The multichannel motional Stark effect (MSE) diagnostic system in JT-60U has been upgraded to measure density fluctuation profile. A 16-channel fast-sampling digitizer has been added in order to measure photomultiplier-tube signals at measurement frequency of 0.5–1MHz. The new system works as a MSE and beam emission spectroscopy diagnostic. Spatially resolved electron density fluctuation profile measurement in various operation regimes is presented. In the core plasma, density fluctuation induced by rotation of tearing mode islands was observed. Temporal evolution of the fluctuation frequency agrees with that measured by Mirnov coils (poloidal and toroidal mode numbers: 2 and 1, respectively). The phases of the fluctuations on either side of the q=2 surface are inverted, which is consistent with electron cyclotron emission. These measurements show that the density fluctuation is caused by a rotating magnetic island structure induced by the tearing mode. In the scrape-off layer of a H-mode plasma with edge-localized-mode (ELM), i. e., ELMy H-mode outward propagation of strong intermittent emission corresponding to ELM crash was also observed. The propagation velocity is 0.69–2.2km∕s along the MSE measurement points, the time lag and distance between adjacent channels being 67±35μs and 70mm, respectively.