ObjectivesMentha piperita, generally known as peppermint is a popular herb cultivated in the temperate region around the world belongs to the family Lamiaceae. M. piperita is widely used as traditional food flavoring, in addition they are as well used to treat cold, fever and throat inflammation, and they have anti-viral and anti-fungal properties. The characteristics of MicroRNAs (miRNAs) includes, small size with single stranded non-coding regions having 22 nucleotides. They are evolutionary conserved and actively involved in post-transcriptional regulation of RNAs, which plays various important roles in metabolic and cellular processes of animals and plants. MethodsThe present study reports the potent computational method based on homology search used to find potential miRNA of peppermint. EST sequences were retrieved from NCBI GenBank and BLAST against with plant miRNAs. By various filtration criteria we identified potential miRNAs from miRNA family with suitable properties. Results and ConclusionA total of 1216 ESTs were and 1188 unigenes were identified in this study. A set of prospective target genes were identified in peppermint based on sequence homologies mainly encodes for transcription factor, transport inhibitor like protein, GRR1-like protein and DNA-binding protein. The present study endows valuable information on miRNA in peppermint which will accelerate further research in peppermint.