Polyethylene Pellets Research Articles

Exploring the effects of polyethylene and polyester microplastics on biofilm formation, membrane Fouling, and microbial communities in Modified Ludzack-Ettinger-Reciprocation membrane bioreactors

Microplastics (MPs) inevitably enter wastewater treatment plants (WWTPs), yet their impacts remain poorly understood. This study investigates the effects of MPs on system performance and membrane fouling in a Modified Ludzack-Ettinger (MLE)-Reciprocation Membrane Bioreactor (rMBR), an energy-efficient alternative to conventional membrane bioreactors. Additionally, the study examines changes in microbial community induced by different types and shapes of MPs—polyethylene (PE) pellets and polyester (PES) fibers— as well as biofilm formation on MPs, using next-generation sequencing. Results revealed that transmembrane pressure (TMP) increased 2–3 times faster in the presence of PE pellets, while TMP remained stable during the PES stage, implying that MP type and shape could influence biofouling behaviors. Furthermore, enhanced nitrate removal was observed in the aerobic tank due to denitrifying biofilm formation on MPs. However, PES MPs reduced nitrate removal efficiency from 99.6 ± 0.3 % to 90.9 ± 7.9 % and decreased the relative abundance of denitrifying bacteria. Numerous taxa showed affinity to PE pellets, including some pathogens, e.g., Norcadia and Mycobacterium. Notably, an uncultured phylum Candidatus Saccharibacteria dominated in membrane biofilm and MPs, reaching up to 37 % relative abundance. This study is the first to explore how different types and shapes of MPs affect membrane bioreactor systems, particularly with respect to microbial community structure and biofilm formation. The findings offer new insights into the influence of MPs on wastewater treatment processes and highlight the significance of the uncultured phylumCandidatus Saccharibacteriain membrane fouling.

Aging affects the mechanical interaction between microplastics and lipid bilayers.

Plastic pellets, the pre-production form of many plastic products, undergo oxidation and photodegradation upon exposure to oxygen and sunlight, resulting in visible color changes. This study examines the impact of environmental aging on the mechanical interactions between pellet-derived microplastics and lipid bilayers, a critical component of biological membranes. Polyethylene pellets were collected from La Pineda beach near Tarragona, Spain, and categorized by chemical composition and yellowing index, an indicator of aging. The hydrophilicity of these pellets was assessed using contact angle measurements. Microplastics were produced by grinding and filtering these pellets and subsequently dispersed around a free-standing lipid bilayer within a 3D microfluidic chip to investigate their interactions. Our results reveal that aged microplastics exhibit a significantly increased adhesive interaction with lipid bilayers, leading to greater bilayer stretching. Theoretical modeling indicates a linear relationship between the adhesive interaction and the contact angle of the pellets, reflecting their hydrophilicity. These findings emphasize the increased mechanical impact of aged microplastics on biological membranes, which raises concerns about their potential toxicological effects on living organisms. This study highlights the importance of understanding the interactions between environmentally aged microplastics and biological systems to assess their risks, as these may differ significantly from pristine microplastics often studied under laboratory conditions.

Gradient columns to measure the density of microplastics

Density gradient columns are an established industrial method for measuring the density of plastics, but have rarely been applied to environmental plastics. In this study 14 density gradient columns were used to measure the density of 150 environmental plastics particles from an urban beach, plus 100 microplastics of known identity, representing what is believed to be the most extensive density dataset for environmental plastic debris available in scientific literature. In total, 92 % of investigated particles had their density measured, with the remainder falling outside of the range of the density columns: 800–1418 kg·m−3. Error values for individual plastic particles were conservatively estimated as ≤0.27 kg·m−3, equating to the density difference associated with a distance of 1 mm in the density gradient column. Moreover, error values for plastics of known identity, based on the standard deviation of five different particles of the same polymer type, were generally low, ≤± 1.78 kg·m−3 for 75 % of polymers. The most notable exception was crumb rubber from used tyres, with a density of 1204.84 ± 105.87 kg·m−3, reflecting a heterogenous material. The majority of environmental plastics were polyethylene pellets, with densities from 823.47 to 1143.47 kg·m−3, a much wider range than reported in literature for this polymer. The densest environmental pellet was biologically attached to a stone-like particle. Otherwise, there was no evidence that environmental processing, in the form of biofilm growth or weathering, was driving variability in density. Most pellets with extremely high or low density were coloured, indicating that additives or impurities introduced during manufacturing altered the density of the virgin resin. Overall, density gradient columns show great promise for improving our knowledge of microplastic density. They represent an accurate and efficient high-throughput method, which can measure the density of ∼40 microplastics simultaneously over relatively short time periods.

