A149 Aims: The number of cases of islet transplantation has increased over the past years following the development of the Edmonton Protocol. The shortage of donor is one of the problems to still be solved. Xenotransplantation of porcine islets could provide an unlimited source of insulin-producing cells. Since it is necessary to check the quality control including microbiological test of porcine islets prior to transplantation, culture is required. However, it is difficult to culture them because of their fragileness. We examined the effect of adding polyphenol for culture of porcine islets. Methods: Islets were isolated from over two years old pigs (n=5). They were cultured in Media 199 with 10% FCS supplemented with polyphenol at concentrations of 0 (control), 30, 60, 120 ug/ml and incubated at 37 °c. We compared the results of culture on culture recovery rate (islet yield after culture / islet yield before culture), viability and morphology after 1, 7, 14, 28 day of culture. Functionality with glucose stimulation test was assessed at day 1. Results: Culture recovery rates were day1: 52.2+/-21.7%, 65.9+/-24.8%, 87.9+/-22.2%, 93.3+/-41.3%, day7: 13.2+/-1.0%, 20.0+/-7.8%, 39.6+/-9.5%, 60.7+/-16.2% (p<0.05 vs control, respectively), day14: 1.6+/-1.2%, 9.7+/-4.0%, 14.2+/-5.6%, 22.0+/-2.6% (p<0.05 vs control, respectively), day28: 0.5+/-0.3%, 4.1+/-3.7%, 10.0+/-6.1%, 15.4+/-2.5% in groups 0, 30, 60, 120 ug/ml of polyphenol, respectively. Functionality indicated by stimulation index was 1.3+/-0.18, 3.2+/-3.1, 7.3+/-2.9, 10.74+/-5.3 (p<0.05 vs control), respectively. However, no significant differences were noted in islet viability and morphology at each day with or without polyphenol. Conclusions: Polyphenol increases culture recovery rates and functionality of islets from porcine pancreata by dose dependent. It is thought that this will be applicable to islet xenotransplantation.