Pork Backfat Research Articles

Peanut and Soy Protein-Based Emulsion Gels Loaded with Curcumin as a New Fat Substitute in Sausages: A Comparative Study

The aim of this study was to evaluate the effects of the complete or partial substitution (0, 20, 40, and 100%) of the pork backfat in prepared sausage with protein emulsion gels loaded with curcumin. The effects of three protein emulsion gels (i.e., peanut proteins, ultrasound-modified peanut proteins, and soy proteins) on sausage characteristics (cooking loss, textural properties, microstructure, sensory characteristics, and antioxidant activity) were investigated and compared using a one-way analysis of variance and Duncan’s multiple tests. The results revealed that the addition of each emulsion gel reduced cooking loss and improved the textural properties of the sausages in a dose-dependent manner. When 20% of pork backfat was substituted with untreated or ultrasound-modified peanut protein emulsion gel (PPEG), cooking loss decreased to a greater extent than when soy protein emulsion gel (SPEG) was used. However, the latter yielded higher cohesiveness and resilience at the same substitution levels. Compared with untreated PPEG, the sausages containing modified PPEG (at 200 W for 20 min) had significantly greater resilience and a denser microstructure. In addition, when 100% of pork backfat was substituted with modified PPEG, the sausages had desirable sensory characteristics. All sausages enriched with protein emulsion gels loaded with curcumin presented higher DPPH and ABTS radical scavenging capacities than the control sausages. The sausages prepared with the modified PPEG had the highest antioxidant activity (DPPH: 37.43 ± 0.35%; ABTS: 39.48 ± 0.50%; TBARS: 0.65 ± 0.05 mg MDA/Kg), which may be attributed to the increased stability of curcumin in the modified PPEG with a denser network structure. Therefore, ultrasound-modified PPEG loaded with curcumin can be used as a new fat substitute in functional sausages or other healthy meat products.

Pickering emulsion stabilized by ergosterol solely as a new fat substitute improves the quality of pork sausages

The present study aimed to develop healthier sausages by utilizing ergosterol-stabilized Pickering emulsion as a substitute for animal fat. In this context, a Pickering emulsion stabilized with ergosterol was developed, and its impact on the sausage quality was investigated by replacing 25%–100% of pork backfat. Our findings revealed that the W/O emulsion exhibited superior freeze-thaw stability. All meat pastes displayed distinct gel-like rheological behavior, with the highest values observed at 50% fat substitution. Notably, sausages reformulated with 50% emulsion showed enhanced uniformity and compactness, resulting in a significant reduction in cooking loss, L∗ values, and b∗ values, while exhibiting a notable increase in a∗ values. The texture properties reached their maximum at 50% fat substitution. Furthermore, 50% fat replacement decreased the relaxation time of T2b and increased the proportion of bound and immobilized water. Reformulation with 50% emulsion not only generated novel flavor substances but also led to an increased PUFA/SFA ratio as well as decreased atherogenic index and thrombogenic index. In conclusion, reformulating with 50% emulsion significantly improved the physicochemical characteristics and fatty acid composition of pork sausages, which highlights the promising potential of ergosterol-stabilized Pickering emulsion as an effective alternative for fats in meat products.

Biopolymeric emulsions with added silicon as pork fat substitutes in the reformulation of healthy pâtés: Effect on technological, nutritional and sensory properties and on lipid digestibility after in vitro gastrointestinal digestion

Four different pork lard emulsions were elaborated in order to be used as pork backfat replacers in the reformulation of healthy pâtés: an emulsion stabilized with soy protein concentrate (SPC, E1), E1 with added silicon (Si) (E2), E1 with a mixture of methylcellulose (MC) and carboxymethylcellulose (CMC) (E3), and E3 with added Si (E4). These four emulsions were characterized in terms of texture, color and in vitro gastrointestinal digestion (GID) and were then used in the reformulation of four pork liver pâtés (PE1-PE4). These pâtés were compared to a control pâté elaborated with 100% pork backfat (PC). The technological, nutritional and sensory properties of the pâtés were assessed, along with lipid digestibility based on the content of free fatty acids (FFAs) released after in vitro GID. The reformulated pâtés had highly adequate physicochemical and sensory properties, comparable to those of the PC control. The addition of a mixture of MC/CMC and Si in the SPC-stabilized emulsion caused a slow down of in vitro GID and, consequently, lower lipid bioaccessibility (BA) of the main fatty acids when E3 and E4 were incorporated into the reformulated pâtés PE3 and PE4. Si BA was higher in the absence of cellulose ethers (14.6%) that in their presence (8.84%).

