Prestressed stayed columns are vulnerable to fire due to material weakening. The fire behaviour of the stayed columns is investigated with non-uniform temperature distributions considered using steady state analysis within ABAQUS. This study aims to reveal the load-carrying capacity reduction of stayed columns in fire and propose a design method. Results show that ultimate loads decrease with temperature increasing, and the stayed columns approximate failure at the temperatures higher than 600°C. The failure deflected mode maintains similar to the dominant linear buckling mode for the uniform temperature distribution, and the sensitivity of ultimate loads to prestressing levels becomes less significant at higher temperatures. Moreover, compared to buckling and ultimate behaviour, initial post-buckling behaviour shows more significant sensitivity to temperature distributions. The non-uniform distribution can lead to asymmetric deflected mode due to stiffness re-distribution from the material weakening at elevated temperatures, and the post-buckling behaviour of the cases with rotational symmetric mode dominance is sensitive to prestressing levels. Finally, a fire design method is proposed based on the ultimate load reduction in fire.