Population Status Research Articles

Ankle brachial index,a non invasive tool predicts cardiovascular events after acute myocardial infarction:a 3 year follow -up study

Abstract Background / Introduction The ankle-brachial index (ABI) is a non-invasive tool that was initially used for the diagnosis of lower-extremity peripheral artery disease (PAD). It has been proven that ABI is a marker of atherosclerosis in other vascular sites and can therefore be used to predict cardiovascular events regardless the presence or not of PAD symptoms. Purpose This study aims to examine the prognostic value of ABI in the setting of acute myocardial infarction (MI), as up till now there is not enough relevant evidence. Method We followed up 441 patients [79% male;mean age 62 years;67% hypertnesive; 30% with diabetes mellitus (DM); 52% current smokers] who were hospitalized because of MI for a period of three years. All patients underwent baseline estimation of clinical and laboratory parameters during theri hospitalization.The ABI was measured according to established methodology using a certified automated device and abnormal ABI defined as a value ≤ 0.9. The primary endpoint was a composite of all-cause and cardiovascular death, as well as major cardiovascular events including decompensated heart failure, new acute coronary syndrome, cerebrovascular event, malignant arrhythmia, PAD event and new onset renal dysfunction. All events were assessed both in-hospital and within 3 years of follow-up. Results Our study population had a mean ABI of 1.1 (IQR: 1.00 to 1.18). With the use of reduced multivariate regression models ABI was proven to be a prognostic indicator for the in-hospital composite endpoint. Patients with an abnormal ABI had a threefold risk of in-hospital events [HR 2.93, 95% CI: 1.48-5.81, p=0.002]. After conducting multivariate analysis ABI maintained its predictive power of all-cause mortality [HR 2.88, 95% CI: 1.53 – 5.42, p=0.001], regardless of hypertension DM, LDL levels and smoking status of the study population in 3 years follow-up. Conclusion An impaired ABI is associated with greater risk of in-hospital events in patients with acute MI. Moreover, ABI emerges as an independent predictor of long-term all-cause mortality in those patients.The findings of this study suggest that subclinical and clinical PAD are associated with poor outcomes among patients with acute MI; thus, routine ABI tests enhance risk stratification and might carry important prognostic significance in these patients regardless of PAD symptoms.

