Polyfloral Honey Research Articles

Honey Enriched with Additives Alleviates Behavioral, Oxidative Stress, and Brain Alterations Induced by Heavy Metals and Imidacloprid in Zebrafish.

Environmental concerns have consistently been a focal point for the scientific community. Pollution is a critical ecological issue that poses significant threats to human health and agricultural production. Contamination with heavy metals and pesticides is a considerable concern, a threat to the environment, and warrants special attention. In this study, we investigated the significant issues arising from sub-chronic exposure to imidacloprid (IMI), mercury (Hg), and cadmium (Cd), either alone or in combination, using zebrafish (Danio rerio) as an animal model. Additionally, we assessed the potential protective effects of polyfloral honey enriched with natural ingredients, also called honey formulation (HF), against the combined sub-chronic toxic effects of the three contaminants. The effects of IMI (0.5 mg·L-1), Hg (15 μg·L-1), and Cd (5 μg·L-1), both individually and in combination with HF (500 mg·L-1), on zebrafish were evaluated by quantifying acetylcholinesterase (AChE) activity, lipid peroxidation (MDA), various antioxidant enzyme activities like superoxide dismutase and glutathione peroxidase (SOD and GPx), 2D locomotor activity, social behavior, histological and immunohistochemical factors, and changes in body element concentrations. Our findings revealed that all concentrations of pollutants may disrupt social behavior, diminish swimming performances (measured by total distance traveled, inactivity, and swimming speed), and elevate oxidative stress (OS) biomarkers of SOD, GPx, and MDA in zebrafish over the 21-day administration period. Fish exposed to IMI and Hg + Cd + IMI displayed severe lesions and increased GFAP (Glial fibrillary acidic protein) and S100B (S100 calcium-binding protein B) protein expression in the optic tectum and cerebellum, conclusively indicating astrocyte activation and neurotoxic effects. Furthermore, PCNA (Proliferating cell nuclear antigen) staining revealed reduced cell proliferation in the IMI-exposed group, contrasting with intensified proliferation in the Hg + Cd group. The nervous system exhibited significant damage across all studied concentrations, confirming the observed behavioral changes. Moreover, HF supplementation significantly mitigated the toxicity induced by contaminants and reduced OS. Therefore, the exposure to chemical mixtures offers a more complete picture of adverse impacts on aquatic ecosystems and the supplementation with bioactive compounds can help to reduce the toxicity induced by exposure to environmental pollutants.

Evaluation of the Antioxidant Activity and Phenolic Composition of Different Monofloral and Polyfloral Brazilian Honey Extracts.

This study aimed to evaluate the chemical composition and antioxidant activity of phenolic extracts from monofloral and polyfloral honey samples obtained from different Brazilian regions. The chemical composition (total content of phenolic compounds and flavonoids) of extracts were measured by using colorimetric assays and analyzed by high performance liquid chromatographic (HPLC-DAD). The antioxidant activity was evaluated by chemical and biochemical assays (reducing power assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH⋅) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS⋅+) scavenger assays. It was also investigated the ability of extracts in attenuate lipid peroxidation induced by Fe2+ in phospholipids. The obtained results clearly demonstrated that the botanical origin and geographical region of honey collection influenced the chemical composition and antioxidant activity of extracts. Furthermore, the samples were constituted by phenolic acids and flavonoids, which p-coumaric acid was predominant among the compounds identified. All samples were able to scavenge free radicals and inhibit lipid peroxidation, and good correlations were obtained between the flavonoid content and honey color. In conclusion, the obtained extracts were constituted by antioxidant compounds, which reinforce the usage of honey in human diets, and demonstrated that the region of honey collection strong influenced in the chemical composition and, consequently, its biological effect.

Functional Cyperus esculentus L. Cookies Enriched with the Probiotic Strain Lacticaseibacillus rhamnosus SL42.

