Polarization Shift Research Articles

The early excitatory action of striatal cholinergic-GABAergic microcircuits conditions the subsequent GABA inhibitory shift

Cholinergic interneurons of the striatum play a role in action selection and associative learning by activating local GABAergic inhibitory microcircuits. We investigated whether cholinergic-GABAergic microcircuits function differently and fulfill a different role during early postnatal development, when GABAA actions are not inhibitory and mice pups do not walk. We focused our study mainly on dual cholinergic/GABAergic interneurons (CGINs). We report that morphological and intrinsic electrophysiological properties of CGINs rapidly develop during the first post-natal week. At this stage, CGINs are excited by the activation of GABAA receptors or GABAergic synaptic inputs, respond to cortical stimulation by a long excitation and are linked by polysynaptic excitations. All these excitations are replaced by inhibitions at P12-P15. Early chronic treatment with the NKCC1 antagonist bumetanide to evoke premature GABAergic inhibitions from P4 to P8, prevented the GABA polarity shift and corticostriatal pause response at control postnatal days. We propose that early excitatory cholinergic-GABAergic microcircuits are instrumental in the maturation of GABAergic inhibition.

SARS-CoV-2 niches in human placenta revealed by spatial transcriptomics

Functional placental niches are presumed to spatially separate maternal-fetal antigens and restrict the vertical transmission of pathogens. We hypothesized a high-resolution map of placental transcription could provide direct evidence for niche microenvironments with unique functions and transcription profiles. We utilized Visium Spatial Transcriptomics paired with H&E staining to generate 17,927 spatial transcriptomes. By integrating these spatial transcriptomes with 273,944 placental single-cell and single-nuclei transcriptomes, we generated an atlas composed of at least 22 subpopulations in the maternal decidua, fetal chorionic villi, and chorioamniotic membranes. Comparisons of placentae from uninfected healthy controls (n= 4) with COVID-19 asymptomatic (n= 4) and symptomatic (n= 5) infected participants demonstrated that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in syncytiotrophoblasts occurred in both the presence and the absence of maternal clinical disease. With spatial transcriptomics, we found that the limit of detection for SARS-CoV-2 was 1/7,000 cells, and placental niches without detectable viral transcripts were unperturbed. In contrast, niches with high SARS-CoV-2 transcript levels were associated with significant upregulation in pro-inflammatory cytokines and interferon-stimulated genes, altered metallopeptidase signaling (TIMP1), with coordinated shifts in macrophage polarization, histiocytic intervillositis, and perivillous fibrin deposition. Fetal sex differences in gene expression responses to SARS-CoV-2 were limited, with confirmed mapping limited to the maternal decidua in males. High-resolution placental transcriptomics with spatial resolution revealed dynamic responses to SARS-CoV-2 in coordinate microenvironments in the absence and presence of clinically evident disease. This work was supported by the NIH (R01HD091731 and T32-HD098069), NSF (2208903), the Burroughs Welcome Fund and the March of Dimes Preterm Birth Research Initiatives, and a Career Development Award from the American Society of Gene and Cell Therapy.

Soliton interactions and Yang–Baxter maps for the complex coupled short‐pulse equation

AbstractThe complex coupled short‐pulse equation (ccSPE) describes the propagation of ultrashort optical pulses in nonlinear birefringent fibers. The system admits a variety of vector soliton solutions: fundamental solitons, fundamental breathers, composite breathers (generic or nongeneric), as well as so‐called self‐symmetric composite solitons. In this work, we use the dressing method and the Darboux matrices corresponding to the various types of solitons to investigate soliton interactions in the focusing ccSPE. The study combines refactorization problems on generators of certain rational loop groups, and long‐time asymptotics of these generators, as well as the main refactorization theorem for the dressing factors that leads to the Yang–Baxter property for the refactorization map and the vector soliton interactions. Among the results obtained in this paper, we derive explicit formulas for the polarization shift of fundamental solitons that are the analog of the well‐known formulas for the interaction of vector solitons in the Manakov system. Our study also reveals that upon interacting with a fundamental breather, a fundamental soliton becomes a fundamental breather and, conversely, that the interaction of two fundamental breathers generically yields two fundamental breathers with a polarization shifts, but may also result into a fundamental soliton and a fundamental breather. Explicit formulas for the coefficients that characterize the fundamental breathers, as well as for their polarization vectors are obtained. The interactions of other types of solitons are also derived and discussed in detail and illustrated with plots. New Yang–Baxter maps are obtained in the process.

