Parkinson’s is the second most common progressive neurodegenerative disease and affects 1-2% people over the age 50. Levodopa is the drug of choice in the treatment of Parkinson’s disease and exhibits low oral bioavailability (30%) and very low brain uptake. In an attempt to improve brain uptake and to avoid degradation of levodopa in peripheral circulation, brain targeting of levodopa loaded pharmacosomes via nasal route has been investigated. Pharmacosomes are colloidal dispersions of drug covalently bound to lipids and may exist as ultrafine vesicular, micellar or hexagonal aggregates, depending on the chemical structure of the drug-lipid complex. Pharmacosomes loaded with levodopa were prepared by solvent evaporation method and the optimized formulation contained levodopa and egg lecithin in the ratio of 1:3. The mean globule size, PDI, zeta potential, drug content, entrapment efficiency and drug release of formulation (F5) were 123.2nm, 0.211, -29.1mV, 96%, 99.97% and 62.1% respectively. Permeation enhancer, chitosan was incorporated at 0.5% concentration to optimal formulation (F7). The mean globule size, PDI, zeta potential, drug content, entrapment efficiency and drug release of optimised formulation (F7) were 125.5nm, 0.115, +33.7mV, 96.5%, 99.98% and 91.2% respectively. Formation of covalent bond between levodopa and lecithin was confirmed by FTIR spectra showing peak at 1639.9cm-1. Ex-vivo permeation studies using Franz diffusion cell on porcine nasal mucosa showed flux of 96.61 μg/cm2/h and 302μg/cm2/h. The steady state flux of F5 and F7 formulations was significantly high (P Key words: Brain targeting, Levodopa, Nasal delivery, Parkinson’s disease, Pharmacosomes.