Polyunsaturated Fatty Acids Ratio Research Articles

Life on a piece of cake: performance and fatty acid profiles of black soldier fly larvae fed oilseed by-products

The oilseed crops Crambe abyssinica and Camelina sativa produce oils rich in erucic acid and n-3 polyunsaturated fatty acids (PUFA), respectively. After pressing the oil, a seed cake remains as a protein-rich by-product. Edible insects may convert this seed press cake and the defatted seed meal produced from it into insect biomass suitable for animal feed. Black soldier fly larvae (BSF, Hermetia illucens) can grow on a wide range of organic waste types, but may be hindered by excess protein or the plant toxins characteristic for these two oilseed crops, i.e. glucosinolates and their breakdown products. We tested the effects of 25, 50 and 100% oilseed by-product inclusion in the diet on survival, development, biomass production and fatty acid composition of BSF larvae. Larval performance on diets with up to 50% camelina by-product or 25% crambe by-product was similar to performance on control diet (chicken feed), and decreased with higher inclusion percentages. Larval fatty acid profiles differed significantly among diets, with larvae fed press cake more distinct from control than larvae fed seed meal. Larvae fed camelina press cake had more α-linolenic acid, whereas larvae fed crambe contained most oleic acid. The n-6:n-3 PUFA ratio decreased with increasing proportion of by-product, especially on camelina diets. Lauric acid content was highest in larvae fed 100% camelina meal or 50% crambe meal. These results indicate that BSF larvae can be successfully grown on diets with camelina or crambe oilseed by-products, and that the resulting larval n-6:n-3 PUFA ratio is favourable for animal feed. However, the fate of glucosinolates and their derivatives remains to be determined, to guarantee chemical safety of camelina- or crambe-fed BSF larvae for animal feed.

Serum Ω-6/Ω-3 Polyunsaturated Fatty Acids Ratio and Identification of Diabetic Retinopathy: A Propensity Score Matching Based Case-Control Study in China

Background: Optimal ω-6/ω-3 polyunsaturated fatty acids ratio (PUFAR) is reported to exert protective effects against chronic diseases. However, data on PUFAR and diabetic retinopathy (DR) are scarce. We aimed to thoroughly quantify how PUFAR was associated with DR as well as its role in DR detection. Methods: This is a two-center case-control study, patients were matched using a propensity score algorithm. Multivariable logistic regression models and restricted cubic spline analyses were used to examine the association of PUFAR with DR after adjusting for confounders, which were screened by directed acyclic graph. Performance of the predictive ability to identify DR with PUFAR was assessed by receiver operating characteristic (ROC) analysis and Hosmer-Lemeshow test. Findings: An apparent negative relationship between PUFAR and DR was observed. Adjusted likelihood of DR decreased by 79% (OR:0.21, 95% CI:0.10-0.40) with per interquartile range of PUFAR elevation. Similar results were also obtained in tertile analysis. As compared to those in the 1st tertile of PUFAR, the adjusted odds of DR decreased by 76% (OR:0.24, 95% CI:0.08-0.66) and 93% (OR:0.07, 95% CI:0.03-0.22) for subjects in the 2nd and 3rd tertiles, respectively. Good calibration and discrimination of the predictive model were detected and consistent in both training and testing sets, and PUFAR=35 would be an ideal cut-off value for DR identification. Interpretation: Our present study shows that serum PUAFR was inversely associated with DR and could serve as an ideal biomarker in distinguishing DR from type 2 diabetes. Funding: This study was funded by Natural Science Foundation of Zhejiang Province, Zhejiang Basic Public Welfare Research Project, the Major Project of the Eye Hospital of Wenzhou Medical University and the academician’s science, and technology innovation program in Zhejiang province. Part of this work was also funded by the National Nature Science Foundation of China and Science and Research Funding Project for College Students in Wenzhou Medical University. Declaration of Interest: We declare no conflicts of interests. Ethical Approval: The protocol had been carefully reviewed and approved by the Ethic committee of the Eye hospital of Wenzhou medical university before the study (Number: KYK [2017] 46).