Physical, Chemical, and Sensory Characteristics of Chicken Sausages Using Corn Oil Instead of Pork Back Fat

Physical, Chemical, and Sensory Characteristics of Chicken Sausages Using Corn Oil Instead of Pork Back Fat

Blackcurrant Pomace Extract as a Natural Antioxidant in Vienna Sausages Reformulated by Replacement of Pork Backfat with Emulsion Gels Based on High Oleic Sunflower and Flaxseed Oils.

The incorporation of a blackcurrant pomace extract (BPE) at 2.5%, 5.0% and 10.0% into an emulsion gel based on high oleic sunflower and linseed oils was examined in order to obtain a functional ingredient to be used as a pork backfat replacer in Vienna sausages. The replacement of the pork backfat with the control emulsion gel reduced the cooking loss but negatively affected the color by decreasing L* and a* values as compared with the traditional product. A decrease in the n-6/n-3 ratio from 10.99 to around 1.54 (by 7 times) was achieved through reformulation, while the PUFA/SFA ratio increased from 0.49 to 1.09. The incorporation of BPE did not have a major impact on the fatty acid profile and improved color by increasing redness, but negatively affected the texture by increasing hardness, gumminess and share force as compared with the sausages reformulated without extract. BPE reduced the pH and the thermal stability of the emulsion gels, increased cooking loss and decreased moisture retention in sausages. BPE increased the oxidative stability of Vienna sausages enriched in polyunsaturated fatty acids; however, the incorporation of BPE into the emulsion gels above 5% affected the sensory scores for appearance, texture and general acceptability of the reformulated sausages.

Use of Different O/W or W/O Emulsions as Functional Ingredients to Reduce Fat Content and Improve Lipid Profile in Spanish Cured Processed Meat Product ('Chorizo').

In this work, three out of five types of oil-in-water and water-in-oil emulsions were selected to replace pork backfat to reduce the fat content and the improve monounsaturated and polyunsaturated fatty acid content in dry cured sausage ('chorizo'). Different characteristics of the new product were studied: the texture profile, color, nutritional value, lipid profile, vitamin E and thiobarbituric acid (TBA) and sensory qualities. The use of emulsions to replace the animal fat affected all color parameters, obtaining darker, less red and yellow products, which could impact the consumer's purchase intention. This replacement also altered the texture parameters, increasing or decreasing the hardness in comparison with the control sample. The cohesiveness, however, decreased in all cases, which meant that when the samples are cut for consumption, they disintegrated more than the traditional ones. The most relevant aspect for nutritional value is that the use of the new emulsions helped to reduce the total fat and energy value of the reformulated samples. The most significant aspect is that this reformulation helped to reduce the fat content-specifically, saturated fat-while increasing the content of Omega 3/6. The new formulas contained significant quantities of TBA and vitamin E when comparing them with the traditional product.

Candelilla Wax and Glycerol Monostearate-Based Oleogels as Animal Fat Substitutes in Bologna Sausages.

The aim of this study was to produce Bologna sausages rich in unsaturated fatty acids and to evaluate this replacement on the structural characteristics. For the purpose of a comparative analysis, three different types of sausages were produced, distinct only in the type of fat used: I. sausages obtained with pork backfat (PBF), II. sausages produced with oleogel formed from refined sunflower oil and glycerol monostearate (GM_OG), and III. with candelilla wax oleogel (CW_OG). The meat composition was also analyzed to better understand the process in the dynamics and the finished products were analyzed both uncooked and cooked. The enhanced oil-binding capacity of oleogels suggests their potential value as substitutes for saturated fats (>99%). In terms of meat composition textural analysis, the highest hardness value was registered for PBF_C of 25.23 N, followed by a CW_OG_C of 13.08 N and a GM_OG_C of 12.27 N. However, adhesiveness, cohesiveness, springiness index, and gumminess showed similar values between samples. Reformulation of products with oleogels as a fat source abundant in mono- and polyunsaturated fatty acids resulted in uncooked products exhibiting reduced hardness values of 49.01 N (CW_OG_US) and 40.51 N (GM_OG_US), compared to 65.03 N (PBF_US). Color results of the cross-section color can indicate the potential for consumer acceptance due to the reduced color differences between the conventional and oleogel samples.

