Pond Level Research Articles

Climatic controls on groundwater–surface water interactions within the Boreal Plains of Alberta: Field observations and numerical simulations

Hydrologic data were collected over eleven years at a catchment situated in low permeability glacial terrain within the Boreal Plains (Alberta, Canada) to evaluate the hydrologic interactions occurring between landscape units (i.e., shallow pond, extensive peatlands, aspen forested hillslopes). Two-dimensional numerical models were developed using a fully-integrated groundwater–surface water model to evaluate key landscape features that allow these ecosystems to persist within the sub-humid climate. Study results show that the dynamic interactions between the pond and peatlands are driven by precipitation and evapotranspiration. As a result, pond and peatland water levels reflect recent climatic trends. Limited hillslope contributions to the peatlands occur, indicating they are not required within this climatic setting for long-term maintenance. Instead, the peatlands conserve water within the landscape and supply it to adjacent landscape units. By contrast, the pond and the aspen forested hillslopes are dominated by high rates of evapotranspiration, and represent net water sinks within the landscape. Further simulations indicate these hydrologic systems are sensitive to pond and peatland evapotranspiration rates, and the hydraulic conductivity of the underlying glacial till substrate.

Infiltration into Two-Layered Soil: The Green–Ampt and Averyanov Models Revisited

Infiltration into a two-layered soil (continuum scale) is studied by the Green–Ampt (GA) model where the rate of infiltration (wetting front propagation) is a non-monotonic and discontinuous function of time. Both a constant ponding depth and a ponding level falling due to evaporation and infiltration are studied theoretically and in column experiments. The triads of saturated conductivity, porosity and front pressure in the two zones, as well as gravity, control vertical infiltration. Another model on the scale of a straight cylindrical capillary uses the Averyanov regime of a film flow, modified to include a front which is a quarter of a torus, rather than a spherical cap in a standard Washburn–Lukas model. The rate of front propagation is controlled by the Newtonian viscosity, tube-film sizes, surface tension, contact angle and gravity. For both scales, Cauchy problems for ODEs with respect to the front position are solved by computer algebra integration. In the field, pedon description and textural analysis of sedimentation-controlled layering are done. Of particular note is the thick cake deposited from a flash flood water pulse, which has very low permeability and can greatly inhibit infiltration. The infiltration rates measured by tension infiltrometers fluctuate, which is attributed to the mosaic and layered special distribution of hydraulic and capillary properties of the accrued sediments. Falling ponding depth laboratory experiments were conducted in four columns with a coarse substratum (sand) subjacent to a layer of thoroughly homogenized repacked dam silt. Imbibition front stopped at the interface between two texturally contrasting strata and the “waiting time” of the capillary barrier breakthrough was measured, as well as further oscillations of the infiltrations rate.

Yearly, pond, lineage and family variation of hepatopancreatic parvo-like virus (HPV) copy number in banana shrimp Fenneropenaeus merguiensis

Hepatopancreatic parvo-like virus (HPV) has been reported from a variety of shrimp species around the world, including Australia, and thought to impact negatively on production, but until now there was scant information available on variation of HPV over time, ponds and shrimp lineages or families, information that could be used to manage or reduce virus levels.Here we report HPV copy number estimated using qPCR from 1500 individual shrimp sampled over three years and encompassing 91 ponds, 21 breeding groups or lineages and 40 families.HPV copy number variation between ponds was used by farm management as a criterion to choose prospective broodstock (candidates were taken from low HPV ponds). Despite such choice, HPV levels in farmed animals were not reduced from 2011 to 2013. Accordingly, the hypothesis that HPV levels can be reduced over time simply by considering average HPV levels in ponds alone is rejected.Different lines of shrimp within the same farm had different HPV levels, but as lines were raised separately, the line differences could be due to either genetic or environmental differences, the latter including possible different rearing effects and differences in vertical transmission.There were large (up to 2–3 LOG fold) differences of HPV levels between families bred and grown together contemporaneously, and the heritability for HPV copy number was estimated to be moderate to large (0.40±0.13). Apart from genetic differences, differences of vertical transmission from dams may contribute to the between family differences, in any case we postulate that selection between families could be an effective method to reduce HPV levels.HPV levels were not genetically correlated with performance traits such as body weight or length, so selection for HPV level should not adversely affect production characteristics.This is the first evidence for an aquacultured species that viral levels, as opposed to survival/resistance to viruses, may have a substantial host genetic component. The heritability reported here for virus copy number was higher that most heritabilities reported for survival to specific pathogens such as white spot, raising the general postulate that selection for virus copy number may be more effective and repeatable than selection for survival to pathogen challenge.

