Polysaccharide Moiety Research Articles

Deciphering the adsorption machinery of Deep-Blue and Vp4, two myophages targeting members of the Bacillus cereus group.

The Bacillus cereus group comprises closely related species, including some with pathogenic potential (e.g., Bacillus anthracis and Bacillus cytotoxicus). Their toxins represent the most frequently reported cause of food poisoning outbreaks at the European level. Bacteriophage research is undergoing a remarkable renaissance for its potential in the biocontrol and detection of such pathogens. As the primary site of phage-bacteria interactions and a prerequisite for successful phage infection, adsorption is a crucial process that needs further investigation. The current knowledge about B. cereus phage adsorption is currently limited to siphoviruses and tectiviruses. Here, we present the first insights into the adsorption process of Herelleviridae Vp4 and Deep-Blue myophages preying on B. cereus hosts, highlighting the importance of polysaccharide moieties in this process and confirming the binding to the host surface of Deep-Blue Gp185 and Vp4 Gp112 receptor-binding proteins and Gp119 baseplate wedge.

Stability and lipid oxidation of oil-in-water emulsion stabilized by Maillard conjugates of soybean phosphatidylethanolamine with different polysaccharides

Lipid oxidation and instability in oil-in-water (O/W) emulsion are the main challenges in emulsion-based foods. Polysaccharide-induced Maillard conjugation of soybean phosphatidylethanolamine (SP) holds great potential in improving physicochemical stability of emulsions. This work applied tamarind gum, pectin, inulin and maltodextrin to conjugate with SP to obtain the four Maillard conjugates (MCs), and investigated their difference in interface and anti-oxidant abilities in emulsions. Results showed that lower polymerization degree of polysaccharide moieties (tamarind gum＞pectin＞maltodextrin＞inulin) caused higher glycation degree in MC: inulin-SP-MC＞maltodextrin-SP-MC＞pectin-SP-MC ≈ tamarind gum-SP-MC, which corresponded their color changes. Compared with unconjugated mixtures, interface activity and anti-oxidant abilities of MCs were strengthened. Among the four MCs, tamarind gum-SP-MC (7.77 mN/m) and pectin-SP-MC (8.42 mN/m) could decrease interfacial tension more largely. It was due to the faster interface saturation benefited from broader steric network of tamarind gum and pectin moieties, and stable interfacial absorption facilitated by improved zeta potential and lipophilicity. Pectin-SP-MC also exhibited the highest anti-oxidant ability (DPPH scavenging ability of 97.81% and ferric-reducing antioxidant power of 2.742 A). It was attributed to the combined effects of grafting degree, interface activity and chain length of pectin moiety. As a result, emulsions stabilized by pectin-SP-MC had less water loss, smaller droplet sizes, and lower contents of oxidation products. Maillard reaction between SP and pectin was favorable to produce MCs with higher potential for the improvements of physical and oxidative stability of O/W emulsions.

Diverse sedimentary organic matter within the river-aquifer interface drives arsenic mobility along the Meghna River Corridor in Bangladesh

In alluvial aquifers with near-neutral pH and high dissolved arsenic (As) concentrations, the presence and character of sedimentary organic matter (SOM) regulates As mobility by serving as an energetically variable source of electrons for redox reactions or forming As–Fe-OM complexes. Near tidally and seasonally fluctuating rivers, the hyporheic zone (HZ), which embodies the mixing zone between oxic river water and anoxic shallow groundwater, may precipitate (or dissolve) iron (Fe)-oxides which sequester (or mobilize) As. To understand what is driving the mobilization of As within a shallow aquifer and riverbank sands adjacent to the tidally fluctuating Meghna River, we characterized the chemical reactivity of SOM from the sands, and a silt and clay layer, underlying the HZ and aquifer, respectively. Dissolved As (50–500 μg/L) and Fe (1–40 mg/L) concentrations increase with depth within the shallow aquifer. Similar vertical As and Fe concentration gradients were observed within the riverbank sands where concentrations of the products of reductive dissolution of Fe-oxides increase with proximity to the silt layer. Compared to all other sediments, the SOM in the clay aquitard contains older, more recalcitrant, terrestrially-derived material with high proportions of aromatic carboxylate functional groups. The shallow silt layer contains fresher SOM with higher proportions of amides and more labile polysaccharide moieties. The SOM in both the riverbank and aquifer is terrestrially-derived and humic-like. The labile SOM from the silt layer drives the microbially mediated reductive dissolution of As-bearing Fe-oxides in the HZ. In contrast, the carboxylate-rich SOM from the clay aquitard maintains dissolved As concentrations at the base of the aquifer by complexing with soluble As and Fe. This highlights that SOM-rich fine (silt or clay) layers in the Bengal basin drive As and Fe mobility, however, the specific processes mobilizing As and Fe depend on the lability of the SOM.