This study presents for the first time functional cookies for diabetics made with 100% organic Cyperus esculentus L. flour, either plain or enhanced with 5% polyfloral honey syrup containing the probiotic strain Lacticaseibacillus rhamnosus SL42. The flour's chemical composition and rheological and functional properties were analyzed, and 33 diabetic and semi-naive panelists assessed the cookies' sensory properties. MRS-cys agar cultures and SEM analysis evaluated SL42 survival and adhesion capacity over 21 days at 25 °C. Results showed that the flour and its extracts are rich in polyphenols and flavonoids, indicating strong antioxidant and antibacterial properties. Both cookie types met international standards when compared to commercial cookies and had similar physical properties. Sensory evaluation on day 1 revealed higher quality indicators for honey syrup-enriched cookies, but after 15 days, control cookies were preferred. The CIE LAB analysis confirmed the dietetic flour's typical dark color, with honey syrup-enriched cookies being darker. Despite textural differences, both cookies maintained detectable crispness over storage. Honey syrup-enriched cookies effectively carried L. rhamnosus SL42, remaining viable at 6.43 Log CFU per cookie after 21 days and adhering to the cookie's surface, as confirmed by SEM analysis. Further research is recommended to better understand the therapeutic value of these cookies.

Differential Digestive Stability of Food-Derived microRNAs: The Case of miR-30c-5p and miR-92a-3p in Polyfloral Honey.

Dietary microRNAs (miRs) represent a new area in food science. Although they have been found in many foods, including honey, more research is needed about their stability and fate during digestion. Hence, this study aimed to analyze the digestive stability of two selected miRs in honey. We extracted miR-92a-3p and miR-30c-5p from pasteurized and unpasteurized forms of polyfloral honey using two different methods and kits: a column-based manual method and a phenol-free semi-automated magnetic-bead-based method. The latter option was used for the subsequent analysis of samples according to the INFOGEST static in vitro digestion protocol. Also, the honey samples were examined for exosome-like particles using dynamic light scattering. Although the expression levels of both miRs were significantly lower following intestinal digestion, we found a difference in the resilience of the miRs to gastrointestinal conditions, with miR-30c-5p being relatively stable compared to miR-92a-3p following digestion, regardless of the honey's pasteurization treatment. Moreover, there was marked heterogeneity in the extracellular vesicle profile of the pasteurized sample. We identified the presence of two broadly conserved miRs in honey: miR-92a-3p and miR-30c-5p. Despite honey exhibiting high digestibility, miR-92a-3p was less resilient than miR-30c-5p, demonstrating considerable resistance under gastrointestinal conditions. Although further research is needed, the results obtained from this study may represent a starting point for utilizing honey as a source of exogenous miRNAs for preventive strategies and more "natural" treatments.

Composition of Proteins Associated with Red Clover (Trifolium pratense) and the Microbiota Identified in Honey.

The nutritional composition of honey is determined by environmental conditions, and botanical and geographical origin. In addition to carbohydrates, honey also contain pollen grains, proteins, free amino acids, and minerals. Although the content of proteins in honey is low, they are an important component that confirms the authenticity and quality of honey; therefore, they became a popular study object. The aim of the study was to evaluate protein content and composition of monofloral red clover and rapeseed honey collected from five different districts of Lithuania. Forty-eight proteins were identified in five different origin honey samples by liquid chromatography. The number of red clover proteins identified in individual honey samples in monofloral red clover honey C3 was 39 in polyfloral honey S22-36, while in monofloral rapeseed honey S5, S15, and S23 there was 33, 32, and 40 respectively. Aphids' proteins and lactic acid bacteria were identified in all honey samples tested. The linear relationship and the strongest correlation coefficient (r = 0.97) were determined between the content of Apilactobacillus kunkeei and Apilactobacillus apinorum, as well as between the number of faba bean (Vicia faba) pollen and lactic acid bacteria (r = 0.943). The data show a strong correlation coefficient between the amount of lactic acid and aphid protein number (r = 0.693). More studies are needed to evaluate the relationship between the pollination efficiency of red clover by bees and the multiplicity of red clover proteins in honey protein, as well as microbiota diversity and the influence of nature or plant diversity on the occurrence of microbiota in honey.

