The influence of oil-wet calcite cements on oil migration in clastic reservoirs is of significant interest in the field of petroleum exploration. However, the microscopic migration of oil in microscopic pore spaces with oil-wet calcite cements has not been directly observed. Microscopic observations of oil migration were conducted in a glass micromodel at normal temperature and pressure to investigate the influence mechanism of oil-wet calcite cements on oil migration in pore spaces. The calcites were precipitated as cements prior to the oil migration. A two-dimensional glass micromodel with two interconnected spaces with the same pore throat structures was used in the experimental study. A new method to create firmly adhering calcites was used to precipitate equal amounts of calcites in the two interconnected spaces. The oil migration was recorded in real-time under a microscope throughout the experiment. The experimental results show that oil migration occurs faster in spaces with than without oil-wet calcites. During the oil migration in the pore-throat spaces, the oil migrates towards the surfaces of the oil-wet calcites when the oil is in contact with the calcites. The presence of oil-wet calcites significantly reduces the oil breakthrough pressure. The electrostatic attraction between the carboxylic groups and calcites and the intermolecular forces between the carboxylic groups and organic molecules in the oil cause the oil migration towards the surfaces of the oil-wet calcites. The experimental results indicate that oil preferentially migrates into clastic reservoirs that possess appropriate amounts of oil-wet calcite cements precipitated prior to oil emplacement.