Event Abstract Back to Event Molecules, involved in purinergic signaling, are present on the surface of microglia-derived membrane vesicles Agnes Kittel1*, Petra Misják2, Dániel Boncz1, Erika Tischler1, Krisztina Palóczy2, Hardy Richter1, Beáta Sperlágh1 and Edit Buzás2 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary 2 Semmelweis Med School, Department Genetics, Cell- and Immunobiol, Hungary Membrane vesicles (MVs) are small (50-1000 nm) membrane-bound structures released by almost any kind of cell in physiological and pathophysiological conditions. They are considered as important mediators of intercellular communication, however, their role has not yet been revealed. Our goal was to investigate whether MVs can play a role in purinergic signaling. As a model system we used the BV2 microglia cell line. Microglia cells are very sensitive to changes in the concentration of extracellular ATP, and express NTPDase1 (an ectonucleotidase) and purinergic receptors. Among P2 receptors, P2Y12 was found on the cell process moving toward the higher ATP or nucleotide concentrations. We worked out a protocol for the preparation of MVs from tissue culture supernatants. Enzyme and immunocytochemistry were applied for demonstration of ecto-ATPase activity, expression of NTPDase1 and P2Y12 both in the cells and in their released MPs both in steady state condition and after LPS treatment. CD63 immunostaining was performed to identify exosomes, known to contain 5’–nucleotidase activity, an enzyme, which consumes AMP, the hydrolysation product of ecto-ATPDases. Our results show that even a microglial cell line is able to produce a wide range of MVs, different in size, morphology and in molecular composition. Furthermore, a challenge like LPS treatment can modify their composition and the ratio of MV subpopulations. Immonostaining revealed that mainly CD63 positive exosomes (50-100 nm in diameter) and irregular shaped microvesicles (100-1000nm) contain P2Y12 and NTPDase1, and the proportion of the latter one is increased after LPS treatment. These preliminary results underline the involvement of MVs in multiple biological processes and suggest their use as potential diagnostic tools. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Cellular neuroscience Citation: Kittel A, Misják P, Boncz D, Tischler E, Palóczy K, Richter H, Sperlágh B and Buzás E (2010). Molecules, involved in purinergic signaling, are present on the surface of microglia-derived membrane vesicles. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00074 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Apr 2010; Published Online: 21 Apr 2010. * Correspondence: Agnes Kittel, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, kittel@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Agnes Kittel Petra Misják Dániel Boncz Erika Tischler Krisztina Palóczy Hardy Richter Beáta Sperlágh Edit Buzás Google Agnes Kittel Petra Misják Dániel Boncz Erika Tischler Krisztina Palóczy Hardy Richter Beáta Sperlágh Edit Buzás Google Scholar Agnes Kittel Petra Misják Dániel Boncz Erika Tischler Krisztina Palóczy Hardy Richter Beáta Sperlágh Edit Buzás PubMed Agnes Kittel Petra Misják Dániel Boncz Erika Tischler Krisztina Palóczy Hardy Richter Beáta Sperlágh Edit Buzás Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.