Cardiovascular Risk Scores and Migraine Status

A previous cohort study in the US found that women with higher cardiovascular risk were more likely to have a history of migraine but less likely to have active migraine. Extrapolating these results to men and European individuals is crucial to understanding the complex association between migraine activity status and vascular health in other populations. To evaluate the association pattern between a cardiovascular risk score, the most recent European version of the Systematic Coronary Risk Evaluation 2 (SCORE2) risk estimation system, and migraine activity status in Dutch men and women. The prospective population-based Lifelines cohort consists of community-dwelling adults residing in the northern part of the Netherlands. Individuals with a terminal illness, incapacitated individuals, including those with a severe mental illness, or who were unable to visit their general practitioner or complete the questionnaires were excluded from participation within Lifelines. Participants whose data on the cardiovascular risk scores and migraine status were complete were included in the analysis. Data on baseline characteristics were collected between November 1, 2006, to December 31, 2014. Cross-sectional and follow-up analyses were conducted within the prospective cohort. Questionnaires were sent approximately every 1.5 to 2.5 years, and the last self-reported migraine assessment took place between October 1, 2019, and January 31, 2021. Data were analyzed from March 1, 2022, to August 16, 2024. The SCORE2 is a sex-specific European cardiovascular risk score that includes age, cholesterol levels, smoking status, diabetes, and systolic blood pressure. The primary outcome was the association pattern between cardiovascular risk scores and migraine activity status. SCORE2 risk scores were measured once at baseline; groups of the SCORE2 (<1.0%, 1.0% to <2.5%, 2.5% to <5.0%, 5.0% to <7.5%, 7.5% to <10.0%, and ≥10.0%) were created based on the sum of points of individual risk factors. Migraine activity status was assessed using self-reported questionnaires and classified as (1) prevalent (ie, migraine at baseline), (2) incident (ie, no migraine at baseline but migraine in ≥1 follow-up), and (3) none. To evaluate the influence of age, we conducted stratified analyses of the SCORE2 by age categories (<40, 40-49, and ≥50 years). The total study population consisted of 140 915 individuals at baseline with a mean (SD) age of 44.4 (12.7) years, of whom 58.5% were women. In total, 25 915 individuals (18.4% of the total population) had prevalent migraine and 2224 (1.9% of the 115 000 without prevalent migraine) had incident migraine. The odds of having prevalent and incident migraine, compared with individuals with a SCORE2 category of less than 1.0%, varied and decreased with increasing SCORE2 categories, with odds ratios (ORs) for prevalent migraine ranging from 0.93 (95% CI, 0.90-0.96) for a SCORE2 category of 1.0% to less than 2.5% to 0.43 (95% CI, 0.39-0.48) for a SCORE2 category of at least 10.0% and, for incident migraine, from 0.63 (95% CI, 0.57-0.69) for a SCORE2 category of 1.0% to less than 2.5% to 0.17 (95% CI, 0.10-0.27) for a SCORE2 category of at least 10.0%. A similar pattern was observed in both sexes but more profound in women. In women, ORs for prevalent migraine ranged from 1.21 (95% CI, 1.16-1.25) to 0.70 (95% CI, 0.58-0.83) (vs 1.19 [95% CI, 1.09-1.29] to 0.84 [95% CI, 0.72-0.99] in men) and, for incident migraine, 0.72 (95% CI, 0.64-0.80) to 0.20 (95% CI, 0.07-0.43) (vs 1.18 [95% CI, 0.92-1.52] to 0.44 [95% CI, 0.22-0.78] in men). Models with incident migraine as the outcome showed lower ORs across the ascending cardiovascular risk score categories. Age stratification suggested that the association between cardiovascular risk scores and migraine activity status were unlikely to be strongly influenced by age. In this cohort study of community-dwelling Dutch adults, the odds of having prevalent or incident migraine decreased with increasing cardiovascular risk score categories. These results support the hypothesis that a relatively healthy cardiovascular system increases the probability of having active or developing migraine in the future, especially among women. Sex differences might play a pathophysiological role in the association between migraine activity and vascular health.

A Review of Science and Conservation Management for the Cumberland Sound Beluga Population

For many centuries, belugas, or white whales (Delphinapterus leucas), have been a major source of subsistence and cultural identity for the Inuit living along the shores of Cumberland Sound, southeastern Baffin Island. During the late 1800s and first half of the twentieth century, the whales were also heavily exploited commercially for their oil and skins. By the late 1960s and early 1970s it had become clear that the beluga population was greatly reduced from its historical abundance, and efforts began to limit the harvest and monitor the population. The purposes of this paper are to (i) provide a synthesis of developments in Cumberland Sound beluga science and harvest management since 1980 and (ii) describe and discuss efforts to improve the conservation status of the beluga population. Despite large investments in research since the transition to co-management under the Nunavut Agreement, much uncertainty and disagreement remains. Best scientific estimates of current beluga numbers are in the range of 1000 – 1500, with no clear evidence of an increasing or decreasing trend. Officially reported annual landings of harvested whales for the Baffin Island community of Pangnirtung in recent years have ranged between 15 (1993) and 52 (2006), with an average of around 40 whales. Ongoing known or potential threats identified by hunters and scientists include overharvest, ecosystem (including climate-driven) change, interactions with commercial fisheries, predation by killer whales, and stress due to vessel noise. Addressing these issues will require continued research and improved relations between Inuit and the government. Fresh approaches are needed. Newly available analytical and procedural tools may help to overcome longstanding issues that are deeply embedded in cultural and philosophical differences.

Cultural adaptation, validity and reliability of the Turkish version of north star ambulatory assessment.

