Introduction: Vertical transmission by Toxoplasma gondii usually occurs in women who are infected for the first time during pregnancy. Screening for toxoplasmosis during pregnancy is one of the measures adopted in our country as a form of prevention. Objective: To investigate maternal risk factors associated with gestational toxoplasmosis in a large maternity hospital located in Fortaleza-Ceará. Methodology: This is an observational, quantitative comparative study conducted in a federal public maternity hospital located in Fortaleza, Ceará, Brazil in 2014. Data collection occurred from January to March 2014, through the analysis of medical records. guided by a data collection instrument. The research project was submitted to the Assis Chateaubriand School Maternity Research Ethics Committee (MEAC), and was approved in the register 513.392 / 2014-14. Results: The sample analyzed was established by high risk pregnant women aged 13 to 40 years. The diagnosis of toxoplasmosis was predominant in the age group between 20 and 30 years. As managers diagnosed with toxoplasmosis reside mainly in the interior of Ceará with statistically significant difference compared to pregnant women residing in the capital. Conclusion: It can be concluded that the analysis analyzed as the variables that were associated with toxoplasmosis were: residing within the state, gestational week between 13 to 27 weeks, normal sharing and using positive IgG serology for toxoplasmosis.