Portion Of Wall Research Articles

Ising lines: Natural topological defects within ferroelectric Bloch walls

Phase-field simulations demonstrate that the polarization order-parameter field in the Ginzburg-Landau-Devonshire model of rhombohedral ferroelectric ${\mathrm{BaTiO}}_{3}$ allows for an interesting linear defect, stable under simple periodic boundary conditions. This linear defect, here called the Ising line, can be described as an about 2-nm-thick intrinsic paraelectric nanorod acting as a highly mobile borderline between finite portions of Bloch-like domain walls of opposite helicity. These Ising lines play the role of domain boundaries associated with the Ising-to-Bloch domain-wall phase transition.

Usefulness of a crista catheter for 3-dimensional electroanatomical mapping of complex right atrial tachyarrhythmias.

Right atrial (RA) tachyarrhythmias are not rare in patients with congenital heart disease and a history of cardiac surgery. This study investigated the usefulness of a crista catheter for 3-dimensional electroanatomical mapping of RA tachyarrhythmias. We consecutively included 35 patients (age, 43.2 ± 15.6 years; 15 men) who underwent an electrophysiological study with 3-dimensional electroanatomical mapping for RA tachycardia or flutter. In 13 patients with atrial flutter, we recorded and compared the electrical sequence in the anterior and posterior portions of the RA lateral wall. We used a crista catheter as a mapping catheter for 3-dimensional mapping in 12 patients (crista group), a lasso catheter in 12 patients (lasso group), and an ablation catheter in 11 patients (ablation group). We compared the 3-dimensional mapping points, time, and speed (mapping points per minute) among the groups. Atrial flutter was confirmed as cavotricuspid isthmus-dependent in all patients whose two atrial electrical sequences were the same direction and as atypical (including scar-related and dual-loop) in all patients whose sequences were in the opposite direction. Mapping speed in the crista group was significantly faster than in the lasso and ablation groups: median (interquartile range) 44.0 (35.5-69.4) points/min, 23.7 (17.8-29.8) points/min, and 8.2 (4.8-11.0) points/min, respectively (p = 0.001). A crista catheter is useful for high-density 3-dimensional electroanatomical mapping of complex RA tachyarrhythmias. Comparison of the electrical sequences in the anterior and posterior portions of the RA lateral wall is helpful for differentiating between cavotricuspid isthmus-dependent and atypical atrial flutter.

Contrast-enhanced ultrasonography diagnosis of fundal localized type of gallbladder adenomyomatosis.

BackgroundAdenomyomatosis of gallbladder is an acquired hyperplastic lesion, characterized by focal or diffuse thickening of the gallbladder with intramural cysts or echogenic areas with comet tail on ultrasonography. But in some cases, especially in the localized fundal type of adenomyomatosis, the intramural anechoic cystic spaces are uncertainty which causes difficult to differential adenomyomatosis from GB cancer. The purpose of this study was to determine the accuracy of real-time contrast-enhanced ultrasonography(CEUS) in the diagnosis of the fundal localized type of gallbladder adenomyomatosis.MethodsWe performed a retrospective study of 21 patients with pathologically proven fundal localized type of gallbladder (GB) adenomyomatosis. All patients underwent preoperative grayscale ultrasound (US) and real-time CEUS examination. The study’s reviewers made the diagnosis of adenomyomatosis according to the presence of the focal thickening of the fundal gallbladder wall with intramural cyst or intramural echogenic foci on grayscale US or CEUS. The diagnostic accuracy of US and CEUS was compared. The enhanced pattern and degree of intactness of the GB wall were also recorded.ResultsThe fundal portion of the GB wall showed localized thickness in all 21 patients. Small anechoic spaces or intramural echogenic foci were detected in 14 (66.7 %) and 21 (100 %) of cases respectively, and the intactness of the GB wall’s outer hyper-echoic layer was demonstrated in 17 (81 %) and 20 (95 %) on grayscale US and CEUS, respectively. The accuracy rate of the above two examination modalities was significantly different (p < 0.05). In the arterial phase of the CEUS, areas of focal thickened GB wall were iso-enhanced in 18 cases and hyper-enhanced in 3 cases. All 21 cases appeared to show heterogeneous enhancement with small non-enhancement spaces. The mucosal and serosal layers of the GB wall surrounding the lesions were enhanced, which presented as two “hyper-echoic lines” in the arterial phase of CEUS. In the venous phase of the CEUS, 19 lesions were iso-enhanced and 2 lesions were hypo-enhanced. The small non-enhancement spaces were more clearly during the venous phase.ConclusionThe small non-enhancement space is a characteristic finding of the fundal localized type of gallbladder adenomyomatosis on CEUS. CEUS could increase the degree of visualization of Rokitansky-Aschoff sinuses (RAS) and intactness of the GB wall, which play an important role in differential diagnosis.

