Tuberose Research Articles

Influence of Different Pulsing Solutions on Postharvest Life of Tuberose (Polianthes tuberosa L.) cv. Prajwal

Tuberose flower (Polianthes tuberosa L.) is a perennial and bulbous flowering plant, related to the Amaryllidaceae family and now it is placed in family Agavaceae. The investigation entitled “Influence of different pulsing solutions on postharvest life of tuberose (Polianthes tuberosa L.) cv. Prajwal” was carried out at the experimental farm and laboratory of Department of Floriculture and Landscape Architecture, College of Horticulture, SVPUAT, Meerut during the year of 2021-22 & 2022-23. Experiment was laid out in Completely Randomized Design with 10 treatments and 3 replications. In the present experiment we aimed at prolonging the vase life and improving the quality of cut spike of tuberose by Pulsing or hydration procedure using different noble treatments in tuberose, as the main challenge was to reduce abscission of buds, initiation of bud opening mechanism and leaf yellowing due to the sensitivity, vascular infections, high rate of respiration and inhibition of ethylene production. These differences in physiological behavior of cut stem dipped in different pulsing solution are very important to consider when developing post harvest physiology of tuberose. In the present finding we have primarily focused and observed under the light of pertinent literature available substantiated with robust evidence thus we derived the overall best treatment for different parameters for improving the vase life of tuberose that were recorded under the treatment T9 (Sucrose 15% + 8-HQC 300 ppm) followed by the treatment T6 (Sucrose 10% + 8-HQC 300 ppm) and T8 (Sucrose 15% + 8-HQC 200 ppm) resulted in most of the postharvest parameters such as amount of pulsing solution consumed (13.16, 15.82 ml), days taken to opening of floret (2.53, 2.54 days), floret diameter (3.38, 3.40 cm), vase life (10.78, 10.80 days), weight change/loss in cut spike (13.29 %, 14.52 %), fungal infection, fragrance score (2.95, 2.96), freshness index (4.97, 4.98) and moisture content (89.89, 85.60, 83.21%).

Survey on the Intensity of Peduncle Blight and Anthracnose in Tuberose (Polianthes tuberosa L.) Cultivation in Northern Karnataka

A roving survey was carried out for peduncle blight and anthracnose of tuberose caused by Lasiodiplodia theobromae and Colletotrichum gloeosporioides respectively, in major affected areas of northern Karnataka. From the results obtained, it was clear that, vegetative state is more vulnerable to the attack of the pathogens then flowering stage irrespective of the locations. Red soil type recorded higher disease severity as compared to black soil. Among the surveyed areas, the severity of peduncle blight of tuberose was maximum in Tumminakatti village (45.33%) of Haveri district while anthracnose severity was maximum in Saidapur farm (31.11%) of Dharwad district. Least disease severity of peduncle blight and anthracnose was recorded in Chandanamatti (13.33%) and Nigadi (6.67%) villages of Dharwad taluk of Dharwad district respectively.

Preharvest melatonin application mitigates arsenic-induced oxidative stress and improves vase life of tuberose (Polianthes tuberosa L.) cut flowers

Arsenic (As) stress severely hinders the growth of ornamental cut flowers. Melatonin (MLT) as a biostimulant protects plants from various abiotic stresses including As stress. However, its protective roles are not well understood in ornamental cut flowers especially tuberose. This research investigated the effects of exogenous melatonin supplementation on alleviating As stress damage in tuberose plants. In As-treated plants, growth traits and leaf chlorophyll contents markedly decreased, while application of MLT, alleviated the inhibitory effect of As on plant growth and chlorophyll content in As-treated plants. Melatonin application considerably increased the vase life of cut tuberose flowers and also improved the protein as well as proline contents in leaf tissues. Level of oxidative markers including electrolyte leakage (EL), malondialdehyde (MDA) and hydrogen peroxide (H2O2) increased in As-treated plants. Upon application of MLT, this trend was alleviated; however, the activities of enzymatic antioxidants increased when plants were subjected to As stress in combination with MLT. Results obtained in the present study suggest that exogenous MLT could alleviate negative effects of As stress on tuberose plants stimulating of oxidative defense related antioxidants. These results are beneficial to improve ornamental tuberose cut flowers production in heavy metal polluted soils or irrigation water.

