Information on sensitization of cattle to common allergens like Pyroglyphids or approved practical methods to evaluate in vivo sensitization are lacking. The aim of the study was to evaluate both intradermal testing (IDT) and sensitization to Dermatophagoides in healthy cattle and those infested with Psororptes or Chorioptes. Animals were included in three groups: Group A, healthy (n = 50), Group B, Psoroptes ovis infestation (n = 50), and Group C, Chorioptes bovis infestation (n = 12). Two methods of IDT were compared: classical use of 1 mL insulin syringe and a tuberculination device. Reactions to histamine 1/10,000 were compared to a negative control and commercial allergens (Dermatophagoides farinae, D. pteronyssinus) by injections (0.1 mL) in the midsection of the lateral neck. Readings were made at 30 min (diameter of the wheal and thickening of the skin fold with a calliper square) and at 48 h. The best results were obtained combining the tuberculin device and diameter of the wheal (100% correlation with the calliper square). Reactions to histamine were significantly more intense than for the allergens tested. Positive intradermal reactions included: Group A, D. farinae 42% and D. pteronyssinus 36%; Group B, D. farinae 74% and D. pteronyssinus 50%; Group C, D. farinae 58% and D. pteronyssinus 42%. No delayed reaction was detected. The possible use of this cross‐reactivity for a rapid evaluation of infestation of herds by parasitic mites needs more evaluation. Funding: Self‐funded.