EFFECT OF WALL THERMAL CONDUCTIVITY ON HYDROGEN-ASSISTED CATALYTIC IGNITION CHARACTERISTICS OF PROPANE-AIR AT MICRO-SCALES IN DIFFERENT FEEDING MODES

Effect of wall thermal conductivity on hydrogen self-ignition and hydrogen-assisted ignition of propane-air mixtures in different feeding modes from ambient cold-start conditions were investigated numerically with chemical kinetic model in Pt/γ-Al2O3 catalytic micro-combustors. For the steady and transient state, effect of wall thermal conductivity on self-ignition characteristics of lean hydrogen-air mixtures was presented, and hydrogenassisted combustion of propane-air mixtures was investigated numerically in the co-feed mode and the sequential feed mode. The computational results indicate the large thermal inertia of the micro-combustor solid structure leads to slow temperature dynamics, and transient response is dominated by the thermal inertia. The heat localization in poorly conducting walls leads to fast ignition and shorter steady state time. In general, the concentration of hydrogen required for propane ignition increased with increasing wall thermal conductivity, decreasing inlet velocity, and decreasing inlet equivalence ratio of propane-air mixtures. In the co-feed mode, the combustion characteristics of hydrogen-assisted propane qualitatively resemble the selectively preheating initial portion of the combustion chamber wall. In the sequential feed mode, the time taken to reach steady state, the hydrogen cut-off time, the propane ignition time and the cumulative propane emissions increased with increasing wall thermal conductivity; the ignition characteristics are similar to partially preheating the initial segment for low and moderate wall thermal conductivity values (0.5 and 20 W/m·K); however, the ignition characteristics are close to completely heating the micro-combustor wall for high wall thermal conductivity values (200 W/m·K).

Systematic review of effects of pregnancy on breast and abdominal contour after TRAM/DIEP breast reconstruction in breast cancer survivors.

Transverse rectus abdominis myocutaneous (TRAM) and its derivatives are the most commonly performed autologous breast reconstruction procedures. These procedures were not recommended in the past for those who planned for subsequent childbearing because of the transposition of portions of the abdominal wall during the procedure into the anatomic position of the breast, implying possible adverse effects over the contour of these manipulated areas during pregnancy and delivery. We conducted a systematic review to assess the literature on esthetic or functional consequences of childbearing over the breast and abdomen after these procedures in patients that were affected by breast cancer. A comprehensive literature search in databases and citation indexes was conducted from February 2014 to April 2015. Any paper on pregnancy after breast reconstruction by TRAM or its derivatives and modifications, written in English or French, were included. The search results underwent a first screening to exclude duplicate and irrelevant papers. Full texts were then reviewed as to the criteria for inclusion, and data were extracted into data extraction forms from eligible papers. The initial search yielded 5132 articles. After screening and review, overall 17 papers met all criteria for inclusion in this review. Our work revealed that uneventful pregnancy and delivery can be anticipated in breast cancer survivors who had undergone breast reconstruction via TRAM or its derivatives with minor negative effects on either the breast or the abdomen.

Cooling of Heat Sources by Natural Convection Heat Transfer in a Vertical Annulus

This article reports convection heat transfer in a short and tall annular enclosure with two discrete isoflux heat sources of different lengths. The discrete heat sources are mounted at the inner wall and the outer wall is maintained at a lower temperature, whereas the top and bottom walls and the unheated portions of the inner wall are kept at adiabatic. An implicit finite-difference method is employed to solve the vorticity–stream function formulations of the governing equations. The significant influence of the discrete heaters on the flow and heat transfer is analyzed for a wide range of modified Rayleigh numbers, aspect ratio, and length ratio (ϵ) of heat sources. Our numerical results reveal that the average Nusselt number decreases with aspect ratio, whereas the magnitude of maximum temperature increases with the aspect ratio. For most of the parametric cases considered in the present study, the heat transfer rate is found to be higher at the bottom heater than at the top heater except for ϵ = 0.5. The effect of heater length ratio on the heat transfer rate is noticeable for unit aspect ratio, whereas its effect is insignificant as the aspect ratio increases. Furthermore, it was found that the maximum temperature is found generally at the top heater except for the case ϵ = 0.5, where the maximum temperature is found at the bottom heater.

