The mechanical properties of polyelectrolyte multilayer films built up with poly-l-lysine (PLL) and hyaluronan (HA) can be tuned using a chemical cross-linker. The present study is aimed at showing that the viscoelasticity can also be changed by exposing a (PLL/HA)m film alternatively to a solution of poly(sodium 4-styrene sulfonate) (PSS) and a solution of poly(allylamine hydrochloride) (PAH), thus forming (PLL/HA)m-(PSS/PAH)n-PSS films. Force curves have been recorded with an atomic force microscope using a micrometric spherical probe and different approach velocities ranging over 2−3 orders of magnitude. These curves are analyzed in the framework of the Hertzian mechanics corrected for the finite thickness of the film deposited on a hard (glass) substrate, with the indentation limited to 50 nm to preserve material linearity. The force curves cannot be reproduced satisfactorily when the probed films are assumed to be elastic bodies. Better agreement is achieved when the films are depicted as Maxwell bod...