Pleurotus Research Articles

Intelligent System Design for Oyster Mushroom Cultivation Using Mamdani Fuzzy Inference with Internet of Things (IoT)

Oyster mushroom (Pleurotus ostreatus) has great potential in agricultural development with increasing global market demand. This research develops an intelligent cultivation system based on Mamdani fuzzy and IoT to control air temperature, air humidity, and soil moisture automatically and in real-time, using DHT22 sensor, resistive soil moisture sensor, and ESP32 as microcontroller. The test results show a high level of accuracy, with an average sensor error of 1% for air temperature, 3.8% for air humidity, and 9.3% for soil moisture. The validation of watering, humidification, and fan prediction times compared to MATLAB calculations is excellent, with MAPE values of 1.52%, 1.43%, and 1.43%, respectively. This system offers a more accurate and responsive automated solution compared to conventional cultivation management. Further testing on actual oyster mushroom farms is required to determine whether the system can adapt to varying environmental conditions. The system relies heavily on a stable internet connection to optimally perform the real-time Mamdani fuzzy calculation function.

Biological active compounds from edible mushroom: Effect on hela cells

Background: Mushrooms are unique organisms rich in proteins, carbohydrates, vitamins, and minerals, which allows them to be used not only as a source of nutrition but also in cosmetics and medicine. The fungus P. ostreatus possesses two strong enzymatic complexes – lignin-decaying and cellulose-decaying. Objective: The research aimed to study the antitumor activity of treated mm-wave mycelial extracts of mushrooms on the HeLa cell line. Research on edible mushrooms against tumors is very actual and important for the field of functional food science and bioactive compounds field. Methods: The effect of the fungal culture extract on the cyto-photometric and morphometric indices of the HeLa cell line culture was studied according to the nature of proliferation and transcription of malignant tissues. Using high-resolution liquid chromatography, the amino acid composition of acid-soluble proteins in mycelial extracts of a mushroom culture treated with mm waves was studied. Results: Extracts of P. ostreatus mycelial culture significantly suppressed the growth of proliferative activity of human tumor cultures and completely suppressed the mitotic activity of continuous HeLa cell cultures to 72 hours. Our study revealed a significant increase in glutamic and aspartic acids in mycelial culture extracts treated by mm-waves. Conclusion: Based on HPLC analysis, we suggest polyfunctional peroxidase as the candidate for the role of biologically active compound in the composition of aqueous extracts of the mycelial culture. So, the polyfunctional peroxidase plays an active role in the therapeutic properties of the oyster mushroom. Keywords: anticancer activity, ant-inflammation activity, mm-waves, mushroom`s, mycelial extracts, HPLC of acid-soluble proteins, HeLa cell line.

Social status of oyster mushroom enterprisers in Chandrapur district of Maharashtra

Social status of oyster mushroom enterprisers in Chandrapur district of Maharashtra

Enhancing oyster mushroom cultivation by chickpea and wheat straw substrate for sustainable agriculture

This study aimed to explore the potential utilization of agro-lignocellulosic waste materials for oyster mushroom cultivation, addressing the challenge of managing waste associated with these materials. Various combinations of Chickpea Straw (CS) and Wheat Straw (WS) at different ratios-100% CS, 75% CS + 25% WS, 50% CS + 50% WS, 25% CS + 75% WS, and 100% WS-were investigated as substrates for mushroom cultivation. The experiment followed a ccompletely rrandomised design with four replications, monitoring developmental phases, yield, and biological efficiency (BE). Results indicated that using 100% WS as a substrate resulted in the fastest mycelium growth (spawn run), completing in an average of 14.50 days, 17.20 days from spawning to pinhead formation, and 20.60 days to first harvest, with the highest number of fruiting bodies produced. Chickpea straw contributed to the highest stipe width (1.52 cm), while WS 100% had the highest average yield (997.28 g) and BE (99.73%). A mixture of 75% CS and 25% WS showed promising results in terms of fruiting bodies, yield, and BE after 100% WS. Consequently, the study recommends the use of these substrates to optimize Pleurotus ostreatus cultivation yield.

