Abstract
The current study was aimed to optimize, characterize and evaluate the antimicrobial and antiadhesive activity of biosurfactant(glycolipid) produce from local isolateLactobacillus helvetiusM5. The obtained results indicate that the highest emulsifying activity occurred at pH 7, Lactose (5%) the best production medium, 1% peptone as nitrogen source, maximum growth and production of biosurfactant was observed at C: N ratio (5:1) and after 120 h of incubation. Partial purified biosurfactant was characterized byfourier transform infrared spectroscopy and gas chromatography mass. FTIR results indicated aliphatic hydrocarbon chains along with a polysaccharide moiety that confirmed the glycolipid nature of the biosurfactant produced . GC analysis of glycolipid indicated the cycle aliphatic lipid nature of the structures in the biosurfactant.Antibacterial and antiadhesion activities of biosurfactant were evaluated against some pathogenic bacteria. The biosurfactant showed inhibition zones diameter ranged from (12 to 29 mm) and (15 to 31 mm) against P. aeruginosa and S. aureus respectively at concentration of glycolipid ranged from 20 to 100 mg/ml.The highest antiadhesive property was observed against S. aureus (78%) and P. aeruginosa (74.5%) at concentration 50 mg/ml of glycolipid respectively.
Highlights
Amphiphilic molecules with proven surface properties and emulsifying properties are biosurfactants
The use of cheap substrates such as agro-industrial waste, the optimisation of medium and crop conditions, the development of efficient recovery processes and the production of microorganisms will contribute to making their development more economically attractive by developing cheaper and more effective processes (25).A number of studies have documented the ability of lactobacilli as producers of biosurfactants (29).Biosurfactants formed on silicone rubber and other biomedical instruments by LAB(Lactic acid bacteria) damaged biofilm formations (11)
The current study was aimed to production, and characterization of glycolipid produced by Lactobacillus sp.and evaluated its antibacterial activity
Summary
Amphiphilic molecules with proven surface properties and emulsifying properties are biosurfactants. The flasks were inoculated with 2% (1x108 cell/ml) of Lactobacillus inoculum and incubated in shaker (120 rpm) at 37°C for 120hrs the samples were taken from each flask for the determination of biomass and biosurfactant production. The flasks were inoculated with 2%Lactobacillus and incubated in different incubation periods include (24, 48, 54, 72, 78, 96, 102, 120 ,126 ) using shaker incubator , the sample were taken from each flask for estimation of biomass and biosurfactant production. The stain associated with the adherent pathogens was solubilized with 100 μl of 33% glacial acetic acid for each well and the optical density readings of individual wells were recorded at 595 nm using micro Elisa auto reader (Model 680, Bio-Rad) This was followed by the preparation of Bacterial suspension with no biosurfactant, as control.
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