits preservation

SummaryPostharvest decay of blueberry fruits caused by Fusarium oxysporum (F. oxysporum) results in significant losses during logistics. To avoid chemical residue issues associated with synthetic fungicides, present study investigated the antifungal activity of Cinnamomum camphora essential oil (CEO) against Fusarium oxysporum (F. oxysporum) in postharvest blueberry fruits. CEO effectively disrupted cell membrane integrity, impaired ergosterol structure, restricted ATPase activity and inhibits mycelial growth. Blueberries treated with a 0.2% CEO‐based coating demonstrated improved overall sensory acceptability, enhanced firmness, increased total soluble solids and titratable acidity, and reduced decay rate. CEO also decreased malondialdehyde contents while promoting ascorbic acid and glutathione levels and activating antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), maintaining cell membrane structure and enhancing resistance against F. oxysporum infection. The findings suggest that CEO serves as an eco‐friendly agent for mitigating primary fungal invasion in blueberries during postharvest storage.

Volatile flavor behavior characterization of Hericium erinaceus during postharvest storage using E-nose, HS-GC-IMS, and HS-SPME-GC–MS after treated with electron-beam generated X-ray irradiation

Flavor alteration is a crucial factor affecting the quality of mushrooms during preservation. The dynamic variations of volatile profiles of fresh Hericium erinaceus with electron-beam generated X-ray irradiation were investigated by combining E-nose, headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS), and headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC–MS). E-nose analysis achieved rapid discrimination in all treatments over storage time. 65 and 73 volatile organic compounds (VOCs) were identified by HS-GC-IMS and HS-SPME-GC–MS, respectively. Thereinto, 1-octen-3-ol, 1-octen-3-one, and 2-octanone were screened out as the characteristic VOCs, which contents declined during storage. While the contents of (E)-2-octenal, (E)-2-nonenal, and 1-octanol increased. The flavor profile changes from distinct mushroom and floral odor to an intense alcohol and fatty odor. Notably, one-kGy irradiation remained more volatiles and denser mushroom odor after storage. Multivariate analysis further confirmed that 1.0 kGy irradiation contributed to the overall aroma retention during postharvest storage of H. erinaceus.

Application of Carboxymethyl Cellulose and Its Edible Composite Coating in Fruit Preservation

ABSTRACTFruits are highly susceptible to quality deterioration and spoilage during postharvest storage. Edible coating emerges as an effective strategy to prolong the postharvest life of fruit and enhance their quality during storage. Carboxymethyl cellulose (CMC) is a polysaccharide derivative widely applied in various industries due to its excellent barrier and mechanical properties. CMC has been utilized as an edible coating material for postharvest preservation of fruit for many years. Ongoing research is centred on the development of composite films by integrating CMC with natural biopolymers or potential active agents to augment their functionality in postharvest fruit preservation. This review consolidates findings on composite coatings of CMC combined with various materials for fruit storage and preservation, shedding light on the challenges associated with such composite coatings.

Delayed Luminescence in Monitoring the Postharvest Ripening of Tomato Fruit and Classifying According to Their Maturity Stage at Harvest

Ripening is an irreversible phenomenon involving several physiological, biochemical and organoleptic changes that affect the nutritional value, colour, texture and taste of fruit. Amongst the physical non-destructive technologies, Delayed Luminescence (DL) has been reported able to provide valid information on the functional status of living biological systems, reflecting changes in quality parameters following the occurrence of certain metabolic processes. In this study, we followed up and analysed the variations in the colour and DL signals emitted by tomato fruit as a function of their ripening degree. Four lots of tomato fruit, similar to each other for cultivar and geographical origin, were considered. Tomatoes were harvested at various maturity stages and stored in the dark at room temperature. The changes in DL emission trends were monitored at different times after harvesting for a period of about 20 days and correlated with the a*/b* colour ratio, which is normally used as an indicator of fruit ripeness degree. Results highlighted that the emission intensities, as well as the parameters by fitting DL decay curves, were strongly dependent on harvest maturity, varying significantly even as fruit ripeness progressed after their harvesting. Significant differences were also found between DL signals emitted by field-ripened tomatoes at the beginning of storage and those emitted by postharvest-ripened tomatoes at the end of the storage period. Delayed Luminescence may be used as a fast, reliable and sensitive tool for evaluating, more generally, the sensorial quality of fruit during postharvest storage.

