Distinct Points Research Articles

A novel approach to engineering three-dimensional bladder tumor models for drug testing.

Bladder cancer (BCa) poses a significant health challenge, particularly affecting men with higher incidence and mortality rates. Addressing the need for improved predictive models in BCa treatment, this study introduces an innovative 3D in vitro patient-derived bladder cancer tumor model, utilizing decellularized pig bladders as scaffolds. Traditional 2D cell cultures, insufficient in replicating tumor microenvironments, have driven the development of sophisticated 3D models. The study successfully achieved pig bladder decellularization through multiple cycles of immersion in salt solutions, resulting in notable macroscopic and histological changes. This process confirmed the removal of cellular components while preserving the native extracellular matrix (ECM). Quantitative analysis demonstrated the efficacy of decellularization, with a remarkable reduction in DNA concentration, signifying the removal of over 95% of cellular material. In the development of the in vitro bladder cancer model, muscle invasive bladder cancer patients' cells were cultured within decellularized pig bladders, yielding a three-dimensional cancer model. Optimal results were attained using an air-liquid interface technique, with cells injected directly into the scaffold at three distinct time points. Histological evaluations showcased characteristics resembling in vivo tumors derived from bladder cancer patients' cells. To demonstrate the 3D cancer model's effectiveness as a drug screening platform, the study treated it with Cisplatin (Cis), Gemcitabine (Gem), and a combination of both drugs. Comprehensive cell viability assays and histological analyses illustrated changes in cell survival and proliferation. The model exhibited promising correlations with clinical outcomes, boasting an 83.3% reliability rate in predicting treatment responses. Comparison with traditional 2D cultures and spheroids underscored the 3D model's superiority in reliability, with an 83.3% predictive capacity compared to 50% for spheroids and 33.3% for 2D culture. Acknowledging limitations, such as the absence of immune and stromal components, the study suggests avenues for future improvements. In conclusion, this innovative 3D bladder cancer model, combining decellularization and patient-derived cells, marks a significant advancement in preclinical drug testing. Its potential for predicting treatment outcomes and capturing patient-specific responses opens new avenues for personalized medicine in bladder cancer therapeutics. Future refinements and validations with larger patient cohorts hold promise for revolutionizing BCa research and treatment strategies.

A "silent storm": uncovering the escalating crisis in mental healthcare for children and adolescents in Slovenia during and after the COVID-19 pandemic.

Our aim was to assess the burden of children and adolescents' mental health problems on the Slovenian outpatient healthcare system before, during and after the pandemic. In a retrospective analysis of healthcare indicators from 2008 to 2023, we analysed data from the National Institute of Public Health. Key domains included initial visits for mental and behavioural disorders (MBDs) to primary care for the population aged 0-19; the number of referrals to an initial assessment with a child and adolescent psychiatrist (CAP) at the secondary level for the population aged 0-17 along with the corresponding waiting times; and the number of urgent referrals for the population aged 0-17 to emergency mental health centres at the tertiary level. The calculations included rates per 1000 people. Descriptive statistics and diagrams were used to compare the data. Segmented linear regression analysis (SLR) was conducted on the primary healthcare data to identify the distinct temporal point indicating an increase. Comparing the average rates of the 2020-2022 period to those of the 2018-2019 period, there was a 20% increase in initial visits to primary care, a 23% increase in the referral rate to a CAP at the secondary level, and a 41% increase to the tertiary level of care. In secondary care, a four- to sevenfold increase in waiting times for the initial CAP assessment was observed between 2019 and 2023. The incidence of initial visits to primary healthcare services for MBD increased from 2008 to 2019 (average annual growth rate of 4.5%). The average annual growth rate for the 2020-2022 period tripled to 13.9%. The SLR showed that the trend of accelerating growth could have begun in 2017 for the 0-5 age group and possibly for the 15-19 age group as well. After the initial decline in 2020, all levels of the Slovenian healthcare system faced an increased burden of MBD in children and adolescents from 2021 to 2022 compared to pre-pandemic levels. Nevertheless, a portion of this increase aligns with longitudinal growing trends from 2008 onwards. Tackling the crisis requires urgent national action, significant improvement in organization, and investments in mental health.