The North Star Ambulatory Assessment (NSAA) is a functional motor outcome measure originally developed for patients with Duchenne muscular dystrophy (DMD). The aim of this study was to perform the cultural adaptation and investigate the validity and reliability of the Turkish version of the NSAA (T-NSAA) in DMD. After translation process, internal consistency, interrater and test-retest reliability of the NSAA were determined by using the Cronbach Alpha Coefficient and Intraclass Correlation Coefficient (ICC), respectively. Absolute reliability was determined by using the Standard Error of Measurement (SEM) with minimal detectable change at 95% limits of confidence (MDC95). Lower limb functionality of children was evaluated by Vignos Lower Extremity Scale (VLERS). To establish convergent validity, the correlations between T-NSAA and Motor Function Measure (MFM-32), 6-minute walk test (6-MWT), and VLERS were analyzed by using the Spearman's correlation coefficient. The study was completed with 86 patients with DMD whose age were mean 104.56 ± 24.66 months. The internal consistency (Cronbach's α = 0.94), intra-reliability (ICC = 0.977) and inter-reliability (ICC = 0.972) of T-NSAA were excellent. SEM and MDC values were low indicating satisfactory absolute agreement (< %10). The T-NSAA had strong correlations with the MFM-total score, 6-MWT, and VLERS (p < 0.01). T-NSAA is a valid and reliable tool to assess ambulatory status of Turkish-speaking DMD population. NCT05549999, Date of registration: September 19, 2022.

Dietary pattern transition and its nutrient intakes and diet quality among Japanese population: results from the 2003-2019 National Survey.

While many Asian countries undergo dietary transitions, little is known about Japan's dietary pattern changes and their impact on nutritional intake. We aimed to examine 17-year trends in dietary patterns and nutrient intakes in Japan. Principal component analysis was used to derive dietary patterns. The nutrient intake of subjects with principal component scores in the highest quartiles of each dietary pattern was assessed using the NRF9.3 which is a scale that evaluates the entire diet in terms of nutrient density. Japan. We used data from the National Survey 2003-2019 (67,066 women, 55,133 men). Two common dietary patterns were identified in both sexes: the 'Japanese style' and the 'bread and dairy' pattern. Additionally, two other patterns: 'meat and oil' and 'noodles', were pronounced in men. Over the 17 years, the scores of the 'Japanese style' pattern decreased while the 'meat and oil' and the 'bread and dairy' patterns increased. The nutrition assessment result showed that the highest quartiles of the 'Japanese style' pattern had higher NRF scores (women: 716.0, men: 670.5) (p<0.001), whereas those of the 'bread and dairy' pattern had a lower score (636.9 in women, 661.2 in men) (p<0.001). Trend analysis in this study suggests the ongoing Westernization of diet in Japan. A decreasing trend of the dietary pattern with the most preferable nutrient profile (i.e., the 'Japanese style') might indicate a potential decrease in beneficial nutrient intake and, thus, a deterioration of the nutritional status of the Japanese population.

Recent data of the biology of the twaite shad, Alosa fallax returning for spawning to the Szczecin Lagoon (southern Baltic Sea)

In 2022–2023 specimens of A. fallax are recorded for the first time in approximately 17 years in Szczecin Lagoon. We collected 11 specimens of juveniles, 9 premature and mature females, and 9 premature and mature males (20.3–40.7 cm of total length, aged from 1+ to 4+). The 2+ aged fish was slightly dominated (34.5% of the whole sample). Females in the Szczecin Lagoon were ready to spawn in the age of 3+ and 4+ years, while males partly in 2+, and in the age of 3+ and 4+. Fulton’s condition factor of this fish was 0.93±0.14 and Clark’s 0.78±0.14. Analysis of correlations between the total length and individual weight of fish revealed that the growth of A. fallax was allometric (b &amp;lt; 3.0). The range of gonadosomatic index (GSI) was 0.12–26.59%, while the mean of the absolute and relative fecundity was 58,756 eggs per female and 139,650 eggs per kilogram of body weight. The obtained results indicate the occurrence of an anadromous population of Alosa fallax in the studied water areas. However, there is still a need to obtain detailed information on the population status and the biology of A. fallax in these areas in order to designate special conservation areas (SACs) for their protection, especially taking into account spawning habitats.

Evaluating vitamin D status in Chinese pre-school children using dried blood spots coupled with liquid chromatograph coupled with mass spectrometer.