Enhancing post-harvest quality of tomato fruits with chitosan oligosaccharide-zinc oxide nanocomposites: A study on biocompatibility, quality improvement, and carotenoid enhancement

In this study, a new composite with combination of chitosan oligosaccharide (COS) and zinc oxide nanoparticles (ZnO NPs), termed Chitosan Oligosaccharide-Zinc Oxide Nanocomposites (COS-ZnO NC), was designed to enhance the quality of tomato fruits during postharvest storage. SEM analysis showed a uniform distribution of COS-ZnO NC films on tomato surfaces, indicating high biocompatibility, while the FTIR spectrum confirmed the interaction of COS and ZnO NPs via hydrogen bonds. The COS-ZnO NC exerts positive effects on post-harvest quality of tomato fruits, including significantly reduced water loss, fewer skin wrinkles, increased sugar-acid ratio, and enhanced vitamin C and carotenoids accumulation. Furthermore, COS-ZnO NC induces transcription of carotenoid biosynthesis genes and promotes carotenoids storage in the chromoplast. These results suggest that the COS-ZnO NC film can significantly improve the quality traits of tomato fruits, and therefore is potential in post-harvest storage of tomato fruits.

Improved prediction of vitamin C and reducing sugar content in sweetpotatoes using hyperspectral imaging and LARS-enhanced LASSO variable selection

This study utilized least angle regression (LARS) with the least absolute shrinkage and selection operator (LASSO) to select important wavelengths for rapid quantification of vitamin C (Vc) and reducing sugar (RS) in sweetpotato roots (SPR) using hyperspectral imaging (900–1700 nm). Nine wavelengths strongly correlated with Vc levels and twelve with RS were identified, achieving good predictions with partial least squares (PLS) regression (Vc: rP = 0.9704, RMSEP = 1.0098 mg/100 g; RS: rP = 0.9641, RMSEP = 0.2725 g/100 g). Validation with an independent sample set (n = 35) showed minimal deviation between predicted and actual values (Vc: 1.179–1.211 mg/100 g; RS: 0.316–0.324 g/100 g). Explainable AI and SHapley Additive exPlanations (SHAP) values were used to interpret the selected wavelengths. Chemical maps visually analyzed Vc and RS distribution in different SPR samples. This method effectively estimates Vc and RS levels in SPR, potentially aiding SPR quality assessment during post-harvest marketing and storage.

The role of hydrogen-rich water in delaying the pulp breakdown of litchi fruit during postharvest storage

Previous studies have indicated that hydrogen-rich water (HW) treatment can delay fruit ripening and senescence. However, little is known about the HW-delaying pulp breakdown. In this study, eight physiological characteristics revealed that HW treatment delayed both pericarp browning and pulp breakdown of litchi fruit. To gain a comprehensive understanding of the changes in litchi pulp, a combination of multiple metabolomics and gene expression analyses was conducted, assessing 67 primary metabolites, 103 volatiles, 31 amino acids, and 13 crucial metabolite-related genes. Results showed that HW treatment promoted starch degradation, decelerated cell wall degradation and glycolysis, and maintained the flavor and quality of litchi fruit. Furthermore, HW treatment stimulated the production of volatile alcohols, aldehydes, ketones, olefins, and amino acids, which might play a vital role in HW-delaying pulp breakdown. This study sheds light on the mechanism by which HW delayed pulp breakdown by investigating small molecule metabolites and metabolic pathways.

Preharvest Elicitors as a Tool to Enhance Bioactive Compounds and Quality of Both Peel and Pulp of Yellow Pitahaya (Selenicereus megalanthus Haw.) at Harvest and during Postharvest Storage.