Development of jute-polyethylene nonwoven fabric for sustainable packaging application

ABSTRACT Environmental concerns promote demand for biodegradable packaging on a global scale. Jute fiber packaging could be a viable and sustainable alternative to pure synthetic materials. In this study, sustainable antimicrobial jute-polyethylene nonwoven fabric is developed by the heat press of jute web and polyethylene pellets. The performance of the developed samples was evaluated by analyzing their morphological, mechanical, thermal, moisture management, and antibacterial properties. SEM confirmed the homogeneous interfacial adhesion between jute-polyethylene. FTIR spectra proved the existence of jute, polyethylene, and peppermint oil in the developed samples. Mechanical property was investigated using a universal strength tester while tensile strength and elongation (%) were sufficient. The low thermal conductivities were observed in the samples. The moisture management tester confirmed the unavailability of the moisture in the inner surface from the outer surface. Furthermore, the samples exhibited significant antibacterial properties because of the application of peppermint essential oil.

The Use of Nonmetallic Fraction Particles with the Double Purpose of Increasing the Mechanical Properties of Low-Density Polyethylene Composite and Reducing the Pollution Associated with the Recycling of Metals from E-Waste

A restorative process, where the nonmetallic fraction from e-waste printed circuit boards is reused as a raw material for the conformation of a new polymer matrix composite with increased properties favoring its industrial applications, is proposed with a zero residues approach. Low density polyethylene pellets and nonmetallic fraction particles were mixed, and due to the generation of static electricity during the mixing process, the nonmetallic particles became attached to the polyethylene pellets; the blended material was fed into a screw extruder, producing filaments of the new composite. Mechanical properties increased as the particles content increased, presenting an ultimate tensile strength going from 20 for the raw low-density polyethylene to more than 60 MPa, and a yield strength that goes from 10 to 50 MPa on the composite with 6.0 wt.% particles. Also, the flammability of the composite improved, reducing its linear burning rate and increasing the time between detachment of two consecutive drops. Nonmetallic fraction particles were oriented in the extrusion direction and had a good adhesion with the polyethylene matrix. These composites can be employed for the production of prototypes using additive manufacture.

The tolerance of a keystone ecosystem engineer to extreme heat stress is hampered by microplastic leachates.

Plastic pollution and ongoing climatic changes exert considerable pressure on coastal ecosystems. Unravelling the combined effects of these two threats is essential to management and conservation actions to reduce the overall environmental risks. We assessed the capacity of a coastal ecosystem engineer, the blue mussel Mytilus edulis, to cope with various levels of aerial heat stress (20, 25, 30 and 35°C) after an exposure to substances leached from beached and virgin low-density polyethylene pellets. Our results revealed a significant interaction between temperature and plastic leachates on mussel survival rates. Specifically, microplastic leachates had no effect on mussel survival at 20, 25 and 30°C. In turn, mussel survival rates significantly decreased at 35°C, and this decrease was even more significant following an exposure to leachates from beached pellets; these pellets had a higher concentration of additives compared to the virgin ones, potentially causing a bioenergetic imbalance. Our results stress the importance of adopting integrated approaches combining the effects of multiple environmental threats on key marine species to understand and mitigate their potential synergistic effects on ecosystem dynamics and resilience in the face of the changing environment.

