Microplastic (MP) pollution is a global issue due to its unprecedented impacts on the environment and human health. However, its place as a potential pollutant and possible impacts on plant and human health are less understood among the general public. The study was conducted to investigate the level of awareness of MP pollution and its impacts and to evaluate their practices and perceptions of plastic usage, and their awareness on regulatory measures in Sri Lanka. The convenience sampling method was employed to select the study population representative of both rural and urban areas. A questionnaire was distributed among 166 respondents belonging to the age group of 20 - 40 years. Nearly 67% and 68% of respondents were aware of MPs and their harmful impacts, respectively. Nevertheless, most respondents (66 %) were not aware of the regulatory measures introduced by the authorities to control the usage of plastic products. About 66% of respondents discarded plastic items on a daily or weekly basis indicating their widespread usage among the general public in Sri Lanka. However, a majority (85%) were unaware that burning of plastics is a prohibited action in the country. The results revealed that the awareness of MPs and their impacts were significantly linked to the gender, level of education, and occupation of respondents, though their awareness on regulatory measures did not show any relationship with the tested demographic characteristics. Unemployed respondents (98.4%) were more mindful of the non-biodegradable nature of plastics than that of students and employed respondents, indicating that the level of education has no link with their awareness of plastics and their characteristics. Despite reasonably high awareness on MPs, the study highlights the necessity of introducing effective strategies to enhance the awareness of regulatory measures to control plastic usage and safe disposal mechanisms among the general public to manage MP pollution in Sri Lanka.