Technique for Repair of Fractures and Separations Involving the Cartilaginous Portions of the Anterior Chest Wall

Internal fixation of the ribs has been shown in numerous studies to decrease complications following traumatic rib fractures. Anterior injuries to the chest wall causing cartilaginous fractures, although rare, can cause significant disability and can lead to a variety of complications and, therefore, pose a unique clinical problem. Here, we report the surgical technique used for four patients with internal fixation of injuries to the cartilaginous portions of the chest wall treated at our center. All patients had excellent clinical outcomes and reported improvement in symptoms, with no associated complications. Patients who have injuries to the anterior portions of the chest wall should be considered for internal fixation of the chest wall when the injuries are severe and can lead to clinical disability.

SU‐E‐P‐42: Benefit of Equivalent Uniform Dose in Prostate IMRT Planning to Reduce Bladder Toxicity

Purpose:To assess the benefit of bladder wall sub‐volume equivalent uniform dose (EUD) constrains in prostate cancer IMRT planningMethods:The bladder wall was defined by the volume between the external manually delineated wall and a contraction of 7 mm from this external wall. This bladder wall was then separated into two parts: the internal‐bladder wall (bla‐in) represented by the portion of the bladder wall that intersected with the planning target volume (PTV) plus 5 mm extension; the external‐bladder wall (bla‐ex) represented by the remaining part of the bladder wall. Two IMRT plans, with and without using EUD constraints, were generated and compared for 53 prostate cancer patients, to deliver 80Gy to the prostate PTV (V95&gt;95%). In the plan with EUD constraints, the values of the “a” parameter of the EUD models were: 10.0 for bla‐in and 2.3 for bla‐ex., and 5 for the rectum.Results:The use of bladder EUD objectives increased significantly the conformal index (0.73±0.04 vs. 0.93±0.02) and decreased both the doses in the bladder wall (V70: 22.66% vs. 18.88%, Dmean: 39.40Gy vs. 35.04Gy) and the bladder wall NTCP values (NTCP at 3 years bladder toxicity &gt;= LENT/SOMA Grade2: 16.68% vs. 14.48%, NTCP at 3 years bladder bleeding: 7.16% vs. 5.88%, NTCP at 5 years bladder toxicity &gt;= LENT/SOMA Grade2: 20.15% vs. 17.75%, NTCP at 5 years bladder bleeding: 10.38% vs. 8.36%). The use of bladder EUD objectives, although slightly decreasing the dose to the rectum wall (Dmax: 75.19Gy vs. 75.05Gy, V72: 12.95Gy vs. 12.72Gy) as well, increased however the dose in the femoral heads (V55‐femoral head left: 0.06% vs. 0.30%, V55‐femoral head right: 0.04% vs. 0.21%).Conclusion:Separating bladder wall into two parts with appropriate bladder EUD objectives may reduce bladder toxicity, while keeping high dose to the prostate.

Steady and oscillatory laminar opposing mixed convection in a vertical channel of finite length subjected to symmetrical isothermal discrete heat sources

Transient laminar opposing mixed convection in a gravity driven downward flow confined inside a vertical rectangular channel has been investigated, with both walls suddenly subjected to symmetrical isothermal heat sources over a finite portion of the channel walls. The unsteady two-dimensional Navier-Stokes and energy equations have been solved numerically for a wide parametric set. Studies are carried out for Reynolds numbers of 100 and 200 and several values of buoyancy strength or Richardson number. The effect of Reynolds number and opposing buoyancy on the temporal evolution of the overall flow structure, temperature field, and Nusselt number from the heated surfaces is investigated using fixed geometrical parameters and considering heat losses to the channel walls. In this parameter space, for a given Reynolds number and relatively small values of the buoyancy parameter, the transient process leads to a final symmetric or asymmetric steady-state. However, as the value of buoyancy strength increases, the flow and temperature fields become more complex and an oscillatory flow with a fundamental frequency sets in when a critical value of the Richardson number is reached. Numerical predictions show that the critical value of the Richardson number between the two regimes strongly depends on the value of the Reynolds number, and the time scales, natural frequencies, and phase-space portraits of flow oscillation are presented and discussed in detail. Stability of the symmetric response has been analyzed. The results include the effects of Prandtl number and heat losses to the channel walls on the evolution of the final flow and thermal responses.