Screening of organic chemicals associated to virgin low-density polyethylene microplastic pellets exposed to the Mediterranean Sea environment by combining gas chromatography and liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry

In this work, organic chemicals associated with microplastics (MPs) exposed to a coastal anthropogenized environment for up to eight weeks have been screened for, in order to discern the (de)sorption dynamics of chemicals in the marine ecosystem. Low-density polyethylene (LDPE) pellets were studied since they represent primary MPs used by the plastic industry and a relevant input of MPs into the oceans. To maximize the coverage of chemicals that could be detected, both liquid and gas chromatography coupled to quadrupole-time-of-flight (GC-QTOF and LC-QTOF, respectively) were used. In the case of LC-QTOF, an electrospray ionization source was employed, and the compounds were investigated by combining suspect and non-target screening workflows. The GC-QTOF was equipped with an electron ionization source and compounds were screened in raw and derivatized (silylated) extracts by deconvolution and contrast to high- and low-resolution libraries. A total of 50 compounds of multifarious classes were tentatively identified. Among them, melamine and 2-ethylhexyl salicylate (EHS) were detected in the original MPs but were rapidly desorbed. Melamine was completely released into the marine environment, while EHS was partly released but a portion remained bound to the MPs. On the other hand, many other chemicals of both anthropogenic (e.g. phenanthrene or benzophenone) and natural origin (e.g. betaine and several fatty acids) accumulated onto MPs over time. Quantification of 12 unequivocally identified chemicals resulted into a total concentration of 810 μg/kg after MPs exposure for 8 weeks.

Ultrasonic measurement of fill volume of bulk solids in discharge vessels

In bulk solid handling, measuring the fill volume of vessels with powders is complex due to the uneven material surface. This research presents a method using an array of narrow-beam ultrasonic range finders in a powder discharge vessel. The vessel's cross-sectional area was divided into equal sub-sections, each measured by a range finder. The total fill volume was calculated by summing up the volumes of these sections. This system allows quick volume estimates at around 0.9 Hz under various flow states. In static conditions, tests on flat, concave, and convex powder surfaces showed a volume discrepancy of <5% of vessel capacity. For a vessel discharging polyethylene pellets, the estimated fill volume was under 0.6% uncertainty (confidence interval: 0.95). The findings underscore the utility of ultrasonic range finders for accurate volume determination in powder discharge vessels.

Polyethylene Biodegradation by an Artificial Bacterial Consortium: Rhodococcus as a Competitive Plastisphere Species.

Polyethylene (PE), a widely used recalcitrant synthetic polymer, is a major global pollutant. PE has very low biodegradability due to its rigid C-C backbone and high hydrophobicity. Although microorganisms have been suggested to possess PE-degrading enzymes, our understanding of the PE biodegradation process and its overall applicability is still lacking. In the present study, we used an artificial bacterial consortium for PE biodegradation to compensate for the enzyme availability and metabolic capabilities of individual bacterial strains. Consortium members were selected based on available literature and preliminary screening for PE-degrading enzymes, including laccases, lipases, esterases, and alkane hydroxylases. PE pellets were incubated with the consortium for 200 days. A next-generation sequencing ana-lysis of the consortium community of the culture broth and on the PE pellet identified Rhodococcus as the dominant bacteria. Among the Rhodococcus strains in the consortium, Rhodococcus erythropolis was predominant. Scanning electron microscopy (SEM) revealed multilayered biofilms with bacteria embedded on the PE surface. SEM micrographs of PE pellets after biofilm removal showed bacterial pitting and surface deterioration. Multicellular biofilm structures and surface biodeterioration were observed in an incubation of PE pellets with R. erythropolis alone. The present study demonstrated that PE may be biodegraded by an artificially constructed bacterial consortium, in which R. erythropolis has emerged as an important player. The results showing the robust colonization of hydrophobic PE by R. erythropolis and that it naturally possesses and extracellularly expresses several target enzymes suggest its potential as a host for further improved PE biodeterioration by genetic engineering technology using a well-studied host-vector system.