IDENTIFICATION OF THE BOTANICAL ORIGIN AND DETERMINATION OF PHYSICOCHEMICAL PARAMETERS OF HONEY SAMPLES COLLECTED FROM THE SARY-CHELEK BIOSPHERE RESERVE OF KYRGYZSTAN

Sary-Chelek Biosphere Reserve is one of the richest rare flora and animal reserves in Kyrgyzstan. The natural environment of the Sary-Chelek Biosphere Reserve is exceptionally favourable to high-quality honey production. Botanical authenticity is the main factor influencing honey prices, as it has a direct impact on the quality of honey. Producing monofloral honey in a natural landscape is challenging because, throughout the beekeeping season, multiple melliferous plants bloom at the same time. The article describes for the first time the botanical origin and physicochemical characteristics of honey collected in this biosphere reserve and also determines the influence of the dominant honey plant on organoleptic, physicochemical, and food safety indicators. Pollen grains of 50 taxa of nectar sources and about 10 taxa of pollen sources were identified. There were unique plant pollens in the samples; nine of the samples were monofloral honey—three of sage (Salvia officinalis L.), four of thyme (Thymus vulgaris L.), and two of eremurus (Eremurus fuscus O.Fedtsch), and eight samples were polyfloral honey. High diastase activity was found in samples of thyme honey (41.1 ± 2.9 Gothe) and in samples of sage honey (31.3 ± 2.2 Gothe). The physicochemical and food safety parameters of the honey samples comply with the established norms and international standards.

Impact of floral and geographical origins on honey quality parameters in Saudi Arabian regions

This article examined the effect of geographical (different climate conditions) and floral origins on some quality parameters of honey including the activity of diastase enzyme. Moreover, some non-quality parameters were investigated such as the pH, fructose, glucose, ratio of fructose/glucose and invertase. The honey samples were collected from Asir (cold climate) and Jazan (hot climate) regions at the southwestern part of Saudi Arabia. The geographical origin significantly affected the mean value moisture of the Acacia honey (p-value = 0.02), conductivity of the polyfloral honey (p-value = 0.03), sucrose of the Acacia honey (p-value = 0.02), diastase activity of the Acacia (p-value = 0.001), Ziziphus (p-value = 0.046) and polyfloral honey (p-value ≤ 0.001), fructose of the Acacia honey (p-value = 0.01), glucose of the Ziziphus honey (p-value = 0.03), fructose/ glucose ratio of the Ziziphus honey (p-value = 0.035), and invertase activity of the polyfloral honey (p-value ≤ 0.001). Regarding the effect of the floral origin of the honey from Asir region, the sucrose percentage of the Acacia honey was significantly more than that of the polyfloral honey (p- value = 0.003), the diastase activity of the Acacia honey was significantly more than its activity in the Ziziphus honey (p- value = 0.044), glucose percentage of the Ziziphus honey was significantly more the glucose percentage of the Acacia honey (p-value = 0.009) and the fructose/ glucose ratio of the Ziziphus honey was significantly more than that of the Acacia and polyforal honeys (p-value = 0.011 and p-value = 0.045, respectively). Concerning the significant effects of the floral origin on the quality parameters of the honey samples from Jazan region, the moisture of the Ziziphus honey was significantly increased when compared to the moisture of the Acacia honey (p-value = 0.038), the acidity of the polfloral honey was significantly more than the acidity of the Acacia honey (p-value = 0.049), the sum of fructose and glucose of the polyfloral honey was significantly increased compared to that of the Acacia honey (p-value = 0.015), the pH of the Ziziphus hiney was significantly more than the pH of the polyfloral honey (0.011) and the fructose of the polfloral honey was significantly more than that of the Acacia honey (p-value = 0.031). The effect of the geographical origin of the honey samples on their quality parameters depends on their floral origin and the effect of their floral origin differs according to their geographical origin. This article suggests considering collectively the geographical and floral origins effect when developing honey standards. However, the Codex standards for honey started considering this issue when it changed the standard concentration of HMF in honey from not more than 80–40 mg/Kg for honeys from cold climate and 80 mg/Kg for honeys from hot climates.