Vitamin D is an essential micronutrient for multiple physiological processes, and its deficiency remains a world-wide public health problem that cannot be ignored. Dried blood spot (DBS) is a convenient tool in large-scale epidemiological studies, but its application in evaluating vitamin D status in Chinese population is still scarce. Herein, we aimed to determine the vitamin D status in Chinese pre-school children using DBS coupled with LC-MS/MS method. We first developed a sensitive and reliable method for the determination of 25-hydroxyvitamin D (25(OH)D) in DBS samples using an ultra-high-performance liquid chromatograph coupled with triple quadrupole mass spectrometer (UHPLC-QQQ-MS/MS). Next, we conducted a pilot study to compare the 25(OH)D concentration in DBS and serum samples. Finally, the assay method was used to evaluate vitamin D status in Chinese pre-school children. The present method was validated to be reliable and robust for the determination of 25(OH)D in DBS samples. Comparable consistency was observed between the 25(OH)D concentration in DBS and serum samples. A total of 3826 DBS samples collected from children aged 1-7 years were determined. The median concentration of 25(OH)D was 19.57 ng/mL (interquartile range 14.73-24.36 ng/mL), and decreased from 1 to 7 years of age. In addition, 13.51% of male children and 15.12% female children are found to be deficient in 25(OH)D. DBS coupled with LC-MS/MS is a feasible strategy to evaluate vitamin D status in epidemiological studies. And vitamin D deficiency remains a common health problem in Chinese pre-school children.

Propagating observation errors to enable scalable and rigorous enumeration of plant population abundance with aerial imagery

Abstract Estimating and monitoring plant population size is fundamental for ecological research, as well as conservation and restoration programs. High‐resolution imagery has potential to facilitate such estimation and monitoring. However, remotely sensed estimates typically have higher uncertainty than field measurements, risking biased inference on population status. We present a model that accounts for false negative (missed plants) and false positive (misclassified or double‐counted plants) error in counts from high‐resolution imagery via integration with ground data. We apply it to estimate the abundance of a foundational shrub species in post‐wildfire landscapes in the western United States. In these landscapes, plant recruitment is crucial for ecological recovery but locally patchy, motivating the use of spatially extensive measurements from unoccupied aerial systems (UAS). Integrating &gt;16 ha of UAS imagery with &gt;700 georeferenced field plots, we fit our model to generate insights into the prevalence and drivers of observation errors associated with classification algorithms used to distinguish individual plants, relationships between abundance and landscape context, and to generate spatially explicit maps of shrub abundance. Raw counts of plant abundance in high‐resolution imagery resulted in substantial false negative and false positive observation errors. The probability of detecting (p) adult plants (0.25 m tall) varied between sites within 0.52 &lt; &lt; 0.82, whereas the detection of smaller plants (&lt;0.25 m) was lower, 0.03 &lt; &lt; 0.3. On average, we estimate that 19% of all detected plants were false positive errors, which varied spatially in relation to topographic predictors. Abundance declined toward the interior of previous wildfires and was positively associated with terrain roughness. Our study demonstrates that integrated models accounting for imperfect detection improve estimates of plant population abundance derived from inherently imperfect UAS imagery. We believe such models will further improve inference on plant population dynamics—relevant to restoration, wildlife habitat and related objectives—and echo previous calls for remote sensing applications to better differentiate between ecological and observational processes.

Germplasm Resources Evaluation of Cultured Largemouth Bass (Micropterus salmoides) in China Based on Whole Genome Resequencing.

Background: Largemouth bass (Micropterus salmoides), a valuable freshwater fish species, has experienced significant genetic decline in China due to prolonged domestic breeding and limited introduction of new genetic material. It is necessary to have a comprehensive understanding of the genetic status of largemouth bass populations in China. Method: In this study, we conducted population genetic analyses on nine cultured largemouth bass populations using whole genome resequencing. Results: A total of 3.23 Tb of clean bases were generated, with average Q20 and Q30 values of 98.17% and 94.25%, respectively, and 2,140,534 high-quality SNPs were obtained. Relatively high genetic diversity was observed across all populations. Combined with linkage disequilibrium (LD) patterns, the Wanlu (WL) population possessed the highest genetic diversity, and the Longyou (LY) population possessed the lowest genetic diversity. Additionally, population structure analyses, including pairwise F-statistics, phylogenetic trees, PCA, and admixture analysis, revealed significant genetic differentiation, particularly between the WL, LY, and other 7 populations, while also indicating the occurrence of a common admixture event. Finally, TreeMix inferred migration events from the WL to the Chuanlu (CL) population and from the Taiwan breeding population (TWL) to the Guanglu (GL) population. Conclusions: These findings provide a critical foundation for developing conservation and breeding strategies for largemouth bass in China.

