Molecular Ions Research Articles

Plasma and gas neutralisation of high energy H- and D- in an Argon plasma neutraliser

A beam driven plasma neutraliser (BDPN) has been proposed [1] as a relatively simple way to increase the efficiency of neutral beam injectors based on the acceleration and neutralisation of H- or D-. Initial calculations showed that sufficient levels of ionisation could be achieved with H2 or D2 as the initial gas target in the neutraliser if a plasma confinement time of the plasma in the neutraliser is >0.5 ms could be achieved. However, later calculations [2,3] show that the afore-mentioned results were very optimistic as the calculations did not take into account the recombination of molecular ions with electrons. Hence, it has been suggested [4] to use an argon (Ar) plasma as the molecular ion content in such a plasma will be negligible. The use of an Ar plasma neutraliser has been suggested previously [5–7], but few details have been given of the obtainable neutralisation efficiency, the necessary line density for that neutralisation efficiency, or of the calculations and cross sections used to deduce those quantities. This paper briefly discusses why Ar could be a good choice for a plasma neutraliser, then the reactions occurring between the H-/D- beam and the particles in the Ar plasma, and the available cross section data for those reactions. Subsequently, the differential equations for the change of the species in the beam as it traverses an Ar plasma are deduced and solved, and the solutions used to calculate the achievable neutralisation efficiency as a function of the line density in the neutraliser etc. Results are given of the neutralisation efficiency as a function of the degree of ionisation in the neutraliser, the required line density, and the species changes in the beam for accelerated H- beams with 100, 500 and 870 keV energies.

Electric field-induced electronic structure and intersubband transitions of a hydrogen molecular ion in a gaussian-type quantum ring

In this work, the influence of an external electric field on the electronic structure and intersubband transitions of a singly ionized double-donor system in a GaAs quantum ring defined by Gaussian-type potentials is investigated theoretically. Within the framework of the effective mass approach, the two-dimensional diagonalization method is used for the solution of the Schrödinger equation to obtain the eigen energies and corresponding wave functions. Numerical results reveal that the electronic energy spectrum and the linear optical absorption coefficient of the ring are remarkably affected by the strength of the lateral electric field, internuclear distance and parameters defining the confinement potential. Also, it has been shown that beyond the anti-crossing point, the wave functions exchange their symmetries without mixing, which is a characteristic feature of energy-level anti-crossing.

UPLC-LTQ-Orbitrap Study on Rat Urinary Metabolites of 5-Methoxy-Alpha-Methyltryptamine.

5-Methoxy-α-Methyltryptamine (5-MeO-AMT) is a new psychoactive substance which is abused due to its hallucinogenic and euphoric effects. This study aimed to study the metabolic characteristics of 5-MeO-AMT. Five rats were given intraperitoneal injection at a dose of 50 mg/kg of 5-MeO-AMT, and their urine was subsequently collected at different times within 7 days. Ultra-high performance liquid chromatography-- tandem high-resolution mass spectrometry (UPLC-LTQ-Orbitrap) was used to detect the precise molecular weight and fragment ions of 5-MeO-AMT and its possible metabolites in the urine sample extracted with benzene-ethyl acetate. Three metabolites, including OH-5-MeO-AMT, α-Me-5-HT, and N-Acetyl-5-MeO-AMT were identified in rats' urine. The major metabolic pathways involved O-demethylation, hydroxylation of indole ring, and Acetylation on aliphatic amines. The results of this study are an important reference for the identification and screening of toxicants of 5-MeO-AMT.

Discovery of Unprecedented Human Stercobilin Conjugates.

Two unique metabolites (M18 and M19) were detected in feces of human volunteers dosed orally with [14C]inavolisib with a molecular ion of parent plus 304 Da. They were generated in vitro by incubation with fecal homogenates and we have evidence that they are formed chemically and possibly enzymatically. Structural elucidation by high resolution mass spectrometry and nuclear magnetic resonance spectroscopy showed that the imidazole ring of inavolisib was covalently bound to partial structures derived from stercobilin, an end-product of heme catabolism produced by the gut microbiome. The structural difference between the two metabolites was the position of methyl and ethyl groups on the pyrrolidin-2-one moieties. We propose a mechanism of M18 and M19 generation from inavolisib and stercobilin whereby nucleophilic attack from the imidazole ring of inavolisib occurs to the bridging carbon of a stercobilin molecule. The proposed mechanism was supported by computational calculations of molecular orbitals and transition geometry. SIGNIFICANCE STATEMENT: We report the characterization of two previously undescribed conjugates of the phosphoinositide 3-kinase inhibitor inavolisib, generated by reaction with stercobilin, an end-product of heme catabolism produced by the gut microbiome. These conjugates were confirmed by generating them using in vitro fecal homogenate incubation via nonenzymatic and possibly enzymatic reactions. Given the unique nature of the conjugate, it is plausible that it may have been overlooked with other small molecule drugs in prior studies.

