This paper presents the research results on the rheological behavior of Polyamide 6 (PA6) film during uniaxial stretching. The creep and relaxation tests of PA6 films were conducted on dynamic mechanical analysis (DMA) tester under uniaxial tensile conditions. And analyzed the PA6 film creep behavior under different stress and temperature, the stress relaxation behavior under different strain. With the increase of temperature or stress, the creep behavior of PA6 film was enhanced. With the increase of strain, the relaxation of PA6 film showed an enhanced tendency. Fractional-order Maxwell model, fractional-order Kelvin model and fractional-order linear body model were used to fit and analyze the experimental data under different conditions. The results showed that the fractional-order linear body model was better suited for describing the rheological behavior of PA6 film under uniaxial stretching. Then, the fractional-order linear body model was used to fit and analyze the creep and relaxation curves under different conditions, and the rheological properties of PA6 film were discussed according to the fitting results. The results indicated that the rheological elements in the internal microstructure of PA6 film were more easily activated under high stress state, and the macroscopic viscous characteristics were more obvious under high strain state. The above results will provide a theoretical basis to further explore the deformation mechanism of PA6 film during tensile tests.