Soil tillage may influence CO 2 emissions in agricultural systems. Agricultural soils are managed in several ways in Brazil, ranging from no tillage to intensive land preparation. The objective of this study was to determine the effect of common soil tillage treatments (disk harrow, reversible disk plow, rotary tiller and chisel plow tillage systems) on the intermediate CO 2 emissions of a dark red latosol, located in southern Brazil. Different tillage systems produced significant differences in the CO 2 emissions, and the results indicate that the chisel plow produced the highest soil carbon loss during the 15 days period after tillage treatments were performed. Emissions to the atmosphere increased as much as 74 g CO 2 m −2, at the end of a 2-week period, in the plot where the chisel plow treatment was applied, in comparison to the non-disturbed plot. The results indicate that the total increase on the intermediate term soil CO 2 emissions due to tillage treatments in southern Brazil is comparable to that reported for the more humid and cooler regions.