Impact of Hybrid Biomass Fillers on the Physico-Mechanical and Degradation Properties of Utility Polymers

The impact of cassava peel-rice husk hybrid fillers on the physico-mechanical and degradation properties of utility polymers were studied. Fine powders of cassava peel and rice husk of 75µm mesh size were homogeneously mixed at the ratio of 1:1 to form the hybrid filler. The virgin crystalline pellets of Low-Density Polyethylene and Polypropylene were filled with varying weight percentages (5, 10, 15, and 20) of the hybridized filler and the composites produced via the injection moulding technique at 288oC. The mechanical properties were studied according to ASTM standards. The surface morphological properties and thermal properties were studied with Scanning Electron Microscope and Differential Scanning Calorimeter respectively. The results of the mechanical properties showed improved tensile strength, compression strength and hardness as the filler load increases till optimum filler load was attained, while the percentage elongation at break decreased with increase in filler load. The shear modulus was found to be irregular. The creep behaviour with the PP composites was irregular but linear with LDPE composites. The solvent imbibition showed no increase in water, but less than 1% increase in toluene. The DSC result showed the hybrid filler had positive impact on LDPE composite but no effect on PP composite. The micrographs showed improved properties as the filler loading increases. Biodegradation test in soil revealed a reduction in the mass of the composites after a 6- month burial period indicating that the composite is degradable and eco- friendly. From the results, the cassava peel-rice husk hybrid filler can be employed in formulation of polymers where strength, water resistance and biodegradability is required such as plastic tiles. Thus, it is suggested that agro-wastes such as cassava peel and rice husk could be used as fillers in production of degradable plastics as they are accessible, cheap, easy to use and eco-friendly.

Biofilms retard the desorption of benzo(a)pyrene from polyethylene pellets in the marine environment

Microplastics are emerging as vectors for the transport hydrophobic organic compounds (HOCs) in aquatic environments, however, their impact is poorly understood due to the lack of field studies. In this study, the pristine and benzo(a)pyrene (B[a]P) adsorbed polyethylene (PE) pellets were placed at Haihe Estuary (Tianjin, China) for 80 days to investigate desorption behavior. Combining laboratory and in situ experiments, this study firstly verified that the intra-particle diffusion was the rate-limiting step for the desorption process of B[a]P from PE microplastics under different environmental conditions. By hindering the desorption and modifying MPs surface, biofilm might play a key role in desorption process, leading to the apparent hysteresis of the field desorption process at our time scale. Potential degradation of the polymer and B[a]P by biofilms, however, would support continuing desorption. The study explored the interaction of biofilm and MPs-contaminants mixture and its implications for the environmental fate of HOCs.

Polyethylene degradation and heavy metals leaching under realistic tropical marine climate

The study examines the influence of temperature and pH on the leaching of six heavy metals (HMs) species: aluminum (Al), zinc (Zn), chromium (Cr), copper (Cu), lead (Pb) and arsenic (As) from transparent polyethylene pellets into seawater. The idea is to understand the potential influence of intensifying global warming and ocean acidification towards microplastic toxicity in the ocean. HMs leaching was obvious by 24th hours, with most HMs concentration decreased in water by 120th and 240th hours except Al. Nevertheless, we report that temperature and pH do not influence the overall HMs leaching from PE pellets with statistical analysis showing no significance (p < 0.05) between temperature and pH toward HMs concentration. Instead, it is hypothesized that these two parameters may be crucial in promoting heavy metal adsorption onto PE pellets under tropical weathering. However, Field Emission Scanning Electron Microscope (FESEM) revealed that temperature and pH are influential in polymer aging and surficial breakdown where pellets exposed in warm, acidic waters showed the greatest extent of weathering. This study highlights that PE pellets exposed under tropical conditions may accelerate surficial degradation and possibly stimulate HMs adherence to the polymer as a pollution vector. Further consideration of metal behaviour in water and microbial activities is crucial to improve our understanding of microplastic toxicity under tropical weathering.