Pharmacological inhibition of DNMT1 restores macrophage autophagy and M2 polarization in Western diet–induced nonalcoholic fatty liver disease

Nonalcoholic fatty liver disease (NAFLD) is associated with an increased ratio of classically activated M1 macrophages/Kupffer cells to alternatively activated M2 macrophages, which plays an imperative role in the development and progression of NAFLD. However, little is known about the precise mechanism behind macrophage polarization shift. Here, we provide evidence regarding the relationship between the polarization shift in Kupffer cells and autophagy resulting from lipid exposure. High-fat and high-fructose diet supplementation for 10weeks significantly increased the abundance of Kupffer cells with an M1-predominant phenotype in mice. Interestingly, at the molecular level, we also observed a concomitant increase in expression of DNA methyltransferases DNMT1 and reduced autophagy in the NAFLD mice. We also observed hypermethylation at the promotor regions of autophagy genes (LC3B, ATG-5, and ATG-7). Furthermore, the pharmacological inhibition of DNMT1 by using DNA hypomethylating agents (azacitidine and zebularine) restored Kupffer cell autophagy, M1/M2 polarization, and therefore prevented the progression of NAFLD. We report the presence of a link between epigenetic regulation of autophagy gene and macrophage polarization switch. We provide the evidence that epigenetic modulators restore the lipid-induced imbalance in macrophage polarization, therefore preventing the development and progression of NAFLD.

The Impact of Hysteresis Effect on Device Characteristic and Reliability for Various Fin-Widths Tri-Gate Hf0.5Zr0.5O2 Ferroelectric FinFET

In this study, we developed a facilitated ferroelectric high-k/metal-gate n-type FinFET based on Hf0.5Zr0.5O2. We investigated the impact of the hysteresis effect on device characteristics of various fin-widths and the degradation induced by stress on the ferroelectric FinFET (Fe-FinFET). We clarified the electrical characteristics of the device and conducted related reliability inspections. For the Fe-FinFET, the hysteresis behavior of the Hf0.5Zr0.5O2-based gate stack in the Si-fin body is apparent, especially at narrower fin-widths, which affects device performance and reliability under voltage stress. The gate ferroelectric film is worsened after voltage stress with higher impact ionization, resulting in hysteresis degradation and serious induced device performance degradation. It is suggested that the hysteresis degradation is caused by both a shift in polarization of the gate ferroelectric film and generation of interface traps after high-energy carrier stress, which was confirmed by crystal structure inspection.

Effect of Wear-Corrosion of Reduced Graphene Oxide Functionalized with Hyaluronic Acid on Inflammatory and Proteomic Response of J774A.1 Macrophages

The presence of a worn surface in the implanted material, as in the case of a replacement of a damaged osteoarticular joint, is the normal condition after implantation. This manuscript focuses precisely on the comparative study of the cellular behavior on worn CoCr surfaces, analyzing the effect of different surface modifications on macrophages’ responses. CoCr surfaces were modified by the deposition of electrochemically reduced graphene oxide (CoCrErGO), followed by additional surface functionalization with hyaluronic acid (CoCrErGOHA). After the wear corrosion processes, the macrophage response was studied. In addition, macrophage supernatants exposed to the surfaces, before and after wear, were also evaluated for osteoblast response through the analysis of the metabolic activity, plasma membrane damage, and phosphatase alkaline activity (ALP). The proteomic analysis and the quantitative TNF-α/IL-10 ratios of the J774A.1 macrophages exposed to the surfaces under study showed a polarization shift from M0 (basal state) to M1, associated with the pro-inflammatory response of all surfaces. A lower M1 polarization was observed upon exposure to the surface modification with ErGO, whereas posterior HA functionalization attenuated, even more, the M1 polarization. The wear corrosion process contributed to inflammation and exacerbated the M1 polarization response on macrophages to CoCr, which was diminished for the ErGO and attenuated the most for the ErGOHA surfaces. Comparative proteomics showed that the pathways related to M1 polarization were downregulated on the surfaces of CoCrErGOHA, which suggests mechanisms for the observed attenuation of M1 polarization. The suitable immuno-modulatory potential induced by the ErGOHA surface, with and without wear, together with the stimulation of ALP activity in osteoblasts induced by macrophage supernatants, promotes the mineralization processes necessary for bone repair. This makes it feasible to consider the adsorption of ErGOHA on CoCr as a recommended surface treatment for the use of biomaterials in osseous joint applications.