The use of bio-efficient plant materials to optimize the fatty acid composition of poultry terrins

The development of functional products characterized by high biological efficiency is an urgent task. The article presents the results of the development of balanced-fatty acid terrins from poultry meat. To increase the biological effectiveness of chopped products, raw materials containing omega-3 and omega-6 polyunsaturated fatty acids (PUFAs) were selected. Based on the analysis of the chemical composition of the raw material kit, the intervals for the Introduction of ingredients containing biologically effective components are calculated. Their optimum amount has been experimentally established. The resulting innovative products are characterized by a balanced composition in terms of content and ratio of omega-3 and omega-6 PUFAs. In addition, the value of the indicator of biological value has increased. Developed poultry terrins can be recommended both for a wide range of consumers, and for use in diet (including low-carb diets), baby food. The developed terrines will expand the range of chopped poultry products of functional orientation.

Characterization of fatty acids and nutrient composition of SAU Perilla-1 [(Perilla frutescens (L.) Britton] seeds grown with agro climatic conditions in Bangladesh

SAU Perilla-1 (Golden perilla BD) is a newly edible oil seed crop variety introduced by Sher-e-Bangla Agricultural University in Bangladesh. Its seed oil is a rich source of unsaturated fatty acid (91%), of which more than 50% is α-linolenic acid (type of omega-3 fatty acid). The estimated ratio of saturated, monounsaturated and polyunsaturated fatty acids was found 1: 2.26: 8.95. Moreover, the α-linolenic acid was detected (50.52%) as the most dominating polyunsaturated fatty acid, which was 4-four times higher than monounsaturated fatty acids in the oil of the crop variety. Fatty acid analysis of oil revealed a ratio (1:2.22) of Omega 6 to Omega 3 fatty acids, which lies within a healthy range as documented by the global scientific community. Compared to other plant seed oils, SAU Perilla-1 oil consists of Linoleic acid (Omega 6 fatty acid, 22.71%) – a component associated with obesity, which is far below the regular oils from soybean, sunflower and corn. Therefore, our findings indicated that SAU Perilla-1 seed is one of the best edible sources of plant oils rich in essential fatty acids conducive to human health.

Effect of Weather Conditions on the Fatty Acid Composition of Medium-Growth Chicken Reared in Organic Production System

A total of 160 medium-sized one-day-old male chicks reared in organic conditions were studied individually from the first day of their life until slaughter (120 days). Two weather periods were considered, being period C colder than period H. A total of 24 chickens per period were randomly selected, then the breast muscle (m. Pectoralis major) was extracted for analysis. Individual fatty acids were measured by gas chromatography and expressed in grams per 100 g of fat. From the values obtained, total lipid fractions were calculated. Near infrared spectroscopy spectra (NIRS) were recorded on the surface of the breast without manipulating. Breast from chicken reared in H period had significantly lower (p<0.05) saturated fatty acids / polyunsaturated fatty acids (SFA / PUFA) ratio, and increased (p<0.05) content in PUFA and n-6. However, no significant differences were observed on the content of individual fatty acid. NIR system was not able to correctly classify the samples according to the breeding period.

A comparison of meat composition, tenderness and the fatty acid profile of three muscles from Holstein-Friesian bulls from production system resulting in final ages of either &lt;16 or 19 months

Context The increased number of male dairy origin calves in Ireland due to the abolition of European Union milk quotas is a potential resource for the beef industry. Rearing these animals as bulls rather than steers is more efficient from a production perspective. Ensuring satisfactory quality of bull beef from dairy origin is essential. Aim To determine the effect of two production systems and three muscle types on physico-chemical characteristics and fatty acid (FA) profile of beef from Holstein-Friesian (HF) bulls. Methods Thirty HF bulls were equally assigned to two production systems, namely, slaughter at under 16 months of age or slaughter at 19 months of age. Longissimus thoracis (LT), Semitendinosus (ST) and Gluteus medius (GM) muscles were excised post-slaughter for determination of pH, colour, Warner–Bratzler (WB) variables, cook loss, chemical composition [intramuscular fat (IMF), moisture, protein, ash], collagen characteristics and FA profile. Results WB variables and cook loss after 14 days postmortem ageing, and insoluble and total collagen contents were higher, while IMF content, redness and saturation at 24 h post-blooming were lower for muscles from the 19-month production system. Muscles from the under 16-month production system had a higher saturated fatty acid (SFA) proportion and n-6:n-3 polyunsaturated fatty acid (PUFA) ratio, while muscle from the 19-month production system had a higher PUFA proportion, n-3 PUFA proportion and PUFA:SFA ratio. The GM muscle had the lowest L* value, followed by LT and ST. Yellowness, saturation and hue angle were greater in ST. LT had lower WB variables, cook loss, moisture, and a higher IMF content than ST and GM. The PUFA proportion and PUFA:SFA ratio were highest in ST, followed by GM and LT. IMF, total FA, SFA and monounsaturated fatty acid (MUFA) concentrations were higher in LT from the under 16-month production system bulls (the interaction). Conclusions Beef from the under 16-month production system compared with beef from the 19-month production system, and LT compared with ST and GM muscles had better quality characteristics. Implications Dairy bulls can produce beef of acceptable quality. The findings will guide selection of the combination of production system and muscle type most appropriate to specific market requirements.