Polystyrene and low-density polyethylene pellets are less effective in arsenic adsorption than uncontaminated river sediment

The adsorption process of inorganic arsenic (As) plays an important role in its mobility, bioavailability, and toxicity in the river environment. In this work, the adsorption of dissolved arsenite (As(III)) and arsenate (As(V)) by microplastics (MPs) pellets (polystyrene (PS) and low-density polyethylene (LDPE)), river sediment, and their mixture were investigated to assess the adsorption affinities and mechanism. The adsorption kinetics showed slow and mild rising zones from the natural behavior of the chemical adsorption. The results indicated that both MP characteristics and water properties played a significant role in the adsorption behavior of inorganic As species. The As adsorption equilibrium was modeled well by both Langmuir and Freundlich isotherms and partly fitted with the Sips model suggesting that both mono-layer and multi-layer adsorption occurred during adsorption The spontaneous adsorption process for both As(III) and As(V) was evidenced by the adsorption thermodynamics. The maximum adsorption capacities of As(III) and As(V) reached 143.3 mg/kg and 109.8 mg/kg on PS in deionized water, which were higher than those on sediment-PS mixture (119.3 mg/kg, 99.2 mg/kg), which were all lower than on sediment alone (263.3 mg/kg, 398.7 mg/kg). The Fourier transform infrared spectroscopy analysis identified that As(III) and As(V) interaction with sediment surface functional groups was the main adsorption mechanism from surface complexation and coordination. Two functional groups of polystyrene (-NH2, -OH) were mainly involved in the adsorption of inorganic As species on PS, while -COO- and -OH functional groups contributed to the adsorption mechanism of inorganic As species on LDPE. The findings provide valuable insight on the adsorption behavior and mechanisms of As(III) and As(V) in river systems in the presence of MPs particles. Both PS and LDPE were shown to be less effective than river sediment in the adsorption of As species from water, which provides a different perspective in understanding the scale of MPs impact in pollutant transport in the aquatic environment.Graphical

Sustainable Filtering Systems to Reduce Microfiber Emissions from Textiles during Household Laundering.

During laundering, synthetic textiles (polyester, polyamide, etc.) can release small fiber debris with a length of <5 mm. These are a type of microplastics (MPs), usually referred to as microfibers (MFs), which are considered high-concern pollutants due to their continuous and cumulative entrance into the environment. Currently, as far as we know, there are no feasible alternatives to remove them. In this work, four new and sustainable filtering systems are proposed to retain the MFs emitted from domestic washing machines. The filters contain a replaceable cartridge partially filled with recycled low-density polyethylene pellets. The four designed filtering systems of different sizes were tested in a household washing machine determining the retention efficiency of the MFs after several washing cycles. It was found that all four assessed filter arrangements have a good performance for retaining MFs from the washers' effluents. Filter F1 (diameter of 4 cm and a height of 30 cm) started retaining more than 50% of the MFs, at the 10th washing cycle, the retention climbed to 66%, while in the 20th washing cycle, its retention was greater than 80%. MFs retention was higher for filter F2 (diameter of 6.3 cm and a height of 41 cm), achieving a performance greater than 90% in the 20th washing cycle. Filter F3 was arranged by turning the F1 model flow upside down and the retention efficiency is higher compared with filter F1 values, reaching a retention efficiency of almost 100% in the 15th washing cycle. Finally, filter F4 arrangement was developed using the existing washing machine filter, obtaining better performance than the F1 and F2 filters, reaching efficiencies higher than 90% at the 20th washing cycle. In summary, depending on the arrangement, the microfiber retention efficiency was estimated between 52% and 86% in the 1st washing cycle and up to 83% to 99% in the 20th. Additionally, all arrangements demonstrated that the cartridges may last for more than 30 washing cycles before needing to be replaced.