Effect of O antigen glycosyl isomerase gene mutation on biological property and pathogenicity of Burkholderia pseudomallei strain BPC006

Burkholderia pseudomallei, an intracellular pathogen, is responsible for melioidosis, a zoonotic disease. Its pathogenesis involves several virulence factors, among which lipopolysaccharide (LPS) plays a crucial role. Our research reveals that the O antigen present within the LPS significantly regulates the host immune response. In a previous study, we obtained a B. pseudomallei mutant strain ΔwbiI. Here, the purification of LPS from ΔwbiI and a gas chromatography–mass spectrometry (GC–MS) analysis were conducted. The results confirmed the absence of specific sugar 6-deoxy-Talp, which is a typical component of the O antigen in the wild type B. pseudomallei. Our findings underscore the potent impact the O antigen exerts on the virulence of B. pseudomallei. The ΔwbiI strain displayed significantly increased invasiveness and cytotoxicity in vitro. This enhanced cytotoxicity seems to be related to the exposure of lipid A and an increased cell membrane hydrophobicity resulting from the deletion of the O antigen. Additionally, in mouse models, the ΔwbiI strain resulted in a heightened host lethality and an excessive inflammatory response in mice. These findings indicate that the O-antigenic polysaccharide moiety of B. pseudomallei plays a role in its pathogenicity in vitro and in vivo.

Characterization of Mannoprotein Structural Diversity in Wine Yeast Species.

The structure of yeast cell wall (CW) mannoproteins (MPs) influences their impact on wine properties. Yeast species produce a diverse range of MPs, but the link between properties and specific structural features has been ill-characterized. This study compared the protein and polysaccharide moieties of MP-rich preparations from four strains of four different enologically relevant yeast species, named Saccharomyces boulardii (SB62), Saccharomyces cerevisiae (SC01), Metschnikowia fructicola (MF77), and Torulaspora delbrueckii (TD70), and a commercial MP preparation. Monosaccharide determination revealed that SB62 MPs contained the highest mannose/glucose ratio followed by SC01, while polysaccharide size distribution analyses showed maximum molecular weights ranging from 1349 kDa for MF77 to 483 kDa for TD70. Protein identification analysis led to the identification of unique CW proteins in SB62, SC01, and TD70, as well as some proteins shared between different strains. This study reveals MP composition diversity within wine yeasts and paves the way toward their industrial exploitation.

Exploring the influence of S. cerevisiae mannoproteins on wine astringency and color: Impact of their polysaccharide part.

The impact of the polysaccharide moiety of mannoproteins (MPs) on the color and astringency of red wines was studied respectively through spectrophotometry and their impact on tannin interactions with BSA. To this end, MPs with conserved native structures from four different Saccharomyces cerevisiae strains were used: a Wild-Type strain (BY4742, WT) taken as reference, mutants ΔMnn4 (with no mannosyl-phosphorylation) and ΔMnn2 (linear N-glycosylation backbone), and a commercial enological strain. MPs affected tannin-BSA interactions by delaying aggregation kinetics. To achieve it, a well-balanced density/compactness of the polysaccharide moiety of MPs was a key factor. MP-WT and MP-Mnn2 acted as weak copigments and induced a slight increase in the absorbance of Malvidin-3-O-Glucoside. The same MPs also promoted a synergistic effect during the copigmentation of Quercetin-3-O-Glucoside with Malvidin-3-O-Glucoside. The intensity of these hyperchromic effects was related to the accessibility of anthocyanins to negatively charged mannosyl-phosphate groups within the polysaccharide moiety.