Yellow pitahaya is a tropical fruit that has gained popularity in recent years. Natural elicitors are compounds that can stimulate the resistance and quality of fruits. The objective of this study was to evaluate the effects of natural elicitors, methyl salicylate (MeSa), methyl jasmonate (JaMe), salicylic acid (SA) and oxalic acid (OA) at concentrations of 0.1 mM (MeSa and JaMe) and 5 mM (SA and OA), applied to the yellow pitahaya fruits under greenhouse conditions. After full blossom, four applications were made with a frequency of 15 days. At the time of harvest and after storage, the following variables were evaluated: firmness (whole fruit), total soluble solids (TSS), total acidity (TA), phenolics and carotenoids (in the pulp), while phenolics, carotenoids, macronutrients and micronutrients were determined in the peel. The results showed MeSa advanced the fruit maturation, according to higher TSS, lower TA and firmness than MeJa-treated fruits, for which a delayed ripening process was shown. All treatments induced a higher polyphenolic concentration during storage. Regarding the alternative use of the peel as a by-product, the application of natural elicitors significantly increased the content of polyphenols, carotenoids, macronutrients and micronutrients in the peel, especially MeSa, which can be used as a bioactive compound in the food industry. In conclusion, the results indicate that natural elicitors can be an alternative to improve the quality and shelf life of yellow pitahaya fruits.

Strawberry Post-Harvest Anthocyanin Development to Improve the Colour Stability of Strawberry Nectars

Strawberry nectars have increased colour stability when produced from overripe, darker, and redder strawberries, with a high anthocyanin concentration. The post-harvest storage of strawberries has been shown to develop these properties. Nectars are frequently produced from strawberries rejected for fresh sale due to poor colour, which are insufficiently ripe to produce colour-stable nectars. This study investigated post-harvest anthocyanin development in strawberries to improve the colour and colour stability of nectars, which is the first time these developments were studied for beverage production. Strawberries at five ripeness stages were stored at 20 °C for 1 and 2 days prior to nectar production. The anthocyanin content of nectars was determined by a pH-differential method, and the colour stability was tracked for 12 weeks using a consumer Acceptance Factor, derived from CIELAB colour components. The anthocyanin content and colour stability were highly correlated, and both were dependent on ripening, with larger increases observed in under-ripe strawberries, and small to no improvement in overripe samples. Stored partially coloured strawberries produced nectars with equivalent colour stability to non-stored strawberries of normal ripeness. This allowed strawberries that were previously unsuitable for both fresh sale and nectar production to be used as a feedstock for nectar production, reducing food waste.

Botanical extracts improve postharvest quality and extend the shelf life of papaya (Carica papaya L. cv. Shahi)

ABSTRACT Though papaya is a lucrative fruit regarding nutritional, medicinal, and commercial attributes, its susceptibility to postharvest loss may account for ≥ 40%. An experiment was carried out at Khulna University, Bangladesh from April to May 2013 to investigate the effects of botanical extracts on the postharvest physicochemical attributes, intensity, and severity of diseases and shelf life of papaya cv. Shahi. The experiment comprised botanical extracts from onion bulb, neem leaf, garlic bulb, oriental pepper leaf, datura leaf, and control as six treatments and replicated thrice following a completely randomised design (CRD). Among the physicochemical parameters, total soluble solids (TSS), weight loss, and dry matter of papaya fruit increase, and pulp to peel ratio, moisture content decrease gradually during the storage period disregarding treatments. Garlic bulb extract decreases the incidence and severity of postharvest diseases and extends the shelf life by more than 7 days compared to control. Thus, garlic bulb extract can be used for postharvest papaya storage. However, further experiments should be conducted before the final recommendation.

Effect of chitosan-tripolyphosphate to suppress anthracnose (Colletotrichum spp.) in post-harvest chili

Anthracnose disease (Colletotrichum spp.) in chili can lead to low fruit quantity and quality from planting to the postharvest stage. The environmentally friendly management using chitosan (CS) is expected to suppress anthracnose disease and increase the shelf-life of chili. This study aimed to determine the effect of the chitosan-tripolyphosphate (CS-TPP) to suppress anthracnose on chili during postharvest storage through in-vitro and in-vivo assays. In the in-vitro assay, CS-TPP solution with a ratio of [5:2] and [3:1] was applied into a warm Potato Dextrose Agar (PDA) medium. It was grown by Colletotrichum pathogen, while on in vivo assay, CS-TPP was applied to chili fruit before being inoculated by the pathogen. A control treatment was prepared without CS-TPP application. Results revealed that the CS-TPP ratios affected the growth of Colletotrichum spp. at the in-vitro assay. The CS-TPP [5:2] ratio was more effective than CS-TPP [3:1] in reducing the growth of Colletotrichum spp. with the fungal inhibition of 62,65% and 55,56%, respectively, compared to the control treatment. Moreover, it also showed anthracnose disease suppression on chili fruit of 51%, and 29%, respectively, compared to control treatment at in-vivo assay. This study showed the potential use of CS-TPP as a coating application for anthracnose disease management on storage chili, however further study such as viability and longevity of formula need to be done.