Comparative Performance of Planting Materials and Standardization of Nutrient Requirement for High Yield, Quality and Early Spike Development in Tuberose (Agave amica Medik.) cv. Arka Prajwal

The study was carried out at Vegetable Research Station, TNAU, Palur, Tamil Nadu during 2021to2022. The experiment was laid out in Factorial Randomized Block Design (FRBD) with eight treatments and three replications. Planting materials used for the study were bulbs and bulblets. The eight nutrient combinations were tried with foliar application of micronutrient like zinc sulphate (0.2%), Ferrous sulphate (0.5%), Copper sulphate (0.4%) and Boric acid (0.2%) on 30, 60 and 90 DAP, individually and in combination with 25 t ha-1 FYM and recommended dose of fertilizer (200:200:200 kg ha-1 NPK). The control was maintained with RDF. Various biometric observations on growth and flowering attributes of tuberose were observed for all the treatments at different stages. The result of the present investigation revealed that among the interaction effects,B1N8[RDF + ALL 19 (NPK) + GA3@ 200 ppm (2 spray at 30 days intervals) + ZnSO4 (0.2%) + FeSO4 (0.5%) + H3BO3 (0.2 %) + CuSO4 (0.4 %)] recorded the highest plant height (95.12 cm), leaf length (51.85 cm), leaf width (2.4 cm), number of leaves per plants (42.67), rachis length (34.87cm), number of floret/spikes (46.66), flower duration (18.12 days), floret length (6.92 cm) and floret diameter (4.39cm) followed by the treatment B2N8 which recorded the highest height (90.44 cm), leaf length (48.48 cm), leaf width (2.2 cm), number of leaves per plants (39.78), rachis length (30.42 cm), number of floret/spikes (44.95), flower duration (19.93days), floret length (6.45cm) and floret diameter (4.21cm). From the interaction effect, the treatment combinationB2N1 [control (RDF) 200:200:200 NPK] recorded the lowest plant height (72.36 cm), leaf length (42.81 cm), leaf width (1.12 cm) and number of leaves per plants (43.00), rachis length (25.11cm), number of floret/spikes (37.00), flower duration (15.23days), floret length (5.04 cm) and floret diameter (3.02 cm).

Agave attenuata and Agave amica, new hosts of Tuberose mild mosaic virus in Mexico.

Agave attenuata is a Mexican wild plant originally from highlands in the central and occidental mountains of Mexico. This species, known as "swan´s neck agave", is used only as an ornamental plant in public and private gardens. No virus had previously been reported from A. attenuata before this study. In a survey conducted in a commercial greenhouse in Cuautla, Morelos, in 2018, several plants were observed with symptoms of green mosaic and streaks, consistent with a putative viral infection. Sap inoculation from symptomatic A. attenuata plants to herbaceous indicator plants (Nicotiana benthamiana and N. tabacum) failed to produce symptoms in the mechanically inoculated plants. ELISA specific test to CMV, TEV, AMV, TMV and Potyvirus Group (Agdia, Inc.), was positive only for the last one (Chen and Chang, 1998). To determine the identity of the potyvirus involved, total nucleic acid extracts from 100 mg of symptomatic leaves (Trizol reagent; Gibco BRL Life Technologies, England) were used as template in RT-PCR with genus-specific potyvirus primers POT1-POT2, which targeted the variable 5´ terminal half of the coat protein gene of potyviruses (Colinet et al. 1998). The expected 900 bp amplicon was consistently detected in 10 symptomatic A. attenuata plants whereas no PCR products were obtained from 15 asymptomatic A. attenuata plants collected from the "Agaves de México" section at the 'Botanic Garden' of the Instituto de Biología de la UNAM, México. The amplicons were sequenced by the Sanger´s method and the obtained nucleotide (nt) sequences (Acc. No KY190217.1; OP964597-598) and their derived amino acid (aa) sequences were 94.68% to 95.80% similar to an isolate of Tuberose mild mosaic virus (TuMMV; Potyvirus; (Acc. No ON116187.1) characterized from Agave amica in India (Raj et al. 2009). Interestingly, A. amica (formerly Poliantes tuberose) is also a wild Mexican plant that is geographically distributed in the central and south regions of Mexico and is currently being commercially cultivated as an ornamental plant. Plants of A. amica (n=10) showing yellow mild streak were collected from commercial greenhouse and tested positive for TuMMV by RT-PCR and Sanger sequencing (No Acc. OP964599-601 levels) described above. The derived TuMMV sequences from A. attenuata and A. amica were 99-100% similar to each other at the nt/aa level. To exclude the involvement of additional viral agents in the disease, high-throughput sequencing analysis was performed separately for each species of Agave on total RNA extracts from a composite sample of symptomatic leaf tissues using Illumina´s Next Seq 500 platform. Analysis of the obtained 13,260,700 reads (each 75 nt) by the Trinity software, with a total number of sequences of 22,793, resulted in the identification of a single viral contig of 9500 nt for A. attenuata (Acc. No OP964595). Similarly, for A. amica, 27,262,248 reads were obtained, with a total number of sequences of 23,269, resulting in the identification of a single viral contig of 8500 nt (ACC. No OP964602). These contigs showed an identity percentage of 96%/88% and 98%/96% for nucleotides and amino acids, respectively, compared to an isolate of TuMMV from India (Acc. OM293939). Mexico is a center of origin for numerous species of genus Agave which have high economic, social, and ecological impact. TuMMV could be a threat to these plants and potentially to other unknown susceptible crops. To our knowledge, this is the first report of TuMMV in A. attenuata and A. amica in Mexico. REFERENCE Chen, C. C., and Chang, C. A. 1998. Characterization of a potyvirus causing mild mosaic on tuberose. Plant Dis. 82:45-49. Colinet, D., Nguyen, M., Kummert, J., Lepoivre, P., and Xia, F. Z. 1998. Differentiation among potyviruses infecting sweet potato based on genus- and virus-specific reverse transcription polymerase chain reaction. Plant Dis. 82:223-229. Raj, S.K., Snehi, S.K., Kumar, S., Ram, T. and Goel, A.K. 2009. First report of Tuberose mild mosaic potyvirus from tuberose (Polianthes tuberosa L.) in India. Australasian Plant Dis. Notes 4, 93-95.