Can Corporate Social Responsibility Shift Consumer Behavior? Insights from Scenario-Based Experiment in the Fast Fashion Industry

This study investigates the interplay between Corporate Social Responsibility (CSR) engagement and pricing strategies in shaping consumer purchase intentions in the fast fashion industry. Using a scenario-based experimental design with 267 participants, this research explores how different levels of CSR and two distinct price points influence purchasing behavior. Additionally, the moderating effects of individual differences, such as consumer wealth, motivations for sustainable behavior, and income, are examined. The findings indicate that higher levels of CSR engagement significantly enhance purchase intentions, particularly when combined with higher price points, perhaps due to consumer perceptions of CSR programs associated with more expensive brands as more authentic. However, price remains a critical factor for lower-income consumers, revealing an “ethical consumption gap” where affordability outweighs ethical concerns. Extrinsic motivations, such as social pressure, strongly influence wealthier consumers’ decisions, while intrinsic motivations show a more complex relationship with purchasing behavior. The findings provide practical insights for fast fashion brands, suggesting that aligning CSR with competitive pricing and authentic messaging is crucial for appealing to both price-sensitive and ethically conscious consumers.

Online Direct Infusion Mass Spectrometry of Liquid–Liquid Extraction Phases for Metabolite and Lipid Profiling with the Direct Infusion Probe

Background/Objectives: Profiling of metabolites and lipids in biological samples can provide invaluable insights into life-sustaining chemical processes. The ability to detect both metabolites and lipids in the same sample can enhance these understandings and connect cellular dynamics. However, simultaneous detection of metabolites and lipids is generally hampered by chromatographic systems tailored to one molecular type. This void can be filled by direct infusion mass spectrometry (MS), where all ionizable molecules can be detected simultaneously. However, in direct infusion MS, the high chemical complexity of biological samples can introduce limitations in detectability due to matrix effects causing ionization suppression. Methods: Decreased sample complexity and increased detectability and molecular coverage was provided by combining our direct infusion probe (DIP) with liquid–liquid extraction (LLE) and directly sampling the different phases for direct infusion. Three commonly used LLE methods for separating lipids and metabolites were evaluated. Results: The butanol–methanol (BUME) method was found to be preferred since it provides high molecular coverage and have low solvent toxicity. The established BUME DIP-MS method was used as a fast and sensitive analysis tool to study chemical changes in insulin-secreting cells upon glucose stimulation. By analyzing the metabolome at distinct time points, down to 1-min apart, we found high dynamics of the intracellular metabolome. Conclusions: The rapid workflow with LLE DIP-MS enables higher sensitivity of phase separated metabolites and lipids. The application of BUME DIP-MS provides novel information on the dynamics of the intracellular metabolome of INS-1 during the two phases of insulin release for both metabolite and lipid classes.

Personalized customer journeys for underserved communities: Tailoring solutions to address unique needs

In today's diverse marketplace, creating personalized customer journeys for underserved communities is essential for fostering inclusion and promoting equitable access to services. This review explores the significance of tailoring solutions to address the unique needs of these communities, emphasizing the role of personalized experiences in enhancing customer satisfaction and loyalty. Underserved communities often face systemic barriers, including limited access to resources, information, and services. Traditional customer journey models may overlook these specific challenges, leading to ineffective engagement and unmet needs. By adopting a personalized approach, businesses can better understand the distinctive characteristics, preferences, and pain points of these communities. This understanding allows for the development of targeted strategies that resonate with individuals' cultural, social, and economic contexts. The framework for personalized customer journeys involves several key components. First, comprehensive data collection is crucial for identifying the unique demographics and psychographics of underserved communities. Utilizing qualitative and quantitative research methods, businesses can gather insights on customer behaviors, preferences, and challenges. Next, leveraging advanced analytics and artificial intelligence can enhance the personalization of interactions, ensuring that offerings align with the specific needs and aspirations of these communities. Additionally, fostering collaboration with community organizations and leaders is vital. Engaging with local stakeholders helps businesses build trust and credibility, facilitating the co-creation of solutions that genuinely address the community's needs. This collaborative approach can lead to the development of tailored products and services, targeted marketing strategies, and supportive customer service frameworks. Ultimately, personalized customer journeys for underserved communities not only improve customer satisfaction but also drive business growth and social impact. By prioritizing equity and inclusion, businesses can enhance their brand reputation, foster customer loyalty, and contribute to the economic empowerment of these communities. In conclusion, a commitment to understanding and addressing the unique needs of underserved communities through personalized customer journeys is essential for fostering inclusive growth and creating lasting positive change.

