The population-wide properties and demographics of extragalactic X-ray binaries (XRBs) correlate with the star formation rates (SFRs), stellar masses (M ⋆), and environmental factors (such as metallicity, Z) of their host galaxy. Although there is evidence that XRB scaling relations (L X/SFR for high-mass XRBs (HMXBs) and L X/M ⋆ for low-mass XRBs) may depend on metallicity and stellar age across large samples of XRB-hosting galaxies, disentangling the effects of metallicity and stellar age from stochastic effects, particularly on subgalactic scales, remains a challenge. We use archival X-ray through IR observations of the nearby galaxy NGC 300 to self-consistently model the broadband spectral energy distribution and examine radial trends in its XRB population. We measure a current (<100 Myr) SFR of 0.18 ± 0.08 M⊙ yr−1 and stellar mass M ⋆ = (2.15−0.14+0.26)×109 M⊙. Although we measure a metallicity gradient and radially resolved star formation histories that are consistent with the literature, there is a clear excess in the number of X-ray sources below ∼1037 erg s−1 that are likely a mix of variable XRBs and additional background active galactic nuclei. When we compare the subgalactic L X/SFR ratios as a function of Z to the galaxy-integrated L X-SFR-Z relationships from the literature, we find that only the regions hosting the youngest (≲30 Myr) HMXBs agree with predictions, hinting at time evolution of the L X–SFR–Z relationship.