Fatty Acid Profile and Thermal Behavior of Fat-Rich Edible Insect Oils Compared to Commonly Consumed Animal and Plant Oils.

This study compared the physicochemical properties of edible insect oils from silkworm (Bombyx mori) pupa (SP), sago palm weevil (Rhynchophorus ferrugineus) larva (PW), and bamboo caterpillar (Omphisa fuscidentalis; BC) to oils from chicken skin (CK), beef back fat (BF), pork back fat (PF), salmon belly (SB), sea bass belly (BB), coconut (C), and peanut (P). The fatty acid profiles and thermal behaviors (crystallization and melting) of the extracted oils were evaluated. PW and BC oils had more saturated fatty acids (SFAs) than CK, PF, SB, BB, and P oils. SP oil had equivalent SFA content to CK and BB oils. Insect oils exhibited similar monounsaturated fatty acid concentrations in all samples, except C oils. PW and BC oils exhibited a higher content of palmitoleic acid than the other oils. SP oils contained polyunsaturated fatty acids similar to those in SB and BB oils, which were higher than those in PW, BC, CK, BF, and PF oils. SP oil also exhibited the highest concentration of α-linolenic acid (C18:3 n-3). Arachidonic acid (0.01-0.02 g/100 g) in all insect oils was lower level compared to CK, BF, PF, SB, and BB oils. SP oil (0.03 g/100 g) exhibited a slightly higher level of eicosapentaenoic acid compared to PW (0.01 g/100 g) and BC (0.01 g/100 g) oils. The insect oils were liquid at ambient temperature, solid below -15°C, and required less energy (△Hm-max) for melting than other samples. This study indicated that insects, particularly SP, could serve as an alternative source of fat to meet its growing demand.

Applying Resistant Starch to Improve the Gel and Water Retention of Reduced-Fat Pork Batter.

Emulsified meat products contain high animal fat content, and excessive intake of animal fat is not good for health, so people are paying more and more attention to reduced-fat meat products. This study investigated the impact of varying proportions of pork back-fat and/or resistant starch on the proximate composition, water and fat retention, texture properties, color, and rheology characteristic of pork batter. The results found that replacing pork back-fat with resistant starch and ice water significantly decreased the total lipid and energy contents of cooked pork batter (p < 0.05) while improving emulsion stability, cooking yield, texture, and rheology properties. Additionally, when the pork back-fat replacement ratio was no more than 50%, there was a significant increase in emulsion stability, cooking yield, hardiness, springiness, cohesiveness, chewiness, and L* and G' values (p < 0.05). Furthermore, resistant starch and ice water enhanced myosin head and tail thermal stability and increased G' value at 80 °C. However, the initial relaxation times significantly decreased (p < 0.05) and the peak ratio of P21 significantly increased from 84.62% to 94.03%, suggesting reduced fluidity of water. In conclusion, it is feasible to use resistant starch and ice water as a substitute for pork back-fat in order to produce reduced-fat pork batter with favorable gel and rheology properties.

Enhancing quality control in emulsion-type sausage production: Predicting chemical composition of intact samples with near infrared spectroscopy

This research actively explores the potential of near infrared spectroscopy (NIR) for analyzing the chemical composition of emulsion-type sausages, focusing on critical factors like residual nitrite, moisture, protein, and fat content. To establish robust and generalizable models, we utilized a dataset of 100 experimentally prepared sausages encompassing a wide range of pork back fat replacement levels (5%, 15%, 30%, 45%, and 60%) and added sodium nitrite amounts (0, 80, 125, 250, and 375 ppm). An external validation set of 20 commercially sourced sausages further assessed the model’s real-world applicability. Partial least squares (PLS) regression calibration models with multiplicative scatter correction (MSC) pre-treatment demonstrated impressive accuracy for moisture (RMSECV = 0.57%, RPD = 9.8), fat (RMSECV = 1.17%, RPD = 9.5), and protein (RMSECV = 0.30%, RPD = 7.6). While residual nitrite prediction presented challenges due to its inherent complexity, the external validation yielded a competitive root mean square error of prediction (RMSEP) of 12.02 ppm, surpassing the average performance reported in similar studies (RMSEP ∼15 ppm) by 3 ppm. Importantly, sample homogenization did not significantly affect parameter prediction, highlighting the robustness of the NIR-based approach. These findings suggest that NIR spectroscopy, with its non-destructive, rapid, and cost-effective nature, could provide valuable tools for quality control and monitoring in the emulsion-type sausage industry. More importantly, improved nitrite prediction could pave the way for enhanced precision and control in sausage production, ultimately contributing to improved food safety and sustainability.