A dual-branch selective attention capsule network for classifying kiwifruit soft rot with hyperspectral images

Kiwifruit soft rot is highly contagious and causes serious economic loss. Therefore, early detection and elimination of soft rot are important for postharvest treatment and storage of kiwifruit. This study aims to accurately detect kiwifruit soft rot based on hyperspectral images by using a deep learning approach for image classification. A dual-branch selective attention capsule network (DBSACaps) was proposed to improve the classification accuracy. The network uses two branches to separately extract the spectral and spatial features so as to reduce their mutual interference, followed by fusion of the two features through the attention mechanism. Capsule network was used instead of convolutional neural networks to extract the features and complete the classification. Compared with existing methods, the proposed method exhibited the best classification performance on the kiwifruit soft rot dataset, with an overall accuracy of 97.08% and a 97.83% accuracy for soft rot. Our results confirm that potential soft rot of kiwifruit can be detected using hyperspectral images, which may contribute to the construction of smart agriculture.

Postharvest storage temperature strategies affect anthocyanin levels, total phenolic content, antioxidant activity, chemical attributes of juice, and physical qualities of blood orange fruit

The potential of storing ‘Budd Blood’ blood orange fruit at different temperatures (6, 8, 10, and 12 °C) for 60 days after harvest was investigated to enhance anthocyanin, total phenolic content, antioxidant activity, and to understand temperature effects on other physicochemical attributes. Fruit weight and firmness losses increased during storage, with the lowest losses occurring at 6 °C. Titratable acidity (TA) decreased, and the lowest values were recorded at 10 and 12 °C. Total soluble solids (TSS), TSS/TA, and juice pH increased, with the highest values recorded at 10 and 12 °C. The sucrose content exhibited slight changes while glucose and fructose levels increased during storage, with the highest levels of glucose and fructose observed at 12 °C. Total anthocyanin concentration, the levels of cyanidin-3-glucoside and cyanidin 3-(6″-malonylglucoside), total phenolic content, and total antioxidant activity significantly increased during storage and the highest values were observed at 10 and 12 °C. Total anthocyanin content increased 2950 % at 10 °C and 3000 % at 12 °C. Interestingly, higher temperatures (10 and 12 °C) were more effective in enhancing anthocyanin levels, total phenolic content, and antioxidant activity during storage, while lower temperatures (6 and 8 °C) were more effective in preserving the other physicochemical attributes.

Detection of Fusarium spp. infection in potato (Solanum tuberosum L.) during postharvest storage through visible–near-infrared and shortwave–near-infrared reflectance spectroscopy

Abstract Pathogen infection can damage agricultural products, thereby reducing their economic value. Fusarium spp. is a fungal pathogen that infects potatoes (Solanum tuberosum L.) and causes dry rot. In this study, we utilized visible–near-infrared (Vis–NIR) and shortwave–near-infrared (SW–NIR) spectroscopy for the early detection of Fusarium spp. infection in potato tubers. The spectrometer used in this study analyzed the Vis–NIR (400–1,000 nm) and SW–NIR (970–1,700 nm) regions. A total of 183 potato (G2 “Granola L.” variety) samples were used. Among these, 93 samples were artificially inoculated with Fusarium solani mycelia, while 90 samples were left uninfected and considered the control group. The potato samples were stored at two different temperatures (12 and 25°C). Vis–NIR and SW–NIR spectra were analyzed by a chemometric method, namely principal component analysis with linear discriminant analysis (PCA–LDA), to differentiate healthy and infected potatoes. The PCA–LDA model based on Vis–NIR spectra exhibited a calibration accuracy of 80.26% and a reliability of 65%. Meanwhile, the PCA–LDA model based on SW–NIR spectra exhibited a calibration accuracy of 100% and a reliability of 97.30%. Overall, both methods demonstrated their suitability for differentiating potato tubers with Fusarium spp. fungal infection and healthy ones; however, the results suggest that SW–NIR spectroscopy is more effective than Vis–NIR spectroscopy.