Landscape context influences chytrid fungus distribution in an endangered European amphibian

AbstractWildlife disease is an emerging threat to biodiversity. The amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd), which causes the disease chytridiomycosis, has been documented in over 500 amphibian species globally. Understanding conditions under which amphibians are vulnerable to Bd is important for evaluating species risk and developing surveillance strategies. Here, we investigate the spatial distribution of Bd infection in the ephemeral pond‐breeding yellow‐bellied toad Bombina variegata, a species of high conservation concern in the European Union. We sampled 550 toads from 60 ponds in a traditional agricultural landscape in Southern Transylvania, Romania. Overall, Bd prevalence was low in B. variegata, but infected toads were widely dispersed through the landscape and were found in a quarter of all sampled ephemeral ponds. At the pond level, increased Bd occurrence was associated with short distances to perennial water sources and high forest cover. These findings suggest that perennial water sources may act as source habitat for Bd, with amphibian movements resulting in Bd spillover into ephemeral ponds. Increased Bd occurrence in ponds surrounded by high levels of forest cover is likely related to cooler and wetter conditions that are more favourable for Bd. Throughout the study landscape, patchy environmental suitability for Bd appears to restrict the pathogen to a subset of B. variegata habitat. Ephemeral ponds in open landscapes, without nearby perennial habitat, likely provide an environmental refuge from Bd, where the risk of infection is low. From a conservation perspective, these findings highlight the importance of maintaining ephemeral ponds in open landscapes, but these are currently threatened by land‐use change.

Tailings Subaerial and Subaqueous Deposition and Beach Slope Modeling

The tailings beach slope is a fundamental parameter for the design and operation of tailings storage facilities because it affects the tailings storage capacity, pond levels, and phreatic surfaces within the containment structures. Understanding tailings beach development is particularly important for thickened and paste tailings facilities, where the tailings can form a self-stacking structure. This paper presents a theoretical tailings deposition model that can describe the formation of tailings beach slopes in both subaerial and subaqueous deposition environments. The depositional behavior and deposition mechanisms are investigated from both geotechnical and hydraulic engineering perspectives. The model is developed to capture deposition mechanisms based on the principles of soil mechanics and fluid mechanics. The governing differential equations can be solved using a numerical technique, and the solution scheme can readily be implemented in computer languages. Case studies for subaerial deposition demonstrate that the modeling results are in agreement with laboratory flume testing and field measurements. The proposed model considers geotechnical and rheological properties of tailings, water pressures, operation parameters, and site topographic conditions. A sensitivity study was carried out to provide insight into the effects of influencing factors on the beach slope.

Groundwater fluxes in a shallow seasonal wetland pond: The effect of bathymetric uncertainty on predicted water and solute balances

The successful management of groundwater dependent shallow seasonal wetlands requires a sound understanding of groundwater fluxes. However, such fluxes are hard to quantify. Water volume and solute mass balance models can be used in order to derive an estimate of groundwater fluxes within such systems. This approach is particularly attractive, as it can be undertaken using measurable environmental variables, such as; rainfall, evaporation, pond level and salinity. Groundwater fluxes estimated from such an approach are subject to uncertainty in the measured variables as well as in the process representation and in parameters within the model. However, the shallow nature of seasonal wetland ponds means water volume and surface area can change rapidly and non-linearly with depth, requiring an accurate representation of the wetland pond bathymetry. Unfortunately, detailed bathymetry is rarely available and simplifying assumptions regarding the bathymetry have to be made. However, the implications of these assumptions are typically not quantified. We systematically quantify the uncertainty implications for eight different representations of wetland bathymetry for a shallow seasonal wetland pond in South Australia. The predictive uncertainty estimation methods provided in the Model-Independent Parameter Estimation and Uncertainty Analysis software (PEST) are used to quantify the effect of bathymetric uncertainty on the modelled fluxes. We demonstrate that bathymetry can be successfully represented within the model in a simple parametric form using a cubic Bezier curve, allowing an assessment of bathymetric uncertainty due to measurement error and survey detail on the derived groundwater fluxes compared with the fixed bathymetry models. Findings show that different bathymetry conceptualisations can result in very different mass balance components and hence process conceptualisations, despite equally good fits to observed data, potentially leading to poor management decisions for the wetlands. Model predictive uncertainty increases with the crudity of the bathymetry representation, however, approximations that capture the general shape of the wetland pond such as a power law or Bezier curve show only a small increase in prediction uncertainty compared to the full dGPS surveyed bathymetry, implying these may be sufficient for most modelling purposes.

