In the pursuit of sustainable turfgrass management for golf courses, a series of experiments was conducted to assess the potential of Trichoderma TrB (CNCM strain I-5327) as a natural solution. The research encompassed greenhouse and field trials across two golf courses. The comprehensive pot experiment investigated Trichoderma effectiveness, including native (TrB) and commercial strains, with and without organic amino acids, for turfgrass and soil health. The study followed a two-stage process, stimulating beneficial microorganisms with TrB and introducing Fusarium for biocontrol. Preliminary field trial on one golf course utilized a randomized block design to examine the effects of TrB and Trianum with amino acids, and fungicide on soil microbial community. A second field trial analyzed soil metabolic profiles after applying TrB, Trianum, and fungicide on another golf course. The greenhouse experiments demonstrated promising outcomes from the application of TrB, especially when combined with organic amino acids. This combination not only promoted plant growth and improved soil health but also effectively prevented the activation of Fusarium. In both field trials, it was observed that the introduction of TrB into the soil led to an increase in the population of soil fungi and bacteria and stimulated their activity. Our field data revealed that enriching the soil with TrB had a positive effect on soil microbial communities, while the application of fungicide resulted in a decrease in microbial activities. In summary, our research underscores Trichoderma's potential (TrB) in sustainable golf course management. These findings highlight TrB as a promising natural solution for improving turfgrass health and soil quality in sustainable management of golf course.