BackgroundRhD variants are categorized into partial D, weak D, and DEL. The detection of DEL can only be achieved through the adsorption and elution method or by using molecular techniques. Here, we report a case of DEL phenotypes associated with a novel allele in a Chinese individual. Study Design and MethodsWe used serological methods such as saline, indirect anti-human globulin and adsorption-elution. The RHD genotype was determined by the PCR-sequence specific primer (PCR-SSP) method as well as the Sanger dideoxy sequencing. ResultsRBCs of the sample were found to be DEL phenotype by serological testing, with negative reaction in the saline and indirect anti-human globulin test while positive reaction by the absorption–elution method. The genotyping results revealed a hemizygous (RHDc.1127T>G/RHD-). The novel allele sequence has been submitted to GenBank (Accession number: OR608456). ConclusionOur study demonstrates a case of a Chinese individual with DEL phenotype caused by a novel allele RHD c.1127T>G. It expands the database of the DEL variant.