Genetic Variability and Heritability of Tuberose (Polianthes tuberosa) Cultivars under Agro-Climatic Conditions of Prayagraj

The present study entitled “Genetic variability and heritability of Tuberose cultivars under agroclimatic conditions of Prayagraj” was made to identify the suitable variety for growing in Prayagraj, Uttar Pradesh. Fifteen varieties were laid out in Randomized Block Design with three replications during May 2022 to February 2023 at Departmental field of Horticulture, NAI, SHUATS, Prayagraj. The observations recorded on various growth, yield and quality contributing characters. The result from the present investigation revealed the genotypes Arka Suvasini, Mexican single and Hyderabad single are identified as higher spike yield per plot which indicated that these genotypes may be shown for higher yield at Prayagraj Agro-climatic condition. The PCV were higher than GCV for all the characters taken in to consideration. High magnitude of GCV and PCV was recorded for No. of bulblets per plant, weight of bulb per plant , yield of bulb per hectare and weight of bulb and least estimates for PCV and GCV was observed for no. of leaves at 6 month after planting. Heritability and genetic advance indicate that the additive nature of gene action and reliability of those characters for selection and emerged as ideal traits for improvement through selection.

Effect of Varying Levels of pH and Edible Dyes on Tinting and Vase Life of Tuberose

Aims: The objective of this study is to explore how manipulating pH levels and incorporating edible dyes can extend the vase life, enhance the tinting, and improve the overall quality of tuberose flowers (Polianthes tuberosa).
 Study Design: The experiment was designed as a complete randomized design with three replications and a total of twenty-eight treatments.
 Place and Duration of Study: The experiment was conducted at the Horticulture lab in Department of Horticulture, School of Agriculture, Lovely Professional University, Phagwara, Punjab. The study was carried out over a specific duration in 2022.
 Methodology: Tuberose (Polianthes tuberosa) flowers were obtained from a local supplier. The flowers were carefully selected based on their uniformity in size and maturity. The study followed a completely randomized design (CRD) with 28 combinations of treatments. The pH levels included three treatments (pH 4, pH 5, and pH 6), while the edible dyes consisted of three treatments (Apple green, Lemon yellow, and orange red) at varying concentrations (1%, 2.5%, and 4%).
 Results: This research reveals that the best conditions for attaining the desired tinting effect on tuberose flowers are a slightly acidic pH level (pH 5) and a moderate concentration of lemon-yellow color (2.5%). This treatment combination was suitable for increasing the water uptake, floret opening, percent increase in floret opening and vase life. While the concentration of the dye influences the strength of the coloration, the pH level has a significant impact on how the color solution is taken up and absorbed by the flowers.
 Conclusion: The results of this study demonstrate that maintaining a pH level of 5 and using a moderate concentration (2.5%) of lemon-yellow color positively impacted both the tinting process and the longevity of cut tuberose flowers in vases.