Partitioning of heavy metals in sediments and microplastics from stormwater runoff

Microplastics could act as vehicles for transporting heavy metals from urban environments to water resources via stormwater runoff. Although the transport of heavy metals by sediments has been widely studied, there is a lack of mechanistic understanding of their competition with microplastics (MPs) for heavy metal uptake. Therefore, this study was conducted to examine the partitioning of heavy metals in microplastics and sediments from stormwater runoff. For this purpose, new low density polyethylene (LDPE) pellets were selected as representative MPs, and accelerated UV-B irradiation experiments were conducted for eight weeks to generate photodegraded MPs. The competition of Cu, Zn, and Pb species for the occupation of available surface sites on sediments and new and photodegraded LDPE MPs was examined through 48 h kinetics experiments. Additionally, leaching experiments were conducted to identify the extent of organics released into the contact water by new and photodegraded MPs. Moreover, 24 h metal exposure experiments were conducted to identify the role of initial metal concentrations on their accumulation onto the MPs and sediments. The photodegradation process altered the LDPE MPs’ surface chemistry by creating the oxidized carbon functional groups [>CO, >C–O–C<], and it also enhanced their dissolved organic carbon (DOC) leaching into the contact water. The results showed significantly greater levels of Cu, Zn, and Pb accumulations on photodegraded MPs compared to the new MPs in either absence or presence of sediments. Heavy metal uptake by sediments when photodegraded MPs were present was significantly reduced. This might be due to the organic matter leached by photodegraded MPs into the contact water.

Hydrophobic interface-assisted synthesis of K2CO3/Al2O3 adsorbent pellets for CO2 capture

Suitable molding and granulation methods are crucial, and an outstanding sphere structure can play a better role in the future scale-up industrial application of K2CO3-based CO2 adsorbent. Water will form a large contact angle on the surface of hydrophobic solution and become sphericity water. In this work, hydrophobic powders (polyethylene (PE), TiO2, and SiO2) were selected in the hydrophobic interface-assisted method. The CO2 adsorption capacity of TiO2 pellets is 0.50 mmol/g, while that of PE pellets is 0.24 mmol/g, and finally that of SiO2 pellets is 0.36 mmol/g. Then, experiment proved that high temperature (>500 ℃) makes the hydrophobic powders (i.e, TiO2 and SiO2) lose hydrophobicity which make water molecules diffuse faster, while PE is completely decomposed at high temperature. Compared with the pellets calcined at 300 ℃, the TiO2 pellets calcinated at 500 ℃ have an adsorption capacity of 0.70 mmol/g, which is over 1.72 times that of the original pellets, and the other two hydrophobic surfaces are also improved to some extent. Additionally, the appropriate ratio of low-temperature pore forming agent (i.e., urea) can help improve the adsorption performance, while excessive addition will lead to poor CO2 adsorption performance. In addition, the K2CO3/Al2O3 pellets also exhibited desirable mechanical properties, with an optimal compressive strength of 41 Mpa. Therefore, the loss of hydrophobicity leads to more active diffusion of water molecules, which may provide a new idea for the future development of CO2 adsorbents and other areas.

Spatiotemporal characterisation of microplastics in the coastal regions of Singapore

In the 21st century, plastic production continues to increase at an unprecedented rate, leading to the global issue of plastic pollution. In marine environments, a significant fraction of plastic litter are microplastics, which have a wide range of effects in marine ecosystems. Here, we examine the spatiotemporal distribution of microplastics along the Johor and Singapore Straits, at surface and at depth. Generally, more microplastics were recorded from the surface waters across both Straits. Fragments were the dominant microplastic type (70%), followed by film (25%) and fiber (5%). A total of seven colours of microplastics were identified, with clear microplastics as the most abundant (64.9%), followed by black (25.1%) and blue (5.5%). Microplastics under 500 μm in size accounted for 98.9%, followed by particles 500–1000 μm (1%) and 1–5 mm (0.1%). During the monsoon season, the abundance of microplastics across various sites were observed to be > 1.1 times when compared to the inter-monsoon period. Rainfall was a closely related to the increased microplastic abundance across various sites in the Singapore Strait. This suggests that weather variations during climate change can play critical roles in modulating microplastic availability. Beach sediments facing the Singapore Strait recorded an abundance of 13.1 particles/kg, with polypropylene fragments, polyethylene pellets and thermoplastic polyester foam identified via Fourier transform infrared spectroscopy. Hence, it is crucial to profile the spatiotemporal variation of microplastic abundance in both the surface and in the water column to gain a better understanding of the threat caused by microplastic pollution in the coastal regions of Singapore.