Magnetic ligand fishing protocol combined with HPLC-FT-ICR-MS for screening potential α-Glucosidase inhibitors from UCG and in silico analysis

Uremic Clearance Granule (UCG) is a traditional Chinese medicines (TCMs) that has been recognized as potentially effective treatment for diabetic nephropathy (DN). However, the chemical composition and the hypoglycemic ingredients of UCG remain unclear. In this experiment, a magnetic ligand fishing protocol combined with HPLC coupled Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FT-ICR-MS) technology was developed to screen and identify α-Glucosidase inhibitors from UCG. First, constituents of UCG was identified by HPLC-FT-ICR-MS. Next, α-Glucosidase coated Fe3O4@SiO2@NH2 (Fe3O4@SiO2@NH2@α-Glucosidase) nano composites was synthesized. The essential parameters (concentration of Glutaraldehyde (GA), ratio of Fe3O4@SiO2@NH2 to enzyme, crosslinking time and immobilization time) were optimized to obtain maximum enzyme activity and the performance of immobilized enzyme was compared with that of free enzyme. The α-Glucosidase inhibitory activities were then evaluated using autodock and the 4-Nitrophenyl-α-D-glucopyranoside (pNPG) method. As a result, 142 compounds were identified were identified by comparing the retention time, molecular ions and fragmentation behaviors with the reference compounds or the in-house database. 20 possible α-Glucosidase activity inhibitory components were identified using HPLC-FT-ICR-MS. Molecular docking and inhibition studies showed that 4 compuunds including Limonexic acid, Sanggenol A, Glabrone, Matrine were more likely to stronger α-Glucosidase inhibitory activities than acarbose. In conclusion, the proposed approach, which combined highly specific screening with HPLC-FT-ICR-MS, provided a powerful platform for discovering bioactive components from multi-component and multi-target traditional Chinese medicines (TCMs).

Rapid analysis of untreated food samples by gel loading tip spray ionization mass spectrometry.

Rapid, efficient, versatile, easy-to-use, and non-expensive analytical approaches are globally demanded for food analysis. Many ambient ionization approaches based on electrospray ionization (ESI) have been developed recently for the rapid molecular characterization of food products. However, those approaches mainly suffer from insufficient signal duration for comprehensive chemical characterization by tandem MS analysis. Here, a commercially available disposable gel loading tip is used as a low-cost emitter for the direct ionization of untreated food samples. The most important advantages of our approach include high stability, and durability of the signal (> 10min), low cost (ca. 0.1 USD per run), low sample and solvent consumption, prevention of tip clogging and discharge, operational simplicity, and potential for automation. Quantitative analysis of sulfapyridine, HMF (hydroxymethylfurfural), and chloramphenicol in real sample shows the limit-of-detection 0.1μgmL-1, 0.005μgmL-1, 0.01μgmL-1; the linearity range 0.1-5μgmL-1, 0.005-0.25μgmL-1, 0.01-1μgmL-1; and the linear fits R2 ≥ 0.980, 0.991, 0.986. Moreover, we show that tip-ESI can also afford sequential molecular ionization of untreated viscous samples, which is difficult to achieve by conventional ESI. We conclude that tip-ESI-MS is a versatile analytical approach for the rapid chemical analysis of untreated food samples.

Femtosecond Laser Ionization Mass Spectrometry for Online Analysis of Human Exhaled Breath.

A variety of organic compounds in human exhaled breath were measured online by mass spectrometry using the fifth (206 nm) and fourth (257 nm) harmonic emissions of a femtosecond ytterbium (Yb) laser as the ionization source. Molecular ions were enhanced significantly by means of resonance-enhanced, two-color, two-photon ionization, which was useful for discrimination of analytes against the background. The limit of detection was 0.15 ppm for acetone in air. The concentration of acetone in exhaled breath was determined for three subjects to average 0.31 ppm, which lies within the range of normal healthy subjects and is appreciably lower than the range for patients with diabetes mellitus. Many other constituents, which could be assigned to acetaldehyde, ethanol, isoprene, phenol, octane, ethyl butanoate, indole, octanol, etc., were observed in the exhaled air. Therefore, the present approach shows potential for use in the online analysis of diabetes mellitus and also for the diagnosis of various diseases, such as COVID-19 and cancers.