SOSIALISASI PENGOLAHAN JAMUR TIRAM MENJADI NUGGET SEBAGAI UPAYA PENINGKATAN EKONOMI MASYARAKAT DESA TAMANSARI WONOREJO PASURUAN

White oyster mushroom (Pleurotus ostreatus) is a type of fungus that often grows on agricultural waste in the form of wood or its derivatives. Fungi obtain food from places where they grow and can survive on plant remains around other organisms. Oyster mushrooms are a food commodity that is in great demand among the public, so by cultivating mushrooms it is hoped that they will be able to be involved further, it is hoped that creativity and innovation will emerge and be able to provide added value to society. Oyster mushrooms are only produced and sold fresh without any diversification process. In this KKN activity there will be training on oyster mushrooms to increase family income. As a result of this training, PKK women from Tamansari Wonorejo Village were able to provide understanding and skills to activity participants. PKK women in Tamansari Wonorejo Village have knowledge and skills regarding the food potential around them as an effort to increase family income.

Effect of Cold Exposure on the Biofoam Produced from Different Types of Oyster Mushroom

Mycelium-based biofoam is a sustainable material derived from the growth of fungal mycelium on lignocellulosic agricultural waste substrate, as it has potential use in a variety of applications. The main objective of this research is to advance the sustainable alternatives for various application by investigating the mycelium growth of the biofoam produced from Pleurotus florida and Pleurotus sajor-caju on rice husk substrate, in improving the properties of the biofoam through innovative cold exposure. This study showed P. florida can produce mycelium biofoam at a faster rate, 7.022mm/day compared to P. sajor-caju 6.08mm/day). By cold exposure at 0°C and 10°C for 3 hours, every 2 days and 5 days, respectively until the mycelium are fully grown in the substrate, sample exposed to the latter condition for P. florida exhibits a faster growth rate at 7.3037 mm/day. However, cold exposure on biofoam produced from P sajor-caju had not improved the mycelium growth rate. Cold exposure samples at 0°C every 5 days and 10°C every 2 days have demonstrated capability in water (103.51%) and oil absorption (143.23%), proving their effectiveness in absorbing pollutants for the purpose of environmental remediation. The FTIR analysis confirmed the presence of hydrophilic and oleophilic characteristics in the biofoam, indicating its capability to absorb water and oil. By subjecting biofoam to cold exposure, its properties can be altered, broadening its potential applications.

Green mould contamination of Pleurotus pulmonarius cultivation in Malaysia: Unravelling causal agents and water source as critical factors

Green mould contamination causes a significant challenge to mushroom growers in Malaysia leading to reduced yields and economic losses in the widely cultivated and marketed edible grey oyster mushroom, Pleurotus pulmanorius. This study aimed to identify the causal agents of green mould contaminants and determine the critical points in the cultivation process in the farm that contribute to green mould contamination. Samples of mushroom substrate (sawdust), spawn substrate (corn), environmental sources and tools were collected at different stages of mushroom cultivation. As results, the causal agents of green mould contamination were identified as Trichoderma pleuroti, T. harzianum and T. ghanese. Prior to steam pasteurisation and after steam pasteurisation, the spawn substrate and mushroom substrate were found to be free of Trichoderma. However, Trichoderma was detected in water, air within the production house and on cleaning tools. This findings suggests that water could serve as the source of green mould introduction in mushroom farms, while cultivation practices such as watering and scratching during the harvesting cycle may contribute to adverse green mould. Understanding these critical points and causal agents provides information to mitigate the green mould contamination throughout the grey oyster mushroom cultivation process.

Comparative Effect of Oyster and Button Mushroom Loaded Phytosomes with Glibenclamide on Renal Function in STZ Induced Diabetic Rats.

Diabetes is a chronic, life-threatening condition that can affect a vast variety of organ systems, including the kidney. The present study is based on the effect of phytosome-based mushroom extract of oyster and button mushroom on renal functioning factors such urea, uric acid, and creatine as well as the glucose level in streptozocin (STZ) -induced rats with diabetes. The results indicated the blood sugar level was found to decrease significantly in the groups treated with a combination of lactobacillus with phytosomes of oyster mushroom and button mushroom in contrast to a control group of diabetics (p

Comparative Evaluation of Chemical Compositions and Antioxidant Activity in Fruiting Bodies and Mycelia of Volvariella volvacea and Pleurotus sajor-caju Extracts.