Bacteriophages Concept and Applications: A Review on Phage Therapy.

The nature of phages was a matter of dispute, which was resolved in 1940, and it was continued to develop their activity and application in the Soviet Union and Eastern Europe. Bacteriophages were first employed in 1919 to treat bacterial illnesses caused by Citrobacter, Enterobacter, and Pseudomonas. Bacteriophages range in complexity from simple spherical viruses with genome sizes of less than 5 kbp to complicated viruses with genome sizes surpassing 280 kbp. They have two significant parts, head and tail, and are made up of numerous copies of more than 40 distinct proteins. Bacteriophages have been demonstrated to bind with receptors in the walls of both gram-positive and gram-negative bacteria, ranging from peptide sequences to polysaccharide moieties. Depending on the type of phage and the physiological state of the bacterium, the life cycle may diverge into the lytic cycle or lysogenic cycle. Lytic-lysogenic switch depends on a variety of inducing factors. Bacteriophage therapy can be administered via several routes, but parenteral routes are the most effective. Auto-dosing, single-dose potential, lack of cross-resistance with antibiotics, etc., are several advantages of phage therapy over antibiotic treatment. Bacteriophages are attracting much attention because of their potential advantages and wide applications as antibacterial agents, diagnostic technologies, phage-based products, and biocontrol agents. They also have several applications in the food industry, agriculture/crop, farm animal and bee protection, environmental, and biosensor development.

Augmented dye eradication from wastewater using alkali-aided, reinforced waste acacia (Acacia auriculiformis) leaves

The present investigation demonstrates the augmented dye scavenging from wastewater using alkali-mutated acacia (Acacia auriculiformis) leaves powder. The material was synthesized by mild chemical activation by using 0.1 M sodium hydroxide as an activator under room temperature stirring for 3h and isolated as a dark brown powder. The material was characterized using FTIR, FESEM, XRD, and pHzpc; and tested successfully with crystal violet and methylene blue. While FTIR confirms the presence of polyphenolic and polysaccharide moieties, FESEM reveals unprecedented circular hollow pipe-like channels decorated in a highly ordered fashion, facing pores for optimum dye uptake. The adsorption is tunable with working pH, and the maximum adsorption capacities are 67.25 and 78.55 mg g−1 for CV and MB. Both adsorption process follows Langmuir isotherm (R 2 = 0.994) and pseudo-2nd-order kinetics (R 2 = 0.999). Thermodynamic analysis verifies a spontaneous process with an endothermic interaction beside an elevated degree of randomness. About 80% of the spent material could be regenerated using 1:1 methanol/water. Analysis of industrial effluent suggests 37% removal per cycle, with an operating ceiling of 95%. To wind up, due to huge availability, porous nature, and superior adsorption capacity over other phytosorbents, NaOH-activated acacia leaves could be considered as techno-economic and potential scavengers for sustainable water treatment.

Fireproofing flammable composites using mycelium: Investigating the effect of deacetylation on the thermal stability and fire reaction properties of mycelium

This paper presents research findings on the influence of alkaline deacetylation on the thermal stability and fire reaction properties of non-pathogenic Basidiomycota fungi (mycelium) grown in molasses. The relationship between deacetylation conditions, such as incubation time and NaOH concentration, and the thermal and fire reaction properties of mycelium was investigated. The degree of deacetylation was also examined for its influence on the high-temperature thermal stability of mycelium, such as char formation. The findings indicated that the high-temperature thermal stability increased as the degree of deacetylation increased due to the conversion of chitin into chitosan as well as the presence of char-promoting hydroxyl‑terminated polysaccharide moieties. The study further investigated the influence of hollow glass microspheres on the thermal properties and microscale combustion behaviour of unmodified and deacetylated mycelium. This study provides an in-depth analysis of the thermal degradation mechanisms that govern the thermal stability and char-forming ability of unmodified and deacetylated mycelium. Additionally, the link between the thermal stability and fire reaction properties of mycelium and its deacetylated derivatives was established. Finally, the effectiveness of unmodified and deacetylated mycelium mats for fireproofing flammable glass fibre-reinforced epoxy laminates exposed to a simulated moderate-intensity fire was evaluated.

