Polyherbal Cream Research Articles

Paronychia Treatment: Formulation and Evaluation of Polyherbal Cream

Paronychia Treatment: Formulation and Evaluation of Polyherbal Cream

Formulation and evaluation of polyherbal cream using Murraya koengii, Nyctanthus arbortristis and Ixora coccinea leaves hydro-alcoholic extract

Formulation and evaluation of polyherbal cream using Murraya koengii, Nyctanthus arbortristis and Ixora coccinea leaves hydro-alcoholic extract

Formulation and Evaluation of Polyherbal Cream

Herbal skin creams are mainly intended for beautification of skin. The main aim of the present research work is to prepare a face cream using different herbs and the prepared cream is evaluated for stability and anti bacterial activity. The herbs used in the formulation (F1, F2, and F3) are flowers of Clitoria ternatea, leaves of Azadirachta indica and Ocimum sanctum. The formulated cream is evaluated for the various parameters like organoleptic properties, pH, stability, consistency, skin irritation, and antibacterial properties. F1 formulation has shown good stability and antibacterial properties compared to marketed cream.

Evaluation of Wound Healing Activity of Polyherbal Skin Care Cream

Plants have long history of use to cure and prevention of various human ailments. In the present study Azadirachta indica, Ocimum sanctum, Centella asiatica and Hibiscus rosa sinensis extracts have been used to formulate skin care cream with varied proportion of individual extract. The formulations F3 and F4 have been selected for evaluation of wound healing study on the basis of preliminary evaluation results of parameter evaluated for Polyherbal cream. The formulation F3 and F4 were compared with negative control and standard treated group. It was found that the formulation F4 has greater wound contraction and efficiently reduced the time of epithelization campared to negative control with significant level P<0.050 and P>0.001, results indicated that the formulation F4 could be used as effective agent in wound healing.
 Keywords: Herbal cream, Wound healing, Flavonoids, Phytochemical

Evaluation of Methanolic Extract of Polyherbal Cream on DNCB-induced Atopic Dermatitis in Wistar Albino Rats

Evaluation of Methanolic Extract of Polyherbal Cream on DNCB-induced Atopic Dermatitis in Wistar Albino Rats

Formulation and Evaluation of Polyherbal Cream

Cosmetics made from either natural or synthetic components are almost in regular use universally in many different forms for enhancing the beauty. Azadirachta indica and Nyctanthes arbor-tristis are medicinal plants; these plants are used traditionally from ancient years in various herbal medicinal systems such as Ayurvedic, Homeopathic and Siddha. The dried leaves of Azadirachta indica and dried leaves of Nyctanthes arbor-tristis have antibacterial, anti-inflammatory analgesic activity. The present research is focused on the formulation of polyherbal cream and their evaluation by using various evaluation parameters.
 Keywords: Polyherbal cream, Neem, Night Jasmine, Fusion method, Almond oil.

Formulation and Evaluation of Polyherbal Anti-Aging Cream of Clitoria Ternatea, Mangifera Indica and Annona Squamosa

The main objective of the present study is to formulate the polyherbal antiaging cream and evaluation of various parameters of the cream. The evaluation parameters consist of phytochemical evaluation, physiological evaluation, stability studies, homogeneity, appearance, ph, etc. The polyherbal cream was formulated on basis of antioxidant activity of selected plant extracts. The cream was formulated by using natural herbal ingredients like Clitoria Ternatea (Butterfly pea), Mangifera indica (mango), and Annona squamosa (Custard Apple). Extraction of Mangifera indica and Annona Squamosa was carried out by cold maceration where Ethanol and distilled water used as solvent (Hydroalcoholic extraction). Extraction of Clitoria Ternatea was carried out by hot water extraction by using distilled water as a solvent. Phytochemical screening all the three extracts shows presence of flavonoids, tannins, alkaloids, and phenols. Formulated cream was evaluated by using different parameters such as pH, Appearance, Spreadability, Washability, Irritancy test, Stability Studies, etc. There is no evidence of phase separation and final formulation do not show rashes or redness, edema on skin. These study was suggest that different composition of all three extracts and base used in the cream are more stable and safe. It can be concluded from present study that herbal cream having an antioxidant activity without any side effect and can be used as a provision to barrier of skin and to avoid skin aging.