Production of polyunsaturated fatty acids in pork backfat fermented by Mucor circinelloides

Pork backfat (PB) contains excessive saturated fatty acids (SFAs), but lacks polyunsaturated fatty acids (PUFAs). Excessive SFAs can be used as a substrate for the growth of certain microorganisms that convert them into PUFAs and monounsaturated fatty acids (MUFAs), and the added value of PB can be enhanced. In this study, Mucor circinelloides CBS 277.49 and Lactiplantacillus plantarum CGMCC 24189 were co-cultured for conversion of PB into fermented pork backfat (FPB) with high level of PUFAs. Our results showed that the content of γ-linolenic acid (GLA) and linoleic acid (LA) in the surface of FPB reached 9.04 ± 0.14 mg/g and 107.31 ± 5.16 mg/g for 7-day fermentation, respectively. To convert the internal SFAs of PB, ultrasound combined with papain was used to promote the penetrative growth of M. circinelloides into the internal PB, and the GLA level in the third layer of fat reached 2.58 ± 0.31 mg/g FPB. The internal growth of M. circinelloides in PB was promoted by adjusting the oxygen rate and ventilation rate through the wind velocity sensor. When the oxygen rate is 2 m/s and the ventilation rate is 18 m3/h, the GLA level in the third layer of fat reached 4.13 ± 1.01 mg/g FPB. To further improve the level of PUFAs in PB, FPB was produced by M. circinelloides at 18 °C. The GLA content on the surface of FPB reached 15.73 ± 1.13 mg/g FPB, and the GLA yield in the second and third layers of fat reached 8.68 ± 1.77 mg/g FPB and 6.13 ± 1.28 mg/g FPB, the LA yield in the second and third layers of fat reached 105.45 ± 5.01 mg/g FPB and 98.46 ± 4.14 mg/g FPB, respectively. These results suggested that excessive SFAs in PB can be converted into PUFAs and provided a new technique for improving PUFAs in FPB.Key points• This article achieved the conversion of PUFAs in pork backfat by Mucor circinelloides CBS 277.49 and Lactiplantacillus plantarum CGMCC 24189.• This article solved the internal growth of M. circinelloides CBS277.49 in pork backfat by ultrasound combined with papain.• This article proposed an innovative of promoting the internal growth of M. circinelloides and increasing the PUFAs production by oxygen ventilation in pork backfat.

Bioaccessibility, Intestinal Absorption and Anti-Inflammatory Activity of Curcuminoids Incorporated in Avocado, Sunflower, and Linseed Beeswax Oleogels.

Beeswax oleogels (OGs), with a mechanical strength similar to pork backfat, were formulated with avocado (A), sunflower (S), and linseed (L) oils, applying a central composite design plus star point, and were evaluated as oral delivery vehicles of curcuminoids (OGACur, OGSCur, OGLCur). The incorporation of curcumin into the OG matrix significantly delayed both the formation of peroxides and conjugated trienes (K268 values), and the degradation rate of curcumin decreased with the increase of the oil polyunsaturated fatty acids (PUFA) content. The oil structuring did not affect the bioaccessibility of curcuminoids (>55% in all the OGs, regardless of the oil type), but it did reduce the release of fatty acids (~10%) during in vitro gastrointestinal digestion. The intestinal absorption, evaluated in Caco-2 cell monolayers, was higher for the micelle-solubilized curcumin from the digested OG than from unstructured oils, and it showed high anti-inflammatory potential by inhibiting the tumor necrosis factor-α (TNF-α) production compared to the positive control, both before and after the stimulation of ThP-1 cells with LPS. Regardless of the oil type, these beeswax-based OGs with gel-like behavior designed as fat replacers may be promising vehicles for the oral delivery of curcuminoids.