Improving honey identification: Stable isotope ratios variability of mono and polyfloral honeys from the citrus growing area of Salto/Concordia and from Uruguayan coastal areas

Concordia in Argentina and Salto in Uruguay are two neighbouring regions famous for the production of a precious citrus honey, whose valorisation passes through the ability to guarantee its geographical origin. In this study the influence of the different botanical origins (monofloral and polyfloral) within the same region of origin on the different stable isotope ratios was evaluated. Moreover, the effectiveness of the stable isotope ratio parameters δ13Choney, δ13Cprotein, δ15Nprotein, δ34Sprotein, δ2Hprotein and δ18Oprotein to discriminate between honeys produced in the internal border regions between Argentina and Uruguay (namely, Concordia and Salto) and honeys sampled in the two different Uruguayan coastal regions (Canelones and Maldonado) was tested on 82 monofloral and polyfloral honey samples. The results show that the different stable isotope ratios, except that of nitrogen, are not significantly influenced by the botanical origin of the samples. The sulphur isotopic ratio of proteins and carbon of honey are the most significant parameters for discriminating the geographical origin of the honeys considered. Applied a Principal Component Analysis, the first two factors overall explain 63.5% of the total variance, while the Discriminant Analysis provided optimal discrimination between the three origins, reaching a minimum of 96.7% correct reclassification.

Application of UV–vis spectrophotometry and chemometrics to investigate adulteration by glucose syrup in Brazilian polyfloral honey

The quality control of food in an easy, fast, and low-cost way has been increasingly sought by industry, and methods based on spectroscopy have stood out in recent years. Honey is among the most adulterated foods worldwide, and fraud detection is difficult because it is a chemically complex matrix. In this sense, this study aimed to develop a simple, fast, and cheap method based on UV–vis spectrophotometry coupled to chemometrics for discriminating pure Brazilian polyfloral honey from adulterated ones. For that, 139 real samples of polyfloral honey produced in southern Brazil (Santa Catarina state) were analyzed by UV–vis scanning spectrophotometry (λ 200–800 nm), and a set of 42 samples (i.e., 30%) were intentionally adulterated with glucose syrup (10–60%, w/w). Three wavelength windows were shown to be relevant in the UV–vis spectra (e.g., 260–500 nm, 260–360 nm, and 280–300 nm), and these data sets were further analyzed by principal component analysis (PCA). Pure and adulterated honey samples were better discriminated by computing the 280–300 nm spectral window data (PC1 and PC2 = 98% total variance of the data set). To validate the method, 59 samples produced in southeastern Brazil (São Paulo state) were included in the descriptive model, which allowed identifying pure and adulterated honey again (PC1 + PC2 = 97% of the data set’s total variance). Thus, these findings support that UV–vis coupled with PCA proved to be a simple and efficient method to discriminate pure honey from adulterated ones, providing a fast and inexpensive protocol to monitor honey quality.

Multi-Element Profile Characterization of Monofloral and Polyfloral Honey from Latvia.

Honey is of scientific interest mainly due to its health-promoting and antibacterial properties, which are also associated with its floral origins. However, the methods for confirming honey floral origins are quite limited and require improvements. One method suggested in the search for a multi-method approach to evaluating the floral origins of Latvian honey is inductively coupled plasma mass spectrometry (ICP-MS). This study investigated the multi-element profile of 83 honey samples of well-specified floral origins. The main findings included using Ba, Ca, Cs, Fe, and Rb as indicator elements for heather honey. The chemometric evaluation supported the use of ICP-MS for distinguishing heather honey from other types of honey. The Latvian polyfloral honey multi-element profile was defined and compared to honey samples with other geographical origins. Additionally, the multi-element profiles of buckwheat, clover, and polyfloral honey proteins were investigated to clarify whether the majority of elements were bound with proteins or not. Preliminary results indicated that Ca, K, Mg, Mn, Na, and Sr were mainly found in non-protein-bound forms, while the majority of Al, Cu, Ni, and Zn were in the form of large chemical structures (>10 kDa).