Testing best practices in small scale fisheries management: Evidence from a collaborative intervention in two marine protected areas of the central Mediterranean Sea

Adopting best practices (BPs) in small scale fisheries (SSF) is generally acknowledged to enhance ecological and social conditions, improving SSF management overall. Yet studies that empirically assess the effects of BPs on social-ecological outcomes (e.g., fishers' catches, revenues, and level of support towards management measures) remain limited. This study aimed to assess the effects of adopting BPs on catch per unit of effort (CPUE), revenue per unit of effort (RPUE) and small-scale fishers' perceptions in two Marine Protected Areas (MPAs) in Campania, Italy (Santa Maria di Castellabate (CAS-MPA) and Regno di Nettuno (NET-MPA)). Two BPs were implemented and tested in the two MPAs: i) five selective gears with three different mesh/hook sizes and ii) a participatory approach to increase dialogue between fishers and managers. The results from 36 surveys with small scale fishers and 158 SSF operations monitored using photo-sampling and image analysis techniques (between 2020 and 2022) are presented. Analyses of landings showed that significant differences in CPUE and RPUE between mesh/hook sizes were found in a limited number of cases (i.e., combination of fishing gears used in the 2 MPAs). Significant but divergent effects were found on CPUE in the two MPAs for gillnet used to target amberjack (GNA) only. RPUE was significantly higher in the NET-MPA when adopting the largest mesh size for GNA and the largest hook size for bottom longlines (BLL). When analyzing perceptions, fishers generally reported that the BPs adopted had a positive impact on their revenues, improved their relationship with MPA managers and increased their support for the MPA. In addition, eight stakeholder focus groups were held which helped investigators better understand the context, feedback the data gathered from the research and open up dialogue between the relevant stakeholders. The study concluded that increasing mesh/hook sizes, within the range tested here, does not negatively impact fishers’ revenues, while likely improving the status of target populations. Moreover, increasing dialogue between fishers and decision-makers can generate more positive perceptions towards the MPA and improve mutual understanding, helping to reconcile biodiversity conservation with human wellbeing for SSF.

New insights into the geographical distribution of brown bears Ursus arctos in Nepal

Abstract In the current IUCN Red List assessment, the south-western distribution range of the brown bear Ursus arctos in Nepal ends in Upper Mustang, in the central Himalaya, and extends northwards into the Tibetan Autonomous Region of China. Although brown bears have been recorded further west of Upper Mustang, details of these findings have not been published previously. Using camera traps, we present new evidence of brown bears in Limi Valley, Upper Humla, north-western Nepal. Covering a study area of 336 km2, we deployed 61 camera traps for 3,145 trap-nights during July–October 2021 and 10,748 trap-nights during June 2022–October 2023. In 2021 we recorded a single independent image of a brown bear, followed by 23 independent images during 2022–2023. The images were captured during spring and autumn but not in summer and winter. These new records increase the distribution range of the brown bear in Nepal, extending its global range south-westwards. Our results indicate the significance of Limi Valley as a stronghold for brown bears in Nepal and underscore the importance of formally protecting the currently unprotected wildlife habitats in Limi Valley. Our study emphasizes the need for further research into the ecology and population status of brown bears in Nepal.