Mushrooms, recognized for their rich medicinal and nutritional content, have garnered significant interest due to their potent antioxidant properties. This study investigates the fruiting bodies and mycelia of straw mushrooms (Volvariella volvacea) and grey oyster mushrooms (Pleurotus sajor-caju). The mushrooms were extracted using distilled water at ratios of 1:25, 1:30, and 1:35 g/mL by soaking in a temperature-regulated shaking bath set at 50°C. The aqueous extracts were analyzed for their bioactive components, including polysaccharides, proteins, GABA, total phenolic content (TPC), and total flavonoid content (TFC), along with their antioxidant capacities using DPPH and ABTS assays. The results indicated that the straw mushroom exhibited higher levels of polysaccharides, GABA, TPC, and TFC compared to the grey oyster mushroom, which contained higher protein levels. Specifically, the fruiting bodies extract of the straw mushroom demonstrated the highest levels of polysaccharides (416.94 mg/g extract), GABA (12.86 mg/g extract), TPC (33.27 mg GAE/g extract), and TFC (9.39 mg CE/g extract). Furthermore, the fruiting bodies extract showed a greater capacity to scavenge free radicals than the mycelia extract of both straw mushroom and grey oyster mushroom, with DPPH assay values of 0.65 and 0.47 mg TE/g extract and ABTS assay values of 2.26 and 1.74 mg TE/g extract, respectively. These results suggest that the fruiting bodies possess higher free radical scavenging potential as an outcome of their high levels of bioactive compounds. Consequently, these extracts indicate promise as natural antioxidative agents and could be effectively utilized as ingredients in the culinary industry.

Применение биоугля на основе отработанных грибных блоков в условиях агротемно-серых почв

Recycling of organic waste from mushroom production will improve their environmental safety and optimize the applied agricultural technologies. The aim of the work was to assess the environmental performance of the agricultural technology of using a soil fertilizing material – biochar obtained from spent lignin-cellulose mushroom blocks. It was found that the introduction of biochar from mushroom production waste materials into Gray-Luvic Phaeozems Hortic helps stabilize soil organic matter and increase the productivity of lawn grasses. It was noted that the quality and agroecological efficiency of biochar obtained from spent mushroom blocks based on cereal straw are significantly higher than biochar from spent mushroom blocks based on sunflower husk. Keywords: SOIL ORGANIC MATTER, CO2 EMISSION, SUNFLOWER HUSK, LAWN GRASSES, OYSTER MUSHROOM

The Effect of Trust, Communication and Commitment on Relationship Quality and its Impact on Oyster Mushroom Supply Chain Performance in Kuningan Regency

The Effect of Trust, Communication and Commitment on Relationship Quality and its Impact on Oyster Mushroom Supply Chain Performance in Kuningan Regency

Additive efficacy of natural versus synthetic feed additive on cellular immunity and egg quality in layers

Feed additives are the products used in animal diets for the purpose of improving the quality of feed and the quality of food from animal origin or to improve animal performance and health. The present study was planned to evaluate the beneficial effect of two edible dried mushrooms, probiotics, antioxidant and antibiotic on layer performance, cellular immunity, and antioxidant activities under hot climatic conditions. In this trial, three hundred W-98 commercial hens of 35th week of age were randomly assigned to six dietary treatments, replicated five times with 10 hens per replicate for 12 weeks. Dietary treatments were as follows; antibiotic (enramycin) 50g/ 50 kg, probiotics 50g/ 50 kg, antioxidant (ethoxyquine) 60 ppm, organic acid 50g / 50 kg; Oyster mushroom and button mushroom 1% of feed bag. The data revealed that initial, final body weight, egg production (%) and feed intake, egg specific gravity, egg shape index, egg yolk index and shell thickness and livability were non-significant (p<0.05) in all treatments. Egg weight, feed to egg mass ration, Haugh unit and damaged eggs were significantly (p>0.05) higher among different treatment groups. Total anti-oxidative capacity was significantly (p>0.05) higher in ethoxyquine, followed by oyster mushroom, button mushroom, negative control (without any feed additives), probiotics and enramycin (positive control / antibiotic). Antioxidant parameters like super oxide dismutase was significantly (p>0.05) higher in ethoxyquine followed by probiotics, oyster mushroom, button mushroom, negative control, and positive control treatments groups. Immunity parameters like catalase (interferon gamma) was non-significant (p>0.05) in all treatment’s groups. IFN-γ (Interferon-gamma) was significantly (p<0.05) higher in oyster mushroom and ethoxyquine followed by probiotics, button mushroom, negative control (without any feed additives) and positive control (with antibiotic). IL-10 (Interleukin-10) was significantly (p<0.05) higher in button mushroom, oyster mushroom and probiotics followed by ethoxyquine, negative control (without any feed additives) and positive control (with antibiotic). On whole mushrooms are effective feed additives in poultry production IFN-γ (Interferon-gamma) was significantly (p<0.05) higher in oyster mushroom and ethoxyquine followed by probiotics, button mushroom, negative control (without any feed additives) and positive control (with antibiotic). IL-10 (Interleukin-10) was significantly (p<0.05) higher in button mushroom, oyster mushroom and probiotics followed by ethoxyquine, negative control (without any feed additives) and positive control (with antibiotic). On whole mushrooms are effective feed additives in poultry production

