The objectives of this investigation were to 1) determine serum concentrations of progesterone (P 4), estrone sulfate (E 1S) and pregnancy-specific protein B (PSPB) from estrus synchronization through mid-gestation in the fallow doe ( Dama dama) and 2) characterize the hormonal profiles of does whose embryos or fetuses died in utero. Ten fallow does were synchronized for 14 d with an intravaginal P 4- releasing device (CIDR) and were naturally mated after CIDR removal. Blood samples were collected at CIDR insertion, CTDR removal and at intervals through Day 203 post-CIDR removal for analysis of P 4, E 1S and PSPB by radioimmunoassay (RIA). Ultrasonography was performed on Days 49 and 69 post-CIDR removal. Serum P 4 at the time of CIDR insertion was 4.8 ± 0.6 ng/ml, and at CIDR withdrawal it was 6.2 ± 0.3 ng/ml. Concentrations of E 1S and PSPB were nondetectable at CIDR insertion. Serum E 1S was highest at Day 93, and PSPB was first detectable in pregnant does at Days 27 to 30 post-CIDR withdrawal. Ultrasonography on Day 49 revealed that 6 does were pregnant, 2 were not pregnant and 2 others were diagnosed originally as early pregnant. At Day 69, ultrasonography revealed that 6 does (60%) were pregnant and 4 (40%) were not. A comparison of the ultrasonographic and hormonal data indicated that the 2 does diagnosed as early pregnant on Day 49 had conceived but had lost the pregnancy. A third doe which was pregnant on Day 69 lost the fetus later in gestation. Hormonal profiles of does whose embryo or fetus had died were characterized by erratic P 4 and E 1S profiles, with PSPB becoming undetectable in the 3 does by Days 49, 65 and 80 post-CIDR removal. These data 1) demonstrate the timing for the collection of serum samples for determining early pregnancy in fallow does using 3 hormonal methods and 2) characterize the hormonal profiles of 3 fallow does with embryonic-fetal loss.