Determination and assessment of some physicochemical parameters of Bulgaria sunflower and polyfloral honey

Honeybee products are highly valued food products in the nutrition and medicine.This work aims to determine physicochemical parameters of polyfloral and sunflower honey, which are produced in Bulgaria. Twelve sunflower and 17 polyfloral honey samples were used in this study. The parameters color, water content, electrical conductivity, specific optical rotation, pH, proline, invertase, diastase, hydroxymethylfurfural (HMF) were analyzed. Bulgarian sunflower and polyfloral honeys were characterized by main physicochemical parameters and compared to products from other countries. The color correlates with the electrical conductivity and diastase correlates with invertase in the sunflower honey. The results of the present study indicated that Bulgarian sunflower and polyfloral honey are known for its good quality since the criteria that were closely correlated with the honey’s quality were measured.

Assessing the quality of bee honey on the basis of melissopalynology as well as chemical analysis.

Melissopalynological and chemical analysis of honey provide us useful and valuable information about the botanical and geographical origin of honey. The data in question is very important for authentication as well as for testing the quality of honey, so this is considered the main method in honey regulation here, we have used chemical analysis and melissopalynology to evaluate different honey samples from two main Iranian hubs of honey. Sampling was carried out on two important poles in Iran's honey production, the central Alborz region, and the mountainous Zagros ecosystems in the years 2020 to 2021. Therefore, 52 samples from Alborz (Northern Iran), as well as 42 samples from the Zagrosian ecosystems (western Iran) belonging to different ecological habitats, were collected. In addition, samples were taken at 7 altitudes from 0 to 3500 m a.s.l. Furthermore, in this study, various chemical analyses such as the effect of antioxidant activity, the amount of total phenolic content, pH, and moisture content of honey samples were evaluated. Our results showed that all honey samples were classified as polyfloral honey. Based on our findings, 57 honey samples (61%) contained the standard amount of pollen. A total of 42 plant families and 55 genera were identified in the studied samples, with the highest presence of Asteraceae, Fabaceae, Rosaceae, Apocynaceae, and Apiaceae. Finally, an antioxidant activity ratio of 19% to 98%, total phenolic content from 0.08 to 0.51 ppm, pH from 1.90 to 5.21, and moisture content from 13% to 18.40%.

Characterization of Brazilian floral honey produced in the states of Santa Catarina and São Paulo using quantitative nuclear magnetic resonance (qNMR) spectroscopy

This study applied qNMR spectroscopy and chemometrics to metabolically profile polyfloral honey samples collected in southeastern (São Paulo - SP) and southern (Santa Catarina - SC) Brazil, over the 2019–2020 and 2020–2021 harvest seasons. Both 1D and 2D NMR experiments were carried out to identify and quantify metabolites, followed by descriptive statistical analysis, heatmap, and principal component analysis (PCA), considering geographic and botanical origins. Twenty-one metabolites were detected, encompassing carbohydrates, amino acids, organic acids, ketones, alcohols, esters, and alkaloids. The heatmap and the PCA allowed for distinguishing the geographical and botanical origin. Regarding the botanical origin, three clusters were detected, i.e., Hovenia dulcis (SC), Citrus spp. (SP), and Eucalyptus spp. (SC and SP). The analytical approach proved to be effective for determining the geographic and botanical origins of Brazilian polyfloral honey, even though the country has a huge floral diversity and, accordingly, different types of honey.

Exploring Consumers' Preferences and Attitudes to Honey: Generation Approach in Slovakia.

Honey is popular among consumers for its composition and healing properties. The aim of the paper is to study the differences in honey preferences across various age generations in Slovakia. The study is based on primary data obtained by conducting an online questionnaire survey on a sample of 1850 Slovak consumers of honey in 2022. Multiple correspondence analyses and non-parametric tests were applied to study the differences in preferences across selected age cohorts (Generation Z, Generation Y, Generation X and Silver Generation). The results show that Silver Generation tends to consume honey due to its nutritional values and prefers to consume monofloral honey of a dark colour, while Generation Z does not use honey in cosmetics or consume it due to its nutritional values and are inclined to prefer polyfloral honey. The utilisation of honey in cosmetics was associated mostly with Generation X. Younger consumers (Generation Z and Generation Y) have a very low awareness of creamed honey and honey with additions in comparison to Silver Generation or Generation X. In addition, the results reveal that propolis, royal jelly and bee pollen were the most attractive additions for honey across all age cohorts in Slovakia, while spirulina and chilli were the least attractive additions.