Trace element content, Amino acid composition and fatty acid profiles and of Timadidite’s lamb meat, raised in an agro-silvo-pastoral system of the Moroccan middle-Atlas

Traditional food products are often considered beneficial and healthy Foods. Timahdite sheep breed is the main sheep breed raised in the middle-Atlas in Morocco. The breeding of this local rustic breed plays a crucial socio-economic role in this mountain area. Indeed, it generates a significant economic input for the rural population and plays a role of valuing natural resources in forest areas. Little information is available on Timahdite sheep meat produced in a traditional production system. This study aimed to provide more insights on the quality and nutritional value of Tamahdite’s sheep meat, which is in the process of being labeled “Protected Geographical Indication” as a protected product from the Middle Atlas. The longissimus lumborum is used for meat analysis and quality parameter determination. Analyses of amino acid composition show that this meat has a high protein value with a protein digestibility-corrected amino acid score of 325.09 and EAA index of 162.20. The fatty acid profile analysis shows that this meat has polyunsaturated fatty acids (PUFA)/saturated fatty acids and n-6/n-3 PUFA ratios of 0.50 and 9.60, respectively. In addition, the results show that this meat contains 5.70 mg/100g of trace elements, where zinc and iron are the most abundant.

Adherence to the Mediterranean diet and body composition of breast-feeding mothers: the potential role of unsaturated fatty acids.

A paucity of evidence is available regarding the impact of diet's quality during pregnancy and lactation on the body composition of breast-feeding mothers. The purpose of the present study was to evaluate the association between maternal degree of adherence to the Mediterranean diet (MD) and body composition measures specifically those relating to body fat, in the lactation period. A cross-sectional study on healthy mothers of full-term babies has been conducted. At 30 ± 10 d after delivery, anthropometric measurements and body composition were assessed. A food frequency questionnaire was performed to compute the Italian Mediterranean Index (IMI) score as an index of adherence to the MD. Data related to pregnancy such as pre-pregnancy weight, gestational weight gain and morbidities were also collected. The 147 mothers included were categorised in IMI-1 (IMI score < 5; n 92) and IMI-2 (IMI score ≥ 5; n 55) groups. IMI-2 mothers showed higher daily energy, total carbohydrates, starch and fibre intakes than IMI-1. The dietary habits of IMI-2 mothers reflect the typical characteristics of MD: they consumed higher quantities of proteins and lipids of vegetal origin, higher amounts of monounsaturated and polyunsaturated fatty acids (PUFAs) and lower saturated to PUFAs ratio. The IMI-2 group showed lower absolute fat mass and fat mass index compared to IMI-1 [(20⋅2 ± 5⋅9) v. (22⋅9 ± 8⋅4) kg; P 0⋅036 and (7⋅5 ± 2⋅2) v. (8⋅5 ± 3⋅1) kg/m2; P 0⋅036, respectively], whereas body weight [(61⋅1 ± 8⋅0) v. (63⋅3 ± 9⋅2) kg] and body mass index [(22⋅4 ± 2⋅6) v. (23⋅3 ± 3⋅5) kg/m2] were similar. The degree of adherence to the MD during pregnancy and lactation is positively associated with lower maternal fat deposition in the breast-feeding period. The higher quality of dietary lipids, probably in synergy with the assumption of starchy carbohydrates and fibre, could influence maternal body fat.

ORGANIC PRODUCT WITH BALANCED COMPOSITION OF ω-6 and ω-3 FATTY ACIDS

The article presents the results of the development of blended vegetable oilwith the optimal ratio of polyunsaturated fatty acids from cold-pressed vegetableoils: sunflower, flaxseed, pumpkin. As a result of the experiments, an oilcomposition was obtained with a polyunsaturated fatty acid ratio of ω-6 and ω-3 of5:1, with a percentage of 81% sunflower, 12% linseed and 7% pumpkin oil. Theresulting organic product is designed for daily consumption to correct thedeficiency of essential fatty acids in the diet. The results of the analysis of thelimiting values of the characteristics of hydrolytic and oxidative damage - the acidand peroxide numbers during the storage of finished products for 12 months, it wasshown that the excess of these indicators over the norm begins with a period ofmore than 8 months. So, the recommended shelf life of the blended oil is 6 months,at a temperature of 20-25°C.