Extensive regeneration of the stomach using bioabsorbable polymer sheets

The growing prevalence of endoscopic surgery in recent years has led to the minimization of postoperative scarring. However, this procedure does not allow for the regeneration of the resected digestive tract, which compromises the postoperative maintenance of digestive function. In this preliminary study, we developed an artificial gastric wall (AGW) using bioabsorbable polymer (BAP), and evaluated the ability of this BAP patch to repair and regenerate a widely defective gastric wall in an animal model. Pigs were laparotomized under general anesthesia. An 8 × 8-cm, round portion of the anterior gastric wall was excised and replaced by an AGW. The AGW was composed of a copolymer comprising 50% lactic acid and 50% caprolactone. The animals were relaparotomized 4, 8, or 12 weeks after implantation, after which they underwent resection of the entire stomach for gross and histologic evaluation of the graft sites. All recipient pigs survived until killing. By 4-8 weeks, the graft site revealed progressively fewer mucosal defect after each day. Moreover, the grafted area was indistinguishable from the native stomach 12 weeks after AGW implantation. The structures of the regenerated mucous membrane and muscle layers were identical to those of the native stomach. Furthermore, proton pumps were found in the regenerated tissue. The BAP sheets helped to restore extensive gastric defects without causing any deformation. The use of BAP sheets may become a new therapeutic method that prevents alterations of gastric volume after extensive gastrectomy for stomach cancer and other diseases.

Multi-Channel Vaginal Cylinder Brachytherapy: Impact of Tumor Size and Location on Dose to Organs at Risk

Multichannel vaginal brachytherapy (MCVB) allows dose to be preferentially delivered to particular portions of the vaginal side wall while sparing other regions. For superficial lesions MCVB has been used as an alternative to interstitial brachytherapy and can be thought of as an extension of intracavitary brachytherapy. Due to the exponential dose fall off associated with brachytherapy, treating lesions of increasing depth requires exponentially higher doses to more proximal tissue. The aim of this study was to investigate the dosimetric consequences of treating lesions of varying size and location with a multi-channel vaginal cylinder. We identified patients previously treated with MCVB and randomly selected one patient for each of six categories based on location (lateral, anterior, or vaginal cuff/apex) and size of cylinder (2.5 cm or 3.0 cm). Based on MRI, each patient’s target lesion was extended circumferentially into theoretical high risk clinical target volumes (HRCTV) measuring 5 mm, 7 mm, and 10 mm in depth. Rectum and bladder were contoured for each patient. Using Nucletron Oncentra version 4.0 (Nucletron, Veneendal Netherlands), image-based brachytherapy treatment plans for each of the six patients’ three target volumes were generated. Each plan was required to have a D90 of 100%. Total dose EQD2 dose was calculated using an EBRT dose of 45 Gy in 25 fractions in conjunction with a high dose rate (HDR) brachytherapy dose of 25 Gy in five fractions. Vaginal surface was contoured by creating a 1 mm expansion from the cylinder surface and subtracting out the cylinder. Maximum EQD2 vaginal surface doses in gray for 5 mm/7 mm/10 mm targets were as follows (location-cylinder size): lateral-3.0 cm:122/153/210, lateral-2.5 cm:145/195/301, anterior-3.0 cm:115/135/197, anterior-2.5 cm:132/173/283, apex-3.0 cm:173/241/367, apex-2.5 cm: 349/461/706. This represents an average increase of 121 Gy to the vaginal surface when moving from a 5 mm thick lesion to a 10 mm thick lesion and treating with a 3 cm cylinder and 221 Gy for a 2.5 cm cylinder. Across all 18 plans, total EQD2 dose to 2 cc of the rectum ranged from 53.9-67.2 Gy. There was an average 3.44 Gy (range=0.83-6.01 Gy) absolute increase in rectal 2 cc EQD2 total dose when target depth increased from 5 mm to 10 mm. Total EQD2 bladder 2 cc dose ranged from 51.5-71.2 Gy. There was an average 5.24 Gy (range=0.66-9.99 Gy) absolute increase in total EQD2 bladder 2 cc dose when target depth increased from 5 mm to 10 mm. The vaginal surface dose appears to be the dose-limiting structure for anterior, lateral, and apical vaginal lesions. Caution should be taken when treating apical lesions and lesions greater than 5mm in depth especially with cylinders smaller than 3.0 cm in diameter. In such cases, interstitial brachytherapy should be given strong consideration.