Development of Poly Herbal Cream Formulations Containing Wild Growing Omani Medicinal Plants

Acne is derived from the Greek word “acme” which means “prime of life”. It is mostly considered to be benign and self-limiting condition but if it is ignored than it can progress to lifetime presence and scaring of the skin. Almost 85% of adolescence are affected by acne and from here males are at higher ration that female due to testosterone level that causes increase size in sebaceous gland which in turn causes more production of sebum (oil production). Women are affected more during adulthood due to imbalance of premenstrual hormones and over use of oil based cosmetics. There are many different factors that contribute to acne formation and it has many different forms. Herbal medication are considered safer than allopathic medicines because allopathic medicines are associated with various side effects such as like contact allergy, local irritation, scaling, photosensitivity, itching, redness, skin peeling, necrosis of the skin etc. In this study there are 3 herbal plants were used to prepare cream formulation that were used to investigate the anti-acne property. The aim of this research work was to develop formulations and to perform its evaluation as an herbal anti-acne cream. The objective of this study was to focus on the ant-acne or anti-bacterial effect of the formulated polyherbal cream containing three plants, Thyme, Aloe Vera and Basil. The results showed that the formulation 10 showed potential anti-acne property had that twice more concentration of aloe Vera than basil and thyme.

Formulation and organoleptic evaluation of Poly Herbal Cream of Punica, Neem, Carrot & Jamun as Active Ingredients

Assuming that herbal preparation is better with fewer side effects than synthetics, natural treatments are more effective than allopathy in terms of side effects for better human body healing. Herbal products have a growing demand in the world market, and the plants have been reported in the literature as having various pharmacological activities such as anti-microbial, anti-oxidant, anti-inflammatory activity, anti-cancer, anti-diabetic. The purpose of this study was to develop anti-aging poly-herbal cream by mixing the extract of Punica leaf, Neem Oil, Jamun powder, Carrot powder as the main ingredient, and then creams were developed based on the anti-oxidant ability of herbal extracts and performed their evaluation study. Punica granatam leaves were shade dried and extracted using the Soxhlet method with different solvents such as n-hexane, benzene, and alcohol. Fine extract powder was collected and removed distilled water thoroughly. The cream was formulated into different concentrations, namely F1, F2, F3, and F4. Similar types of research with similar components have been reported, but in this experiment, the formulation is different, and this work is kept cost-efficient and straightforward; it's an attempt to reduce few components and prepare cream and evaluate its potential. According to The International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use ICH guidelines, the cream was stable during stability studies, and F3 turned out to be a better formulation than the other three.

FORMULATION AND EVALUATION OF POLY HERBAL ANTI AGING FACE CREAMS

Herbal cosmetics are defined as beauty products which possess desirable physiological movement like recuperating, smoothing appearance, improving and molding properties due to the natural fixing.. Herbal Cream is a polyherbal plan that comprises of the concentrates of Aloe barbadensis, Ocimum sanctum, Azadirachta indica and Curcuma longa, Cedro oil, Myristica fragrans, Olium rosae (Rose Oil), Orange Oil, Daucus carota, Prunus dulcis and Ocimum sanctum.. Herbs such as aloe vera leaf, turmeric, banana, neem, papaya, cucumber and other plants are used as herbs in cosmetics. In the current exploration article, the polyherbal face cream is ready by utilizing following fixing that is concentrate of Curcuma longa, Solanum lycoperisum, Carica papaya, Daucus carota, rose oil, olive oil, almond oil, refined water. Solanum lycoperisum is utilized as an enemy of oxidant and forestalls the skin maturing.Olive oil moistures the skin, papaya nourishes the skin and curcumin acts as an antimicrobial agent. The skin maturing is essentially brought about by annihilation of collagen layer in skin which can be forestalled by utilizing almond oil. The pre-arranged polyherbal cream was assessed for the pH, spreadability, homogeneity, thickness, extrudability, invitro dissemination examines, color test, expulsion test, skin bothering and antimicrobial investigations. Among every one of the pre-arranged creams F2 and F3 is discovered to be best details as contrast with another. The total percent drug discharge from the pre-arranged polyherbal cream is discovered to be the 93%.

