Portable Diagnostic Tool Research Articles

Effects of different capture techniques on the physiological condition of bonefishAlbula vulpesevaluated using field diagnostic tools

A study was conducted on bonefishAlbula vulpesin The Bahamas to assess the utility of portable physiological diagnostic tools (i‐STAT analyser with E3+ ion and haematology cartridge, ACCU‐CHEK glucose meter and Lactate Pro lactate meter) for field physiology applications in remote locations. Physiological values derived from portable diagnostic tools were significantly related to values obtained from standard laboratory techniques [glucose (r2= 0·96), packed cell volume (PCV;r2= 0·33), Na+(r2= 0·28), K+(r2= 0·71) and Cl−(r2= 0·15)]. Actual values (i.e.intercepts), however, tended to deviate slightly between the two techniques. Nonetheless, these tools showed promise for documenting relative differences among fishes experimentally exposed to treatments inducing different levels of ‘stress’. These tools were then used to characterize the effects of different capture techniques on the stress response ofA. vulpes.Albula vulpescaptured in seines and then temporarily held in pens were physiologically sampled between 1 and 45 min postcapture to evaluate postcapture stress dynamics. Blood glucose and lactate as well as PCV and haemoglobin (Hb) increased rapidly after capture but stabilized at maximal values byc.20 min postcapture. When angled, largerA. vulpestook longer to exhaust and land than did smaller individuals. In addition, there was a positive relationship between the magnitude of increase in lactate and the duration of the angling event, implying that anglers can reduce stress by minimizing the duration of the fight. Fish sampled before and after a simulated angling treatment displayed clear increases in blood lactate, K+, PCV and Hb, providing some of the first data on how individualA. vulpesrespond to angling stress. In summary, this study revealed that techniques are now available for conducting field physiological studies onA. vulpesand possibly other species in remote locales, and that haematological and biochemical indicators of physiological disturbance vary with the intensity of the angling event.

The Potential of a Chlorophyll Content SPAD Meter to Quantify Nutrient Stress in Foliar Tissue of Sycamore (Acer pseudoplatanus), English Oak (Quercus robur), and European Beech (Fagus sylvatica)

The chlorophyll content (or SPAD meter) is a simple, portable diagnostic tool that measures the greenness or relative chlorophyll content of leaves. Compared with the traditional destructive methods of chlorophyll extraction, the use of this equipment saves time, space, and resources. The objective of this study was to establish a correlation between the leaf photosynthetic pigment content (chlorophylls, carotenoids) extracted in aqueous acetone, total leaf nitrogen (N) content, and chlorophyll fluorescence Fv/Fm values with the SPAD-502 readings in sycamore (Acer pseudoplatanus), beech (Fagus sylvatica), and English oak (Quercus robur) leaves displaying visual symptoms of N deficiency. In addition, this study aimed to determine a critical foliar N content below which a reduction in photosynthetic efficiency occurs. Irrespective of species, high correlations were recorded between SPAD readings, total leaf chlorophyll and carotenoid content, foliar N content, and leaf photosynthetic efficiency as measured by chlorophyll fluorescence Fv/Fm values; however, a poor correlation between SPAD values and total chlorophyll:carotenoid ratios was obtained. In the case of Acer pseudoplatanus, Fagus sylvatica, and Quercus robur, SPAD readings lower than 25 indicated impairment of leaf photosynthetic process that in turn were correlated with a foliar N content less than 1.5%, a value associated with a critical N deficiency. Results of this study indicate that the chlorophyll content SPAD-502 m potentially offers a useful nondestructive, handheld system to aid in the evaluation of tree health. However, users should be aware of the limitations of this system. Consistency in sample collection and seasonal timing may necessitate species and cultivar calibration equations to correlate SPAD values with reductions in tree vitality.

Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves

The chlorophyll meter (SPAD-502) is a simple, portable diagnostic tool that measures the greenness or relative content of leaves. Compared to the traditional destructive methods, the use of this equipment saves time, space and resources. The objective of this study was to establish a correlation between the photosynthetic pigments content extracted in DMSO, the total nitrogen content and the chlorophyll a fluorescence variables with the SPAD-502 readings in Coffea canephora Pierre leaves. The SPAD-502 has been shown to be a good tool to diagnose the integrity of the photosynthetic system in coffee leaves, and can thus help in the advanced interpretations of the photochemical process of these plants. The SPAD readings lower 40 show impairment in photosynthetic process. Thus, the portable chlorophyll SPAD-502 can be used to analyze the photosynthetic pigments, and total nitrogen can also help in interpretation of the photochemical process in coffee plants.

Chlorophyll Meter– and Leaf Color Chart–Based Nitrogen Management for Rice and Wheat in Northwestern India

Overapplication of N in cereal crops leads to low N recovery efficiency and risk of NO3 pollution of ground water. The chlorophyll meter, also known as SPAD meter, is a simple, portable diagnostic tool for identifying crop N status. We used it to test need‐based N management approaches for rice (Oryza sativa L.) and wheat (Triticum aestivum L.) on a loamy sand in northwestern India. Applying 30 kg N ha−1 each time the SPAD value fell below the critical value of 37.5 resulted in application of 90 kg N ha−1, which produced rice yields equivalent to those with 120 kg N ha−1 applied in three splits. Using a SPAD value of 35 was inadequate for the two rice cultivars because it resulted in application of only 60 kg N ha−1 and, thus, low yields. With high inherent soil fertility resulting in rice yield of >3 Mg ha−1 in zero‐N plots, applying N basally or a week after rice transplanting did not further increase yield. Limited experimentation with leaf color chart (LCC) indicated that N management based on LCC shade 4 helped avoid overapplication of N to rice. Wheat responded to N application at maximum tillering (MT) when SPAD value fell below 44. Wheat yield increased by 20% when 30 kg N ha−1 was applied at SPAD value of 42 at MT. Results show that plant need–based N management through chlorophyll meter reduces N requirement of rice from 12.5 to 25%, with no loss in yield.

Chlorophyll Meter– and Leaf Color Chart–Based Nitrogen Management for Rice and Wheat in Northwestern India

Overapplication of N in cereal crops leads to low N recovery efficiency and risk of NO3 pollution of ground water. The chlorophyll meter, also known as SPAD meter, is a simple, portable diagnostic tool for identifying crop N status. We used it to test need-based N management approaches for rice (Oryza sativa L.) and wheat (Triticum aestivum L.) on a loamy sand in northwestern India. Applying 30 kg N ha−1 each time the SPAD value fell below the critical value of 37.5 resulted in application of 90 kg N ha−1, which produced rice yields equivalent to those with 120 kg N ha−1 applied in three splits. Using a SPAD value of 35 was inadequate for the two rice cultivars because it resulted in application of only 60 kg N ha−1 and, thus, low yields. With high inherent soil fertility resulting in rice yield of >3 Mg ha−1 in zero-N plots, applying N basally or a week after rice transplanting did not further increase yield. Limited experimentation with leaf color chart (LCC) indicated that N management based on LCC shade 4 helped avoid overapplication of N to rice. Wheat responded to N application at maximum tillering (MT) when SPAD value fell below 44. Wheat yield increased by 20% when 30 kg N ha−1 was applied at SPAD value of 42 at MT. Results show that plant need–based N management through chlorophyll meter reduces N requirement of rice from 12.5 to 25%, with no loss in yield.