Botanical Origin, Physicochemical Composition and Antioxidant Content of Comb, Crushed and Processed Honey Collected from Burie-Ethiopia

Honey quality and authenticity continue to be important factors in honey consumption and marketing. The major concern with honey quality is to keep its natural properties and compositions. This research work is aimed at investigating the botanical origin, physicochemical, antioxidant, rheological, and sensorial quality characteristics of comb, crushed, and processed honey. Accordingly, thirty samples were collected from Burie district of Ethiopia. Based on Melissopalynology, botanical origin was determined. Physicochemical properties were determined based on the standard methods of the International Honey Commission (IHC) and the Association of Official Analytical Chemists (AOAC). Antioxidant properties and diastase activity were determined using UV-visible spectroscopy. All the samples were poly floral honey. Comb honey was found to have moisture (17.98±0.72), sucrose (2.46±0.77), diastase activity (11.78±1.5 DN), total phenol content (TPC) (120.8±47.4 GAE/100gm), total flavonoid content (TFC) (53.7±24 CEQ/100g), 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition (30.9±14.9%), IC50 (54±5.72 mg/ml), hydrogen peroxide (H2O2) scavenging (57±16.06%). It was determined that crushed honey contains moisture (19.43±0.40), sucrose (4.21±0.76), diastase activity (9.28±0.25), TPC (84.3±51.86), TFC (29.4±61), DPPH inhibition (33.45±5.07), inhibitory concentration (IC50) (80±6.25), H2O2 scavenging (52.99±15.9). Analysis of processed honey indicated that moisture (23.76±0.78), sucrose (6.12±0.6), diastase activity (7.76±0.71), TPC (66.7±30.59), TFC (29.47±11.22), DPPH inhibition (33.98±1.84), IC50 (120±8.25), H2O2 scavenging (56.61±4.86). Correlation analysis indicated that moisture associate to sucrose content (r=0.88), diastase activity related to sucrose (r= -0.86), TPC correlates with H2O2 scavenging activity (r=0.82). Honey samples were found to be a Newtonian fluid. The sensory quality of comb honey is preferable. The result revealed that comb honey adequately satisfies the requirements of national and international standards.

Physicochemical properties and antioxidant activities of thermally treated poly-floral honey from Chire woreda, Sidamo regional State, Ethiopia

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Physicochemical Properties, Antioxidative and Antibacterial Activities of the Persian Medicine-Based Maolasal Honey: An Experimental Study

Background: Maolasal is a honey-induced product which may lead to several beneficial effects from the Persian Medicine (PM) point of view. Heating process as a major step of Maolasal preparation may decrease the honey quality and deteriorate thermal treatment indicators of honey. Methods: This study was conducted on four honey samples (from different floral sources). Maolasal preparation process was done according to one of the most important PM references “Gharabadin Kabir”. Thermal treatment indices, antibacterial and antioxidative activities of products were measured. Results: The 5-Hydroxymethylfurfural (HMF) concentrations of PM-based Maolasal samples of polyfloral, monofloral, and sugar honey were higher than 40 mg/kg; while PM-based Maolasal sample of monofloral honey had acceptable HMF concentration (38.9 mg/kg). Proline content of all samples decreased thorough Maolasal preparation and simple heating processes in all samples and these reductions were greater in monofloral honey and sugar honey Maolasal samples. Maolasal preparation processes decreased diastase activity of all samples. Although Maolasal preparation process led to sucrose content reduction in the three Maolasal samples, sucrose content of Maolasal sample of monofloral honey was higher than its raw sample. Maolasal preparation process increased total phenolic content of all samples. Finally, Maolasal preparation process led to an increase in antibacterial and antioxidant activities of all the samples. Conclusion: Maolasal samples reduced diastasis activity and proline content and increased HMF concentration. Maolasal preparation process may lead to an increase in antibacterial and antioxidative activities of honey which may be helpful for special preventive/therapeutic medicinal objectives.