Optimization And Evaluation Of Polyherbal Topical Cream

Herbal plants and their combination report therapeutic as a well synergistic effect that has been recognized in medicine. So, taking into account this factor, the polyherbal topical cream formulation was prepared by using plant extracts to improve patient compliance, enhance antimicrobial spectrum and enhance aesthetic properties. The study focused on the topical polyherbal cream formulation for delivery of the active constituents present in plants to improve skin diseases. The plant extracts of Ocimum sanctum (OS), Rubia cordifolia (RC), Glycyrrhiza glabra (GG) were utilized for the preparation of cream. The formulated cream was subjected to different evaluation parameters and the results depicted that the spreadability of the formulation was low (17.80 ± 1.10g. cm/sec) and that indicates trouble-free spreading, free from grittiness. In rheological properties all the cream formulations also exhibited the same non-Newtonian behaviour. Polyherbal topical cream showed potential antimicrobial activity against all selected microorganisms. Polyherbal topical cream (PHC-5) was ideal in terms of viscosity than other formulations and showed good drug release. Thus, the formulated polyherbal cream was found to be stable in terms of all physicochemical properties.

Antimicrobial activity of a Polyherbal formulation containing Ocimum sanctum, Rubia cordifolia and Glycyrrhiza glabra extracts against different microbial species

In the present investigation, antimicrobial evaluation of individual creams of Ocimum sanctum, Rubia cordifolia, Glycyrrhiza glabra and their optimized polyherbal cream was carried out using commonly used gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) gram negetive bacteria (Escherichia coli and Pseudomonas aeruginosa and fungi (Candida albicans and Aspergillus niger). The study reported that, both individual extract creams and polyherbal creams have shown antimicrobial activity. The Ocimum sanctum extract based cream was more effective against gram positive bacteria having more zone of inhibition (20±0.57, 15±0.05) than rest 04 species while Glycyrrhiza glabra extract based cream was more effective against gram negative bacteria having more zone of inhibition (20±1.0, 11±0.46) and fungi (10±0.46, 13±0.15). The Rubia cordifolia extract based cream reported to be less stronger action against all species as compared to above mentioned both the creams. Howevere, all the three creams were less potent than reference standard cream. The optimized polyherbal formulation showed enhanced zone of inhibition significantly, compared to individual extract based creams. The effect was even more than the reference standard cream against gram positive (22±0.28, 18±0.20) and gram negative (19±0.15, 22±0.35) bacteria, suggesting synergistic combination of ingredients. Thus study validated the usefulness of polyherbal cream consisting of Ocimum sanctum, Glycyrrhiza glabra and Rubia cordifolia against most common pathogens in dermatology, suggesting possible role to treat multidrug resistant mixed infections. This action may be attributed to synergistic actions of various phytoconstituents involved.

Development and optimization of antioxidant polyherbal cream using artificial neural network aided response surface methodology

Artificial Neural Networks, has become phenomenal in drug modeling, in predicting experimental outcomes and in optimization studies during product development. This research seeks to use artificial neural networks (ANN) to optimize a novel formulation of Polyherbal face cream composed of Cymbopogon citratus, Hibiscus sabdariffa, and Ocimum gratissimum extracts. Central composite design was used to develop a framework for the products to be studied delineating the independent variables to be concentration of oil phase, and that of the emulsifying agents used in formulation. The dependent variables/responses are viscosity, spreadability index, and particle size of the cream. The responses gotten were then optimized using ANN models. The validity of the statistical models used for predicting the observed responses were confirmed by carrying out three confirmation experimental runs at the identified optimum conditions. The viscosity of the formulated cream decreased with increase in the amount of oil phase. However, there was a stronger correlation with amount of emulsifying phase and viscosity of the cream as increased in emulsifier product. The particle sizes did not vary greatly among the various formulations irrespective of the concentration of plant extract. Formulation of Polyherbal face cream composed of Cymbopogon citratus, Hibiscus sabdariffa, and Ocimum gratissimum extracts were shown to have significant antioxidant activity. Using ANN modeling, a prediction of an optimized formulation was made and was shown to have comparable results with the predicted values.