In silico approach towards polyphenols as targeting glucosamine-6-phosphate synthase for Candida albicans

Candida albicans is one of the most common species of fungus with life-threatening systemic infections and a high mortality rate. The outer cell wall layer of C. albicans is packed with mannoproteins and glycosylated polysaccharide moieties that play an essential role in the interaction with host cells and tissues. The glucosamine-6-phosphate synthase enzyme produces N-acetylglucosamine, which is a crucial chemical component of the cell wall of Candida albicans. Collectively, these components are essential to maintain the cell shape and for infection. So, its disruption can have serious effects on cell growth and morphology, resulting in cell death. Hence, it is considered a good antifungal target. In this study, we have performed an in silico approach to analyze the inhibitory potential of some polyphenols obtained from plants. Those can be considered important in targeting against the enzyme glucosamine-6-phosphate synthase (PDB-2VF5). The results of the study revealed that the binding affinity of complexes theaflavin and 3-o-malonylglucoside have significant docking scores and binding free energy followed by significant ADMET parameters that predict the drug-likeness property and toxicity of polyphenols as potential ligands. A molecular dynamic simulation was used to test the validity of the docking scores, and it showed that the complex remained stable during the period of the simulation, which ranged from 0 to 100 ns. Theaflavins and 3-o-malonylglucoside may be effective against Candida albicans using a computer-aided drug design methodology that will further enable researchers for future in vitro and in vivo studies, according to our in silico study. Communicated by Ramaswamy H. Sarma

In-vitro antimicrobial and anti-inflammatory activity of modified solvent evaporated ethanolic extract of Calocybe indica: GCMS and HPLC characterization

Recent interest in the utilization of mushroom-based bioactive compounds has increased due to their potential bioactivities and as alternatives in the reduction of high concentrations of chemical utilization. Therefore, we evaluated the physicochemical, functional, antimicrobial, and anti-inflammatory activity of the Calocybe indica. The nutritional composition of the mushroom was found to be a good source of proteins (12.48%) and fiber (6.87%). Polysaccharide and protein moiety showed both hydrophilic and hydrophobic domains and the sample showed higher water (3.01 g/g), oil binding (2.45 g/g) emulsifying (68.94), and foaming properties (59.39%). Structural characterization revealed the porous and small crystalline structure of the mushroom powder. Ethanolic extract was quantified for total phenolics and flavonoids and revealed 11.1534 ppm caffeic acid, 0.057 ppm syringic acid, 1.6385 ppm p-coumaric acid, and 0.3495 ppm rutin, respectively. Presence of ethyl tridecanoate, hexadecanoic acid ethyl ester, pentadecanoic acid ethyl ester, undecanoic acid ethyl ester, N, α, α′-trimethyl diphenethylamine, nicotinonitriles, phosphonic acid decyl-, 1-hexyl-2-nitrocyclohexane, diallyl divinylsilane, 3-phenyl-pyrrolo(2,3-β) pyrazine was confirmed during GC–MS analysis. Furthermore, the mushroom extract showed effective antimicrobial against Gram-positive (23.67 mm) and negative bacteria (20.33 mm) in terms of zone of inhibition. Significantly comparable anti-inflammatory activity was observed for mushroom extract during protein denaturation (43.72–85.69%) and membrane stabilization. In conclusion, the mushroom extract has shown good functional properties and potential bioactivity, therefore, it can be scaled up as an effective food preservative, potential anti-inflammatory, and antimicrobial agent at the industrial level.