Quantitative magnetooptical analysis using indicator films for the detection of magnetic field distributions, temperature, and electrical currents

The accurate characterization of local magnetic fields and temperature is vital for the design of electronic systems. To meet this imperative, we present a novel non-contact approach for simultaneous quantitative magnetic field imaging and temperature sensing using magnetooptics and a bismuth-doped yttrium iron garnet film with out-of-plane anisotropy. For the direct signal quantification, a Stokes polarization camera is employed in a conventional magnetooptical microscope. The magnetization in the garnet is modulated with an external magnetic field to continuously image the Faraday rotation at four distinct points along the saturating magnetization loop. The method enables sensing of the magnetooptical signal in saturation, the magnetooptical susceptibility, the temperature, and self-calibrated driftfree imaging of the out-of-plane magnetic field component. A spatial resolution of magnetic field in the micrometer range with millisecond exposure time is demonstrated. The method is verified by analyzing the stray magnetic field distribution of electrical current in a wire simultaneously to the Joule heating induced by the applied current.

Prediction of Potential Suitable Distribution Areas for Northeastern China Salamander (Hynobius leechii) in Northeastern China

The Northeastern China Salamander (Hynobius leechii) is classified as a rare, nationally protected Class II wild animal in China. Its population is declining, and its habitat is deteriorating. This study aimed to predict the distribution of suitable habitats for the Northeastern China Salamander under both current and future climate scenarios, utilizing the MaxEnt model optimized through ENMeval parameters. Species distribution data were collected from field surveys, existing literature, amphibian records in China, and the Global Biodiversity Information Network. A total of 97 records were compiled, with duplicate records within the ENMTools grid unit removed, ensuring that only one record existed within every 5 km. Ultimately, 58 distinct distribution points for the Northeastern China Salamander were identified. The R software package ‘ENMeval 2.0’ was employed to optimize the feature complexity (FC) and regularization multiplier (RM), and the optimized model was applied to assess the suitable distribution regions for the Northeastern China Salamander under present and future climate conditions. The findings indicated that rainfall and temperature are the primary environmental factors influencing Hynobius. Currently, the suitable habitat for the Northeastern China Salamander constitutes 6.6% of the total area of Northeastern China. Projections for the periods of 2050 and 2070 suggest that suitable habitats for the Northeastern China Salamander will continue to expand towards higher latitudes across three climate scenarios. While this study focuses solely on climate change factors and acknowledges certain limitations, it serves as a reliable reference and provides essential information for the distribution and conservation of the Northeastern China Salamander.

Development of β‐Carboline‐Coumarin Based Hybrids as Potential Cytotoxic and Topoisomerase IIα Inhibitors