FORMULATION AND EVALUATION OF POLYHERBAL CREAM

Objective: Eugenia caryophyllus, Zingiber officinale and Nyctanthes arbor-tristis are medicinal plants; these plants are used traditionally from ancient year in the various herbal medicinal system such as Ayurvedic, Homeopathic and Siddha. The clove buds of Eugenia caryophyllus, rhizomes of Zingiber officinale and dried leaves of Nyctanthes arbor-tristis have anti-inflammatory analgesic activity. The present research was focus on the formulation of polyherbal cream and their evaluation by using various evaluation parameters of the present research are to formulate polyherbal cream and to evaluate the polyherbal cream.
 Methods: the slab method was used for the preparation of cream.
 Results: The evaluation parameters are coming under this heading physical parameter like color was slightly white green, the odor was characteristics, consistency was smooth and the state was semisolid. PH of the cream was 6.5; Spreadability was 7.4g. cm/sec time required for this test was 15 sec, Washability was easily washable, the cream was nonirritant, viscosity of the formulated cream was 39010 cps and no phase separation was observed during storage of polyherbal cream.
 Conclusion: This cream formulation was used in rheumatoid arthritis to reduce joint pains. This cream formulation was o/w type of emulsion; hence this formulation was easily washed with plane water after application.

Formulation and Evaluation of Polyherbal Cream and Lotion for the Treatment of Psoriasis-Induced Secondary Infections.

Psoriasis is one of the most common skin diseases in humans and affects a major population worldwide. The aim of the present study is to evaluate the efficacy of selected polyherbal formulations against psoriasis-induced secondary infections. Psoriasis is one of the most common skin diseases in humans and affects a major population worldwide. The aim of the present study is to evaluate the efficacy of selected polyherbal formulations against psoriasis-induced secondary infections. Samples were collected from the scalp, behind the ears, chest and back of the patients. The microscopic examination of fungal and bacterial growth was carried out with lactophenol cotton blue stain and gram stain, respectively. Volatile constituents of essential oils were identified by GC-MS analysis, in order to investigate the relation between chemical composition and biological activity. Nutrient agar media was used for antibacterial activity whereas Sabourauds dextrose agar media was used for antifungal activity. A total of 24 isolates were obtained from 2 patients of scalp psoriasis. Anti-oxidant activity of the samples was determined using DPPH radical scavenging method. In the present study, EOs showed a good antifungal action against Malassezia pachydermatis and other microbial strains. The GC-MS analysis revealed the presence of eugenol, linalool, citral, neral, limonene, terpenes, eucalyptol and thymol in the essential oils. The formulated retention lotion 1 (L4), 2 (L5), 3 (L6) and Cream 1 (C1) showed DPPH radical scavenging activity by 23.52%, 24.48%, 28% and 5.08%, respectively. The present study concluded that most of the formulated lotions and creams showed good antimicrobial activity and may be applied topically against scalp psoriasis.