Red Dragon Fruit Peels: Effect of Two Species Ratio and Particle Size on Fibre Quality and Its Application in Reduced-Fat Alpaca-Based Sausages

This research aimed to assess the influence of red dragon fruit peels ratio (RDF-PR) from two species, Hylocereus hybridum (HH) and Hylocereus undatus (HU), and particle size (PS) on quality parameters of red dragon fruit peel powder (RDF-PP) and its further application in emulsified alpaca-based sausages as partial substitutes of pork-back fat. A three-level full factorial design (nine treatments) was employed to evaluate the effect of RDF-PR (HH(0%):HU(100%), HH(50%):HU(50%), and HH(100%):HU(0%)) and PS (499–297, 296–177, and &lt;177 µm) on the dependent variables: L*, a*, b*, C, h°, water-holding capacity, oil-holding capacity, swelling capacity, pectin yield, degree of esterification (analysed through FT-IR), and crude fibre content. The data analysed through a response surface methodology showed that treatment one (T1) is the best with the optimised conditions at 100% HU RDF-PR and PS of &lt;177 µm. The statistical validation of T1 exhibited the highest water-holding capacity (32.1 g/g peel), oil-holding capacity (2.20 g oil/g peel), and pectin yield (27.1%). A completely randomised design (four formulations) was then used to assess the effect of partial replacement of pork-back fat by T1 in emulsified alpaca-based sausages on the colourimetric, physicochemical, and texture properties (hardness, chewiness, cohesiveness, springiness, adhesiveness, and adhesive force). Likewise, a sensory hedonic scale was employed to evaluate the appearance, colour, odour, flavour, texture, and overall acceptability of sausages. The results revealed that 65.7% of pork-back fat content was successfully replaced compared with a control formulation. Additionally, F3 showed significantly (p &lt; 0.05) better colourimetric, physicochemical, and textural characteristics, such as lower hardness (34.8 N) and chewiness (21.7 N) and higher redness (a* = 19.3) and C (22.9), compared to a control formulation. This research presents RDF-PP as a promising fat substitute for developing healthier, reduced-fat meat products using fibre-rich agroindustry by-products.

Tailoring an egg white protein double network emulsion gel as a novel fat substitute for improving freeze-thaw stability of minced meat gel

This work aimed to investigate the effect of double network emulsion gel (DNEG) as a substitute for pork back fat (PBF) on the freeze-thaw stability of minced meat gel (MMG). We used slow acidification with glucono-δ-lactone (GDL) to induce egg white protein (EWP) gelation, while CaCO3 was affected by acidification to release Ca2+ to cross-link sodium alginate (SA), resulting in the gradual formation of DNEG networks. The MMG prepared using DNEG substitute for PBF had fine hardness and color after 5 freeze-thaw cycles (FTCs). This was due to the excellent plasticity of DNEG, which made it more capable of filling the voids of minced meat products, thus reducing the space for ice crystal growth. As a result, MMG prepared with DNEG could pass through the maximum ice crystal formation zone faster and avoid damage due to the formation of larger ice crystals. Notably, DNEG provided a barrier for the migration of immobilized water to bulk water in the sample. After several freeze-thaw treatments, the water holding capacity of samples prepared with DNEG decreased significantly slower, which slowed down the oxidation and decomposition of meat proteins. In summary, this study revealed the role of fat substitute DNEG as a favorable filling material for improving the freeze-thaw stability of minced meat products, and provided a technical reference for the development of healthy and storable meat products.

Rabbit meat in production of frankfurters

Meat is the most important source of protein, essential amino acids, minerals and B-complex vitamins in our diet. From the nutritional point of view, rabbit meat has a favourable composition due to its relatively low-fat content and belongs to dietary foods. At the same time, consumers of rabbit meat also recognize the typical delicate flavour and distinct tenderness of rabbit meat. For purpose to increase the consumption of rabbit meat products, we prepared and evaluated rabbit frankfurters with the addition of pork back fat and/or sunflower oil (rich in monounsaturated fatty acids, favourable ratio of n-3/ n-6 fatty acids) and without the addition of phosphate. The results showed that the frankfurters with pork back fat are more acceptable from a sensory point of view. The absence of phosphate results in a lighter, less rubbery texture, and at the same time the oxidation process is faster. It can be concluded that rabbit meat is suitable for processing into meat products, especially when combined with vegetable oils, which gives good results, as such a product approaches the optimal fatty acid composition and thus has a positive effect on the health of the consumer.