Backwater profile during barrage gate operation for variable pond levels

The backwater profile plays an important role in the design and performance of any hydraulic structures. Various methods are already in practice for the estimation of backwater profile. Present study attempts to estimate the backwater profiles due to barrage gate operations considering variable pond levels for alluvial channels using both theoretical analysis and laboratory model tests. The physical model tests were conducted for different pond levels and discharges. Backwater profiles obtained from theoretical and physical model tests data were compared with those obtained from available methods. Finally, a non-dimensional relationship has been established between the pond level and the backwater length.

Modeling the Hydrological Dynamic of the Breeding Water Bodies in Barkedji’s Zone

Temporary water bodies’ dynamics play an important role in the epidemiological chain-borne diseases such as Rift Valley fever as they are the main breeding habitats for mosquitoes. During the rainy season, hundreds of these temporary water bodies appear and grow in the Ferlo region (Senegal). The purpose of this research is to generate historical and future time series water levels and areas at three temporary ponds located in the environment and health observatory of Barkedji. A simple lumped hydrological model was developed for that purpose. It describes each pond watershed as three interconnected reservoirs: canopy, surface storage and soil storage and uses a linear relation to describe infiltration, percolation and baseflow (out of the soil reservoir). Given the depth of the water table in the region, percolation out of the soil surface is considered lost. Evapotraspiration was calculated using the Penman equation and withdraws water from the canopy and surface water reservoirs. Excess runoff from the soil storage is turned into runoff using a triangular unit hydrograph. The calibration was done using two years of hydrological and climatic data collected during the 2011 and 2012 rainy seasons. The calibration was successful and water level in the two ponds was simulated with a Root Mean Square Error (RMSE) of 11.2 to 15 cm. Because of the short duration of the observation, no validation could be done. Given the excellent agreement of the simulated and observed water levels during the calibration phase, the modeling exercise was considered to be successful. The developed models were used to generate historical time series of pond areas and correlate these to mosquitoes’ infestation in the region. Future time series of pond areas were also generated using downscaled outputs of three regional climate models from the AMMA ENSEMBLES experiment. The generated pond levels and areas are being used to assess the evolution of the disease in the next 40 years.

Time-Activity Budgets of Waterfowl Wintering on Livestock Ponds in Northeast Texas

We developed individual time-activity budgets for Anas platyrhynchos (Mallard; n = 281), A. strepera (Gadwall; n = 251), and Aythya collaris (Ring-necked Duck; n = 144) wintering on livestock ponds in the Blackland Prairies Ecological Region of Texas in January and February, 2000 and 2001. Feeding (32–38%), locomoting (24–49%), and resting (10–36%) dominated the activity budgets for each species. Behaviors varied between years, probably due to the 3-fold increase in precipitation that raised water levels in livestock ponds. In 2000 and 2001, Mallards fed nearly 50% and 20% of their time, respectively, with comfort and resting occupying 60% in 2001. Gadwalls locomoted nearly 50% of their time each year, but increased surface feeding 2-fold in 2001. Finally, Ring-necked Ducks spent about a third of their time locomoting, another third resting, and the remainder subsurface feeding in 2001. Focal species activity budgets were generally similar to those developed throughout their ranges. Livestock ponds in northeast Texas provide small but regionally widespread habitats for wintering waterfowl. Future work should focus upon diet and landscape occupancy rates of waterfowl using these habitats during winter.