Challenging the antiquity of the Cradle of Humankind, South Africa: Geochronological evidence restricts the age of Eurotomys bolti and Parapapio to less than 2.3 Ma at Waypoint 160, Bolt's Farm

Waypoint 160 is a paleocave at Bolt's Farm in the ‘Cradle of Humankind,’ South Africa. It is known for the novel murid taxa Eurotomys bolti, argued to be morphologically intermediate between Eurotomys pelomyoides from Langebaanweg (∼5.1 Ma) and the earliest Otomyinae from Makapansgat Limeworks (∼3.0–2.6 Ma). Based on the presence of this specimen, an age of ∼4.5 Ma was inferred for Waypoint 160, making it far older than other Cradle sites. This biochronological age was used to argue that Parapapio and Cercopithecoides fossils from Waypoint 160 were the oldest in the region. Here, we provide a detailed sedimentological context for the in-situ deposits at Waypoint 160. We have identified interior cave deposits, in contrast to other sites at Bolt's Farm. Petrography confirms that one unit (facies D) contains in-situ microfaunal fossils, indicating the likely provenience of the E. bolti specimen. Palaeomagnetic analysis shows four periods of magnetic polarity in the sequence. Using U–Pb ages as chronological pins, we argue that the upper part of the sequence records a polarity change at the end of the Olduvai subChron (1.78 Ma). The lower part of the sequence records a polarity shift from normal to reversed that likely relates to the Feni subChron (2.16–2.12 Ma), based on a basal flowstone U–Pb age of 2.269 ± 0.075 Ma. Together this points to a depositional window of ∼500 ka, with the Parapapio and E. bolti tentatively attributed to the micromammal fossil-bearing layers dating to ∼2.27–2.07 Ma. This has significant implications for other biochronological dates in South Africa, as E. bolti is now less than ∼2.27 Ma, younger than the oldest Otomyinae at Makapansgat Limeworks and thus not ancestral to them. This chronology for Waypoint 160 challenges the presence of older, early to mid-Pliocene deposits >3.20 Ma in the Gauteng portion of the Cradle.

Research on Coordinated Control Strategy of Energy Storage Type Railway Power Conditioner

In order to improve the power quality of high-speed railway traction power supply system and enhance the robust stability of railway power conditioner (RPC), a coordinated control strategy based on the energy storage system (ESS) integrated RPC (ESS-RPC) is proposed in this article. It is found that the dc impedance characteristics of the back-to-back system are different when the parameters of the ESS-RPC system are perturbed under conventional control, which will affect the dc voltage of the back-to-back system and then affect the power quality of the traction system. In this article, it is analyzed that the stability difference of the bidirectional power flow of ESS-RPC under parameter perturbation is due to the negative impedance of the dc terminal and the right shift of the dominant pole. After the analysis, a coordinated control strategy of ESS-RPC is proposed to solve this problem. The small-signal analysis of the ESS-RPC system under conventional control and coordinated control is carried out, respectively, and the robust stability of the two control strategies is judged by the Nyquist stability criterion. Simulation and experimental verification are carried out in the MATLAB/Simulink environment and semisimulation platform (Modeling Tech) to analyze the impact of the proposed coordinated control strategy on the power quality of the traction power supply system and verify the effectiveness and accuracy of this coordinated control strategy.

Toll-Like Receptor Signaling in the Pathogenesis of Chronic Dacryocystitis: Implication of c-FOS Transcription Factor and its Downstream Effector Chemokine Genes CCL2, CCL4, CXCL3, CXCR4 with a Shift of the M1/M2 Macrophage Phenotype.