Effects of different preservative solutions on storage properties of tuberose (Agave amica L.) cut flowers

Effects of different preservative solutions on storage properties of tuberose (Agave amica L.) cut flowers

Nutrients Content of Polianthes tuberosa Plant Waste Fermentation with Aspergillus niger as Alternative Feed Ingredients for Ruminant

The purpose of this study was to analyze the nutritional content such as organic matter, crude protein, crude fat, crude fiber, and non-nitrogen extract materials from the waste of the tuberose flower (Polianthes tuberosa) that was fermented by Aspergillus niger as ruminant feed. The materials used were Aspergillus niger with the availability of inoculant 2.46 x 108 CFU/g, tuberose flower waste, water and molasses. The research method uses the experimental method consisting of 5 treatments and 4 replications. Each treatment used a different dose of Aspergillus niger inoculant, namely (P0) control without fermentation, (P1) 1 x 107 CFU/g dry matter (DM) material, (P2) 3 x 107 CFU/g (DM) material, (P3) 6 x 107 CFU/g (DM) material and (P4) 9 x 107 CFU/g (DM) material, each treatment required 200 grams of tuberose flower waste.very significant (P<0.01) effect onnutritional content Polianthes tuberosa mfermented nature. The conclusion of the study was that the administration of Aspergillus niger at a dose of 9 x 107 CFU/g (DM) material was the best dose for Polianthes tuberosa fermentation with a content of 83,87±0,69 % organic matter, 11,50±0,01% crude protein, 0,31±0,08% CF, 18,62±0,23% crude fiber dan 69,57±0,18% non-nitrogen extract materials.

Response of Foliar Application of GA3 and Paclobutrazol on Flowering and Vase Life of Tuberose (Polianthes tuberosa L.)

The present investigation “Response of foliar application of GA3 and paclobutrazol on flowering and vase life of Tuberose (Polianthes tuberosa L.)” was carried out was carried out in Horticulture Research Farm at the Department of Floriculture and Landscape Architecture, IGKV, Raipur, Chhattisgarh, India, 2017-18 and 2018-19. The experiment was laid out in Factorial Randomized Block Design with three replication comprising treatment combination of two PGRs (GA3 and Paclobutrazol) and two tuberose cultivars (Prajwal and Bidhan Ujjwal). Two PGR’s namely Gibberellins (GA3) and Paclobutrazol (PBZ) were taken as growth promoter and growth retardant, respectively. In this contest, an experiment was conducted to investigate the effect of different levels of growth promoter and retardant on growth, flowering and vase life of different cultivars of Tuberose. The result indicated that the vegetative growth, flowering and vase life were significantly influenced by different plant growth regulators and cultivars. The maximum plant height was recorded with cv. Prajwal (V1) as compared to cv. Bidhan Ujjwal (V2). While, minimum number days to first spike emergence (117.33 days), days to first flower opening (131.83 days) was recorded with cv. Bidhan Ujjwal. Among the growth regulators treatments GA3 150 ppm (30 and 50 DAP). However, maximum length of spike (94.72 cm), length of rachis (30.35 cm), length of florets (5.8 cm), diameter of florets (4.32 cm), Number of florets spike-1 (43.41) and Vase life (10.17 days) was recorded with cv. Prajwal. Among the growth regulators treatments GA3 150 ppm (30 and 50 DAP).

Influence of Vermicompost and Bio-fertilizers on Growth and Flowering of Tuberose (Polianthes tuberosa L.) cv. Prajwal

The experiment was planned to sustainably improve the growth and yield of tuberose using bio-fertilizers and organic manures. performed in pots in completely randomized design (CRD) with 11 treatments having four replications to study the response of organic manures, chemical fertilizers and bio fertilizers on growth and flower yield of tuberose (Polianthes tuberosa L.) cv. Prajwal. The treatment containing sand + vermicompost (4:1) + Bio-fertilizers (Azotobacter&Pseudomonas sp.) was significantly superior in terms of plant height (70.50 cm), length of leaf (36.98 cm), Number of leaf (25.67), spike length (66.58 cm), rachis length (27.23 cm), diameter of stem (1.096 cm), florets number per spike (35), number of opened florets per spike (33.67), spikes number per clump, spike weight (75.33 g), bulb number per clump (21), bulb diameter (1.20 cm), bulb weight (3.98 g). While lowest values of these parameters were observed in the control (sand). Minimum days taken to bulb sprouting (21.75 days), days taken to complete bulb sprouting (24.50 days), days taken to spike emergence (93 days) and days taken to opening of first floret (106.75 days) were observed in sand + inorganic manures. Treatment having Sand + vermicompost (4:1) + Bio-fertilizers (Azotobacter&Pseudomonas sp. was observed best to promote growth and flowering of tuberose.

