This article use the Sulphide Isobutene (T321), Five Sufides Dialkyl(RC2540) and Star of Phosphorus(P110) as the additives,Neopentyl Polyol Ester(NPE) and mineral oil N32 as base oil. Compound above additives and base oil for the four levels. A sample: adding 4% T321 additive in NPE. B sample: adding 4% T321 additive in N32. C sample: adding 4% RC2540 additive in NPE. D sample: adding RC2540, T321 and P110 additives in NPE (all is mass fraction). The oral acute toxicity test, eye mucous stimulation test, skin hypersensitive test, soaking tail toxicity tests were conducted in above samples. The test results show that. The mineral oil, it’s not only toxic then synthetic oil but also has a poor lubricating ability compare with the same percent additive in synthetic oil. In oral acute toxicity test, eye mucous stimulation test, skin hypersensitive test, soaking tail toxicity tests, Toxic reaction of mineral N32+4%wt Sulphide Isobutene (T321) obviously from other oil samples.