AbstractCancer remains a global health concern, prompting extensive efforts to develop novel inhibitors targeting the enzyme topoisomerase IIα as potential anti‐cancer agents. Herein, we strategically combined the pharmacophores from natural (β‐carboline) and (coumarin) sources, diversifying them at two distinct points. The in vitro evaluation of cytotoxic properties were estimated on human cancer cell lines, reveals that among the tested compounds, 13 r (isopropyl‐substituted β‐carboline) exhibited remarkable potency with IC50 value of 4.37 μM in the HCT116 cell line. Its selectivity for cancer cells was validated against normal keratinocyte cells, establishing a favourable selectivity index. Further, SAR studies indicate that no substitution (13 r) at C‐6 and C‐7 of β‐carboline, enhance anticancer activity. Assays confirmed its ability to intercalate with DNA, inhibit topoisomerase IIα, induce apoptosis, and disrupt mitochondrial membrane potential in HCT116 cells. Flow cytometric assays gauged the induction of apoptosis using Annexin‐V/PI dual staining and the disruption of mitochondrial membrane potential through JC staining. Results demonstrated that compound 13 r induced apoptosis and dose‐dependent depolarization of the mitochondrial membrane in HCT116 cells. Molecular docking studies further validated that compound 13 r binds within the active site of DNA when complexed with topo IIα, and this binding was stabilized through interactions with DNA base pairs and amino acid residues.

Dating Violence Continuity: The Protective Role of Self-Esteem Against Revictimization by Multiple Partners.

Dating violence is a deleterious relational dynamic that affects around one-third of adolescents. It is well documented that dating violence can persist over time, occurring within the same relationship or across multiple relationships. Self-esteem could be a key factor in understanding the mechanisms of violence continuity, potentially acting as a protective factor during adolescence. Exploring its role in the continuity of dating violence and the potential differences based on whether the violence occurs within the same relationship or across different relationships could inform interventions aimed at preventing repetitive violence and revictimization by multiple partners. This study aimed to (1) examine the association between dating violence measured at two distinct time points, (2) investigate the moderating role of self-esteem in this association, and (3) explore potential variations in the protective role of self-esteem based on whether the violence reoccurred within the same relationship or across different relationships. Sex differences were also examined. A subsample of 1,018 adolescents who reported being involved in a dating relationship at two time points completed self-reported questionnaires. A moderation analysis revealed that dating violence at Time 1 predicted dating violence at Time 2 (6 months later) for both boys and girls. Among girls, self-esteem acted as a protective factor when considering the effect of partner change. Hence, girls who experienced dating violence victimization at Time 1 but had higher self-esteem were more inclined to engage in a subsequent relationship devoid of dating violence at Time 2. However, self-esteem did not emerge as a significant protective factor among boys. Self-esteem appears to be a pivotal factor in promoting engagement in subsequent positive relationships following experiences of dating violence among girls. These findings offer valuable insight that can inform preventive measures and interventions, ultimately contributing to healthier relationship dynamics and improving the well-being of dating violence victims during adolescence.

Operationalism, Science and Technology

Bridgman’s operationalism incurred serious difficulties, and this fact induced many authors to dismiss operationalism per se. Developed beginning in the late 1960s, Agazzi’s operationalism escaped these difficulties from the beginning, but the persistence in it of a traditional primacy attributed to theory over experiment led him to pose an opposition between science and technique that was inconsistent with the general operationalist framework of his philosophy of science. Later, Agazzi sought to make his operationalism more coherent by moving toward a closer relationship between science and technology, that is, toward an intermediate solution which sees them as interacting with one another. However, in order to coherently defend an intermediate solution, it is not enough to find a certain set of properties that would be peculiar to science but not to technique, or vice versa. Rather, it is necessary to specify two distinct (but also complementary) points of view, from which science and technique can appear, at the same time but without contradiction, both as conceptually distinct and as indistinguishable as historical-empirical realities: in one sense, science is irreducible from technology, but in the other sense, they coincide completely.

Ultrasound-Based Phenotyping for Genetic Selection of Carcass Traits in Oreochromis niloticus: Integrating Imaging Technology Into Aquaculture Breeding.