Produção de fertilizante foliar de alta solubilidade em secador de leito de jorro

ABSTRACT The drying process can be useful to change the particulate structure, improving the powder solubility. In this study, we investigated the drying of foliar fertilizer in a spouted bed using polyethylene pellets as inert particles to improve its solubility. A 23 factorial experimental design was used to evaluate how the powder yield and moisture were affected by the feeding time, intermittency time, and feed atomizer position. The spouting instability caused powder retention on the bed wall, which decreased the powder recovery efficiency by 2.3-26.6%. Although the powder recovery efficiency was low, the solubility time was reduced by 5.9 times, probably due to particle agglomeration, which increased the amorphous phase of the fertilizer. Design alterations, suggested by the CFD data, can increase spouted bed stability and facilitate centralized spouting. Based on this, the spouted bed drying technique was applied, and it effectively increased the solubility of commercial fertilizer, thus, incorporating more desirable characteristics for field applications.

Effects of mechanical recycling on optical properties and microstructure of recycled high‐density polyethylene pellets and bottles

AbstractThe demand for recycling high‐density polyethylene (HDPE) utilizing mechanical recycling technologies is currently felt strongly by both society and industry. However, thermal oxidation of the polymer during the recycling process may lead to irreversible changes in the material properties of recycled high‐density polyethylene (rHDPE). The effects of mechanical recycling on the optical characteristics and microstructure of rHDPE pellets and bottles were investigated in this study. The results revealed that the apparent color of the rHDPE became more yellow and gray compared to the virgin HDPE (vHDPE), and showed a signal at 670–680 nm in the solar reflectance spectrum. The thermal oxidation of rHDPE considerably raised the absorption intensities of carbonyl, ester, and hydroxyl groups in attenuated total reflection Fourier transform infrared spectrum. In addition, the presence of carbonyl and hydroxyl unsaturated chemicals might make it challenging to recognize the distinctive peaks of vHDPE in the ultraviolet–visible diffuse reflectance (UV–Vis‐DIR) spectra at wavelengths less than 400 nm. Thermal oxidation of rHDPE was also confirmed in the COH, CO, and OCO valence structures of C1s and O1s. A characteristic valence band (VB) profile at 25 eV can be used as the recognizable information for the oxidation of rHDPE. The microstructure of the surface of rHDPE pellets exhibited rough and uneven morphological defects. The higher recycled content made rHDPE bottles' surface morphology rougher and their cross‐section microstructure thinner and more porous than vHDPE bottles.

Selection of microorganisms capable of polyethylene (PE) and polypropylene (PP) degradation

Microorganisms degrade microplastics, but their potential is still not fully exploited, e.g., due to inadequate selection of microorganisms. We developed an effective selection method of microorganisms capable of polyethylene (PE) and polypropylene (PP) degradation and assessed the scale of polymer degradation by microbial populations.We isolated seven bacterial strains (three Priestia megaterium strains, Klebsiella pneumoniae, Pseudomonas fluorescens, Enterobacter ludwigii, Chryseobacterium sp.) and seven fungal strains (four Fusarium spp., two Lecanicillium spp. and Trichoderma sp.) with PE degradation potential, as well as seven bacterial strains (five Serratia marcescens and two Enterobacter spp.) and six fungal strains (four Aspergillus spp., Fusarium oxysporum and Penicillium granulatum) with PP degradation ability. Scanning electron microscopy (SEM) analysis confirmed the presence of a biofilm and revealed surface changes in both PE and PP pellets, but the greatest changes (microcracks and corrugations) were observed for PP incubated with bacteria. Fourier transform infrared (FTIR) spectroscopy confirmed the structural changes on the studied polymer surfaces.In conclusion, the isolation of plastic-degrading bacteria and fungi from waste landfills represents an effective strategy for the collection of microorganisms with high potential for PE and PP degradation. The bacteria and fungi revealed better potential for PP degradation and PE degradation, respectively.