Impacts of fat types and myofibrillar protein on the rheological properties and thermal stability of meat emulsion systems

Studies have shown the effects of fat or oil types and myofibrillar protein on meat emulsions. In this study, fat extracted from pork, beef, chicken, and duck, as well as corn oil, was used to emulsify the extracted porcine myofibrillar protein. We evaluated the thermal and rheological properties, emulsion stability, texture profiles, fatty acid compositions, and microstructures of these meat emulsions. Meat emulsions containing animal fat had lower emulsion stability and better thermal stability, rheological properties, and hardness than those containing oil. The ratio of polyunsaturated fatty acids in the meat emulsion containing corn oil was the highest, followed by duck, chicken, pork, and beef fat emulsions. Of the animal fat emulsions, chicken might be the best fat source when emulsifying porcine protein because of the high thermal and emulsion stability, rheological properties, and fatty acid composition of the emulsion and well-distributed fat particles in it.

Association Between Dietary Fish and PUFA Intake in Midlife and Dementia in Later Life: The JPHC Saku Mental Health Study.

The relationship between midlife dietary habits and risk of dementia remains unclear. To investigate the association between dietary fish and n-3 polyunsaturated fatty acid (PUFA) consumption in midlife and risk of dementia in later life. This population-based cohort study assessed food frequency (average intake in 1995 and 2000) and cognition (2014-2015) in 1,127 participants (aged 45-64 in 1995). We used logistic regression analyses to calculate odds ratios (ORs) for dementia and mild cognitive impairment (MCI) diagnoses for consumption quartiles of fish, PUFA-rich fish, total n-3 PUFAs, total n-6 PUFAs, types of PUFAs, and n-3/n-6 PUFA ratio. Estimated ORs were adjusted for age; sex; education; smoking status; alcohol consumption frequency; physical activity; histories of cancer, myocardial infarction, and diabetes mellitus; and depression. Significantly reduced risks of dementia over non-dementia (MCI plus cognitively normal) were observed in the second (OR = 0.43 (95% CI = 0.20-0.93)), third (OR = 0.22 (95% CI = 0.09-0.54)), and highest quartiles (OR = 0.39 (95% CI = 0.18-0.86)) for fish; the third (OR = 0.39 (95% CI = 0.16-0.92)) and highest quartiles (OR = 0.44 (95% CI = 0.19-0.98)) for eicosapentaenoic acid (EPA); the second (OR = 0.39 (95% CI = 0.18-0.84)), third (OR = 0.30 (95% CI = 0.13-0.70)), and highest quartiles (OR = 0.28 (95% CI = 0.12-0.66)) for docosahexaenoic acid (DHA); and the third (OR = 0.36 (95% CI = 0.16-0.85)) and highest quartiles (OR = 0.42 (95% CI = 0.19-0.95)) for docosapentaenoic acid (DPA). High intake of fish in midlife might aid in preventing dementia.

Proximate Chemical Composition, Fatty Acid Profile, and Lipid Qualitative Indices of Brown Bear Meat.

Although game meat quality has been under the spotlight in numerous studies, the quality of brown bear (Ursus arctos) meat is still unknown. The aim of this study was to determine the effects of sex and age on the proximate chemical composition, fatty acid profile, and lipid indices of brown bear meat. Nine (n = 9) females and nine (n = 9) males were hunted during the Croatian spring hunting period in 2018. Based on age, bears were divided into two groups: <3 years (n = 9; five females and four males) and 4–6 years (n = 9; four females and five males). For analysis purposes, samples of M. semimembranosus were collected. Age was shown to have an effect on the traits analyzed, while sex-related differences were not found. Brown bear meat has a high fat content (average 6.12%), especially in older bears (~9%). The contents of protein, dry matter, and ash were similar to those of other game species. Monounsaturated fatty acids made up approximately 50% of all fatty acids, with the most abundant being C18:1n-9. More favorable profiles of essential polyunsaturated fatty acids were found in younger bears. The ratio of polyunsaturated and saturated fatty acids was closer to the recommended ratio than the ratio of n-6 and n-3 polyunsaturated fatty acids, and lipid indices were favorable. Further research is needed to determine seasonal changes in brown bear meat quality.