Postharvest Treatment Effects on ‘Somerset Seedless’ Cold-Hardy Table Grapes

ABSTRACT Limited amount of information is available for cold-hardy table grape postharvest storage and strategies to extend the storage time. ‘Somerset Seedless’ is a cold-hardy table grape with a potential market for the Upper Midwest and Northern Great Plains. Postharvest treatments were assessed as a possible route to increase cold-hardy table grape shelf-life. In this study, a 1.4% chitosan postharvest treatment was tested on ‘Somerset Seedless’ grapes through 7 weeks of storage with temperature 1–4°C, humidity ≥90%. The effects were compared to two controls: one was diH2O, and the other one was acetic acid. The assessment included grape appearance traits (rachis, decay, mold, scattering, and splitting), physiochemical properties (TSS, pH and total acidity), antioxidant activity, as well as total phenolics and flavonoid content. In general, 1.4% chitosan, 1% acetic acid, and diH2O had a large impact on grape appearance during storage with limited impact on chemistry. Throughout 7 weeks of storage, no significant differences were observed in grape physiochemical and phytochemical changes. Chitosan performed the best for ‘Somerset Seedless’ postharvest storage in regard to the low rate of visible damage. Although acetic acid had similarly positive effects on mold control as chitosan treatment, it caused the highest split rate after 1 week and the highest shatter rate after 5 weeks of storage. Chitosan treated ‘Somerset Seedless’ still met the USDA standard of table grapes after 5 weeks. This study suggests chitosan postharvest treatments may have applications for enhancing the shelf-life of cold-hardy table grapes.

Untargeted metabolomic analysis of strawberries exposed to pulsed electric fields and cold plasma before postharvest storage

After being harvested, strawberries experience a decline in nutrients and anthocyanins, which is further exacerbated by their vulnerability to plant pathogen-related decay. As postharvest losses encompass up to 50 % of total production, the development of a physical method complementary to refrigeration to reduce these losses is a goal pursued globally, given a global market of US$19 B per year. In this study, two non-thermal technologies, pulsed electric fields (PEF) and cold plasma (CP), were used to evaluate their effectiveness in maintaining phytochemical integrity in exposed strawberries. A PEF treatment of 1 pulse at 1 kV/cm field strength in 3 L of tap water could significantly alter the volatile and metabolomic composition of the fruit, while simultaneously reducing its firmness during cold storage. However, subjecting the fruit to a CP treatment at 15 % (210 watts) for 1 min did not impact the quality parameters. Furthermore, unlike the PEF treatment, the strawberries retained their firmness during storage and exhibited a consistent volatile and metabolomic profile. Based on these results, CP treatment enhances firmness and maintains the compounds found in strawberries, meanwhile, while PEF treatment might not be ideal for preserving the physicochemical parameters of fruit

Application of plasma-activated hydrogen peroxide solution synergized with Ag@SiO2 modified polyvinyl alcohol coating for strawberry preservation

To extend the postharvest storage time of strawberries, this study aims to prepare a composite coating using plasma-activated hydrogen peroxide solution (PAH) synergized with nano-Ag@SiO2 by blending method to modify polyvinyl alcohol (PVA) solution. Results showed that the viscosity and the swelling rate of PVA significantly decreased with the addition of Ag@SiO2 at 0.18 %. Meanwhile, the elongation at break and the tensile strength of PVA increased to 0.87 MPa and 214 %. When the addition of Ag@SiO2 was 0.18 % and the composite ratio of PAH to PVA was 1:1, the composites could inactivate the pathogenic bacteria at 2 h. During the storage of strawberries, the initial colony counts on the surface of strawberries could be reduced by about 1 lg CFU/g after coating with the composite film. Moreover, with the extension of the storage time to 7 d, the respiratory intensity, colony counts, and rot index in the strawberries were 65.7 mg/(kg•h), 4.05 log CFU/g, and 38.7 %. Meanwhile, the superoxide dismutase activity and Vc content were 944 U/g and 690 μg/g, respectively. Overall, this study provides ideas and the theoretical basis for applying composite films in fruit preservation.