Cross-sectional study for profiling health status, health-related demographics, and health-seeking behaviour of the Scheduled Caste Population in selected areas of Chennai and Chengalpet Districts of Tamil Nadu

Background: Scheduled Caste (SC) population forms a large section of the Indian population who are often marginalised and deprived of social, economic and health benefits due to ignorance and socio-cultural milieu. Though various studies have been conducted on the socio-economic and political conditions of the SC population in Tamil Nadu, limited data is available on the status and determinants of their health. Objectives: The study's objectives were to estimate the health status of the SC population, health-related socio-demographic conditions and the population's health-seeking behaviour. Method: The observational, cross-sectional study was conducted in five SC-predominant areas of Chennai and Chengalpet districts of Tamil Nadu. Their health status, socio- demographic conditions, including household conditions, and health-seeking behaviour were estimated. Result: A total of 1452 households and 6072 participants of the SC community were studied, mostly belonging to the Adidravida community. Most households were of Upper-lower socioeconomic class with basic household amenities. A total of 11.54% of the adult population had a history of diagnosed chronic illnesses. Nearly one-fifth of reproductive-age women had menstrual irregularities. Non-communicable and infectious diseases were the most frequent reason for health care centre visits. Conclusion: Extensive and continuous screening of health status and chronic diseases is essential for early diagnosis and intervention. Focused interventions on improving socio- demographic status can further strengthen the health condition of the SC community. Integrating all systems of medicine in public health can help cater to the health requirements of all sections of the community. Concerted efforts can cut down the burden of chronic diseases.

Intra and interspecies discrimination of three teleost species collected from the Komo Estuary in Gabon in relation to living depth levels using otolith shape analysis

ABSTRACT This study aimed to examine otolith shape and fluctuating asymmetry (FA) in pelagic Ethmalosa fimbriata, demersal Galeoides decadactylus, and benthic Cynoglossus monodi and assess their population status to inform on management procedures and verify whether or not otolith shape differs with living depth levels of these species in Lebe fishing site located along Komo Estuary, central-western Gabon. At intraspecies level, otolith shape analysis showed a bilateral asymmetry only in left and right otoliths between males and females of E. fimbriata and C. monodi. DFA separated between left and right and left-left and right-right otoliths shape of males and females within each species. At interspecies level, a significant bilateral asymmetry in left and right otolith shape was found among males and females of each species. DFA completely separated the left and right otoliths of both males and females of three species and confirmed the presence of three separate populations representing the three species, which should be managed separately. A significant FA on right and left otolith shapes was found among males and females at intra and interspecies levels. Accordingly, results indicated a close relationship between asymmetry of otolith shape and pelagic and benthic life characteristics of E. fimbriata and C. monodi.

Investigation of the risk factors associated with prediabetes in normal-weight Qatari adults: a cross-sectional study

Type 2 diabetes is one of the most prevalent chronic diseases in the world, and more people than ever before have impaired glucose tolereance, or prediabetes. Many patients with impaired glucose tolerance and undiagnosed diabetes do not know that their glucose metabolism system has been in a state of disorder. Every year, about 5-10% of prediabetics develop diabetes. One of the important achieving factors may be the increase in blood lipids. However, it is not clear whether triglyceride is associated with impaired glucose tolerance and prediabetes in the Qatari population. Therefore, we investigated the relationship between the first several clinical variables and prediabetes status in normal and overweight populations. We conducted a cross-sectional study using data from the Qatar Biobank program. The study included 5,996 participants who were adults over the age of 20. We collected information about participants’ fasting blood glucose levels with other clinical measurements and used various machine learning models and logistic regression to study the association between the clinical measurements and prediabetes for normal and overobese weight groups. The use of several machine learning models showed that, after adjusting the potential confounding factors such as age and sex, Triglyceride has been demonstrated to be positively correlated with prediabetes, and there was a special population dependence phenomenon. Among them, nonobese people (p < 0.05). The effect value and 95% confidence interval and OR of triglyceride on prediabetes was 2.79 and (e0.78, e1.28), respectively.