Generalized Minimal Principle for Rotor Filaments

To a reaction-diffusion medium with an inhomogeneous anisotropic diffusion tensor D, we add a fourth spatial dimension such that the determinant of the diffusion tensor is constant in four dimensions. We propose a generalized minimal principle for rotor filaments, stating that the scroll wave filament strives to minimize its surface area in the higher-dimensional space. As a consequence, stationary scroll wave filaments in the original 3D medium are geodesic curves with respect to the metric tensor G=det(D)D(-1). The theory is confirmed by numerical simulations for positive and negative filament tension and a model with a non-stationary spiral core. We conclude that filaments in cardiac tissue with positive tension preferentially reside or anchor in regions where cardiac cells are less interconnected, such as portions of the cardiac wall with a large number of cleavage planes.

Viscous flow past a collapsible channel as a model for self-excited oscillation of blood vessels

Motivated by collapse of blood vessels for both healthy and diseased situations under various circumstances in human body, we have performed computational studies on an incompressible viscous fluid past a rigid channel with part of its upper wall being replaced by a deformable beam. The Navier–Stokes equations governing the fluid flow are solved by a multi-block lattice Boltzmann method and the structural equation governing the elastic beam motion by a finite difference method. The mutual coupling of the fluid and solid is realized by the momentum exchange scheme. The present study focuses on the influences of the dimensionless parameters controlling the fluid–structure system on the collapse and self-excited oscillation of the beam and fluid dynamics downstream. The major conclusions obtained in this study are described as follows. The self-excited oscillation can be intrigued by application of an external pressure on the elastic portion of the channel and the part of the beam having the largest deformation tends to occur always towards the end portion of the deformable wall. The blood pressure and wall shear stress undergo significant variations near the portion of the greatest oscillation. The stretching motion has the most contribution to the total potential elastic energy of the oscillating beam.

Mesodermal gene expression during the embryonic and larval development of the articulate brachiopod Terebratalia transversa.

BackgroundBrachiopods undergo radial cleavage, which is distinct from the stereotyped development of closely related spiralian taxa. The mesoderm has been inferred to derive from the archenteron walls following gastrulation, and the primary mesoderm derivative in the larva is a complex musculature. To investigate the specification and differentiation of the mesoderm in the articulate brachiopod Terebratalia transversa, we have identified orthologs of genes involved in mesoderm development in other taxa and investigated their spatial and temporal expression during the embryonic and larval development of T. transversa.ResultsOrthologs of 17 developmental regulatory genes with roles in the development of the mesoderm in other bilaterian animals were found to be expressed in the developing mesoderm of T. transversa. Five genes, Tt.twist, Tt.GATA456, Tt.dachshund, Tt.mPrx, and Tt.NK1, were found to have expression throughout the archenteron wall at the radial gastrula stage, shortly after the initiation of gastrulation. Three additional genes, Tt.Pax1/9, Tt.MyoD, and Tt.Six1/2, showed expression at this stage in only a portion of the archenteron wall. Tt.eya, Tt.FoxC, Tt.FoxF, Tt.Mox, Tt.paraxis, Tt.Limpet, and Tt.Mef2 all showed initial mesodermal expression during later gastrula or early larval stages. At the late larval stage, Tt.dachshund, Tt.Limpet, and Tt.Mef2 showed expression in nearly all mesoderm cells, while all other genes were localized to specific regions of the mesoderm. Tt.FoxD and Tt.noggin both showed expression in the ventral mesoderm at the larval stages, with gastrula expression patterns in the archenteron roof and blastopore lip, respectively.ConclusionsExpression analyses support conserved roles for developmental regulators in the specification and differentiation of the mesoderm during the development of T. transversa. Expression of multiple mesodermal factors in the archenteron wall during gastrulation supports previous morphological observations that this region gives rise to larval mesoderm. Localized expression domains during gastrulation and larval development evidence early regionalization of the mesoderm and provide a basis for hypotheses regarding the molecular regulation underlying the complex system of musculature observed in the larva.Electronic supplementary materialThe online version of this article (doi:10.1186/s13227-015-0004-8) contains supplementary material, which is available to authorized users.