Preservation of Postharvest Quality of Fresh-Cut Cauliflower through Exogenous Putrescine, Citric Acid and Salicylic Acid Treatments

Cauliflower is among the perishable vegetables after harvest. In recent years, the popularity of fresh-cut cauliflower has increased among consumers as a minimally processed product. This study was carried out to determine the effects of exogenous putrescine (PUT), citric acid (CA) and salicylic acid (SA) treatments on postharvest quality of fresh-cut cauliflower stored at 4±0.5 °C and 90±5% relative humidity for 21 days. In the study, a total of seven different treatments using two different doses (0.5 and 1.0 mM) of PUT, CA and SA were investigated. To evaluate the quality of fresh-cut cauliflower, the necessary measurements and analyzes were performed at periodic intervals on days 0, 7, 14, and 21 of storage. The results demonstrated that quality properties changed significantly depending on the postharvest treatments and storage durations. With the increase of storage duration, weight loss and ash content increased, while K, Zn and Cu contents decreased. In general, the treatments examined in the study had positive effects on postharvest quality of cauliflower. Among the treatments, especially 1.0 mM PUT was found to be more effective. Compared to the control, 1.0 mM PUT treatment decreased weight loss by 61.32%, though it increased protein content by 14.48% and P content by 21.55%. It was concluded that 1.0 mM PUT treatment can be recommended as an alternative application method to extend the storage life of fresh-cut cauliflower and reduce postharvest quality losses.

Examining starch metabolism in lotus roots (Nelumbo nucifera Gaertn.) during post-harvest storage at different temperatures

This study explores the impact of postharvest storage temperatures (4 °C and 25 °C) on starch metabolism and textural attributes of glutinous lotus root. While starch metabolism is a well-known factor influencing texture, changes in powdery and sticky qualities have remained unexplored. Our research reveals that storing lotus roots at 4 °C delays water dissipation, amylopectin reduction, and the decline in textural elements such as hardness, adhesiveness, springiness, gumminess, and resilience. Lower temperatures postpone amylopectin reduction and sugar interconversion, thereby preserving the sticky texture. Additionally, they suppress starch formation, delay starch metabolism, and elevate the expression of genes involved in starch metabolism. The correlation between gene expression and root texture indicates the critical role of gene regulation in enzyme activity during storage. Overall, low-temperature storage extends lotus root preservation by regulating metabolite content, enzyme activities, and the corresponding genes involved in starch metabolism, preserving both intrinsic and external root quality.

Simulated logistical transport effects on textural change of wax apple using different packaging types during storage time

The textural damage caused by logistics transport is a major problem during the post-harvest logistics and storage time of agricultural products. To address the issue, this study investigated the impact on the textural change of wax apple with different packaging types by conducting international safe transit association 6-amazon. com-sioc type A (ISTA 6 A) tests and measured the changes in related indicators over the eight-day storage time period at 15 ℃. The results demonstrated that compared to non-vibrated wax apple of the same packaging type, the damage index of the vibration-treated wax apple increased to 0.39 at the end of storage time. This led to more noticeable damage to fruit texture in the vibration-treated wax apple. With the same outer packaging type, different types of expanded polyethylene (EPE) cushioning materials presented varying degrees of textural damage following vibration treatment. The order was double-layer foam net (DL-FN, 0.23) < single-layer foam net (SL-FN, 0.38) < EPE liner (EPE-L, 0.50). The superior cushioning performance of DL-FN could distribute vibratory energy across the entire wax apple to reduce partial textural injury, but SL-FN provided weaker cushioning protection. Furthermore, it was more beneficial for the dissipation of respiratory heat to suppress the related cell-wall enzymes activities and prevented internal cottony softening of wax apple by maintaining an appropriate environment within the packaging, while EPE-L prevented respiratory heat dissipation and water diffusion. Using the same cushioning type, no significant difference was observed in the damage of fruit texture between the wax apple packaging by foam box (FB, 0.25) and corrugated box (CB, 0.23). The finding suggested that when the outer package had sufficient supporting performance, the cushioning type required not only adequate cushioning property, but also excellent vapor and gas permeability in order to facilitate respiratory heat diffusion and better protect the textural integrity of the wax apple in the process of logistics. This protection was crucial for the development of wax apple in e-commerce logistics.