Peripheral Population Status and Habitat Suitability Assessment of the Kiang (Equus kiang) on the Eastern Tibetan Plateau

Shiqu County, Sichuan Province, forms the eastern edge of the distribution area of the kiang (Equus kiang). It is important to assess the population and habitat status of peripheral populations, as these play a significant role in the development of conservation strategies for kiangs. Based on field transect data collected from July to August 2023, this study predicted the suitable habitat distribution of kiangs in Shiqu County using a MaxEnt model and estimated the population size and density using the ‘Distance’ package. Additionally, it analyzed the responses of the group size of kiangs to environmental factors in Shiqu County. The results showed that the area of suitable habitat for kiangs is 3402.45 km2, accounting for 13.51% of the total area of Shiqu County. The estimated population was 1395.00 ± 272.20, with a population density of 0.41 ± 0.08/km2. Additionally, the group size of kiangs was significantly positively correlated with the distance from the road and grazing site. The distance from the grazing site, elevation, and temperature annual range are the main environmental factors affecting the distribution of kiangs. This study initially revealed the peripheral population Status and suitable habitat of the kiang on the eastern Tibetan Plateau, and the potential threat of grazing interference and road obstruction. The research results can provide a scientific reference for the population and habitat protection of kiangs in this area.

Factors Affecting Financial Losses Caused by Wild Boars in Ningxia, China

There is a need to reduce human–wildlife conflicts in the area around Liupanshan Nature Reserve in Ningxia Hui Autonomous Region, China. This study investigated the financial losses caused by wild boar and their causes. A questionnaire investigation (n = 135) and a field test were conducted, which included 108 sample lines and 97 infrared cameras. A principal component analysis and generalised linear model was used to analyse the importance of the effect of the factors on wild boar damage. Based on an estimate of 17,049 wild boars in the study area, we found that in the agricultural land owned by the residents, the boar density of each county and distance from the village to the nature reserve were the most significant factors that affected crop damage. Then, financial losses in spring, summer, and autumn had a moderate effect on financial loss, and the crop type had the lowest effect. We recommend reducing the wild boar population by increasing leisure hunting and the number of leopards. Additionally, a focus on farmland protection is a practical way to prevent wild boar invasions. Meanwhile, it is also necessary to conduct long-term monitoring of wild boar population status and manage the relationship between the government, research teams, and local people to more efficiently and comprehensively reduce conflicts between humans and wild boars.

Length in assessing status of freshwater fish populations: A review

AbstractObjectiveEffective policy formulation regarding the conservation of freshwater fish necessitates an understanding of water‐specific prevailing conditions and trends. Assessing fish populations in inland waters is difficult and expensive because there are many independent systems that need to be evaluated. Therefore, numerous freshwater systems are beset by insufficient data and the lack of systematic assessments of their status. To alleviate this deficiency, the objective of this study was to review length‐based metrics that may have utility in evaluating the well‐being of freshwater fish populations.MethodsLength measurements can serve as proxies for a range of ecological and population dynamics attributes that are essential for the effective management of fish and associated fisheries. A review of the historical development of length measurements in fish conservation is provided, along with an examination of the potential biases that may arise from the use of lengths in practical contexts. In addition, we examine techniques that enable the spatial and temporal visualization of length data sets, as well as a range of indices and metrics that can be computed using length measurements.ResultBuilding populations assessments around length may be a cost‐effective strategy that allows a first cut at managing a large number of waters. Length‐based assessments can signal if management intervention is necessary, if management policies are yielding the intended outcome, or if surveys beyond mere length are necessary.ConclusionOur review indicates that length offers a straightforward and efficient approach to evaluate the status of fish populations in inland systems. We encourage pursuing additional study and to this end propose specific areas for investigation.

Wolbachia infection in natural mosquito populations from Argentina.

The increasing spread of mosquito vectors has made mosquito-borne arboviral diseases a global threat to public health, leading to the urgent need for effective population control methods. Strategies based in the intracellular bacterium Wolbachia Hertig, 1936 are considered environmentally friendly, safe for humans, and potentially cost-effective for controlling arboviral diseases. To minimize undesirable side effects, it is relevant to assess whether Wolbachia is present in the area and understand the diversity associated with native infections before implementing these strategies. With this purpose, we investigated Wolbachia infection status, diversity, and prevalence in populations of Aedes albifasciatus (Macquart, 1838), Aedes fluviatilis (Lutz, 1904), and hybrids of the Culex pipiens (Linnaeus, 1758) complex from Argentina. Aedes albifasciatus and C. pipiens complex samples were collected in the province of Buenos Aires, and A. fluviatilis in the province of Misiones. Aedes albifasciatus was found to be uninfected, while infections with strains wFlu and wPip were detected in A. fluviatilis and hybrids of the C. pipiens complex, respectively. All strains were fixed or close to fixation and clustered within supergroup B. These finding provides valuable information on Wolbachia strains found in natural mosquito populations in Argentina that might be used in heterologous infections in the future or be considered when designing control strategies based on Wolbachia infection.