The episodic and abrupt geochemical changes at La Fossa fumaroles (Vulcano Island, Italy) and related constraints on the dynamics, structure, and compositions of the magmatic system

Herein we report on the chemical and isotopic (C, H, O, and He) compositions of the fluids from La Fossa crater fumaroles of Vulcano from 1999 to 2010. Consistent with records obtained since the end of the 1980s, our data show that the geochemical features of the fumarole system have experienced several episodes of remarkable change, each lasting no more than a few months. Typical signatures of these short-term anomalies are large increments in CO2, N2, and He concentrations, coupled to increased 13C/12C isotopic ratios, but their meaning remains widely debated. Within a model of fumarolic fluids based on mixing between hydrothermal and magmatic endmembers, we have developed a novel approach to constrain chemical (He/CO2 and N2/He) and isotopic (13C/12C, D/H, and 3He/4He) ratios of the magmatic endmember during the short-term anomalies. Although much of the geochemical variability in fumaroles results from changes in mixing proportions, the magmatic fluid unquestionably shows significant variations in time. The magmatic He/CO2, N2/He, 13C/12C, and 3He/4He values throughout 1988–1996 differed from those feeding the anomaly at the end of 2004. Early clues of the new magmatic fluid appeared in 1998–1999, far from any short-term anomaly, whereas new and old magmatic fluids coexisted after 2004. We quantitatively prove that the detected geochemical changes are consistent with the degassing path of a magma having a latitic composition, and suggest the presence of two magma ponding levels at slightly different pressures, where bubble–melt decoupling can occur. The different He-isotope compositions at these levels suggest low hydraulic connectivity typical of a complex reservoir with dike and sill structures. In this framework, the short-term geochemical anomalies are probably due to gas accumulation at the top of magma bodies followed by massive escape, or activation of new degassing levels in the reservoir, for which the stress field almost certainly plays a key role. Such a scenario explains the observed increases in both fumarole output and shallow high-frequency seismicity (due to increased pore pressure) during the anomalies, while being consistent with the concomitant absence of any deep seismicity or ground deformation, eventually related to magma movement.

An expert-based landscape permeability model for assessing the impact of agricultural management on amphibian migration

The persistence of pond-breeding amphibian populations is influenced on different spatial scales ranging from the individual breeding pond to surrounding habitat patches to landscape clusters of breeding pond populations. The connectivity among breeding ponds as well as the availability of suitable terrestrial habitats surrounding the ponds plays a major role in long-term viability of amphibian species. Besides road traffic and urban structures agricultural land activity can disrupt landscape connectivity through the use of pesticides, fertilizers and physical activity such as tillage. We developed an expert-based model to assess the impact of agricultural management measures on the migration area and terrestrial habitat availability for seven amphibian species. The model is based on a Habitat Suitability Index to identify suitable terrestrial habitats and includes a landscape permeability approach. Size of migration areas, connectivity of breeding ponds and number of reachable terrestrial habitats were modeled considering species-specific migration ranges and habitat preferences. We consider how pesticides application might lead to fragmentation and isolation of amphibian breeding pond populations. Therefore the potential disrupting impact of pesticides was simulated by inflating landscape resistance to medium and high migration cost. One amphibian species showed a decrease of migration area by 48.3% and a decrease of reachable terrestrial habitats by 41.5% at high migration costs. Three additional species showed a decrease of their migration areas between 31.5 and 35.7%. At increased migration cost, some of the investigated populations were isolated at breeding pond level or restricted to pond clusters. Our model could be used to prioritize conservation efforts for pond-breeding amphibians with adequate consideration of agricultural land use and its impact on amphibian migration.

Abnormalities in amphibian populations inhabiting agroecosystems in northeastern Buenos Aires Province, Argentina