Decreased plasma n6:n3 polyunsaturated fatty acids ratio interacting with high C-peptide promotes non-alcoholic fatty liver disease in type2 diabetes patients.

Aims/IntroductionTo explore relationships between polyunsaturated fatty acids (PUFA) and non‐alcoholic fatty liver disease (NAFLD) in patients with type 2 diabetes, and whether insulin action has an interactive effect with PUFA on NAFLD progression.Materials and MethodsWe extracted clinical and omics data of 482 type 2 diabetes patients from a tertiary hospital consecutively from April 2018 to April 2019. NAFLD was estimated by ultrasound at admission. Plasma fasting n3 and n6 fatty acids were quantified by liquid chromatography–tandem mass spectrometry analysis. Restricted cubic spline nested in binary logistic regression was used to select the cut‐off point, and estimate odds ratios and 95% confidence intervals. Additive interactions of the n6 : n3 ratio with insulin action for NAFLD were estimated using relative excess risk due to interaction, attributable proportion due to interaction and synergy index. Relative excess risk due to interaction >0, attributable proportion due to interaction >0 or synergy index >1 indicates biological interaction. Spearman correlation analysis was used to obtain partial correlation coefficients between PUFA and hallmarks of NAFLD.ResultsOf 482 patients, 313 were with and 169 were without NAFLD. N3 ≥800 and n6 PUFA ≥8,100 μmol/L were independently associated with increased NAFLD risk; n6 : n3 ratio ≤10 was associated with NAFLD (odds ratio 1.80, 95% confidence interval 1.20–2.71), and the effect size was amplified by high C‐peptide (odds ratio 8.89, 95% confidence interval 4.48–17.7) with significant interaction. The additive interaction of the n6 : n3 ratio and fasting insulin was not significant.ConclusionDecreased n6 : n3 ratio was associated with increased NAFLD risk in type 2 diabetes patients, and the effect was only significant and amplified when there was the co‐presence of high C‐peptide.

Fatty acids, health lipid indices, and cholesterol content of sheep meat of three breeds from Moroccan pastures.

The lipid analysis of commercial lamb's meat, from two main Moroccan production areas Middle Atlas and highlands of eastern Morocco, was conducted. This study concerns the lipid quality of sheep meat from Beni Guil (BG) and Timahdite (Ti) as indigenous breeds and that of the Ouled Djellal (ODj) breed of Algerian origin. To study the effect of the geographical area, the meat samples from the Beni Guil breed were taken in the two main locations of this breed: in the region of Ain Beni Mathar (BGA) and that of Tendrara (BGT). The fatty acid profiles of the sheep meats analyzed showed the following: (i) polyunsaturated fatty acid (PUFA) richness was of 12.87 % and 20.59 % respectively for BGA and BGT breeds; (ii) polyunsaturated saturated fatty acid ratios were ranged between 0.28 to 0.50 respectively for BGA and Ti breed; and (iii) PUFA- content was 1.71 % for ODj breed and 2.13 % for BGA. Thus, the PUFA- PUFA ratios range between 4.92 and 9.6 for BGA and Ti sheep meat, respectively. The cholesterol content was 0.08 % and 0.12 % of fresh meat respectively for ODj and BGA. Finally, meats of BGA and ODj have similar thrombogenicity (1.23 and 1.27 respectively) and atherogenicity indices (0.71 and 0.68 respectively). Statistically, these values were significantly higher than those registered for Ti breed (IT: 1.04 and AI: 0.51). In conclusion, from a nutritional point of view, it can be deduced that these meats have an interesting lipid quality due to their richness in desirable fatty acid (UFA C18 : 0).