Isolation of Arborescin from Artemisia absinthium L. and Study of Its Antioxidant and Antimicrobial Potential by Use of In Vitro and In Silico Approaches

This study focused on developing an innovative, straightforward, and economical method utilizing a mixture of readily available solvents to extract arborescin (C2OH2OO8) crystals from Artemisia absinthium L. (A. absinthium). The structural elucidation and characterization were conducted using a suite of techniques including IR spectroscopy, CNHSO elemental analysis, scanning electron microscopy and energy dispersive X-ray spectroscopy (SEM-EDS), and mass spectroscopy (MS). Density functional theory (DFT) calculations were employed to determine the molecular properties. Antioxidant activity was measured using the DPPH radical scavenging assay and the β-carotene bleaching test. Antimicrobial efficacy was assessed against four bacterial strains and three fungal strains. The molecular docking approach was employed to predict the probable binding patterns and affinities of arborescin with specific target biomolecules. Employing an array of analytical techniques, examination of the isolated crystal from A. absinthium. led to its comprehensive structural elucidation. IR spectroscopy revealed the presence of distinctive functional groups, including a carbonyl group within the γ-lactone and an epoxy group. CNHSO elemental analysis verified that the crystal contained only carbon, hydrogen, and oxygen, a finding corroborated by SEM-EDS analysis, consistent with the molecular structure of arborescin. Additionally, mass spectrometry confirmed the identity of the compound as arborescin, with a molecular ion with a mass m/z = 248. Quantum-Chemical Descriptors revealed that arborescin is resistant to elementary decomposition under standard conditions. Although arborescin demonstrates a relatively low antioxidant capacity, with an IC50 of 5.04 ± 0.12 mg/mL in the DPPH assay, its antioxidant activity in the β-carotene bleaching test was found to be 3.64%. Remarkably, arborescin effectively inhibits the growth of Staphylococcus aureus and Listeria innocua at low concentrations (MIC = 166 µg/mL). Additionally, it exhibits significant antifungal activity against Candida glabrata, with a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of 83 µg/mL and 166 µg/mL, respectively. In this study, arborescin exhibited a robust docking score of −8.1 kcal/mol, indicating a higher affinity compared to ciprofloxacin. This suggests that arborescin has significant potential as a potent antibacterial agent.

A new 915 MHz coaxial-line-based microwave plasma source

Microwave plasma is known for its versatility in providing tailored operating conditions (pressure, working gas composition and residence time of reagents) for specific applications. Microwave plasma sources (MPSs) are vital in modern applications, demanding continuous improvement. This work introduces a coaxial-line-based nozzleless MPS that operates at atmospheric pressure at an unique frequency of 915 MHz. The measured electrodynamic characteristics in nitrogen of the MPS highlighted the need for improved energy efficiency of the device. The main novelty of this work lies in improving an energy efficiency of the presented MPS, which led to an advanced new version of the device. To achieve this, a dual strategy is employed. Firstly, numerical simulations are used to design a construction modifications to the MPS, which should increase the efficiency of transferring microwave energy from the microwave source to the generated plasma. In this step, a standard model for homogeneous plasma and a two-port equivalent method were used. Then, the theoretical results were experimentally validated by manufacturing a new energy improved version of the MPS. In the new MPS the achieved reflected microwave power (losses) was less than 3% of incident microwave power in the tested range of nitrogen flow rate (50–100 Nl/min). Compared to the MPS before improvement, this means a two-fold decreasing the reflected microwave power. To test the new MPS, the electrodynamic characteristics of the new device version and properties of the microwave plasma generated in nitrogen, using optical emission spectroscopy (OES), were investigated. The OES was used to determine the vibrational Tvib and rotational Trot temperatures of nitrogen molecules and molecular ions. In this work, the estimated Tvib and Trot temperatures for nitrogen molecules ranged from 4000 to 5300 K, depending on discharge conditions, while for nitrogen molecular ions, the temperatures changed between 4700 and 6100 K, respectively. Both the Tvib and Trot temperatures decrease linearly along the plasma flame.

