The fundamental goal of the study is to examine the mediating effect of big five personality traits (agreeableness, conscientiousness, extraversion, openness and neuroticism) in the relationship between leader member exchange (LMX) and organization citizenship behavior (OCB). Population of the current research study contains employees of the universities of provincial capital of Khyber Pakhtunkhwa in Pakistan due to the rich background of this area in relation to leadership, personality traits, education lifestyle and behavior of peoples in this area. The study adopted positivist methodological approach. Purposive sampling method was used for obtaining sample for this current research (n=987). Established scale were adopted and were distributed to a larger sample size as the larger the sample the minimum error. The study documented negative significant relationship between leader member exchange and organization citizenship behavior. The relationship between leader member exchange and openness was found positive. Moreover, positive relationship was found between leader member exchange and conscientiousness, extraversion and agreeableness. The study documented negative significant relationship between leader member exchange and neuroticism. Furthermore, the relationship between organization citizenship behavior and openness was found positive, conscientiousness, extraversion and agreeableness. The study documented negative insignificant relationship between organization citizenship behavior and neuroticism. The study depicted that the relationship between organization citizenship behavior and leader member exchange is positive with openness acting as mediator. Moreover, positive relationship was found between organization citizenship behavior and leader member exchange with conscientiousness, extraversion, agreeableness and neuroticism as a mediator. 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