The energetic metabolism of perfused C6 glioma cells anchoted and cultured on polystyrene microcarrier beads has been studied by phosphorus-31 nuclear magnetic spectrocopy (NMR). The observation of in tracellular phosphorylated compounds demonstrates the metabolic long-lasting viability of the perfused cells. The effect of glucose deprivation on energetic metabolism and intracellular pH illustrates the existence of an active aerobic glycolysis. The non-invasive study of anchored C6 cells by NMR provides a direct means to investigate the metabolism of glioma cells.