Isolation and characterization of synthesis intermediates and side products in hexahydrocannabiphorol

AbstractAfter the Swiss ban of hexahydrocannabinol (HHC) in March 2023, other semisynthetic dibenzopyran cannabinoids emerged on the Swiss gray market. Hexahydrocannabiphorol (HHCP) was the most prominent of them due to its potent cannabimimetic effects, as anecdotal reports from recreational users suggest. In October 2023, a class wide ban of dibenzopyran cannabinoids was introduced in Switzerland to prevent new similar substances from entering the drug market. Various vendors in online shops claim that HHCP is made from CBD, even though they possess different alkyl chain lengths. An HHCP sample was analyzed by gas chromatography coupled to mass spectrometry (GC‐MS), showing that a mixture of molecules with the same or a similar molecular mass as HHCP was present. Six different substances could be isolated from this sample using column chromatography. Four phenols ((9R)‐HHCP, iso‐HHCP, cis‐HHCP, and abn‐HHCP) and two ketones (possible intermediates to (9R)‐HHCP and abn‐HHCP) were identified by various nuclear magnetic resonance spectroscopy (NMR) techniques. (9S)‐HHCP was obtained in an impure fraction. In addition, a fraction was obtained that showed characteristic molecular and fragment ions consistent with bisalkylated products from the synthesis of similar compounds. The presence of abnormal cannabinoids (abn‐HHCP) and bisalkylated cannabinoids is a confirmation that this sample was produced purely synthetically as initially suspected, as these compounds have not been reported in Cannabis. Chiral derivatization of the phenols with Mosher acid chlorides showed that only iso‐HHCP was present as a scalemic mixture, indicating a good stereocontrol of this synthetic procedure.

Operando-reconstructed polyatomic ion layers boost the activity and stability of industrial current–density water splitting

Metal–organic frameworks have garnered attention as highly efficient pre-electrocatalysts for the oxygen evolution reaction (OER). Current structure–activity relationships primarily rely on the assumption that the complete dissolution of organic ligands occurs during electrocatalysis. Herein, modeling based on NiFe Prussian blue analogs (NiFe-PBAs) show that cyanide ligands leach from the matrix and subsequently oxidize to corresponding inorganic ions (ammonium and carbonate) that re-adsorb onto the surface of NiFe OOH during the OER process. Interestingly, the surface-adsorbed inorganic ions induce the OER reaction of NiFe OOH to switch from the adsorbate evolution to the lattice-oxygen–mediated mechanism, thus contributing to the high activity. In addition, this reconstructed inorganic ion layer acting as a versatile protective layer can prevent the dissolution of metal sites to maintain contact between catalytic sites and reactive ions, thus breaking the activity–stability trade-off. Consequently, our constructed NiFe-PBAs exhibit excellent durability for 1250 h with an ultralow overpotential of 253 mV at 100 mA cm−2. The scale-up NiFe-PBAs operated with a low energy consumption of ∼4.18 kWh m−3 H2 in industrial water electrolysis equipment. The economic analysis of the entire life cycle demonstrates that this green hydrogen production is priced at US$2.59/kgH2, meeting global targets (<US$2.5/kgH2).

THz vs NIR laser-assisted atom probe tomography of LaB6 samples

Terahertz (THz) radiation with low-energy photons (meV) is used in a wide range of applications, such as microscopy, sensing, and spectroscopy. However, recently, high amplitude THz pulses of MV/cm have been generated and used for electron emission and ion evaporation from field emitters, opening up the possibility of using high amplitude THz pulses for material imaging by THz-assisted atom probe tomography (APT). In this work, we compare the APT analyses of lanthanum hexaboride (LaB6) samples using a femtosecond near-infrared laser with those obtained using high-amplitude single-cycle THz pulses. The atomic-scale characterization of stoichiometric LaB6 is challenging in laser-assisted APT due to the detection losses of boron ions. Here, we show that the THz radiation reduces the emission of molecular ions and multiple detection events, and it increases the charge state of the emitted ions. All these effects result in an improvement in boron detection. Furthermore, the emission dynamics of boron and lanthanum ions differ in their evaporation times when using THz radiation. This work emphasizes the ability of high-amplitude, single-cycle THz pulses to well control material analysis in APT, leading to better results on chemical composition. It also paves the way for the use of this radiation for material manipulation.