Modulating the textural and structural characteristics of curdlan-potato protein gel with different concentrations

The use of protein-polysaccharide complexes as fat substitutes to reduce fat and maintain fat functionality has received much attention in the food industry. In the present study, complex gels were developed using curdlan (CR) and potato protein (PP) for the potential of development as a solid fat analog. The effect of PP concentrations (1–6%) on the texture, water-holding capacity, freeze-thaw stability, and microstructure of the resultant curdlan-potato protein (CP) gel was investigated. The results showed that the texture properties of the gel with 3% PP were the most similar to those of pork backfat. The addition of PP contents had a pronounced enhancement in the water-holding capacity, and freeze-thaw stability tended to increase and subsequently decrease after 4% PP (p < 0.05). The low-field NMR confirmed the addition of PP improved the migration and tighter binding of water. The elastic modulus of CP gels increased initially before declining, reaching a maximum at a protein concentration of 3% and a minimum at 5%. The microstructure demonstrated a gradual aggregation and phase separation process of PP and CR, resulting in a strengthened skeleton network. These findings provide a reference for the development of a novel non-animal fat analog in the food industry.

Efficacy of Chitosan, Pectin and Xanthan as Cold Gelling Agents in Emulsion Gels Stabilized with Legume Proteins to Be Used as Pork Backfat Replacers in Beef Burgers.

This study aimed to develop stable emulsion gels enriched in polyunsaturated fatty acids, formulated with a mixture of olive (75%) and linseed (25%) oils, by incorporating two different stabilizers-pea and soy protein isolates-and three different cold gelling agents-chitosan, pectin and xanthan-to be used as pork backfat replacers in beef burgers. The color, pH, stability and textural properties of the emulsion gels were analyzed as affected by cold storage (4 °C, 7 days). Proximate composition, fatty acid content, technological and sensory properties were determined after burger processing. Meanwhile, color, pH, textural parameters and lipid oxidation were monitored in burgers at 0, 5 and 10 days of storage at 4 °C. A reduction of the fat content between 21.49% and 39.26% was achieved in the reformulated burgers as compared with the control, while the n-6/n-3 polyunsaturated fatty acid ratio decreased from 5.11 to 0.62. The highest moisture and fat retention were found in reformulated burgers made with xanthan, both with pea and soy proteins; however, their textural properties were negatively affected. The reformulated burgers made with chitosan were rated highest for sensory attributes and overall acceptability, not significantly different from the controls.

Evaluation of bigel systems as potential substitutes to partially replace pork backfat in semi-dry sausages

Bigels prepared with olive oil oleogels admixed with κ-carrageenan or κ-carrageenan and gelatin hydrogels (BG1 and BG2, respectively) were characterized with respect to microstructure and textural properties and were used as pork backfat alternatives in semi-dry sausages. Stable oleogel-in-hydrogel type bigels were formed, with BG2 having higher hardness values. Control sausages (CF) were formulated with 20% pork backfat and sausage treatments B1F and B2F had 50% of the pork backfat substituted by BG1 and BG2 bigels, respectively. Moisture, water activity, texture, microbial counts, sensorial and nutritional attributes of the resulting sausages were assessed during fermentation and after pasteurization and storage. Substituted sausages had increased weight loss, moisture, and water activity. Color evaluation revealed that the treatments with bigels exhibited the same trend in color formation and no differences were recorded in L* and a* values of the sausages. Total viable counts and lactic acid bacteria populations were not affected by the addition of bigel systems. Regarding the texture parameters, B2F semi-dry sausages exhibited similar values of hardness and cohesiveness to CF. Sausages formulated with bigels exhibited a reduction in energy (20%), fat (27%), saturated fatty acids (30%) and cholesterol (∼6%) content. B2F sausages had similar liking scores with CF, and they did not show any undesirable sensory attributes. The results demonstrate that bigels are a promising fat alternative to manufacture semi-dry meat products with lower fat content and a better nutritional profile.