Biochar Combined with Foliar Application of Chelated and Nano Iron Enhanced Qualitative and Quantitative Attributes of Polianthes tuberosa, L. Plants

Biochar Combined with Foliar Application of Chelated and Nano Iron Enhanced Qualitative and Quantitative Attributes of Polianthes tuberosa, L. Plants

Polianthes tuberosa-Mediated Silver Nanoparticles from Flower Extract and Assessment of Their Antibacterial and Anticancer Potential: An In Vitro Approach.

Plant-mediated metallic nanoparticles have beenreported for a diversified range of applications in biological sciences. In the present study, we propose the Polianthes tuberosa flower as a reducing and stabilizing agent for the synthesis of silver nanoparticles (PTAgNPs). The PTAgNPs were exclusively characterized using UV-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), atomic force microscopy, zeta potential, and transmission electron microscopy (TEM) studies. In a biological assay, we investigated the antibacterial and anticancer activity of silver nanoparticles in the A431 cell line. The PTAgNPs demonstrated a dose-dependent activity in E. coli and S. aureus, suggesting the bactericidal nature of AgNPs. The PTAgNPs exhibited dose-dependent toxicity in the A431 cell line, with an IC50 of 54.56 µg/mL arresting cell growth at the S phase, as revealed by flow cytometry analysis. The COMET assay revealed 39.9% and 18.15 severities of DNA damage and tail length in the treated cell line, respectively. Fluorescence staining studies indicate that PTAgNPs cause reactive oxygen species (ROS) and trigger apoptosis. This research demonstrates that synthesized silver nanoparticles have a significant effect on inhibiting the growth of melanoma cells and other forms of skin cancer. The results show that these particles can cause apoptosis or cell death in malignant tumor cells. This suggests that they could be used to treat skin cancers without harming normal tissues.

Isolation and Evaluation of Perfume from Flower of Tuberose (Polianthes Tuberosa)

Isolation and Evaluation of Perfume from Flower of Tuberose (Polianthes Tuberosa)

Comparison of dyes and preservative solutions on the vase life of Polianthes tuberosa L.

Comparison of dyes and preservative solutions on the vase life of Polianthes tuberosa L.

Effect of Nitrogen and Phosphorus on Flowering, Bulb Yield and Nutrient Contents in Leaves of Tuberose (Polianthes Tuberose L.) cv. Prajwal

Effect of Nitrogen and Phosphorus on Flowering, Bulb Yield and Nutrient Contents in Leaves of Tuberose (Polianthes Tuberose L.) cv. Prajwal

Effect of Fertilization and Planting Date on the Production and Shelf Life of Tuberose

The tuberose, Agave amica, is an ornamental plant appreciated for its oils. The objective of this study was to evaluate the effect of planting dates (April, May and June), dose of NPK (N80-P60-K40, N300-P200-K200, N100-P50-K50 and N00-P00-K00), and fertilizer sources (chemical, organic, combined and control) on tuberose production, flower quality and postharvest shelf life. The physiological variables spike characteristics, leaf color, biomass, and postharvest flower quality were evaluated. The results show that the best planting date is in June; plants planted in June flowered earlier (156 days) and had better flower quality. The fertilization formula N300-P200-K200 produced a higher number of spikes (1.32) and flowers (38.93), a larger stem diameter (0.9 cm), and promoted fewer days to flowering (188d). Plants fertilized with chemical fertilizer had fewer yellow leaves, a larger number of spikes (1.41), a longer spike length (26.89 cm), and a higher number of flowers/spikes (39.28), corms/plants (31.03), and open flowers on the ninth day in vase (13.14) and heavier stems with spikes (134.80 g). In conclusion, the dose of N300-P200-K200 from chemical source and planting in June produced the best flower quality and the shortest production cycle.