GC/MS analyses, FTIR and quantum mechanics calculations of poly Vinyl Alcohol in neutral and irradiated state

The products obtained from a poly vinyl alcohol (PVA) at non-irradiated and irradiated state were identified. The analyses were carried out using Head Space (HS) – Gas Chromatography – Mass Spectrometry (HS-GC/MS) instruments. On the other hand, FTIR spectroscopy has been done to explain the functional groups present. Also, the quantum chemical calculations using DFT (Density Function Theory) method were used to confirm the fragmentation process of the GC/MS chromatogram and the mass spectra of non-irradiated and irradiated PVA products. The HS-GC/MS analysis exhibited that fourth according to namely: 2-Butene, 2-methyl- (MW = 70), Cyclohexene (MW = 82), Benzocyclopropene (MW = 90), and Benzene, 1,2,4-trimethyl (MW = 120) have been detected and identified according to its GC/MS chromatogram outcomes. The mass spectrum of each component has been recorded and confirms the molecular ion peak. The obtained results show that the peaks begin to develop as the irradiation dose increases, and that this proportion rises as the gamma dose increases. Furthermore, every component of the fragment appears in the GC/MS chromatogram at a higher relative intensity of Selected ion motoring (SIM) as gamma dosages increase for each molecule. An induced change in the band intensities has been observed in FTIR spectra, which indicates cleavage in the CH bond after irradiation. In both cases, it was found a significant difference between the fragmentation products of the PVA at non-irradiated and irradiated state. Density functional theory (DFT) provides theoretical confirmation of this for both irradiated and non-irradiated PVA states.

Scanning Probe Microscopy Characterization of Biomolecules enabled by Mass-Selective, Soft-landing Electrospray Ion Beam Deposition.

Scanning probe microscopy (SPM), in particular at low temperature (LT) under ultra-high vacuum (UHV) conditions, offers the possibility of real-space imaging with resolution reaching the atomic level. However, its potential for the analysis of complex biological molecules has been hampered by requirements imposed by sample preparation. Transferring molecules onto surfaces in UHV is typically accomplished by thermal sublimation in vacuum. This approach however is limited by the thermal stability of the molecules, i. e. not possible for biological molecules with low vapour pressure. Bypassing this limitation, electrospray ionisation offers an alternative method to transfer molecules from solution to the gas-phase as intact molecular ions. In soft-landing electrospray ion beam deposition (ESIBD), these molecular ions are subsequently mass-selected and gently landed on surfaces which permits large and thermally fragile molecules to be analyzed by LT-UHV SPM. In this concept, we discuss how ESIBD+SPM prepares samples of complex biological molecules at a surface, offering controls of the molecular structural integrity, three-dimensional shape, and purity. These achievements unlock the analytical potential of SPM which is showcased by imaging proteins, peptides, DNA, glycans, and conjugates of these molecules, revealing details of their connectivity, conformation, and interaction that could not be accessed by any other technique.

Unravelling structural features of small molecules for photochemical transformation of environmental contaminants

Small molecules, including natural metabolites, organic matter decomposition products, and engineered oxidation byproducts, are widespread in aquatic environment. However, the limited understanding of the photochemical interactions of these small molecules with water pollutants hampers the development of effective environmental protection strategies. This study explores the structural features governing the photochemical transformation of toxic oxyanions by α- and β-dicarbonyl compounds. By integrating experimental observations with quantum chemical calculations, a robust correlation network was constructed. The correlation network reveals that the reactivity of small organic molecules with oxyanions could be quantitively predicted by their intrinsic properties, such as electronic transition energy, bond dissociation energy, molecular softness, molecular orbital gap, atomic charge, and molecular surface local ionization energy. This network maps the relationship between the molecular architecture of chemicals and their photochemical behaviors. This perspective offers fresh insights into the photochemical behaviors of small molecules in diverse environmental and chemical contexts and are helpful for developing advanced water treatment strategies toward a sustainable future.

Direct Measurement of Charge Transfer Probability during Photodissociation of Few-keV OD+ Beam.

We have measured the photodissociation of few-keV OD+ molecular ions into either D+ + O or O+ + D final products. The three-dimensional momentum imaging measurements of the light and massive fragments in coincidence were enabled by using an upgraded two-detector setup. In this work, we show that absorption of a single 790 or 395 nm photon excites the OD+ from its electronic ground state to the B state, which dissociates to the O+(4S) + D dissociation limit. To reach the other nearly degenerate dissociation limit, D+ + O(3P), a unimolecular charge transfer, B to X , transition is required following the same photoexcitation. The measured branching ratio of these dissociation channels is a direct measure of the charge transfer transition probability. This measured probability as a function of energy above the dissociation limit agrees well with our calculations.