Recent years have witnessed a remarkable global surge in fish production, with Nile tilapia (Oreochromis niloticus) emerging as a prominent contributor owing to its high demand as a nutritious food source. However, unlike terrestrial species, maintaining genealogical control and collecting phenotypic data in fish farming poses significant challenges, necessitating advancements to support genetic improvement programmes. While conventional methods, such as body measurements using rulers and photographs are prevalent in data collection, the potential of ultrasound-a less invasive and efficient tool for fish measurement-remains underexplored. This study assesses the viability of ultrasonography for genetically selecting carcass characteristics in Nile tilapia. The investigation encompasses data from 897 animals representing 53 full-sib tilapia families maintained in the genetic improvement programme at the Federal University of Lavras. To measure carcass traits, the animals were sedated with benzocaine and ultrasound images were obtained at three distinct points. Subsequently, the animals were euthanised through medullary sectioning for further carcass processing. After evisceration, filleting and skinning, all weights were meticulously recorded. (Co)variance components and genetic parameters of the measured traits were estimated using the Bayesian approach by Gibbs sampling implemented in MTGSAM (Multiple Trait Gibbs Sampling in Animal Models) software. Heritabilities estimated for the studied carcass traits were moderate, ranging from 0.23 to 0.33. Notably, phenotypes derived from ultrasound images demonstrated substantial genetic correlations with fillet yield (0.83-0.92). In conclusion, this study confirms that indirect selection based on ultrasound images is effective and holds promise for integration into tilapia breeding programmes aimed at enhancing carcass yield.

Partial biochemical and immunological characterization of an isolated 52.1 kDa pregnancy-associated glycoprotein charge variant

Pregnancy-associated glycoproteins (PAGs) are synthesized in the placental cells of ruminants and are detectable in blood, milk, and urine. Many of these proteins have been obtained and characterized from placental extracts by precipitation with 80 % ammonium sulfate. The possibility of purifying PAGs by precipitation with other concentrations of ammonium sulfate remains unexplored. We aimed to study PAG proteins obtained from extracts of ovine placenta at 100 days of gestation through precipitation with 40 % ammonium sulfate (Extract 40). The main protein complex (130 kDa) was obtained after Extract 40 precipitation. Under reducing SDS-PAGE conditions, the 130 kDa complex dissociated in two PAG proteins with apparent molecular weights of 52.1 kDa and 26.1 kDa. The 130 kDa protein appeared to be a molecular complex consisting of two copies of the 52.1 kDa protein linked to one copy of the 26.1 kDa protein, presumably by disulfide bonds. Furthermore, the 52.1 kDa protein consisted of at least three isoforms with distinct isoelectric points. Amino acid microsequencing of the 52.1 kDa protein revealed a chimeric structure containing amino acid sequences of PAG1, PAG4, PAG6, and PAG1-like proteins. This procedure recovered a novel 130 kDa protein complex composed of 26.1 kDa and two 52.1 kDa PAGs. To the best of our knowledge, this has not been previously reported as heterologous polymeric molecules.

GASCOM: Graph-based Attentive Semantic Context Modeling for Online Conversation Understanding

Online conversation understanding is an important yet challenging NLP problem which has many useful applications (e.g., hate speech detection). However, online conversations typically unfold over a series of posts and replies to those posts, forming a tree structure within which individual posts may refer to semantic context from elsewhere in the tree. Such semantic cross-referencing makes it difficult to understand a single post by itself; yet considering the entire conversation tree is not only difficult to scale but can also be misleading as a single conversation may have several distinct threads or points, not all of which are relevant to the post being considered. In this paper, we propose a Graph-based Attentive Semantic COntext Modeling (GASCOM) framework for online conversation understanding. Specifically, we design two novel algorithms that utilize both the graph structure of the online conversation as well as the semantic information from individual posts for retrieving relevant context nodes from the whole conversation. We further design a token-level multi-head graph attention mechanism to pay different attentions to different tokens from different selected context utterances for fine-grained conversation context modelling. Using this semantic conversational context, we re-examine two well-studied problems: polarity prediction and hate speech detection. Our proposed framework significantly outperforms state-of-the-art methods on both tasks, improving macro-F1 scores by 4.5% for polarity prediction and by 5% for hate speech detection. The GASCOM context weights also enhance interpretability.