Hydrogel for topical drug delivery based on Mimosa pudica seed mucilage: Development and characterization

A Large number of existing excipients had become common place in today's pharmaceutical formulations as additives but had certain drawbacks such as lack of biodegradability, biocompatibility, toxicity, side effects, high cost as well as environmental pollution during synthesis. As the establishment of toxicity and side effects poses a problem with synthetic excipients for the recognition as safe use from regulatory authorities, more interest is attracted towards the development of natural excipients. In the present study, attempts were made to explore mucilaginous polysaccharides isolated from seeds of Mimosa pudica as a hydro-gelling agent as mucilaginous materials obtained from plant sources could be an effective gelling agent. The isolated mucilaginous polysaccharides material was tested for its physicochemical, spectrophotometric, thermal, mechanical, biodegradation, micromeritics properties (like particle size, density, flow properties of powder) and oral toxicity study. The results reveal that isolated mucilaginous polysaccharides had slightly acidic pH, low moisture and ash content and a good swelling index with good micromeritics properties. Rheological properties showed non-Newtonian shear thinning behavior. Biodegradation study confirms its easily biodegradable nature at a reasonable rate. The FTIR and NMR spectrum showed its characteristic as polysaccharide moiety. Oral toxicity studies report of M pudica mucilaginous polysaccharide confirms it as GRAS material. Isolated mucilaginous polysaccharide exhibits hydrogel-forming ability at 1% w/w concentration. Diclofenac loaded hydrogel exhibited persistent drug release. These findings demonstrated that the Mimosa pudica mucilaginous polysaccharide could be a suitable excipient as a hydrogel-forming agent not only in drug delivery applications but also in cosmetic industries.

Insight into the mechanisms of the excellent emulsification properties of whey protein isolate-arabinoxylan conjugates

Whey protein isolate (WPI) and arabinoxylan (AX) conjugates were prepared by controlled dry-heating methods. The physicochemical, structural and emulsifying properties of WPI-AX conjugates were investigated to understand their structural and functional relationships. Overall, WPI-AX conjugates showed better emulsifying properties compared to WPI, AX, or their physical mixture. The emulsifying capacity of 0.5 wt% drying heating conjugates was comparable to that of 15 wt% commercial gum arabic (GA). The improved emulsifying properties of conjugates are mainly contributed by the enhanced steric stabilization from the bulky hydrophilic polysaccharide moiety and the increased protein solubility after covalent binding. Moreover, the impacts of molecular weights of AX on the emulsification stability of conjugated were evaluated. This study develops novel commercial emulsifiers with excellent emulsifying properties and applications at extreme conditions (e.g. 95 °C or pH 4.5, unfavorable to most commercial products), which helps to explore novel solutions for the value-added utilization of wheat brans.

The emulsification properties of alkaline-extracted polysaccharide conjugates from Apocynum venetum L. tea residues

As a non-camellia tea with a rich history in Chinese culture that spans several centuries, Apocynum venetum L. tea is a brewed beverage made from the leaves of the Apocynum venetum L. plant. Despite its long history, little research on the polysaccharides in Apocynum venetum L. leaves has been conducted. In this study, polysaccharide conjugates were extracted from Apocynum venetum L. tea residue under alkaline conditions, referred to as ATPC-A. The conjugates comprised polysaccharide moieties covalently bound to protein. Oil-in-water (O/W) emulsions were prepared from the mixtures of the ATPC-A in water and medium-chain triglycerides (MCT). The mean particle diameter (MPD) (d3,2) of the ATPC-A-stabilized emulsions containing 2.00 wt% and 3.00 wt% ATPC-A increased from 0.47 μm to 1.94 μm and 0.37 μm–1.48 μm after storage at 25.0 °C for 10 days. The zeta potentials of the emulsions were also stable around −40.00 mV. Compared to a 5.00 wt% gum arabic (GA)-stabilized emulsion, the ATPC-A-stabilized emulsion containing 1.00 wt% ATPC-A and higher featured smaller particle sizes and stabilities compared to at lower ATPC-A concentrations. Between pH 2.0 and 8.0, the MPD (d3,2) of the ATPC-A emulsions ranged from 21.05 to 5.67 μm, while the zeta potentials ranged from −25.47 to −44.88 mV. Under different concentrations of metal ions (Na+ and Ca2+), the MPD (d3,2) of the ATPC-A emulsion increased from 13.61 to 29.55 μm between 0.10 and 0.50 mol/L Na+) and from 26.60 μm to 43.72 μm between 0.01 and 0.05 mol/L Ca2+. The absolute zeta potential dropped from 35.07 to 32.53 mV (Na+) and from 33.33 mV to 17.63 mV (Ca2+). These results highlighted the utilization of polysaccharide conjugates derived from Apocynum venetum L. leaves and provided a basis for the high-value utilization of Apocynum venetum L. tea residues.