Brief communication: New method for measuring nitrogen isotopes in tooth dentine at high temporal resolution.

Nitrogen isotopes (δ15N) are widely used to study human nursing and weaning ages. Conventional methods involve sampling 1-mm thick sections of tooth dentine-producing an averaging effect that integrates months of formation. We introduce a novel protocol for measuring δ15N by multicollector secondary ion mass spectrometry (SIMS). We sampled dentine δ15N on a weekly to monthly basis along the developmental axis in two first molars of healthy children from Australia and New Zealand (n = 217 measurements). Nitrogen isotope ratios were determined from measurements of CN- secondary molecular ions in ~35 μm spots. By relating spot position to enamel formation, we identified prenatal dentine, as well as sampling ages over more than 3 years. We also created calcium-normalized barium and strontium maps with laser ablation-inductively coupled plasma-mass spectrometry. We found rapid postnatal δ15N increases of ~2‰-3‰, during which time the children were exclusively breastfed, followed by declines as the breastfeeding frequency decreased. After weaning, δ15N values remained stable for several months, coinciding with diets that did not include meat or cow's milk; values then varied by ~2‰ starting in the third year of life. Barium did not show an immediate postnatal increase, rising after a few months until ~1-1.5 years of age, and falling until or shortly after the cessation of suckling. Initial strontium trends varied but both individuals peaked months after weaning. Developmentally informed SIMS measurements of δ15N minimize time averaging and can be precisely related to an individual's early dietary history.

Fire fighters and mass spectrometry: from the world of combustion to the molecular ion

Abstract The background and current studies in the area of fire investigation at the Turin Fire Department’s Forensic Laboratory. It is uncommon to find an investigation facility, and even less often within fire brigade headquarters, that uses GC-MS and GC-TOF extensively to look for accelerants. During 16 years of intense effort, the Turin Fire Department in Italy accomplished various research projects on the identification of flame accelerants and more in fire residues. This collaborative effort between academics and firefighters is unique. It is based on the perspectives of individuals who often fight fires before going on to investigation. The article will outline the study that was done, explain how advances in instrumentation have produced ever-better outcomes and the next steps in the foreseeable future.

Analytical characterization of anthocyanins using trapped ion mobility spectrometry-quadrupole time-of-flight tandem mass spectrometry

Anthocyanin profiles of juices from blueberry (Vaccinium myrtillus L.) and different grape varieties (Vitis labrusca L. cv. Concord, Vitis vinifera L. cvs. Accent, Dunkelfelder, Dakapo, and GM 674–1) were characterized by ultra-high performance liquid chromatography (UHPLC) coupled to trapped ion mobility spectrometry-quadrupole time-of-flight tandem mass spectrometry (TIMS-QTOF-MS/MS). Ion mobility and collision cross section (CCS) values of over 50 structurally related anthocyanins based on delphinidin, cyanidin, petunidin, peonidin, and malvidin were determined. Relations between molecular mass, mobility values, and specific structural features were revealed. The mass-to-charge (m/z) ratio of the molecular ions (M+) was found to be the major factor influencing anthocyanin ion mobilities, but structural characteristics also contributed to their variability. We were able to differentiate positional and geometrical isomers and certain epimers by their respective mobility values. For instance, whereas 3-O-hexosides (i.e., 3-O-glucosides and 3-O-galactosides) were separated by TIMS, epimers of 3-O-pentosides assessed could not be distinguished.

Ionospheric irregularities at Jupiter observed by JWST

Jupiter’s upper atmosphere is composed of a neutral thermosphere and charged ionosphere. In the latter, the dominant molecular ion H3+ emits in the near-infrared, allowing for the remote exploration of the physical properties of the upper atmosphere. However, the Jovian low-latitude ionosphere remains largely unexplored because H3+ emissions from this region are faint and spectrally entangled with bright neutral species, such as CH4. Here, we present James Webb Space Telescope H3+ observations of Jupiter’s low-latitude ionosphere in the region of the Great Red Spot, showing unexpected small-scale intensity features such as arcs, bands and spots. Our observations may imply that the low-latitude ionosphere of Jupiter is strongly coupled to the lower atmosphere via gravity waves that superimpose to produce this complex and intricate morphology.