The effects of cooking salmon sous-vide on its antithrombotic properties, lipid profile and sensory characteristics

Fish contains bioactive polar lipids (PL) and is mainly consumed cooked. The aim of this study was to evaluate the sensory characteristics of sous-vide cooked salmon and the in vitro cardio-protective properties of its PL. PL were extracted from brined and un-brined sous-vide preparations in 52 °C, 65 °C, and 80 °C, while their antithrombotic cardio-protective properties were assessed in human platelets and their fatty acid (FA) content was evaluated by LC-MS. Sensory tests were performed using napping followed by check-all-that apply (CATA). Mild temperatures (52 °C, 65 °C) did not affect the inhibitory effect of PL from brined and un-brined salmon, against human platelet aggregation induced by platelet-activating factor (PAF), thrombin, adenosine diphosphate (ADP) or collagen. In higher temperatures used for pasteurization (80 °C), a reduction of antithrombotic properties was observed in PL from both un-brined and brined salmon samples. This reduction was accompanied by a decrease of their n3 eicosapentaenoic acid (EPA) and overall polyunsaturated FA (PUFA) content, but only in the PL of the un-brined salmon preparations. Thus, changes in the fatty acid content of PL of all sous vide salmon preparations, and especially of specific PUFA, seem to be associated with the observed changes in their antithrombotic potency. Changes in the content of the n-3 docosapentaenoic acid (DPA), a precursor of EPA and docosahexaenoic acid (DHA), seem to be associated with differences observed in the antithrombotic potency of PL from different sous vide salmon preparations. Taste attributes were not affected by the conditions of sous-vide preparations, whereas slight textural differences were observed in samples treated at 65 °C and 80 °C. These outcomes, if combined with the observed low values of the n-6/n-3 PUFA ratio in PL of all sous-vide preparations, further suggest a beneficial role for such a mild cooking procedure for preserving the antithrombotic and cardio-protective properties of salmon without affecting its sensory characteristics.

CONSUMER VALUE OF FRESHWATER FISH JELLIES

Background. In order to form a balanced diet that ensures harmonious deve­lopment of human, prevention of various diseases, especially in conditions of coronavirus infection COVID-19 the recipes for jellied products based on freshwater fish with the addition of plant additives (cranberries, parsley roots, carrots, beets, fresh green and onions, spicy-flavored vegetables (green dill, parsley) and seaweedweredeveloped. The aim of the work is to study the consumer value of jellied fish products with herbal supplements. Materials and methods. The object of research is freshwater fish jellies with the addition of plant additives and seaweed. Jellied fish without vegetable additives was selected as a control sample. The content of moisture, ash, fat, protein, amino acid composition of proteins, fatty acid composition of lipids, mineral composition; the content of vitamins (thiamine, riboflavin, niacin, carotenoids, tocopherols) was studied by conventional methods. Repea­tability of experiments –five times, the relative error did not exceed 5 %. Results. Studies of the chemical composition show that the control sample, in contrast to the studied ones, contains more fat and protein, which is explained by the lack of vegetable raw materials according to the recipe. However, the addition of vegetable raw materials to the latter increases the mineral content by 15–31 %. The content of essential amino acids in the control is 45 % of the total content, and in the test samples – 46 %. The dominant amino acid in the samples is lysine, and the limited ones are valine and isoleucine. The utilitarian coefficient of the amino acid composition of the studied samples (0.61–0.63) indicates a high balance of amino acids relative to the standard. The low coefficient of redundancy (0.21–0.23) and the high coefficient of utilitarianism indicate the maximum degree of assimilation of proteins of jellied fish products by the human body. The content of polyunsaturated fatty acids (PUFA) in the filling products was 26 %. The ratio of PUFA: SFA, C18:2: C18:1 and C18:2: C18:3 is established as rational in jellied fish. The value of the absorption coefficient for the studied samples of jellied products with beets, onions and control is 0.80, and carrots and cranberries – 0.79. The content of thiamine in jellied fish with carrots, onions, beets is 2 times higher than in the control, the content of niacin – 1.2 times, vitamin E – 1.7–1.2 times. The highest content of carotenoids is found in jellied fish with carrots and onions. An increase in the level of Potassium, Calcium, Bromine, Iodine, Iron, Selenium in the experimental filling products compared with the control was determined: Potassium by 45 %,Selenium 5.5–8.0 times, Manganese 1.6 times. Conclusion. The enrichment of fish products with plant components contributes to the expansion of the range of products of high nutritional and biological value with a high degree of satisfaction of the physiological needs of the human body with biolo­gically active substances. Accordingly, the consumption of jellied fish products allows to improve the functional state of the most important systems of the human body, preven­tion of food-dependent diseases, various forms of immunodeficiency, especially in condi­tions of coronavirus infection COVID-19.

Is There an Advantage in Enriching Parenteral Lipid Emulsions Containing Fatty Acids From Fish Oil With Medium-Chain Triglycerides? A Study on Body Pool Concentrations of ω-3 Fatty Acids in Lewis Rats.

