You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research II1 Apr 20121007 IMMEDIATE AND DELAYED INTRACAVERNOUS (IC) INJECTION OF ADIPOSE-DERIVED STROMAL VASCULAR FRACTION (SVF) ENHANCE RECOVERY OF ERECTILE FUNCTION IN CAVERNOUS NERVE (CN) INJURED RATS Xuefeng Qiu, Thomas M. Fandel, Ludovic Ferretti, Maarten Albersen, Haiyang Zhang, Hazem Orabi, Guiting Lin, Ching-Shwun Lin, Tania Schroeder, and Tom F. Lue Xuefeng QiuXuefeng Qiu San Francisco, CA More articles by this author , Thomas M. FandelThomas M. Fandel San Francisco, CA More articles by this author , Ludovic FerrettiLudovic Ferretti San Francisco, CA More articles by this author , Maarten AlbersenMaarten Albersen San Francisco, CA More articles by this author , Haiyang ZhangHaiyang Zhang San Francisco, CA More articles by this author , Hazem OrabiHazem Orabi San Francisco, CA More articles by this author , Guiting LinGuiting Lin San Francisco, CA More articles by this author , Ching-Shwun LinChing-Shwun Lin San Francisco, CA More articles by this author , Tania SchroederTania Schroeder Minnetonka, MN More articles by this author , and Tom F. LueTom F. Lue San Francisco, CA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1109AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES IC injection of cultured adipose tissue-derived stem cells (ADSC) effectively restores erectile function in CN injured rats. However, culturing exposes ADSC to risks of contamination and dedifferentiation. We compared the effect of uncultured SVF on improving erectile function when administered at the time of injury or four weeks after CN injury. METHODS SVF were procured from paratesticular fat in 89 male rats. Sixty-three rats were randomized into three groups: 1) IC injection of saline directly after CN crush (n=23), 2) IC injection of freshly isolated SVF directly after CN crush (n=17), 3) IC injection of freshly isolated SVF four weeks after CN crush (n=23). Twenty-six rats served as sham controls (group 4). Twelve weeks after injury, all rats underwent functional assessment of intracavernous pressure (ICP) during electrical stimulation of the CN. Penile tissues were harvested for immunohistochemistry and trichrome staining. The nNOS content within dorsal nerves and smooth muscle content within corpus cavernosum were assessed. One way ANOVA with post-testing was used to assess statistical significance. RESULTS Mean ICP/MAP ratio was 0.796 +/- 0.283, 0.999 +/- 0.175, 0.964 +/- 0.261, and 1.11 +/- 0.173 for groups 1, 2, 3, and 4, respectively. ICP/MAP ratio was significantly greater in sham rats relative to injured controls (p<0.05) and in acutely treated and delayed treated rats relative to injured controls (p<0.05). There was no significant difference in ICP/MAP ratio between acutely and delayed treated rats. The areas (%) of nNOS positive nerve fibers were 0.77 +/- 0.24, 1.19 +/- 0.25, 1.18 +/- 0.25, and 1.46 +/- 0.39 for groups 1, 2, 3, and 4, respectively. The ratios between smooth muscle (SM) and collagen (%) within corpus cavernosum were 4.5 +/- 1.8, 7.1 +/- 0.98, 7.0 +/- 1.7, and 8.9 +/- 2.2 for groups 1, 2, 3 and 4, respectively. The nNOS content within dorsal nerves and the ratio between SM and collagen were significantly greater in sham rats, acutely treated, and delayed treated rats relative to saline treated rats (p<0.05). There was no significant difference in nNOS positivity and fibrosis between acutely and delayed treated rats. CONCLUSIONS Freshly isolated autologous SVF injected after CN crush improved erectile function, promoted nerve regeneration and prevented fibrosis of the corpus cavernosum following CN injury. Comparable effects of SVF were observed when administrated acutely after injury and also in the chronic state 4 weeks after injury. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e409-e410 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Xuefeng Qiu San Francisco, CA More articles by this author Thomas M. Fandel San Francisco, CA More articles by this author Ludovic Ferretti San Francisco, CA More articles by this author Maarten Albersen San Francisco, CA More articles by this author Haiyang Zhang San Francisco, CA More articles by this author Hazem Orabi San Francisco, CA More articles by this author Guiting Lin San Francisco, CA More articles by this author Ching-Shwun Lin San Francisco, CA More articles by this author Tania Schroeder Minnetonka, MN More articles by this author Tom F. Lue San Francisco, CA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...