Mast cells in the colon of Trypanosoma cruzi-infected patients: are they involved in the recruitment, survival and/or activation of eosinophils?

Megacolon is frequently observed in patients who develop the digestive form of Chagas disease. It is characterized by dilation of the rectum-sigmoid portion and thickening of the colon wall. Microscopically, the affected organ presents denervation, which has been considered as consequence of an inflammatory process that begins at the acute phase and persists in the chronic phase of infection. Inflammatory infiltrates are composed of lymphocytes, macrophages, natural killer cells, mast cells, and eosinophils. In this study, we hypothesized that mast cells producing tryptase could influence the migration and the activation of eosinophils at the site, thereby contributing to the immunopathology of the chronic phase. We seek evidence of interactions between mast cells and eosinophils through (1) evaluation of eosinophils, regarding the expression of PAR2, a tryptase receptor; (2) correlation analysis between densities of mast cells and eosinophils; and (3) ultrastructural studies. The electron microscopy studies revealed signs of activation of mast cells and eosinophils, as well as physical interaction between these cells. Immunohistochemistry and correlation analyses point to the participation of tryptase immunoreactive mast cells in the migration and/or survival of eosinophils at the affected organ.

When Closure Fails: What the Radiologist Needs to Know About the Embryology, Anatomy, and Prenatal Imaging of Ventral Body Wall Defects

Ventral body wall defects (VBWDs) are one of the main categories of human congenital malformations, representing a wide and heterogeneous group of defects sharing a common feature, that is, herniation of one or more viscera through a defect in the anterior body wall. Gastroschisis and omphalocele are the 2 most common congenital VBWDs. Other uncommon anomalies include ectopia cordis and pentalogy of Cantrell, limb-body wall complex, and bladder and cloacal exstrophy. Although VBWDs are associated with multiple abnormalities with distinct embryological origins and that may affect virtually any system organs, at least in relation to anterior body wall defects, they are thought (except for omphalocele) to share a common embryologic mechanism, that is, a failure involving the lateral body wall folds responsible for closing the thoracic, abdominal, and pelvic portions of the ventral body wall during the fourth week of development. Additionally, many of the principles of diagnosis and management are similar for these conditions. Fetal ultrasound (US) in prenatal care allows the diagnosis of most of such defects with subsequent opportunities for parental counseling and optimal perinatal management. Fetal magnetic resonance imaging may be an adjunct to US, providing global and detailed anatomical information, assessing the extent of defects, and also helping to confirm the diagnosis in equivocal cases. Prenatal imaging features of VBWDs may be complex and challenging, often requiring from the radiologist a high level of suspicion and familiarity with the imaging patterns. Because an appropriate management is dependent on an accurate diagnosis and assessment of defects, radiologists should be able to recognize and distinguish between the different VBWDs and their associated anomalies. In this article, we review the relevant embryology of VBWDs to facilitate understanding of the pathologic anatomy and diagnostic imaging approach. Features will be illustrated with prenatal US and magnetic resonance imaging and correlated with postnatal and clinical imaging.

A phreatic explosion model inferred from a very long period seismic event at Mayon Volcano, Philippines

AbstractDuring a phreatic explosion at Mayon Volcano, Philippines, on 7 May 2013, a very long period seismic event with a peak frequency of 0.4 Hz was observed. Our frequency‐domain waveform inversion solution of the event in the frequency range 0.1–0.6 Hz is consistent with a subhorizontal tensile crack and a vertical single force at a shallow location beneath the summit crater. The source time functions obtained by the waveform inversion are band‐passed forms. We estimated the deconvolved forms of the source time functions (DSTFs), which are source time functions corrected for the effects of the band‐pass filter. The DSTF of the crack can be approximated by an impulse‐type function composed of inflation followed by deflation, whereas the DSTF of the single force can be approximated by a downward impulse. The inflation of the crack may be attributed to boiling of underground water and its deflation can be attributed to discharge of water vapor, whereas the downward force may be understood as the counterforce of the explosion. Our results suggest that only a portion of the crack wall was destroyed by the explosion. We could not find clear precursors in seismic, thermography, geothermal, geodetic, and meteorological data. We present a model of repeated explosions in which an explosion can occur once the fragmented portion of the crack is sealed by precipitation of clay minerals or hydrothermal secondary deposits. This model may explain the absence of clear precursory signals before the 2013 explosion.