The occurrence of abnormalities in amphibians has been reported in many populations, and its increase could be related to environmental pollution and habitat degradation. We evaluated the type and prevalence of abnormalities in 5 amphibian populations from agroecosystems with different degrees of agricultural disturbance (cultivated and reference areas). We detected 9 types of abnormalities, of which the most frequent were those occurring in limbs. The observed prevalence of abnormality in assessed populations from cultivated and reference areas was as follows: Rhinella fernandezae (37.1 and 10.2%, respectively), Leptodactylus latrans adults (28.1 and 9.2%) and juveniles (32.9 and 15.3%), and Hypsiboas pulchellus (11.6 and 2.8%). Scinax granulatus populations did not show abnormalities. Pseudis minuta, which was only detected in the reference area, exhibited a prevalence of 13.3%. For R. fernandezae, L. latrans, and H. pulchellus, generalized linear mixed models showed that prevalence of abnormalities was significantly higher (p < 0.05) in cultivated than in reference areas. L. latrans juveniles were more vulnerable to abnormalities than adults (p < 0.05). The presence of abnormalities in some species inhabiting different agroecosystems suggests that environmental stress factors might be responsible for their occurrence. While we detected pesticides (endosulfan, cypermethrin, and chlorpyrifos) and lower dissolved oxygen levels in ponds of the cultivated area, no data are currently available on how other factors, such as injuries from predators and parasite infections, vary by land use. Further research will be necessary to evaluate possible causes of abnormalities detected in the present study mainly in the context of factor interactions.

Level-dependence of the relationships between amphibian biodiversity and environment in pond systems within an intensive agricultural landscape

Wetlands, especially ponds, and their associated amphibian biodiversity are threatened by agricultural intensification. To improve conservation planning of these ecosystems, we need to understand at which scales biodiversity responds to human-induced disturbances. This study aims to assess the level-dependence of environment-amphibian biodiversity relationships in 150 ponds in an intensive agricultural landscape in Seine-et-Marne (France). Amphibian diversity surveys, site characteristic measurements and landscape descriptions are analysed. The hierarchy of the effects of local and regional variables on species richness, regional heterogeneity of species composition and species occurrences is investigated at three spatial levels: pond level, 1-, and 4-km(2) level. Species richness is negatively influenced at all levels by the fish presence. Water quality and pond density, which emphasize level-dependent effects, significantly increase species richness at the local and regional levels, respectively. With few exceptions, species occurrence analysis shows similar patterns, confirming, locally, the importance of fish avoidance, and, regionally, the need for increasing pond density. Environmental variables have no effect on the regional heterogeneity of species composition, questioning the potential existence of dispersal processes at scales above 1 km(2). This study highlights the relevance of a pond-group-centred approach compared to a pond-centred approach with regard to pond conservation in agricultural landscapes.

Effects of salinity and clipping on biomass and competition between a halophyte and a glycophyte

Global climate change will likely result in the reduction of water levels in intermountain wetlands and ponds, and the vegetation communities associated with these wetlands are an important forage source for livestock. Lowered water levels will not only constrict wetland plant communities, it will potentially change aquatic and soil salt concentrations. Such an increase in salinity can reduce plant growth and potentially affect competitive interactions between plants. A greenhouse experiment examined the effects of salinity and competition on the growth of two wet meadow grass species, Poa pratensis (a glycophyte) and Puccinellia nuttalliana (a halophyte). The following hypotheses based on published data were tested: (1) Biomass of both species will decrease with increasing concentration of salt; (2) root:shoot (R:S) ratio of P. pratensis will decrease with increasing salt concentration while R:S ratio of P. pratensis and P. nuttalliana will increase with clipping; (3) competitive importance will decrease for P. pratensis and P. nuttalliana with increasing salt concentration because salt induces a stress response and competitive importance is reduced in stressed environments. A factorial design included 3 plant treatments (P. nuttalliana alone, P. pratentsis alone, P. nuttalliana + P. pratensis) × 4 salinity rates (control; 5, 10, 15 g/L NaCl) × 2 clipping intensities (plants clipped or not clipped) for a total of 24 combinations replicated 6 times over a period of 90 days. We found a reduction in dry biomass as salinity increased, and this effect was greatest for P. pratensis. (1.94 g (SE 0.13) at 0 g/L NaCl to 0.22 g (SE 0.11) at 15 g/L NaCl). The R:S ratio of P. pratensis was reduced by salinity, but not for P. nuttalliana. Competitive importance of both species was reduced by clipping and by salinity, but the effect was greater and more consistent for P. pratensis. We conclude that salt concentration reduces plant growth and the effect of competition.