TLRs are fundamental elements in the orchestration of the innate immune system. These receptors seem to be responsible for the inflammation and fibrosis in chronic dacryocystitis. The aim of the present study was to investigate the role of the toll-Like receptors (TLR2 and TLR4) signaling pathway and its downstream effector chemokine genes in the pathogenesis of chronic dacryocystitis. This study was conducted on 20 patients diagnosed with chronic dacryocystitis and underwent external dacryocystorhinostomy. Estimation of gene expression of TLR2, TLR4, CCL2, CCL4, CXCL3, CXCR4, and c-FOS genes in the lacrimal sac tissues was performed together with the assessment of the inflammatory markers TNFα, IL-1β, IFN-γ, and IL-22. Histopathological examination of the lacrimal sac walls using hematoxylin and eosin (H&E) stain, in addition to immunohistochemical staining of the CD68 and CD163 macrophage markers, was also performed. Our results showed that TLR2, TLR4, and c-FOS gene expressions were significantly increased in the chronic dacryocystitis group with a subsequent increase in their downstream effector chemokine genes CCL2, CCL4, and CXCL3. This up-regulation of genes was accompanied by macrophage shift of polarization toward the M1 pro-inflammatory phenotype (increased CD68 and decreased CD163 expression), leading to increased levels of the pro-inflammatory cytokines (TNF- α, IL-1β and IFN-γ) and decreased anti-inflammatory marker IL-22 with chronic dacryocystitis. It is essential to fine-tune TLR activation through emerging therapeutic approaches. Targeting TLR signaling at the level of receptors or downstream adaptor molecules represents a new challenge for treating chronic dacryocystitis.

Multilevel polarization shift keying modulation for turbulence-robust underwater optical wireless communication.

Turbulence is an intractable issue for underwater optical wireless communication (UOWC). Most literature has been mainly focused on the modeling of turbulence channels and performance analysis rather than mitigation of the turbulence effect, especially from the experimental aspects. In this paper, a multilevel polarization shift keying (PolSK) modulation based UOWC system is established utilizing a 15 m-long water tank, and the system performance is investigated under specific temperature gradient-induced turbulence and various transmitted optical powers. Experimental results show the feasibility of the PolSK in alleviating the effect of turbulence, and the bit error rate performance significantly outperforms traditional intensity-based modulation schemes which have difficulty in obtaining an optimal decision threshold in a turbulence channel.

All-Atom Molecular Dynamics Simulations Indicated the Involvement of a Conserved Polar Signaling Channel in the Activation Mechanism of the Type I Cannabinoid Receptor.

The type I cannabinoid G protein-coupled receptor (CB1, GPCR) is an intensely investigated pharmacological target, owing to its involvement in numerous physiological functions as well as pathological processes such as cancers, neurodegenerative diseases, metabolic disorders and neuropathic pain. In order to develop modern medications that exert their effects through binding to the CB1 receptor, it is essential to understand the structural mechanism of activation of this protein. The pool of atomic resolution experimental structures of GPCRs has been expanding rapidly in the past decade, providing invaluable information about the function of these receptors. According to the current state of the art, the activity of GPCRs involves structurally distinct, dynamically interconverting functional states and the activation is controlled by a cascade of interconnecting conformational switches in the transmembrane domain. A current challenge is to uncover how different functional states are activated and what specific ligand properties are responsible for the selectivity towards those different functional states. Our recent studies of the μ-opioid and β2-adrenergic receptors (MOP and β2AR, respectively) revealed that the orthosteric binding pockets and the intracellular surfaces of these receptors are connected through a channel of highly conserved polar amino acids whose dynamic motions are in high correlation in the agonist- and G protein-bound active states. This and independent literature data led us to hypothesize that, in addition to consecutive conformational transitions, a shift of macroscopic polarization takes place in the transmembrane domain, which is furnished by the rearrangement of polar species through their concerted movements. Here, we examined the CB1 receptor signaling complexes utilizing microsecond scale, all-atom molecular dynamics (MD) simulations in order to see if our previous assumptions could be applied to the CB1 receptor too. Apart from the identification of the previously proposed general features of the activation mechanism, several specific properties of the CB1 have been indicated that could possibly be associated with the signaling profile of this receptor.

Key Role of Mesenchymal Stromal Cell Interaction with Macrophages in Promoting Repair of Lung Injury.