Cytochemical, Structural and Ultrastructural Characterization of Tetrasporogenesis in Bostrychia radicans (Ceramiales, Rhodophyta) from the Mangroves of Itacorubi and Rio Ratones, Santa Catarina, Brazil

Little is known about the morphology and location of macromolecules, especially proteins and carbohydrates, in vegetative and reproductive structures of mangrove species, including Bostrychia radicans. Therefore, to gain a better understanding of tetrasporogenesis in B. radicans, cytochemical, structural and ultrastructual analyses were performed. Thalli were collected from mangroves in Florianópolis, Santa Catarina, Brazil. Fertile branches were fixed and processed for light microscopy (LM), confocal microcopy and transmission electron microscopy (TEM) observations. The LM sections were stained with toluidine blue, periodic acid-Schiff and Coomassie brilliant blue. Tetrasporogenesis occurs in the pericentral cells of the terminal branches. This process is initially characterized by an increase in cell volume, resulting from the proliferation of organelles. The young tetrasporangia remain connected to the basal cell by pit connections. After a considerable increase in volume, the tetrasporocytes divide tetrahedrally, giving rise to haploid spores. During this process, there is an increasing production of starch grains, causing the organelles to group. As the organelles proliferate, the plasma membrane undergoes simultaneous invaginations toward the tetrasporangium center. The most conspicuous organelle throughout tetrasporogenesis was the Golgi complex. Polysaccharidic components are predominant in the tetrasporangium cell wall throughout tetrasporogenesis. Although protein components prevail in the cytoplasm of younger tetrasporangia, there is a predominance of reserve material with maturation. In the initial phase, there is an increase in the number of chloroplasts and a significant increment of Golgi bodies which contribute to the formation of the amorphous portion of the cell wall and possibly the biosynthesis of starch grains.

A scanning electron microscopy evaluation of the cleanliness of un-instrumented areas of canal walls after root canal preparation

Introduction: Cleanliness of the canal space is the ultimate goal of its preparation. Nevertheless, some portion of the canal walls are left un-instrumented during preparation. Therefore, the aim of this ex vivo study was to evaluate the cleanliness of un-instrumented canal walls after root canal preparation for the presence or absence of debris and smear layer. Materials and Methods: A total of 24 single-rooted extracted teeth were prepared with the crown-down technique using Protaper universal rotary file system. Micro-computed tomography (micro-CT) was used to scan the specimens before and after instrumentation. The un-instrumented area was measured and localized. The roots were split longitudinally and then subjected to scanning electron microscopy (SEM). The presence of debris and smear layer in the instrumented and un-instrumented areas of the canal were evaluated by analysing the SEM images with a five-score evaluation system based on the reference photographs. Results: High levels of root canal cleanliness (≤ score 2) were found for the instrumented areas were detected (P = 0.003). Conclusions: Under the condition of this study, un-instrumented areas of the canal were less clean in comparison to instrumented portion.

Primary Cold Abscess of Rectus Abdominis Muscle

A 20-year-old female presented with a complaint of a progressive swelling in the left lower abdomen for the last three months. There was no history of preceding trauma, fever, cough, malaise or pain. There was no history of contact with any case of tuberculosis. On examination, there was swelling in the left iliac fossa measuring 8 × 8 cm in size, non-tender with smooth and ill-defined margins and a normal overlying skin. Examinations of the cardiovascular and respiratory system were within normal limits. Laboratory investigations were within normal limits. ELISA for HIV was negative. The chest radiograph was unremarkable. Abdominal ultrasonography of the abdomen revealed a 6.5 × 8.5 cm left iliac fossa cystic mass with a liquefied necrotic center in the anterior abdominal wall (Figure 1A). Computerized Tomography scan of the abdomen showed an abscess in the left antero-lateral portion of the abdominal wall limited to the muscle layer (Figure 1B). Ultrasound-guided fine-needle aspiration and cytological examination revealed caseating granuloma with central necrosis, lymphocytes, and giant cells, consistent with tuberculosis (Figure 1C). The patient was diagnosed to have tuberculous abscess of the anterior abdominal wall and antituberculosis treatment was started. After four weeks’ antituberculous treatment, she responded well to the treatment and the abscess regressed considerably. The prognosis is good in tuberculous myositis with appropriate chemotherapy [1,2]. This case cautions the clinicians and radiologists about the possibility of tuberculosis in considering the differential diagnosis of any lesion even in any unlikely anatomical area, especially in those areas where tuberculosis is endemic.