Taming the Beast: Managing Hydrology to Control Carolina Willow (Salix caroliniana) Seedlings and Cuttings

AbstractHistorically, wetlands along the St. Johns River, Florida, were dominated by herbaceous marshes. However, in the last 50 years many areas transformed to shrub‐dominated wetlands, at the same time a system of levees and canals was constructed to control flooding. We tested the role of water management in controlling Carolina willow (Salix caroliniana), a native shrub that accounts for most of this shift. We assessed survival and growth of seedlings and cuttings on four artificial islands. We planted willow seedlings and cuttings at the spring waterline and at three higher levels (+17.5, +35, and +50 cm) and evaluated their responses to natural hydrologic fluctuations. Overall, seedlings had lower survival than cuttings. Highest mortality occurred during summer floods and willows greater than 50 cm above marsh surface had the highest survivorship. Surviving seedlings attained similar height and biomass among elevations, but the cuttings had greater stem diameter, stem height, and biomass at higher elevations. In the second experiment, we planted seedlings and short (25 cm) and tall (50 cm) cuttings at the waterline and at three higher levels (+25, +35, and +50 cm) in artificial ponds with controlled water levels. Before flooding, seedlings at the highest elevation suffered some mortality due to desiccation, but after flooding, they had the highest survival. Elevation did not affect cutting survival, but those at the lowest elevation had the greatest height and biomass. Hydrologic manipulation can be a powerful tool to control willow establishment. However, its success depends on timely and prolonged inundation or water drawdown.

Identifying landscape features associated with Rift Valley fever virus transmission, Ferlo region, Senegal, using very high spatial resolution satellite imagery.

IntroductionDynamics of most of vector-borne diseases are strongly linked to global and local environmental changes. Landscape changes are indicators of human activities or natural processes that are likely to modify the ecology of the diseases. Here, a landscape approach developed at a local scale is proposed for extracting mosquito favourable biotopes, and for testing ecological parameters when identifying risk areas of Rift Valley fever (RVF) transmission. The study was carried out around Barkedji village, Ferlo region, Senegal.MethodsIn order to test whether pond characteristics may influence the density and the dispersal behaviour of RVF vectors, and thus the spatial variation in RVFV transmission, we used a very high spatial resolution remote sensing image (2.4 m resolution) provided by the Quickbird sensor to produce a detailed land-cover map of the study area. Based on knowledge of vector and disease ecology, seven landscape attributes were defined at the pond level and computed from the land-cover map. Then, the relationships between landscape attributes and RVF serologic incidence rates in small ruminants were analyzed through a beta-binomial regression. Finally, the best statistical model according to the Akaike Information Criterion corrected for small samples (AICC), was used to map areas at risk for RVF.ResultsAmong the derived landscape variables, the vegetation density index (VDI) computed within a 500 m buffer around ponds was positively correlated with serologic incidence (p<0.001), suggesting that the risk of RVF transmission was higher in the vicinity of ponds surrounded by a dense vegetation cover. The final risk map of RVF transmission displays a heterogeneous spatial distribution, corroborating previous findings from the same area.ConclusionsOur results highlight the potential of very high spatial resolution remote sensing data for identifying environmental risk factors and mapping RVF risk areas at a local scale.

Variation in Local Scour Profiles of an Embankment Pond

This paper intents to identify the variation in local scour profiles of an embankment pond under varying pond water depths. The embankment pond was constructed to regulate the water issuing from the tunnel of a hydropower station before releasing it to the river. To avoid the structural collapse of any hydraulic structure, the scour process is one of the primary factors that must be controlled. Sediments (soils and sands) are composed of aggregation of individual grains that have varying density, volume, shape and orientation. Therefore an investigation is quite important on interactions between the fluid and sediment particles in open and pipe channel flows in hydraulics and river engineering. The variation in pond water level has significant effect on the scour that may occur on the bed of the embankment pond. This paper highlights the variations and suggests for maintaining certain pond water level to lessen the scour hazards.

Prevalence and molecular characteristics of Vibrio spp. isolated from preharvest shrimp of the North Western Province of Sri Lanka.