Development and sensory evaluation of Moringa oleifera–enriched snack bar for micronutrients deficiency in adolescents

Background: This study aimed to assess the sensitivity, texture, and nutritional quality of Moringa-enriched wheat bars to improve the nutritional status of the adolescent population. Moringa, which has gained a nutraceutical perspective from various pieces of research, is being incorporated into food product development. Moringa leaves from the “miracle tree,” a habitat of origin from South Asia, are enriched with macro- and micronutrients that combat both malnutrition and hidden hunger (micronutrient deficiency). The phytochemical properties of Moringa leaves, such as their antidiabetic, anticancer, antiulcer, antimicrobial, and antioxidant properties, make them a superfood. Purpose: This study aimed to develop a snack bar incorporating Moringa flour that aligns with adolescents’ snacking preferences while also providing an adequate supply of iron. Materials and Methods: Moringa leaves were processed using the conventional method to obtain sieved, coarse Moringa leaf flour. Moringa oleifera snack bars were prepared at concentrations of 15% (M1), 10% (M2), and 5% (M3). The snack bar that shows high acceptability was subjected to nutritional evaluation to determine the specific iron content. Results: Organoleptic evaluation of M3, M2, and M1 by 24 semitrained personnel identified M3, at a 5% concentration, as the most acceptable. The nutritionally analyzed M3 M. oleifera snack bar contained protein (12.1 g), fiber (9.18 g), iron (10.1 g), magnesium (112 mg), calcium (345 mg), zinc (19.4 mg), and a good source of energy (447 kcal). Discussion: Moringa, as a nutraceutical agent, in addition to its average dietary value utilized in nutritional snack bar development, aids in health promotion and disease prevention among adolescents with unhealthy food habits, such as consuming junk food. Conclusion: The M. oleifera snack bar supplies the recommended dietary allowance of micronutrients and macronutrients and contains antioxidants, such as flavonoids.

Lessons for Strengthening a Resilient Health System from the View of Health Facilities During the COVID-19 Pandemic: A Qualitative Study.

Coronavirus disease 2019 caused significant negative damage to the health status of populations and health systems globally. In Uganda, our previous study revealed that the strict Public Health and Social measures (PHSMs) and the closure of Entebbe Regional Referral Hospital (ERRH) led to missed healthcare access among the citizens in the Entebbe municipality. Limited studies, however, exist on the impact of the response measures on the local health systems. This study aims to explore the impacts of these measures on the local health service provision from the views of health facilities in Entebbe. We used a thematic framework method, grounded by the health systems resilience framework consisting of five components: (1) health service delivery; (2) medical products and technology; (3) health workforce; (4) public health functions; and (5) governance and financing. Key informant interviews with eight representatives from four private hospitals and four health centers were conducted from September to October 2022. Fifteen themes and 25 subthemes were identified. With the closure of the ERRH and the strict PHSMs, the citizens faced various difficulties in accessing the needed health services. The facilities received an overwhelming number of patients and faced various challenges, such as a lack of medicine, healthcare workers, facility capacity, and no means to transfer patients. Nevertheless, the facilities made efforts to maintain the required services. Moreover, mobilizing vertical and horizontal actors through a flexible network, from the district health office to community health volunteers, helped to coordinate the medicines, transportation for both patients and healthcare workers, conduct patient tracking, etc. Our study suggested the importance of an integrated system of public health and health service delivery systems, the formalization of a vertical cooperative mechanism, and the introduction of public health insurance for strengthening resilient health systems. These insights may benefit other sub-Saharan cities.