PENINGKATAN PRODUKTIVITAS DAN EKONOMI KELOMPOK BUDIDAYA JAMUR TIRAM PUTIH (Pleurotus ostreatus) MELALUI TEKNOLOGI PENGATUR SUHU DAN KELEMBABAN KUMBUNG BERBASIS INTERNET OF THINGS (IoT)

This community service program studies the application of Internet of Things-based kumbung temperature and humidity regulation technology for kumbung in the cultivation of white oyster mushrooms in Tasiwalie Village, Pinrang Regency. The aim is to stimulate oyster mushroom cultivation groups to adopt modern technology to increase productivity and economic demand. This method involves a deep understanding of needs groups and the exploration of effective technology solutions. The results show that this technology drives positive changes in cultivation practices, increasing productivity and economic prosperity. With a focus on sustainability, the application of this technology provides long-term benefits for cultivation groups. The implication is that investment in this technology can be a strategy for developing the white oyster mushroom cultivation sector and make a positive contribution to the local economy.

EFFECTS OF ADDITION BAGASSE DEGRADANT BY CELLULOLYTIC BACTERIA Bacillus subtilis ON GROWTH MEDIA OF OYSTER MUSHROOM Pleurotus ostreatus

Background: Sugarcane (Saccharum officinarum L.) serves as a valuable resource for the sugar and beverage industries, a significant quantity of its by-product, bagasse, remains underutilized. A promising strategy for increasing the value of bagasse involves improving its substrate quality through the application of cellulose-degrading bacteria, such as Bacillus subtilis. Methods: The objective of this research is to investigate the influence of bagasse degradant supplementation on the growth of oyster mushrooms. A randomized experimental design was employed to evaluate growth media containing varying concentrations of bagasse degradant (500, 600, 700, and 800 gram) in comparison to a control group. The study assessed the impact of bagasse degradant on pin diameter, fresh weight, dry weight, and water content, using ANOVA and the Duncan's Multiple Range Test (DMRT) at a significance level of 5%. Results: The study showed the addition of bagasse degradant affected the pin diameter, fresh weight, and dry weight of oyster mushroom. However, it is not effective towards the water content of oyster mushroom. Conclusion: The addition of bagasse degradant affected the pin diameter, fresh weight, and dry weight, whereas the water composition did not affected the growth of P. ostreatu

Exploring agricultural waste substrate for enhancement of <i>Pleurotus ostreatus</i> cultivation

