Güllük Gulf, located in the southeastern Aegean Sea, is significantly impacted by diverse human activities, including urban development, tourism, and intensive aquaculture. This study aims to assess the spatial distribution of trace metals in the Gulf's sediments, identify their sources, and evaluate their environmental impacts to inform effective management strategies. It also examines sediment transport patterns, which are crucial in influencing the distribution and concentration of pollutants. Establishing background concentration levels for various elements provides a baseline to assess pollution levels and identify deviations due to anthropogenic impact. Sediment samples were collected from 45 stations and analyzed for grain size, organic carbon, carbonate content, and concentrations of 26 elements. Contamination was assessed using indices such as Enrichment Factor (EF), Geoaccumulation Index (Igeo), Contamination Factor (Cf), Degree of Contamination (Cdeg), Pollution Load Index (PLI), Modified Pollution Index (MPI), and Toxic Risk Index (TRI). Statistical analyses, including Principal Component Analysis (PCA) and Cluster Analysis (CA), were used to identify pollution sources. Results indicated that Güllük Gulf's sediments are predominantly sandy, with significant silt and clay fractions in sheltered bays. Organic carbon content averaged 1.68%, with higher values near urban and aquaculture areas. Trace metal concentrations exhibited notable spatial variability, with elevated levels of Pb, Cu and Zn in specific areas, indicating both natural and anthropogenic sources. PCA identified five significant factors contributing to metal presence, linking them to lithogenic origins, aquaculture activities, and industrial inputs. TRI results showed moderate toxic risk in Asin Bay, primarily due to Ni, As and Cr. The sediment transport model of Güllük Gulf, crucial in understanding regional pollution dynamics, revealed a complex interplay of hydrodynamic forces. The study underscores the significant environmental impact of human activities on sediment quality in Güllük Gulf. Elevated trace metal levels near aquaculture sites emphasize the need for stringent environmental management practices. Effective strategies should include regular monitoring, sustainable aquaculture practices, and stakeholder engagement to mitigate environmental impacts and preserve the Gulf's ecological integrity. This comprehensive approach provides crucial insights for future research and environmental management policies aimed at addressing the complex challenges facing Güllük Gulf.