Peculiarities of the use of mushrooms Agaricus bisporus and Pleurotus os-treatus and effect on the quality and microstructure of chicken batter

The low energy density and healthier ingredients can replace some or all of the fat in emulsified meat products according to some studies. Meat products with low fat content are the major trends in developing healthier meat products. Attention is drawn that the animal fat is essential for emulsified meat products to enhance the stability, flavor, cooking yield, and texture. As can be noted that compound fat substitutes, such as vegetable protein and carrageenan, vegetable oil and vegetable fibre, cellulose, and water, improved texture better than single fat substitutes. Nevertheless, the character of the emulsified meat product will eventually decrease as the amount of animal fat is reduced. The combination of Agaricus bisporus and Pleurotus ostreatus mushrooms was employed as a fat substitute to replace the pork-back fat in chicken batters in order to create low-fat chicken products. The microstructure, color, texture, and water holding capability of chicken batters were examined. According to the findings, the combination of Agaricus bisporus and Pleurotus ostreatus enhanced the cooking yield, texture, water holding capacity, redness, and yellowness of chicken batters, while lowering their brightness. The greatest quality and a compact, uniform, and continuous microstructure were seen in the chicken batters when Agaricus bisporus and Pleurotus ostreatus at a ratio of 1:1 substituted 40 % pork-back fat. To summarize, Agaricus bisporus and Pleurotus ostreatus are a viable fat alternative in the production of low-fat chicken products.

PSV-3 Evaluation of Functional Properties of Chitin as a Novel Non-Meat Ingredient for Manufacturing Meat Emulsions

Abstract There has been a growing demand for developing naturally occurring non-meat ingredients, which could replace or reduce synthetic additives that have been used extensively in manufacturing processed meat products. Chitin, which is a major component of crustacean shells and a precursor of chitosan, is the second most abundant polysaccharide followed by cellulose. While some functional properties of chitin such as water/fat binding capacity and emulsifying ability have been reported, there are almost little to no studies determining the efficacy of chitin as a non-meat ingredient. The objective of this study was to evaluate the functional properties of chitins as a novel non-meat ingredient for manufacturing emulsified sausages. Fresh pork hind leg and pork back fat from 9 carcasses at 4 d postmortem were obtained. The trimmed pork hind legs were randomly assigned into three independent batches for emulsion and sausage production. Chitins were extracted from chitin-like material (CLM) from two different crustacean shell sources, such as crawfish (CF) and blue crab (BC). The CLM were extracted and used in the following order: drying-deproteinization (1% calcium oxide)-demineralization (2N HCl)-deacetylation (6M NaOH). For the emulsion and sausage manufacturing, control was prepared with 70% raw meat, 18% backfat, 10% water, 1.5% sodium chloride and 0.5% sodium phosphate. The treatments were prepared with 70% raw meat, 18% backfat, 10% water, 1.5% sodium chloride, 0.2% phosphate, and 0.5% CLM. The functional properties of CLM were evaluated by measuring binding capacity, emulsion capacity, and color attributes. For the evaluation of meat batter and emulsified sausage products, instrumental color, texture profile analysis, emulsion stability, cooking loss, and pH were measured. The data analysis was conducted by mixed model, and least squares means for all traits were separated (F-test, P &amp;lt; 0.05) by using least significant differences. No significant difference in the color attributes of meat batter between the control and the treatments (CF and BC) was found. The chitin added products from both CF and BC had a greater emulsion stability, where the treatment showed about two time greater in emulsion stability compared with control (P &amp;lt; 0.05). In particular, chitin extracted from CF showed greater water and fat binding capacity than CB (P &amp;lt; 0.05). In the emulsified sausage, the treatments showed significantly greater redness and hardness than control (P &amp;lt; 0.05). In particular, the CF showed greater cohesiveness and gumminess than control or BC (P &amp;lt; 0.05). The results of the present study showed the beneficial impacts of chitin addition for meat emulsions. The chitin inclusion improved the quality characteristics of emulsified sausage such as textural properties and emulsion stability, while less amount of phosphate was added compared with control. Additionally, chitin from crawfish had superior functional properties to that from blue crab, indicating different chitin functionality between crustacean shell sources.