Structural characteristics of Saccharomyces cerevisiae mannoproteins: Impact of their polysaccharide part

While they have many properties of interest in enology, the structure-function relationships of mannoproteins and the part played by their polysaccharide moiety are not yet well understood. Mannoproteins (MP) extracted with β-glucanase from a laboratory yeast strain (WT), two of its mutants (Mnn2 with unbranched N-glycosylated chains and Mnn4 without mannosyl-phosphorylation), and an enological strain (Com) were purified and thoroughly characterized. The protein moiety of the four MPs had the same amino acid composition. Glycosyl-linkage and net charge analyses confirmed the expected differences in mutant strain MPs. MP-Com had the highest mannose/glucose ratio followed by MP-WT/MP-Mnn4, and MP-Mnn2 (13.5 > 5.6 ≈ 5.2 > 2.2). The molar mass dependencies of Rg, Rh, and [η], determined through HPSEC-MALLS-QELS-Viscosimetry, revealed specific conformational properties of mannoproteins related to their nature of highly branched copolymers with two branching levels. It also clearly showed structural differences between MP-Com, MP-WT/Mnn4, and MP Mnn2, and differences between two populations within the four mannoproteins.

Conjugation of α-, β-, and κ-Caseins with Propylene Glycol Alginate Using a Transacylation Reaction as Novel Emulsifiers.

Protein-polysaccharide conjugates usually have better emulsifying properties than their constituent biopolymers. In this study, casein-alginate conjugates were prepared using a transacylation reaction between different types of caseins and propylene glycol alginate (PGA) at pH 11.0. The purified conjugates had the combined molecular structure of casein and hydrolyzed PGA (alginate), according to Fourier-transform infrared spectroscopy, and α-, β-, and κ-casein-alginate conjugates had 39.17%, 37.78%, and 23.14% protein content, respectively. Emulsions were prepared with all the conjugates at a surfactant-to-oil ratio of 1:100 (w: (v), which is much lower than emulsions stabilized by Maillard-type protein-polysaccharide conjugates. The emulsions had high stability during storage and at wide pH (3.0-11.0) and ionic strength (0-450 mM) ranges. The interfacial tension of conjugate dispersions and therefore the droplet size were dependent on the protein content, not the casein type, while the polysaccharide moiety was critical to the emulsion stability. The present findings suggest a transacylation reaction can be used to prepare protein-alginate conjugates with novel emulsifying properties.

PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANT (GLYCOLIPID) FROM LACTOBACILLUS HELVITICUS M5 AND EVALUATE ITS ANTIMICROBIAL AND ANTIADHESIVE ACTIVITY

The current study was aimed to optimize, characterize and evaluate the antimicrobial and antiadhesive activity of biosurfactant(glycolipid) produce from local isolateLactobacillus helvetiusM5. The obtained results indicate that the highest emulsifying activity occurred at pH 7, Lactose (5%) the best production medium, 1% peptone as nitrogen source, maximum growth and production of biosurfactant was observed at C: N ratio (5:1) and after 120 h of incubation. Partial purified biosurfactant was characterized byfourier transform infrared spectroscopy and gas chromatography mass. FTIR results indicated aliphatic hydrocarbon chains along with a polysaccharide moiety that confirmed the glycolipid nature of the biosurfactant produced . GC analysis of glycolipid indicated the cycle aliphatic lipid nature of the structures in the biosurfactant.Antibacterial and antiadhesion activities of biosurfactant were evaluated against some pathogenic bacteria. The biosurfactant showed inhibition zones diameter ranged from (12 to 29 mm) and (15 to 31 mm) against P. aeruginosa and S. aureus respectively at concentration of glycolipid ranged from 20 to 100 mg/ml.The highest antiadhesive property was observed against S. aureus (78%) and P. aeruginosa (74.5%) at concentration 50 mg/ml of glycolipid respectively.