Effect of Bulb Size and Climatic Conditions on Vegetative and Reproductive Attributes of Polianthes tuberosa L. cultivated in Algeria

Tuberose (Polianthes tuberosa L.) is an ornamental and medicinal plant, cultivated in tropical and subtropical regions for its fragrant cut flowers and perfume industries, P. tuberosa flowers are the source of the highest-quality tuberose oil that remains today the most expensive raw material in perfumery. Furthermore, bulb size influences the flowering of P. tuberosa L. and creates a problem for tuberose flower production. Aims in this study were climatic condition and bulbs size variation, which were investigated to enhance the bulb size and climatic condition for improving tuberose production in Algeria. A culture of P. tuberosa L. bulbs was conducted in the field and under shelter using different bulb diameters, growing and flowering parameters were recorded for each plant, from planting to maturity. According to the obtained results, a good growth of tuberose was noticed with the big bulbs (diameter≥2 cm), i.e., bulb germination, leaf emergence, and the number of leaves by dominance for the bulbs cultivated under shelter, compared to those grown in the field. Regarding flowering, it was influenced mainly by bulb size, where only big bulbs gave flowers, and flowering characteristics were improved by the under shelter culture neither in the field. In general and according to the present work, for a good growth and for a better production of P. tuberosa flowers, bulbs culture under shelter using bulbs of 2-4 cm in diameter were recommended.

In planta transformation of Polianthes tuberosa for concomitant knockdown of flp-1, flp-12 and flp-18 genes induced root-knot nematode resistance

Polianthes tuberosa is a lucrative crop owing to its huge demand in floriculture, perfume and pharmaceutical industry. Root-knot nematodes, especially Meloidogyne incognita poses a major impediment on tuberose yield. Currently nematode management relies on the use of hazardous nematicides and other inefficacious means viz. crop rotation and soil solarization. The ongoing search for an ecologically sustainable and target specific approach, led to the progression of genetic modification based techniques to target genes critical for nematode survival. FMRFamide like peptide (FLPs) genes have drawn interest as prospective targets because of their diverse physiological roles in nematodes. Here, the effect of concomitant silencing of three vital nematode FLP genes (Mi-flp-1, Mi-flp-12 and Mi-flp-18) using host induced RNAi in tuberose was assessed against M. incognita. Firstly, the fusion (flp-1, flp-12 and flp-18) gene cassette was functionally validated using host-delivered RNAi in tobacco model system. Transgenic tobacco expressing dsRNA of fusion gene significantly reduced the nematode multiplication factor (MF) by 82.6%. Since, genetic transformation of tuberose has been neglected so far, and transgenic development is an inevitable step for host-generated RNAi, an Agrobacterium mediated transformation protocol was optimized in tuberose. Tuberose tubers were pricked at the apical meristem of the axis and at the tiny white meristematic buds emerging from them, followed by infection with Agrobacterium hosting pCAMBIA 3301 vector. Maximum transformation efficiency was achieved with 200 μM acetosyringone as observed from the GUS staining. For HIGS, fusion transgenic events were developed in tuberose and molecular analysis confirmed the presence and integration of fusion transgene using PCR, qRT-PCR and Southern blot analysis. Bioefficacy analysis of transgenic plants against M. incognita resulted in 73.75% reduction in nematode fecundity, thereby demonstrating the effectiveness of the three flp genes for nematode management in tuberose. This study established first ever report of in planta Agrobacterium-mediated transformation of tuberose for host delivered RNAi for M. incognita resistance.

Efficacy of seaweed and neemcake on growth and flowering attributes of tuberose (Polianthes tuberosa L.) in the plains of West Bengal

An experiment was carried to evaluate the effect of combined application of seaweed and neem cake on growth and flowering of tuberose (Polianthes tuberosa)cv. Prajwal. The study was laid out under Randomized Block Design (RBD) with eleven treatments and three replications where different combinations of neem cake and seaweed were compared with control. The result of the experiment revealed that the application of neem cake and seaweed significantly affected the quantitative and qualitative characters of tuberose. Among all the treatments, treatment T11 (Seaweed 4g/m2 + Neem cake 0.4 kg/m2 ) was found most effective regarding the growth and flowering attributes in comparison to control. The treatment T11 (Seaweed 4g/m2 + Neem cake 0.4 kg/m2 ) also gave most promising result regarding vase life and spike yield.