Optimized design of a fault-tolerant 12-slot/10-pole six-phase surface permanent magnet motor with asymmetrical winding configuration for electric vehicles

This paper presents a comprehensive methodology for optimizing the design of a 12-slot/10-pole permanent magnet (PM) motor with a six-phase winding configuration tailored for electric vehicles (EVs). The design aims to enhance motor performance under both healthy and fault conditions. While the single neutral configuration offers superior torque during faults, it also introduces zero sequence currents and additional space harmonics, which can lead to increased torque ripple that is difficult to control. This study addresses these challenges through innovative machine design optimization. The optimization process begins with sizing equations to establish an initial design. K-means clustering techniques are then employed to identify distinct loading points that accurately represent the full EV driving cycle, effectively minimizing computational power requirements. Following this, the Full Range Minimum Loss (FRML) strategy is applied to determine optimal current profiles across these loading points, significantly reducing copper losses. Finally, a multi-objective optimization approach is utilized to minimize torque ripple, enhance average torque, and optimize machine losses. The results demonstrate substantial improvements in torque and reduced ripple, validated through experiments conducted with a 2 kW lab-scale motor. This integrated approach not only ensures a robust and efficient motor design but also enhances fault tolerance, making it well-suited for advanced EV applications.

Exploring the Relationship between Hygiene Factors and Job Satisfaction in Workplace

The purpose of this research is to investigate the relationship that exists between hygiene factors and job satisfaction (JS) among the Royal Malaysian Navy (RMN) personnel in the workplace. The focus shall be directed toward the diligent personnel on board at Lumut Naval Base. The present study adopts a quantitative analysis methodology, employing a straightforward descriptive approach and correlation analysis through SPSS 23, wherein a sample of 220 employees was randomly selected for analysis. The empirical evidence suggests that there exist notable associations between job satisfaction (JS) and various factors, namely company policy, salary and benefits, and interpersonal relationships. The variables of job security and working conditions do not exhibit a statistically significant correlation with job satisfaction. The findings of this study suggest that there exists a noteworthy correlation between variables towards Job Satisfaction (JS). Therefore, there is no significant correlation between Job Security and Working Conditions in JS. The study's impact extends beyond the organization, encompassing the broader field, as it adds to the body of literature about the research elements. The utilization of models and theoretical frameworks in prospective investigations presents a distinct point for comprehending and interpreting phenomena that represents a noteworthy innovation within the context study.

Curvature continuous corner cutting

Subdivision schemes are used to generate smooth curves by iteratively refining an initial control polygon. The simplest such schemes are corner cutting schemes, which specify two distinct points on each edge of the current polygon and connect them to get the refined polygon, thus cutting off the corners of the current polygon. While de Boor (1987) shows that this process always converges to a Lipschitz continuous limit curve, no matter how the points on each edge are chosen, Gregory and Qu (1996) discover that the limit curve is continuously differentiable under certain constraints. We extend these results and show that the limit curve can even be curvature continuous for specific sequences of cut ratios.

Preparation and Characterization of Schiff Base Ligand Complexes (Dimedone With Thiourea)

In this study, eight complexes of this group were prepared by reacting the new ligand prepared from the reaction of dimedone with thiourea with metal chlorides of divalent (Mn, Co, Ni, Cu ,Zn, Cd) and trivalent (Fe, Cr) transition elements. These complexes were characterised by distinctive colours, melting points and solubility, and from the diagnostic results obtained, it was found that the ligand.(1E,5E)-5-carbamothioylimino)-3,3-dimethylcyclohexylidene) thiourea(L2) , that behave as dodecameric ligands with the metal ion, to which they bind through two amine groups. The octahedral shape of the complexes was suggested by IR, UV-visible, molar electrical conductivity, and magnetic susceptibility spectra, all of which indicated paramagnetic properties except for Zn and Cd, which have diamagnetic properties, and molar conductivity showed that all complexes are non-electrolytic except for Fe and Cr. The molecular formula of the complexes forming the group was determined by elemental analysis, where the elemental percentages matched the proposed molecular formula. The results measured the biological efficacy against pathogenic bacteria of the complexes showed varying efficacy. The results of antioxidant efficacy measurement using ethanol showed varying efficacy and were compared with gallic acid using DPPH as a free radical and gave different IC50 values. Density Functional Theory (DFT) measurement results showed that the chemical properties of the prepared complexes were consistent with the results of the practical part.

