Positive Rate Of anti-HCV Research Articles

Comparison of clinical value of two kinds of imunoassay method in the detection of related antibody of patients with HCV

Objective To compare the clinical value of two kinds of imunoassay method including CLIA and ELISA in the detection of related antibody of patients with HCV. Methods 60 patients with HCV were chosen as study subjects, and the related antibodies of patients were detected by CLIA and ELISA.The positive rates of anti-HCV antibody, anti-3E antibody, anti-GP210 antibody, anti-SP100 antibody, anti-PML antibody and anti-AMA-M2 antibody detected by CLIA and ELISA were compared. Results The positive rates of anti-HCV antibody detected by CLIA and ELISA were 83.33%(50/60), 98.33%(59/60), respectively.The anti-HCV antibody detected by CLIA was significantly higher than by ELISA(χ2=4.73, P=0.03). The positive rates of anti-3E antibody, anti-GP210 antibody, anti-SP100 antibody, anti-PML antibody and anti-AMA-M2 antibody detected by ELISA were 11.67%(7/60), 6.67%(4/60), 11.67%(7/60), 10.00%(6/60), 11.67%(7/60), respectively, those detected by CLIA were 73.33%(44/60), 50.00%(30/60), 38.33%(23/60), 58.33%(35/60), 80.00%(48/60), respectively.The positive rates of anti-3E antibody, anti-GP210 antibody, anti-SP100 antibody, anti-PML antibody and anti-AMA-M2 antibody detected by CLIA were significantly higher than by ELISA(χ2=18.81, 11.51, 13.23, 15.08, 13.75, all P<0.05). Conclusion Compared with ELISA, CLIA in the detection of related antibodies of patients with HCV can efficiently higher the positive rate of anti-HCV and autoantibody. Key words: Chemiluminescent measurements; Enzyme-linked immunosorbent assay; Hepatitis C antibodies

Rate of infection and related risk factors on hepatitis C virus in three counties of Jiangsu province

To investigate the hepatitis C virus (HCV) infection rate among general population in Jiangsu province and to analyze the main risk factors for HCV infection. Subjects in 3 counties were surveyed by stratified cluster random sampling in the National Major Science and Technology Projects demonstration area in Jiangsu province. Interview was carried out with uniformly- designed questionnaires. Blood samples were collected and anti-HCV tested. Data were analysed under SPSS 13.0. Case-control study was conducted on two groups with subjects under 1:4 matching by sex, age, village of residence and status of HCV infection. Cox's proportional hazards regression analysis was then performed to analyze the risk factors for HCV infection. The positive rate of anti-HCV was higher in females (0.965%, 95%CI:0.899%-1.035%) than in males (0.572%, 95%CI:0.517%-0.632%). Significant differences were noticed among the positive rates of anti-HCV in the three counties (0.131%, 95%CI:0.103%-0.164%;0.316%, 95%CI: 0.268%-0.370%; 2.173% , 95% CI:2.039%-2.315% , respectively). Peak prevalence of anti-HCV (1.577%, 95%CI:1.425%-1.740%) was observed among persons at 50-59 years of age. Bottom rate (0.161%, 95%CI:0.125%-0.204%) was observed below 30 years of age. High anti-HCV positive rate was detected in people with lower education background or belonged to 'floating population'. Factors as having histories of hospitalization (OR = 3.049, 95% CI:1.322-7.036), blood transfusion (OR = 14.319, 95%CI:2.318-88.459) or sharing razor (OR = 3.604, 95%CI:1.096-11.851) were risk factors of HCV infection in the area with the lowest anti-HCV positive rate. In another county, factor as having histories of 'blood products transfusion' (OR = 7.202, 95% CI:1.170-44.310) appeared important while in the third county, having history of 'blood donation' (OR = 7.496, 95%CI:6.121- 9.180); 'blood transfusion' (OR = 2.305, 95%CI:1.578-3.369) and 'invasive physical examination' (OR = 1.258, 95% CI:1.021-1.549) appeared to be important. Age seemed a risk factor for HCV infection. "Sharing razor" was a specifically important risk factor among the 30- year-olds. "Having received acupuncture" was noticed a risk factor only among people at 50-59 years of age while "Having received invasive physical examination" was important in the 50-59 and 60-69 year-olds. High anti-HCV positive rate was observed in people at middle of aged population, as well as in those with poor education or under 'floating'. Unsafe blood donation or having received blood-product transfusion might be the risk factors in the last decades. Factors as sharing razor, having received acupuncture or invasive examination might be specifically risky in different populations.