Lung macrophages (Mφs) are essential for pulmonary innate immunity and host defense due to their dynamic polarization and phenotype shifts. Mesenchymal stromal cells (MSCs) have secretory, immunomodulatory, and tissue-reparative properties and have shown promise in acute and chronic inflammatory lung diseases and in COVID-19. Many beneficial effects of MSCs are mediated through their interaction with resident alveolar and pulmonary interstitial Mφs. Bidirectional MSC-Mφ communication is achieved through direct contact, soluble factor secretion/activation, and organelle transfer. The lung microenvironment facilitates MSC secretion of factors that result in Mφ polarization towards an immunosuppressive M2-like phenotype for the restoration of tissue homeostasis. M2-like Mφ in turn can affect the MSC immune regulatory function in MSC engraftment and tissue reparatory effects. This review article highlights the mechanisms of crosstalk between MSCs and Mφs and the potential role of their interaction in lung repair in inflammatory lung diseases.

Identifying Splitting Through Sentiment Analysis.

In Kernerg's Object Relations Theory model of personality pathology, splitting, the mutual polarization of aspects of experience, is thought to result in a failure of identity integration. The authors sought to identify a clinician-independent, automated measure of splitting by examining 54 subjects' natural speech. Splitting in these individuals, recruited from the community, was investigated and evaluated with a shortened version of the Structured Interview of Personality Organization (STIPO-R). A type of automated sentiment textual analysis called VADER was applied to transcripts from the section of the STIPO-R that probes identity integration. Higher variability in speech valence, more negative minimum valence, and more frequent shifts in valence polarity were associated with more severe identity disturbance. The authors concluded that the degree of splitting elicited during the description of self and others is related to the degree of identity disturbance, and to the degree of negativity and instability of these descriptions of self and others.

Product review opinion based on sentiment analysis

Sentiment analysis is a natural language processing (NLP) technique for determining emotional tone in a body of text. Using product reviews in sentiment analysis and opinion mining various methods have been developed previously. Although, existing product review analyzing techniques could not accurately detect the product aspect and non-aspect. Hence a novel Detach Frequency Assort is proposed to detect the product aspect term using TF-ISF (Term frequency-inverse sentence frequency) with Part of Speech (POS) tags for sentence segmentation and additionally using Feedback Neural Network to combine product aspect feedback loop. Furthermore, decision-making problem occurs during classification of sentiments. Hence, to solve this problem a novel technique named, Systemize Polarity Shift is proposed in which flow search based Support Vector Machine (SVM) with Bag of Words model classifies pre-trained review comments as positive, negative, and neutral sentiments. Moreover, the identification of specific products is not focused in sentiment analysis. Hence, a novel Revival Extraction is proposed in which a specific product is extracted based on thematic analysis method to obtain accurate data. Thus, the proposed Product Review Opinion framework gives effective optimized results in sentiment analysis with high accuracy, specificity, recall, sensitivity, F1-Score, and precision.

Real-Time Ellipsometric Surface Plasmon Resonance Sensor Using Polarization Camera May Provide the Ultimate Detection Limit.

Ellipsometric Surface Plasmon Resonance (SPR) sensors are known for their relatively simple optical configuration compared to interferometric and optical heterodyne phase interrogation techniques. However, most of the previously explored ellipsometric SPR sensors based on intensity measurements are limited by their real-time applications because phase or polarization shifts are conducted serially. Here we present an ellipsometric SPR sensor based on a Kretschmann-Raether (KR) diverging beam configuration and a pixelated microgrid polarization camera. The proposed methodology has the advantage of real-time and higher precision sensing applications. The short-term stability of the measurement using the ellipsometric parameters tanψ and cos(Δ) is found to be superior over direct SPR or intensity measurements, particularly with fluctuating sources such as laser diodes. Refractive index and dynamic change measurements in real-time are presented together with Bovine Serum Albumin (BSA)-anti-BSA antibody binding to demonstrate the potential of the developed sensor for biological sensing applications with a resolution of sub-nM and down to pM with additional optimization. The analysis shows that this approach may provide the ultimate detection limit for SPR sensors.