Assessment of Cochlear Implant Rehabilitation Success by Speech-Language Therapists Using International Classification of Functioning, Disability and Health Criteria.

This study aims to introduce an assessment questionnaire based on the International Classification of Functioning, Disability and Health (ICF) to enable quantifiable clinical documentation. The questionnaire assists speech-language therapists (SLTs) in evaluating both non-audiological and audiological rehabilitation outcomes of patients with cochlear implants (CIs). Six SLTs were recruited to evaluate the effectiveness of CI care using ICF criteria. We carefully selected 20 pertinent ICF criteria, and SLTs received thorough training in how to apply them uniformly. A cohort of 48 patients was evaluated at three distinct time points: presurgery, postsurgery, and 6 months post-initial CI fitting. Patients underwent dedicated speech-language therapy sessions throughout the treatment course. A total of 144 complete datasets were scrutinized using a two-factor analysis of variance for ranks. Following CI care, patients exhibited substantial improvements in auditory perception, characterized by enhanced sound recognition and speech discrimination. Additionally, patients demonstrated improved attentional focus, proficiency in daily tasks and increased conversational engagement over time. Environmental factors, particularly sound perception, improved markedly, correlating with reported reductions in stress levels. Minimal changes were noted in stress management and communication techniques. Articulatory function, speech functions, and pain perception exhibited the least alteration. The data extracted from the ICF-based assessments underscore the vital significance of incorporating speech therapy-based metrics to evaluate both non-audiological and audiological factors in assessing the success of CI care within the SLTs domain. This approach proves to be highly relevant both in terms of demonstration and clinical applicability.

The extension of mammalian pregnancy required taming inflammation: Independent evolution of extended placentation in the tammar wallaby.

In the first live-bearing mammals, pregnancy was likely short and ended with a brief period of inflammatory maternal-fetal interaction. This mode of reproduction has been retained in many marsupials. While inflammation is key to successful implantation in eutherians, a key innovation in eutherians is the ability to switch off this inflammation after it has been initiated. This extended period, in which inflammation is suppressed, likely allowed for an extended period of placentation. Extended placentation has evolved independently in one lineage of marsupials, the macropodids (wallabies and kangaroos), with placentation lasting beyond the 2 to 4 d seen in other marsupial taxa, which allows us to investigate the role of inflammation response after attachment in the extension of placentation in mammals. By comparing gene expression changes at attachment in three marsupial species, the tammar wallaby, opossum, and fat-tailed dunnart, we show that inflammatory attachment is an ancestral feature of marsupial implantation. In contrast to eutherians, where attachment-related (quasi-) inflammatory reaction is even involved in epitheliochorial placentation (e.g., pig), this study found no evidence of a distinct attachment-related reaction in wallabies. Instead, only a small number of inflammatory genes are expressed at distinct points of gestation, including IL6 before attachment, LIF throughout placentation, and prostaglandins before birth. During parturition, a more distinct inflammatory reaction is detectable, likely involved in precipitating the parturition cascade similar to eutherians. We suggest that in wallaby, extended gestation became possible by avoiding an inflammatory attachment reaction, which is a different strategy than seen in eutherians.

Dynamic Music Video: Categorizing the Convergence of Video Games and Music Videos

The present article examines how the aesthetics of a specific format can be applied to another, thus creating a new autonomous product. This is the case of a distinctive point in video games wherein the playthrough is imbued with music video aesthetics, and the player interacts with the music, camera movements and landscape, becoming the protagonist and architect of what I propose to define as a “Dynamic Music Video”—following Collins’ definition of “dynamic music” (2008, 139). This study analyzes the musical transmedia narratives created from Death Stranding and other contemporary games, both regarding the gameplay experience and the User-Generated Content, focusing on their bidirectional motion: one that converts the players’ experience into a music video inserted into a video game, and one that converts the video game into an autonomous music video.