Analysis on hepatitis C virus infection and related factors among newly reported HIV infections in Henan province

To study hepatitis C virus infection and related factors among newly reported HIV infections in Henan province. Both HIV-1 BED incidence and anti-HCV tests were applied on newly reported HIV-1 infections between 1 Jul. 2012 to 30 Jun. 2013 in Henan province. 2049 HIV-1 infections were newly reported between 1 July 2012 to 30 June 2013 in Henan province, with the positive rate of anti-HCV as 14.87% (271/1 887) and the proportion of BED positives was 26.34% (497/1 887). The recent HIV-1 infections appeared lower HCV prevalence than non-recent HIV-1 infection. Among 20-39 age group, HIV-1 infections had a lower HCV prevalence than among the other age groups. Patients carrying HIV-1 through injecting drug use showed higher HCV prevalence. Under results on BED; age, infection route and area were influencing factors of HCV prevalence in newly reported HIV-1 infections in Henan province, suggesting that corresponding prevention programs should be targeted.

Hepatitis C viruses infection situation in the human population of six provinces in China

To investigate the seroprevalence of hepatitis C viruse infection in the human population in six regions of Beijing, Heilongjiang, Shandong, Ningxia, Gansu and Sichuan in China. ELISA was used for detecting anti-HCV IgG of the serum samples. All sample were collected in 2006-2008 in six areas. 9538 samples were detected. The total positive rate of anti-HCV was 0.39% (37), 0.23% (3/1328) in Beijing, 0.74% (12/1629) in Heilongjiang, 0.26% (5/1962) in Shandong, 0.1% (1/1000) in Ningxia, 0.44% in Gansu (9/2 037) and Sichuan (7/1582), respectively. The 37 positive samples at the sex were 19 (51.35%) of man and 18 (48.65%) of female. At the age group were 1 (2.70%) of < 10 years old, 5 (13.51%) of 10-19 years old, 4 (10.81%) of 20-29 years old, 6 (16.22% ) of 30-39 years old, 9 (24.32%) of 4049 years old, 12 (32.43%) of > or = 50 years old. The positive samples were detected anti-HAV-IgG, anti-HEV-IgG and HBsAg/ HBcAb /HBeAb. The positive Number was 35 (94.59%), 10 (27.03%) and 2 (5.41%) respectively. HCV infection rate in the population was 0.1% -0.74%, 1/3 of > or = 50 years old in HCV positive. Hepatitis C virus co-infection with HAV, HEV and HBV.

Variation in the host ABO blood group may be associated with susceptibility to hepatitis C virus infection

This study aimed to determine the relationship between hepatitis C virus (HCV) infection and ABO/Rhesus blood groups, age and sex. A total of 20 000 patients who came to donate blood in the blood bank of GSVM Medical College, Kanpur were enrolled in the study. Demographic data recorded for each patient included age, sex and blood group. Blood samples were tested for anti-HCV antibodies and ABO/Rhesus blood group antigen typing was performed. The overall positive rate of anti-HCV was 0.34%. We found that seropositivity for HCV increased with age. Anti-HCV antibodies were detected in 1/765 women (0.13%), compared to 67/19 235 men (0.35%). Seroprevalence of HCV was found to be higher in blood group O individuals (0.42%) and lowest in blood group AB individuals (0.04%). The results of this study demonstrate that that HCV infection may not be related to age and sex but the possible association of blood group antigens with HCV infection cannot be ruled out.