The proximate composition of the organic waste substrates used in cultivating Pleurotus ostreatus was analyzed, and the results revealed significant variations in various nutritional components among the substrates. Palm Chaff had the highest ash content (7.14%), followed by Oil Palm Empty Bunch (5.08%), Yam Peel (4.29%), and Saw Dust (1.36%). This indicated that Palm Chaff was the richest in minerals, while sawdust had the lowest mineral content. In addition, Yam Peel had the highest moisture content (8.46%), followed by Palm Chaff (5.87%), Oil Palm Empty Bunch (4.62%), and Saw Dust (2.83%). This suggested that Saw Dust was the driest substrate. Furthermore, Oil Palm Empty Bunch had the highest crude lipid content (1.94%), followed by Palm Chaff (1.38%), Yam Peel (1.13%), and Saw Dust (0.04%). Moreover, Oil Palm Empty Bunch had the highest crude protein content (2.56%), followed by Yam Peel (2.1%) and Palm Chaff (2.02%). Saw Dust had the lowest crude protein content (1.74%). Besides, Oil Palm Empty Bunch had the highest crude fiber content (48.27%), followed by Palm Chaff (36.94%), Saw Dust (26.17%), and Yam Peel (14.36%). In addition, Saw Dust had the highest carbohydrate content (67.86%), followed by Yam Peel (69.64%) and Palm Chaff (46.65%). Oil Palm Empty Bunch had the lowest carbohydrate content (37.58%). These findings offered valuable insights into the suitability of each substrate for cultivating Pleurotus ostreatus Additionally, it enabled growers to make informed decisions about substrate selection based on the specific nutritional needs and growth requirements of the mushrooms. The impact of various organic waste substrates on the growth dynamics of Pleurotus ostreatus (oyster mushroom), revealed significant variations in several key parameters. In primordial Formation, the number of early-stage fruiting bodies formed varied between 12 and 36 across different substrate formulations. The highest primordial formation was observed in treatment A + B + C + D (36), while treatments involving individual substrates or their combinations had the lowest primordial formation (12). Furthermore, in Fruiting Body Production, the number of mature mushrooms produced varied across treatments. Treatment A + C resulted in the highest number of fruiting bodies (26), while treatments involving combinations of substrates (B + C + D, A + B + C, A + B + C + D, C + D) had the lowest number of fruiting bodies (3). Overall, the findings highlight the influence of specific substrate combinations on various aspects of mushroom growth dynamics. However, the choice of growth substrates did not significantly impact the overall weight and biological efficiency of Pleurotus ostreatus in this study. These results provide valuable insights for optimizing mushroom cultivation practices.

The influence of dietary supplementation with oyster mushroom waste on laying hens’ performance, egg quality and immune parameters

The objective of the present study was to evaluate the effects of dietary supplementation with oyster mushroom (Pleurotus ostreatus) waste (OMW) on performance, egg quality, fatty acid (FA) profile and oxidative stability, serum and yolk cholesterol and immune parameters of laying hens. Two hundred fifty-six laying hens were allocated into 4 treatment groups, with eight replicate cages, and were fed for 28 d either a control diet, or diets supplemented with OMW at 1, 2 or 4 g per 100 g feed (P1, P2 and P4 experimental groups, respectively). No significant effects were detected on the performance and egg quality (P > 0.05) except from a modest decrease in the intensity of orange yolk color from hens fed with OMW, as indicated by the reduced DSM YolkFan score and color parameter a* (redness) (P < 0.05), and the tendency for increased color parameter L* (lightness) (P < 0.1). Serum total cholesterol and high-density lipoprotein content were decreased in OMW experimental groups compared with the control group (P, P-linear < 0.05) whereas yolk cholesterol content was unaffected (P > 0.05). The ratio of heterophils to lymphocytes were not influenced by dietary treatment (P > 0.05), whereas T helper lymphocyte (Th) percentage was increased in OMW supplemented groups in comparison with control (P < 0.05, P-quadratic < 0.05). The yolk FA profile was beneficially affected, as shown by the linear increase in polyunsaturated FA and a linear decrease in saturated FA in OMW supplemented groups of hens (P-linear < 0.05), with most notable effects observed in the P4 group. Furthermore, oxidative stability, that was expressed as malondialdehyde content, of both fresh and stored egg yolks for up to 90 d, was significantly improved in OMW supplemented groups (P < 0.05). The beneficial effects of OMW on yolk oxidative stability and FA profile, without adverse effects on performance or egg quality, shows that this byproduct may be successfully employed in laying hens' diets, in a circular economy scheme, with benefits not only for the consumers and farmers but for the environment as well.

Development of food for special dietary uses of diabetes based on oyster mushroom and brown rice