This study investigated the prevalence and molecular characteristics of Vibrio spp. in farmed shrimp (Penaeus monodon) in Sri Lanka. A total of 170 shrimp samples (100 g of whole shrimp each) taken from individual ponds from 54 farms were collected 1 week prior to harvest from the North Western Province of Sri Lanka. Overall, 98.1% of the farms and 95.1% of the ponds were positive for Vibrio spp. in shrimp; at the pond level, V. parahaemolyticus (91.2%) was most common, followed by V. alginolyticus (18.8%), V. cholerae non-O1/non-O139 (4.1%), and V. vulnificus (2.4%). Multiple Vibrio spp. were detected in 20.6% of the ponds. None of the V. parahaemolyticus isolates (n = 419) were positive for the virulence-associated tdh (thermostable direct hemolysin) and trh (TDH-related hemolysin) genes. V. cholerae was confirmed by the presence of ompW, and all isolates (n = 8) were negative for the cholera toxin (ctxA) gene. V. cholerae isolates were serogrouped by PCR and identified as V. cholerae non-O1/non-O139. All four V. vulnificus strains, isolated from different ponds of two geographical regions, showed pathogenic potential; they belonged to vcgC sequence type, type B 16S rRNA genotype and contained a pilF polymorphism associated with human pathogenicity. The results of this study revealed the ubiquitous nature of vibrios in farmed shrimp. To minimize the potential risk of Vibrio infections due to handling or consumption of raw or undercooked seafood products, good manufacturing practices as well as proper handling and processing should be addressed.

High Degree of Mitochondrial Haplotype Diversity in the Japanease Common Toad Bufo japonicus in Urban Tokyo

The Japanese common toad Bufo japonicus is widely distributed across mainland Japan and is classified into two subspecies, B. japonicus japonicus and B. japonicus formosus, in the western and eastern regions, respectively. To investigate the genetic diversity of B. japonicus at the breeding pond (local population) level, we sequenced 831 base pairs of mitochondrial DNA (mtDNA) cytochrome b (Cyt b) from 75 individuals collected from nine ponds in urban Tokyo and the surrounding area. Phylogenetic and population genetic analyses revealed high mtDNA haplotype diversity (Hd, 0.716 (mean) ± 0.230 (SD)) within local populations (breeding ponds). Most local populations had multiple haplotypes of the mitochondrial Cyt b gene, and seven of the 18 haplotypes were identified in two or more local populations. These results indicate that mitochondrial gene flow had occurred across different breeding sites. We also identified five haplotypes that belonged to the western clade and correspond to B. japonicus japonicus. Our results provide genetic evidence that B. japonicus japonicus was introduced artificially from Western Japan to Tokyo, where it occupied the natural habitat of B. japonicus formosus. The urban Tokyo area was found to represent an admixed population consisting of both native and non-native B. japonicus subspecies.

Pond‐Level Risk Factors Associated with Columnaris Disease on Mississippi Commercial Catfish Farms

A large commercial catfish enterprise encompassing over 500 food fish ponds from five farms covering multiple counties in the Mississippi Delta was included in this analysis of columnaris risk factors. A gram-negative bacterium, Flavobacterium columnare, is the cause of columnaris disease and is considered the second-most prevalent bacterial disease in farm-raised catfish. The objective of this study was to determine if pond-level risk factors reported by farm personnel were associated with columnaris disease mortalities. To identify risk factors affecting susceptibility of farm-raised channel catfish Ictalurus punctatus to columnaris disease, a Catfish Management database was developed. Logistic regression was used to model the relationships between probability of columnaris in ponds and risk factors examined. Generalized linear mixed models incorporating hierarchically structured random effects of ponds and one or more fixed-effects risk factors were fitted. In the screening process, each risk factor was evaluated in the basic model as a single fixed-effects factor, and if associated with the outcome (P ≤ 0.20), was retained for development of multivariable models. Two multivariable logistic regression models were constructed from data collected at the pond level by producers. The first was constructed from data in which water quality was not considered. Pond depth and reduced feed consumption for a 14-d period prior to disease outbreaks measured on a per hectare basis were significantly (P ≤ 0.05) associated with columnaris disease. The second, in which water quality variables were also considered, pond depth, reduced feed consumption, shorter intervals from stocking to disease outbreaks, and total ammonia nitrogen were significantly (P ≤ 0.05) associated with columnaris occurrence. This study showed some commonly recorded production variables were associated with columnaris disease outbreaks and, if monitored, could help identify "at risk" ponds before disease outbreaks occur.