Storm time polar cap expansion: interplanetary magnetic field clock angle dependence

Abstract. It is well known that the polar cap, delineated by the open–closed field line boundary (OCB), responds to changes in the interplanetary magnetic field (IMF). In general, the boundary moves equatorward when the IMF turns southward and contracts poleward when the IMF turns northward. However, observations of the OCB are spotty and limited in local time, making more detailed studies of its IMF dependence difficult. Here, we simulate five solar storm periods with the coupled model consisting of the Open Geospace General Circulation Model (OpenGGCM) coupled with the Coupled Thermosphere Ionosphere Model (CTIM) and the Rice Convection Model (RCM), i.e., the OpenGGCM-CTIM-RCM, to estimate the location and dynamics of the OCB. For these events, polar cap boundary location observations are also obtained from Defense Meteorological Satellite Program (DMSP) precipitation spectrograms and compared with the model output. There is a large scatter in the DMSP observations and in the model output. Although the model does not predict the OCB with high fidelity for every observation, it does reproduce the general trend as a function of IMF clock angle. On average, the model overestimates the latitude of the open–closed field line boundary by 1.61∘. Additional analysis of the simulated polar cap boundary dynamics across all local times shows that the MLT of the largest polar cap expansion closely correlates with the IMF clock angle, that the strongest correlation occurs when the IMF is southward, that during strong southward IMF the polar cap shifts sunward, and that the polar cap rapidly contracts at all local times when the IMF turns northward.

Characterizing T cell responses to enzymatically modified beta cell neo-epitopes.

Previous studies verify the formation of enzymatically post-translationally modified (PTM) self-peptides and their preferred recognition by T cells in subjects with type 1 diabetes (T1D). However, questions remain about the relative prevalence of T cells that recognize PTM self-peptides derived from different antigens, their functional phenotypes, and whether their presence correlates with a specific disease endotype. To address this question, we identified a cohort of subjects with T1D who had diverse levels of residual beta cell function. Using previously developed HLA class II tetramer reagents, we enumerated T cells that recognize PTM GAD epitopes in the context of DRB1*04:01 or PTM IA2 epitopes in the context of DQB1*03:02 (DQ8). Consistent with prior studies, we observed higher overall frequencies and a greater proportion of memory T cells in subjects with T1D than in HLA matched controls. There were significantly higher numbers of GAD specific T cells than IA2 specific T cells in subjects with T1D. T cells specific for both groups of epitopes could be expanded from the peripheral blood of subjects with established T1D and at-risk subjects. Expanded neo-epitope specific T cells primarily produced interferon gamma in both groups, but a greater proportion of T cells were interferon gamma positive in subjects with T1D, including some poly-functional cells that also produced IL-4. Based on direct surface phenotyping, neo-epitope specific T cells exhibited diverse combinations of chemokine receptors. However, the largest proportion had markers associated with a Th1-like phenotype. Notably, DQ8 restricted responses to PTM IA2 were over-represented in subjects with lower residual beta cell function. Neo-epitope specific T cells were present in at-risk subjects, and those with multiple autoantibodies have higher interferon gamma to IL-4 ratios than those with single autoantibodies, suggesting a shift in polarization during progression. These results reinforce the relevance of PTM neo-epitopes in human disease and suggest that distinct responses to neo-antigens promote a more rapid decline in beta cell function.

Electro-acupuncture treatment ameliorates intestinal inflammatory injury in cerebral ischemia–reperfusion rats via regulating the balance of Treg / γδ T cells