Formulation and Evaluation of a Poly Herbal Skin Care Cream containing Neem and Tulsi

Neem and Tulsi called as holy tree and herb has been used since ancient times for the treatment of various skin diseases. In this study Neem leaves Azadirachta indica and Tulsi leaves Ocimum sanctum was incorporated with various ingredients and a poly herbal cream was prepared. Neem leaves and Tulsi leaves were collected from Tirupati Local Area. Both these plants were shade dried for 4 days and size reduced using mixer grinder converted into coarse powder and passed through sieve number 22. The coarse powder was stored for further studies. Both Neem and Tulsi leaf were defatted using pet ether 60–40 and then extracted using ethanol in a soxhlet apparatus. The extraction was carried out for 3 hrs taking 100 gms of plant material with 500 ml of ethanol. The extract was evaporated in a rotary evaporator the obtained ethanol extracts were preserved for further studies in a dessicator. The extracts were incorporated with various ingredients and excipients and a poly herbal cream was formulated. The cream was evaluated for various evaluation parameters which include pH, viscosity, spreadability, centrifugation test, spectrophotometric test, accelerated stability studies and microbial stability. The accelerated stability studies were carried out for 20 days in room and elevated temperature in a stability chamber. The cream was found to be stable after centrifugation there was no phase separation after centrifugation and stability study. The microbial stability was evaluated for bacterial growth (E. coli) and yeast. The results revealed that the microbial stability of the cream incorporated no microbial growth.

Wound Healing Activity of a Traditionally Used Poly Herbal Product in a Burn Wound Model in Rats.

Background:Burns are known as one of the most common and destructive forms of injury with a vast spectrum of consequences. Despite the discovery of various antibacterial and antiseptic agents, burn wound healing still has remained a challenge to modern medicine. Plants, with a valuable traditional support, have been considered as potential agents for prevention and treatment of disorders in recent years. However, modern scientific methods should be applied to validate the claims about the therapeutic effects of the herbal products.Objectives:This study was conducted to evaluate the wound-healing activity of a poly herbal cream (PHC), retrieved from Iranian Traditional Medicine (ITM), in a rat burn wound model in Iran.Materials and Methods:In this experimental study, PHC containing aqueous extracts of Malva sylvestris and Solanum nigrum leaves and oily extract of Rosa damascena petals was used. Second-degree burn wounds were induced in four groups of five rats each. Group 1 received no treatment while groups 2, 3 and 4 were given cream base, silver sulfadiazine (SS) 1% and PHC, respectively to compare the efficacy of PHC with the negative and positive control groups. The percentage of wound healing on days 2, 6, 10 and 14 and histopathological parameters of healed wounds on the 14th day were assessed. Antioxidant and antimicrobial activities of PHC were evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and micro-dilution methods, respectively.Results:There was a significant improvement in healing percentage of PHC-treated rats in comparison to the other groups at the end of the treatment period (87.0% ± 2.1% for PHC in comparison to 32.2% ± 1.6%, 57.0% ± 5.3% and 70.8% ± 3.5% for the control, cream base and SS groups, respectively). Moreover, the healed wounds in PHC-treated animals contained less inflammatory cells and had desirable re-epithelialization with remarkable neovascularization. In addition to the antioxidant activity, PHC exhibited antibacterial effect against Staphylococcus aureus.Conclusions:Poly herbal cream experimentally and histopathologically revealed a burn wound healing activity probably due to the antioxidant, anti-inflammatory and antimicrobial activities of its phytochemical contents. Therefore, this study confirms the use of M. sylvestris, S. nigrum and R. damascena in burn prescriptions in ITM.

Clearance of Cervical Human Papillomavirus Infection by Topical Application of Curcumin and Curcumin Containing Polyherbal Cream: A Phase II Randomized Controlled Study

Curcumin and curcumin containing polyherbal preparations have demonstrated anti-microbial and anti- viral properties in pre-clinical studies. Till date no therapeutic intervention has been proved to be effective and safe in clearing established cervical human papillomavirus (HPV) infection. The present study evaluated the efficacy of Basant polyherbal vaginal cream (containing extracts of curcumin, reetha, amla and aloe vera) and of curcumin vaginal capsules to eliminate HPV infection from cervix. Women were screened by Pap smear and HPV DNA test by PCR. HPV positive women without high grade cervical neoplasias (N=287) were randomized to four intervention arms to be treated with vaginal Basant cream, vaginal placebo cream, curcumin vaginal capsules and placebo vaginal capsules respectively. All subjects were instructed to use one application of the assigned formulation daily for 30 consecutive days except during menstruation and recalled within seven days of the last application for repeat HPV test, cytology and colposcopy. HPV clearance rate in Basant arm (87.7%) was significantly higher than the combined placebo arms (73.3%). Curcumin caused higher rate of clearance (81.3%) than placebo though the difference was not statistically significant. Vaginal irritation and itching, mostly mild to moderate, was significantly higher after Basant application. No serious adverse events were noted.