Assessment of the botanical origin of Bulgarian honey samples using melissopalynological, DNA barcoding and NMR analyses

Polyfloral honey samples from Bulgaria were subject to parallel analyses of their botanical origin and composition using traditional melissopalynology, DNA barcoding based on the plastid rbcL gene and NMR analysis. The obtained datasets were compared with each other to evaluate the information capacity of the applied experimental methods. The results from the melissopalynological and DNA-barcoding studies demonstrated a significantly higher resolution of the latter, revealing the presence of pollen from a total of 17 plant families, 21 plant genera and 5 plant species in comparison to pollen from only 7 plant families, 3 plant genera and 4 plant species identified by melissopalynology. The higher resolution of DNA barcoding allows a more detailed characterisation of the diet and foraging preferences of honey bees, including foraging on plant species growing in lower abundance in the area. The comparison of the quantitative data on floral honey composition for several plant genera and species reveals significant differences between the relative abundance of the pollen grains estimated by melissopalynological analysis and the relative abundance of rbcL clones in rbcL libraries determined after DNA barcoding. All three applied methods confirm the polyfloral botanical origin of the analysed samples and support routine NMR use for the assessment of the floral origin of honey.

THE INFLUENCE OF PREVENTING CRYSTALLIZATION TREATMENT ON THE SELECTED QUALITY PARAMETERS OF HONEY

Background. Honey is a popular food product because of its taste, nutritional and health values. It is valued for its rich composition. During storage, honey changes its consistency from liquid to solid. The length of this process varies depending on the botanical origin of honey. The goal of the study was to determine the impact of thermal treatment on the selected qualitative features of honey and to extend the period during which honey remains liquid. The research material was non-purified, raw polyfloral honey. To prevent quick honey crystallization, hot air, microwave radiation and ultrasonic treatment was carried out. In order to identify changes in the structure of honey, its moisture content, hydroxymethylfurfural (HMF) content, the degree of honey crystallization during storage using the measurement of the amount of backscattered light (BS) and thermal properties using differential scanning calorimetry (DSC) were deter mined. Results and conclusions. Each of these treatments was accompanied by an increase in the hydroxy methylfurfural content (HMF). However, the HMF content in heat-treated honey still meets the Council Directive 2001/110/EC criteria (< 40 mg/kg). To measure the progress of honey granulation during stora ge, the amount of backscattered light (BS) was checked. The microwave-heated honey remained in the liquid state for a long time, which was confirmed by a low amount of backscattered light. On the other hand, a common treatment preventing honey granulation, i.e. hot air, was not the most efficient method to prolong the storage time of liquid honey. Moreover, the honey heated with hot air had the highest HMF content and BS amount.

APPLICATION OF SPECTROSCOPY IN ANALYSIS OF SOME FLORAL HONEY SAMPLES COLLECTED FROM FOUR SOUTH ROMANIAN COUNTIES IN 2021

Honey is a concentrated aqueous solution of sugars, especially glucose and fructose, and minor amounts of dextrin, enzymes, volatile oils, organic acids, ethers, and minerals. Honey samples vary in quality according to various factors, such as climate diversity, geographical characteristics, floral supply period, and packaging and storage conditions, which can compromise the quality of the hive's final product. The assessment of honey quality is a major concern that has gained interest internationally because honey has always been subjected to various fraudulent practices which become more sophisticated in the present day. Consumers, beekeepers, and regulatory bodies are interested to have reliable analytical tools and information to allow the detection of adulterated honey. The UV-VIS spectroscopy and FTIR spectroscopy techniques are two analytical methods used to analyze the honey quality nowadays, with hydroxymethylfurfural being used as a standard for testing honey's freshness. The present study was conducted to assess the quality of some floral honey samples (rapeseed, acacia, and polyfloral honey) produced in Argeș, Călărași, Giurgiu, and Teleorman counties from the south of Romania. Differences between honey botanical and geographical origins were analyzed and highlighted with One-way ANOVA (p˂0.05), followed by Duncan`s Multiple Range Test. Our results indicated that honey is a valuable source of bioactive compounds.