Effect of surfactant-induced competitive displacement of whey protein conjugated to acid- or alkali-extracted potato pectin on emulsion stability

Covalently linked protein–polysaccharide conjugate pre-adsorbed to the interface often shows more enhanced resistance to displacement induced by competitively adsorbing small molecule surfactant than the pure protein component. The objective of this study was to investigate the effect of surfactant-induced competitive displacement of whey protein–potato pectin conjugate pre-adsorbed to oil droplet surfaces on the stability of the oil-in-water emulsion. Potato pectin extracted in either an acidic or alkaline condition was conjugated to bovine milk whey protein using the Maillard reaction. The formation of covalent linkage between the protein and pectin moieties was confirmed using sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Atomic force microscopy studies of the interfacial films of the Maillard conjugates formed on flat water surfaces revealed that the alkali-extracted potato pectin provided more enhanced resistance to surfactant-induced competitive displacement from the air–water interface than the acid-extracted potato pectin. These results indicate that galactan side chains having an ability to self-assemble and form network structures had been preserved to a larger degree during the alkali-extraction. Furthermore, the alkali-extracted potato pectin prevented coalescence-induced increases in the oil droplet diameter in the oil-in-water emulsions prepared using the Maillard conjugate as the emulsifier upon the addition of up to 38.7 mM of the Tween 20 surfactant while maintaining relatively high levels of the surface load of protein (>0.8 mg/m2). The present results demonstrate that the molecular structure of the polysaccharide moiety of protein–polysaccharide conjugate has significant impacts on its resistance to competitive displacement and emulsion stability.

Sialylated Immunoglobulins for the Treatment of Immuno-Inflammatory Diseases.

Immunoglobulins are the potent effector proteins of the humoral immune response. In the course of evolution, immunoglobulins have formed extremely diverse types of molecular structures with antigen-recognizing, antigen-binding, and effector functions embedded in a single molecule. Polysaccharide moiety of immunoglobulins plays the essential role in immunoglobulin functioning. There is growing evidence that the carbohydrate composition of immunoglobulin-linked glycans, and especially their terminal sialic acid residues, provide a key effect on the effector functions of immunoglobulins. Possibly, sialylation of Fc glycan is a common mechanism of IgG anti-inflammatory action in vivo. Thus, the post-translational modification (glycosylation) of immunoglobulins opens up significant possibilities in the diagnosis of both immunological and inflammatory disorders and in their therapies. This review is focused on the analysis of glycosylation of immunoglobulins, which can be a promising addition to improve existing strategies for the diagnosis and treatment of various immuno-inflammatory diseases.

Synthesis, characterization and application of glucosyloxyethyl acrylate graft chitosan against pipeline steel corrosion

In this study, glucosyloxyethyl acrylate graft chitosan (GA-CHS) was synthesized by reacting chitosan with glucosyloxyethyl acrylate, previously prepared via catalyzed α-d-glucopyranoside transesterification with excess 2-hydroxyethyl acrylate. This graft GA-CHS copolymer was utilized as a corrosion inhibitor for API 5 L X70 pipeline steel in chloride-enriched (1 M HCl) acid solution. GA-CHS adsorption on the steel surface led to significant inhibition of chloride-induced corrosion compare to unmodified chitosan (CHS). In the presence of GA-CHS, corrosion inhibition increased with its concentration due to the formation of compact protective films; compositional and morphological evidences have been provided. Without this graft copolymer in corrosive electrolytes, the steel coupons corroded due to unrestricted chloride-induced attacks, leading to severe surfaces pits. The linear GA-CHS polysaccharide moiety could have been a contributing factor and the greater edge over unmodified chitosan, regarding enhanced corrosion inhibition as well as its hydroxyl/amino chemical groups in combination with its acrylate functionality.