A novel polyherbal microbicide with inhibitory effect on bacterial, fungal and viral genital pathogens

A polyherbal cream (Basant) has been formulated using diferuloylmethane (curcumin), purified extracts of Emblica officinalis (Amla), purified saponins from Sapindus mukorossi, Aloe vera and rose water along with pharmacopoeially approved excipients and preservatives. Basant inhibits the growth of WHO strains and clinical isolates of Neisseria gonorrhoeae, including those resistant to penicillin, tetracycline, nalidixic acid and ciprofloxacin. It has pronounced inhibitory action against Candida glabrata, Candida albicans and Candida tropicalis isolated from women with vulvovaginal candidiasis, including three isolates resistant to azole drugs and amphotericin B. Basant displayed a high virucidal action against human immunodeficiency virus HIV-1NL4.3 in CEM-GFP reporter T and P4 (Hela-CD4-LTR-βGal) cell lines with a 50% effective concentration (EC 50) of 1:20 000 dilution and nearly complete (98–99%) inhibition at 1:1000 dilution. It also prevented the entry of HIV-1(IIIB) virus into P4-CCR5 cells (EC 50 ∼1:2492). Two ingredients, Aloe and Amla, inhibited the transduction of human papillomavirus type 16 (HPV-16) pseudovirus in HeLa cells at concentrations far below those that are cytotoxic and those used in the formulation. Basant was found to be totally safe according to pre-clinical toxicology carried out on rabbit vagina after application for 7 consecutive days or twice daily for 3 weeks. Basant has the potential of regressing vulvovaginal candidiasis and preventing N. gonorrhoeae, HIV and HPV infections.

Prevention of AIDS and sexually transmitted infections (Review Paper)

Abnormal vaginal discharge due to reproductive tract infections (RTIs) is widely prevalent in the country. According to WHO, over 300 million new cases of sexually-transmitted infections (excluding HIV) occur each year. In addition to these, HIV infection is spreading rapidly in the country with over 3.7 million sero-positive cases (from zero) within 15 years.. The predominant mode of transmission of HIV is by heterosexual route. The multidrug regime for treatment is expensive (about $10,000 per year) which is beyond the reach of most of the people. No viable vaccine preventing AIDS infection is in sight. Under these circumstances, safe sex is the best recourse, which demands consistent and proper use of condoms. This does not take place to the extent necessary for preventing the transmission of HIV and other sexually-transmitted infections. A polyherbal tablet for intravaginal use by women has been developed and a polyherbal cream, for use by both men and women, which has a wide spectrum antimicrobial action. Amongst others, these inhibit the growth of Neisseria gonorrhea (including strains resistant to penicillin). normal and multidrug-resistant isolates of urinary tract E. coli, Candida albicans. Candida krusei and Candida tropicalis. Applied intravaginally, these prevent the transmission of Herpes simplex 2 and Chlamydia Irachomatis in progestin-sensitised mice. Studies conducted at the Institut Pasteur. Paris and Conrad Norfolk, USA, have demonstrated high virucidal action of these formulations against HIV . Both formulations have undergone phase I clinical trials in five major centres in India and abroad, which have indicated the complete safety of these products without any local or systemic side effects. The Drug Controller of India and the Institutional Ethics Committees have approved phase II clinical trials. The first of these trials was conducted in 88 women with abnormal vaginal discharge due to genital pathogens at the Postgraduate Institute of Medical Education and Research. Chandigarh. Every woman. who used the praneem polyherbals, once every night for seven days, experienced relief clinically. irrespective of the nature of the causative pathogen.