The addition of medium-chain triglycerides (MCTs) into parenteral lipid emulsions rich in fatty acids from fish oil (FOLEs) has been shown to improve their clearance and extrahepatic uptake. We assessed whether this effect could favor the leukocyte uptake of ω-3 polyunsaturated fatty acids (PUFAs) for immunomodulatory purposes METHODS: Following 5-day adaptation in metabolic cages, 42 male Lewis rats fed with AIN-93M chow were killed (baseline control group [BC]) or submitted to central venous catheterization and distributed into (1) surgical control group without parenteral infusion (chow group), (2) test emulsion (MCT/LCT/FO) group with the parenteral infusion of a FOLE containing 40% MCT, and (3) control emulsion group (LCT/FO) with the parenteral infusion of an FOLE without MCT. The 2 FOLEs had similar ω-3 PUFA contents and ω-6/ω-3 PUFA ratios and were infused during 48 and 72 hours. Concentrations of ω-3 and ω-6 PUFAs in plasma, liver, and blood mononuclear and polymorphonuclear leukocytes were assessed by gas chromatography RESULTS: In both FOLE groups, leukocyte concentrations of ω-3 PUFAs peaked after 48 hours' infusion (vs BC). At this time point, plasma concentrations of ω-3 PUFAs were higher in MCT/LCT/FO group than in LCT/FO group and the opposite was found in the liver (P<.05), but no differences in PUFA concentrations were observed between these groups in leukocytes (P>.05) CONCLUSION: The ω-3 PUFAs provided by FOLEs rich in MCT were less incorporated by liver and remained more available for extrahepatic cell delivery, but this did not result in a clear benefit in increasing their incorporation by peripheral leukocytes.

Influence of roasting on antioxidants, fatty acids, sensory properties and oxidative stability of macadamia nuts

Raw and roasted kernels of ‘A4’ and ‘Beaumont’ macadamia cultivars were assessed for biochemical and sensory quality during the accelerated storage of 70 days. Kernels were roasted at 125 °C for 15 min using a hot air oven dryer. Roasted kernels had significantly (p < 0.001) high concentration of phenols, antioxidants and good sensory properties: aroma, taste and overall appearance, validated by low levels of polyphenol oxidase (PPO), peroxide value (PV) and fatty acids (FAs) at the end of storage. Whereas, raw kernels had high and unacceptable levels of PPO, PV and FA compositions which accelerated the rancidification process, supported by low concentration of phenols, antioxidants and poor aroma. A strong correlations were observesd between ratio of polyunsaturated FA: monounsaturated FA (PUFA:MUFA) and sensory quality attributes (aroma and appearance) for roasted cultivars before storage. And also positive correlation was observed among PV, rancidity and cultivars which indicate that raw ‘Beaumont’ and roasted ‘A4’ macadamia nuts after 70 days of storage had higher PV values, resulting to rancidity taste. Roasting can alter fatty acid profile of the nuts depending on the roasing temperature. These findings demonstrate that roasting can improve macadamia nuts’ quality as compared to raw nuts, but the roasting temperature regime needs to be determined for different cultivars.

Administration of an Intravenous Fat Emulsion Enriched with Medium-Chain Triglyceride/ω-3 Fatty Acids is Beneficial Towards Anti-Inflammatory Related Fatty Acid Profile in Preterm Neonates: A Randomized, Double-Blind Clinical Trial.

Intravenous administration of pure soybean oil emulsions high in linoleic acid may lead to inflammation and lipid peroxidation in preterm neonates. We aimed to investigate the effects of a medium-chain triglyceride (MCT)/ω-3 polyunsaturated fatty acid (PUFA)-enriched intravenous fat emulsion (IVFE) on plasma fatty acid (FA) profile and serum interleukin-6 (IL-6) in preterm neonates. In this double-blind randomized study, 92 preterm neonates (gestational age < 32 weeks, birth weight < 1500 g) were assigned to receive either MCT/ω-3 PUFA-enriched IVFE (Intervention Group) or soybean oil-based IVFE (Control Group). Levels of FAs were measured at baseline (day 0) and day 15 of parenteral nutrition with gas-chromatography mass-spectrometry. Serum IL-6 was measured with sandwich ELISA in 59 neonates. Plasma FAs changed significantly over time; the MCT/ω-3 PUFA-IVFE group showed higher ω-3 PUFAs (p = 0.031), eicosapentaenoic acid (p = 0.000), and oleic acid (p = 0.003), and lower ω-6/ω-3 PUFAs ratio (p = 0.001) and ω-6 PUFAs (p = 0.023) compared to control group. Linoleic acid was higher in the soybean oil (SO)-based IVFE arm compared to the MCT/ω-3 PUFAs-IVFE arm (p = 0.006). Both fat emulsion types decreased IL-6 compared to baseline, but changes were insignificant between groups. Administration of MCT/ω-3 PUFA-enriched IVFE in preterm neonates is beneficial in changing the FA profile consistent with attenuated inflammatory response.