Diabetes patients often struggle to meet their energy requirements, prompting the suggestion of utilizing Food for Special Dietary Uses (FSDU). Previous research has shown the efficacy of oyster mushrooms’ B-glucan in controlling hyperglycemia and insulin resistance. Brown rice, high in magnesium and fiber with a low glycemic index, is known to lower blood glucose levels. Utilizing both ingredients in FSDU may provide products with recommended energy value, nutrient ingredients, and glycemic index. This study aimed to measure the energy, protein, fat, carbohydrate, fiber, glucose, and glycemic index of four mixed formulations of oyster mushrooms and brown rice, along with a control.The study used a true experimental design with a completely randomized research design. The formulations included: P1=9% mixture of moringa and fish flour, P2=10% mixture of carrot and fish flour, F3=11% mixture of moringa and tempeh flour, and F4=12% tempeh flour. The total energy was measured using various methods: adiabatic oxygen bomb calorimeter for energy, Kjeldahl for protein, Soxhlet for fat, by-difference for carbohydrates, AOAC enzymatic-gravimetric for total dietary fiber, anthrone for total sugar, and blood glucose tests at 0, 15, 30, 45, 60, 90, and 120 minutes post-test-food ingestion for glycemic index measurements. Statistical analysis was performed using the One-Way ANOVA and Kruskal-Wallis tests.The research results indicate significant differences in energy, protein, fat, carbohydrates, total sugar, and fiber values among groups (p&lt;0.01). The lowest glycemic index was found in P4 (44.32, medium category). Further analysis indicated that P4 had higher total energy and fat, but lower carbohydrates, total sugar, and glycemic index. According to the Multiple Attribute Zeleny Method, the P4 formula was the best formula for all parameters.The most effective formula, containing brown rice, oyster mushrooms, and tempeh flour (P4), could be a beneficial option for improving the health of individuals with diabetes in the community.

Transcriptome Analysis Reveals the Effect of Oyster Mushroom Spherical Virus Infection in Pleurotus ostreatus.

Oyster mushroom spherical virus (OMSV) is a mycovirus that inhibits mycelial growth, induces malformation symptoms, and decreases the yield of fruiting bodies in Pleurotus ostreatus. However, the pathogenic mechanism of OMSV infection in P. ostreatus is poorly understood. In this study, RNA sequencing (RNA-seq) was conducted, identifying 354 differentially expressed genes (DEGs) in the mycelium of P. ostreatus during OMSV infection. Verifying the RNA-seq data through quantitative real-time polymerase chain reaction on 15 DEGs confirmed the consistency of gene expression trends. Both Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses highlighted the pivotal role of primary metabolic pathways in OMSV infection. Additionally, significant changes were noted in the gene expression levels of carbohydrate-active enzymes (CAZymes), which are crucial for providing the carbohydrates needed for fungal growth, development, and reproduction by degrading renewable lignocellulose. The activities of carboxymethyl cellulase, laccase, and amylase decreased, whereas chitinase activity increased, suggesting a potential mechanism by which OMSV influenced mycelial growth through modulating CAZyme activities. Therefore, this study provided insights into the pathogenic mechanisms triggered by OMSV in P. ostreatus.

Antimicrobial properties of the edible pink oyster mushroom, Pleurotus eous: In-vivo and in-vitro studies

In recent times, there has been a notable surge in the investigation of new antibiotic substances derived from natural origins. Pleurotus eous is an edible mushroom that has various useful bioactive substances and therapeutic properties, including antimicrobial activity. The present study aims to evaluate the antimicrobial efficacy of the methanolic extract of P. eous (MEPE) through in vitro method. Notably, S. aureus demonstrated the highest susceptibility to MEPE, prompting further investigation into its antibacterial mechanisms via scanning electron microscopy (SEM), membrane integrity, and permeability assays. The in-vivo antibacterial effect of MEPE against S. aureus was also assessed, including analysis of bacterial burden in organs, hematological profiles, and cytokine profiles. Detailed phytochemical analyses of MEPE were conducted using GC-MS. Results revealed MEPE's significant (p < 0.05) efficacy against Gram-positive bacteria, particularly S. aureus (77.56 ± 0.4 μg/mL and 34 ± 6.9 μg/ml in turbidometric and viable cell count assays, respectively). Moreover, membrane permeability significantly increased in 60.32 % of S. aureus isolates following treatment with MEPE. Additionally, mice receiving MEPE exhibited decreased levels of TNF-α, IL-1β, and IL-6, suggesting its potential in combating S. aureus infection in animal models.

Optimalisasi Waktu Produksi Jamur Tiram Melalui Implementasi Sistem Penyiraman Berbasis IoT di UD Zaida Jamur Tiram

The optimization of oyster mushroom production time through the implementation of an Internet of Things (IoT)-based irrigation system at UD Zaida Jamur Tiram aims to enhance efficiency and production quality. This program integrates IoT technology to automate the irrigation process, allowing real-time control and monitoring of environmental conditions. Methods include the design and installation of an automatic irrigation system, technical training for operators, and performance evaluation through production data analysis. Results show a significant increase in time efficiency and consistency in oyster mushroom quality. The implementation of this IoT system is expected to support the sustainability and competitiveness of the oyster mushroom industry at UD Zaida.