Electro-acupuncture (EA) has an anti-inflammatory role in ischemic stroke, but whether the protective effect of EA involves the regulation of the intestine barrier and Treg/ γδ T cells is unclear. Cerebral ischemia–reperfusion (I/R) injury was induced by middle cerebral artery occlusion(MCAO) for 2 h followed by reperfusion for 24 h. The rats have treated with EA at the “Baihui” acupoint(GV20). Triphenyl tetrazolium chloride (TTC) staining and Longa neurologic score were performed to evaluate the outcomes after ischemic stroke. Inflammatory factor expression levels in the serum, ischemic hemisphere brain, and small intestine were detected by ELISA or RT-qPCR. Additionally, the morphology change of the small intestine was evaluated by analyzing villus height and smooth muscle thickness. Meanwhile, the expression of tight-junction proteins, including Zonula Occludens-1 (ZO-1), Occludin, and Claudin-1, were detected to evaluate the impact of EA on mucosal permeability in the small intestine. The percentages of regulatory T cells (Tregs) (CD45+CD4+Foxp3+) and γδ T cells (CD45+CD4-γδ T+) were measured to assess the effect of EA on intestinal T cells. EA decreased the brain infarction volume and intestine barrier injury in ischemic stroke rats. At the same time, it effectively suppressed the post-stroke inflammation in the brain and small intestine. More importantly, EA treatment increased the percentage of Tregs in the small intestine while reducing the rate of γδ T cells, and ultimately increased the ratio of Treg/ γδ T cells. These results demonstrated that EA ameliorated intestinal inflammation damage by regulating the Treg/ γδ T cell polarity shift and improving the intestine barrier integrity in rats with I/R injury. This may be one of the mechanisms underlying the anti-ischemic injury effects of acupuncture on stroke.

Spin-transport across a two-dimensional metal-semiconductor interface with infinite potential in presence of spin-orbit interactions: Double refraction and spin-filtering effect

The spin-transport across a two-dimensional metal-semiconductor junction with a Dirac-delta function potential at the interface and the Rashba and Dresselhaus spin-orbit interactions in the semiconductor region is studied exactly using discontinuous boundary conditions and the spin-polarized reflected and refracted current density and differential conductance are calculated. It is shown that in the presence of an infinite interface potential, an increase in the incident electron's energy reduces the spin splitting. It is also shown that the reflected and refracted coefficients, the spin-polarized currents and the corresponding differential conductance depend strongly on the spin-orbit interactions. The reflected spin polarization, however, becomes zero due to the infinite potential. The Rashba coupling enhances the refracted spin polarization while the Dresselhaus coupling reduces it. Thus, the maximum in polarization occurs at small values of Dresselhaus coupling and large values Rashba coupling. Interestingly, though the presence of delta-potential at the interface does not change the splitting angle between the spin-up and spin-down electrons, it causes a constant shift in the spin polarization.

MORGen: an Algorithm to Compute Spreading Centre and Transform Geometries from Simple Initial Plate Boundaries and Euler Rotations

The age structure of the global ocean floor is a key feature in paleogeographic reconstructions, which in turn forms the quantitative basis for Earth System Science. However, much of the ocean floor in paleogeographic reconstructions has been lost to subduction. The age structure of such lost ocean floor is constructed from the reconstructions of adjacent continents, using the relative rotations, around Euler poles to predict the geometry of spreading centres and transform faults. Building such mid-ocean ridge features in paleogeographic reconstructions is laborious, as it requires redrawing of ridge-transform systems upon every Euler pole shift in the model. In this paper, we present the Mid-Ocean Ridge Generator (MORGen) algorithm, based on pyGPlates. MORGen reduces the laborious task by automating the drawing of mid-ocean ridge geometries from geometrically simple plate boundary input assuming ridge-perpendicular spreading and adjusts ridge geometries in a simplest-scenario fashion by gradually adjusting ridge orientation and transform fault length upon Euler pole shifts, inspired by observations from the modern sea floor. The code takes as input curved line features, representing approximate divergent plate boundaries, and a set of Euler poles. These are then converted into spreading centre-transform geometries. Upon Euler pole shifts, the geometries are adjusted to fit the set of small circles and great circles dictated by the new Euler pole. For studies of paleo-environment and paleo-oceanography MORGen can be used in combination with other algorithms for full reconstructions of ocean floors, including their age, bathymetry, and roughness. For in-situ preserved ocean floor, the paleo-age distribution can be reconstructed directly in high resolution from geophysical and geological data from the modern ocean floor and MORGen would not normally be the option of choice. In cases where models contain ocean floor that has now been subducted, MORGen straightforwardly facilitates mid-ocean ridge geometry reconstruction. To illustrate how well the MORGen algorithm reproduces real ocean floor age structure, we show a synthetic ridge evolution for the South Atlantic and Southern Oceans and compare this to geophysically constrained ocean floor geometry. In addition, we show examples of use cases where direct (re)construction of mid ocean ridges is not possible: now-subducted ocean basins in the Mediterranean region and an ocean in a future supercontinent scenario.