Effects of Low ω6:ω3 Ratio in Sow Diet and Seaweed Supplement in Piglet Diet on Performance, Colostrum and Milk Fatty Acid Profiles, and Oxidative Status.

Simple SummaryFeeding maternal animals divergent ratios of omega-6 (ω6) and omega-3 (ω3) fatty acids can change not only their health, physiological condition, and performance but also do the same for their offspring. In swine production, various ω6:ω3 ratios have been tested, but the search for an optimal proportion in the sow diet is still in progress. For piglets, weaning oxidative stress has been alleviated by supplementing with abundant sources of bioactive compounds. In this case, brown seaweed, a rich source of natural antimicrobials and antioxidants, can be a good candidate, but its supplementation in piglet diet is limited. This study explores the hypothesis that feeding a low ω6:ω3 ratio diet to sows during gestation and lactation, together with the supplementation of Ascophyllum nodosum for piglets during the post-weaning period, could benefit piglets’ performance and oxidative status more than the respective single treatment provided to the mother or the piglet. Results showed that the low dietary ω6:ω3 ratio (4:1) and seaweed supplement did not affect the post-weaning piglets’ growth rate and oxidative status. However, a low ω6:ω3 ratio diet alone improved weaning survival rate, suckling piglets’ weight gain, and total ω3 fatty acids in colostrum and milk.The ratio of omega-6 (ω6) to omega-3 (ω3) polyunsaturated fatty acids (PUFAs) in the diet contributes to animal health and performance modulations because they have mostly opposite physiological functions. Increasing ω3 PUFAs content in the maternal diet can stimulate antioxidative capacity in sow and piglets; however, the optimal ratio of ω6 and ω3 PUFAs in the sow diet is still under discussion. Rich sources of bioactive constituents such as brown seaweed are an excellent supplementation to promote animal health and antioxidant status. However, the knowledge of the effects of this compound, specifically in post-weaning piglets, is still limited. Moreover, the combined effect of a low ω6:ω3 PUFAs ratio in sow diet and seaweed supplementation in post-weaning piglets’ diet has never been studied. This research aims to assess the combined effect of a low ω6:ω3 ratio in sow diets and seaweed supplementation in piglet diets on their growth and oxidative status. We also assessed the impact of a low ω6:ω3 ratio in the maternal diet on reproduction, milk fatty acid (FA) profile, and plasma leptin concentration. Two sow diets (n = 8 each) contained either a control ratio (CR, 13:1 during gestation, starting from day 28 (G28) and 10:1 during lactation) or a low ratio (LR, 4:1 from G28 until the end of lactation (L-End)) of ω6:ω3 FA by adding soybean oil or linseed oil, respectively. Reproductive performance was evaluated. Colostrum and milk at lactation day 7 (L7) and L-End were collected to analyze FA profile. Plasma was collected at G28, G79, G108, L7, L14, and L-End for determination of leptin and oxidative status. At weaning, 20 male piglets were selected per sow group to form 4 diet treatments (n = 10 each), which were supplemented with or without 4 g/kg seaweed. Recording of growth performance and collection of blood were performed at days 0, 7, 15, and 21 of post-weaning for oxidative status. LR diet increased (p < 0.05) the survival rate of piglets at weaning, and individual and litter weight gains. Colostrum and milk at L7 and L-End had lower (p < 0.05) ω6:ω3 ratio in LR sows. Interaction between dietary treatments on sows and piglets was revealed for all examined growth parameters at most time points (p < 0.05). LR diet did not affect plasma leptin levels and oxidative status. These findings suggest that the seaweed supplement during post-weaning could not improve growth rate and oxidative status of piglets born from mothers receiving a low dietary ω6:ω3 ratio (4:1) during gestation and lactation. However, this low ratio was beneficial for weaning survival rate, sucking piglets’ weight gain, and ω3 enrichment in colostrum and milk.