Association of Hematological and Biochemical Parameters and HLA-DRB1 Alleles With Anti-cyclic Citrullinated Peptide Autoantibodies in Sudanese Rheumatic Patients.

Abstract

Introduction Anti-citrullinated protein/peptide antibodies (ACPA) are crucial for the diagnosis and prognosis of rheumatoid arthritis (RA) and are associated with class II HLA-DRB1 alleles. The study's goal was to determine howDRB1alleles and hematological and biochemical parameters affect ACPA production in RA patients from Sudan. Methods The study analyzed the hematological and biochemical parameters and the frequency ofHLA-DRB1alleles in 120 RA patients and 100 controls. Automated analyzers, ELISA, the latex agglutination test, and the Westergren method were utilized for hematological and biochemical testing. HLA class II alleles were genotyped using polymerase chain reaction-sequence-specific primers (PCR-SSP). The student's t-test and the chi-square (Χ2) test were employed to identify significant alterations between the examined parameters and allele frequencies. Results A total of 51.7% of 120 RA patients tested positive for ACPA (ACPA+). Among those patients, theDRB1*04and*10alleles were significantly more prevalent (22.2% vs. 8.9%, P = 0.048 and 23.8% vs. 8.9%, P = 0.030, respectively). RA patients had significantly higher counts of platelet count test (PLT; P= 0.011), lymphocytes (LY; P =0.000), neutrophils (NE; P= 0.025), monocytes (MO; P= 0.000), eosinophils (EO; P= 0.000), neutrophil-to-lymphocyte ratio (NLR; P= 0.006), C-reactive protein (CRP; P= 0.000), and erythrocyte sedimentation rate (ESR; P= 0.000) than controls. Patients also showed low counts of red blood cells (RBC; P= 0.003), hemoglobin (Hb; P= 0.024), mean platelet volume (MPV; P= 0.000), and basophils (BA; P= 0.048). ACPA+RA patients had elevated white blood cells (WBC; P= 0.046), PLT (P= 0.029), and low mean corpuscular hemoglobin concentration (MCHC; P =0.022). The hematological and biochemical parameters of ACPA+RA patients with theDRB1*04or*10alleles did not differ significantly. Conclusions We found significant differences in hematological and biochemical parameters between RA patients and controls that had nothing to do with ACPA positivity or the frequency ofDRB1*04or*10alleles.