Twenty-seven castrated male adult rats of Wistar strain were devided into three experimental groups: The 1st group is non-treated with post-puberal gonadectomy as the control, the 2nd is repeatedly given testosterone propionate (T. P.) 0.05mg/day immediately after the operation, and the 3rd is repeatedly given T. P. 0.5mg/day in the same way. All experimental animals with or without the prolonged administration of the hormones were decapitated respectively 2, 6 and 10 weeks after the castration. The cytological observations obtained in the cells of adenohypohysis were as follows:Two weeks after the castration, most of β-cells became larger and spherical (gonadotroph), but slender polygonal β-cells (thyrotroph) frequently occurring in intact animals were reduced in number by the removal. Two kinds of β-cells were together strongly stained in general with periodic acid SCHIFF's reaction (PAS). The former is scattered at the whole area of adenohypophysis, and especially accumulated at the vicinity of pars intermedia (sex zone) and the center of the anterior lobe. The signet ring possibly identified to the negative shadow of GOIGI apparatus increased in number and diameter in gonadotrophs. Within 6 weeks, there appeared a great majority of swelling gonadotrophs containing a large size of signetring. Huge colloid substance occupping the cell-body was often recognized in some of large gonadotrophs. Such a kind of β-cell including many tiny vacuoles looks to be rather akin to a degenerating cell. Within the lapse of 10 weeks the whole area of adenohypophysis was full of large spherical gonadotrophs in which expanded signet-ring is usually situated. They may correspond to so-called castration cell of MAXIMOW. α-cells atrophied in 2 weeks, but conversely enlarged in 6 weeks and recovered to the normal level in 10 weeks. The steps in transformation of γ-cells into β-cells were detectable with facility all through the periods following the castration.In spite of the administratin of T. P. 0.05mg/day to the castrated rats, gonadotrophs were not altered in their frequency in 2 weeks; most of them constantly enclosed signet-ring and were stained with PAS, resembling the castration cells, while thyrotrophs stained strongly with PAS began to appear abundantly. By the prolonged administration for 6 weeks, gnoadotrophs diminished apparently in number and fell into a hyperchromatic degeneration, leaving the atrophic signet-ring within the cell-body, they were, therefore, no longer entitled to the castration cells. Further the number of gonadotrophs was reduced profoundly in 10 weeks, whose signet-ring showed the reduction in diameter, despite their reacting ability with PAS was not so much alleviative. The alterations in α-cells induced by the castration resulted in the restoration by the hormone therapy. The changes in α-cells are equivalent in general to those in castrated rats.In rats with the persistent injection of 0.5mg/day T. P., most of β-cells atrophied exceedingly even in 2 weeks; they were strongly stained with PAS and provided the condensed homogeneous cytoplasm sometimes containing a small size of signet-ring. Thus the injection of higher dose of hormone made not only the atrophic pattern, but also remarkable reduction in number of β-cells. Evidences were present that gonadotrophs produced an improvement of pathological hyperactivity induced by the castration, and moreover attained to status of hypoactivity. α-cells increased in number and were recoved from the diminution following the castration. Within 6 weeks all of β-cells fell into outstanding atrophy and have slender polygonal contour without an exception; large spherical gonadotrophs completely disappeared in remaining a few withering thyrotroph at the center of the adenohypophysis. However, atrophic changes were much or less alleviated in 10 weeks