Assess the prevalence of temporomandibular dysfunction (TMD) in adults in Jeddah, Kingdom of Saudi Arabia. This was a cross-sectional study of adults aged 18 years and above during their routine visit to primary care dental centers in the city in 2018. Five-hundred participants enrolled in this study. Primary healthcare centers are clustered geographically in five areas of the city (north, south, west, east, and center) by the ministry of health. One-hundred participants were recruited from each area. The TMD/pain screener questionnaire was completed by participants. The first item used a scoring mechanism of 0-2 (a = 0, b = 1, c = 2), and the second and third items were scored 0-1 (a = 0, b = 1). A total score of 3 or more indicates the presence of TMD. t test was used to evaluate gender differences in TMD prevalence with a significance level of p < 0.05. There were 250 females and 250 males participants. The mean age was 40.6 years (range: 18-89 years; standard deviation: 13.7 years). Answers to the first question showed that 52% of participants had no pain in the temple area, or jaw in the last 30 days, 46% of participants had intermittent pain, and 2% had persistent pain. Twenty-seven percent of participants had pain or stiffness in the jaw upon waking up, whereas 39% of participants had pain while chewing food, 29% experienced pain from opening the mouth or moving the jaw, 34% during jaw habits such as holding the teeth together, clenching, or chewing gum, and 18% during other jaw activities such as talking, kissing, or yawning. A total score of 3 or more was found in 175 participants, indicating a prevalence of 35%. TMD was significantly more prevalent in females (105 participants, 42%) than in males (70 participants, 28%) with p = 0.0008. The prevalence of TMD in adult population in Jeddah is considered high. It is significantly more prevalent in females than in males. TMD prevalence is relatively high in adults of Jeddah, Kingdom of Saudi Arabia. Public health efforts should be directed at increasing TMD awareness in patients and healthcare professionals.