Platelet-rich Fibrin Matrix Research Articles

Efficacy of Platelet-Rich Plasma in Reduction of Post-Operative Split-Thickness Skin Graft Loss and Hematoma Formation: A Meta-Analysis.

Split-thickness skin grafting is a very popular technique of wound closure, especially for large wounds. The success rate of a split-thickness skin graft (STSG) has consistently been in the range of 70-90%. Multiple techniques have been introduced to further improve graft survival, for example, the use of autologous platelet-rich plasma (PRP), thrombin gel, platelet-rich fibrin matrix, and negative pressure wound therapy. We evaluated the impact of PRP use on the survival of STSG through a meta-analysis. We conducted the analysis in accordance with Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) protocol and performed a literature search using the following databases: PubMed, Cochrane, and ClinicalTrials.gov. A total of 126 articles were yielded by the search, out of which four randomized controlled trials (RCTs) were included according to our eligibility criteria and irrelevant articles were excluded. The intervention group received PRP application to the wound bed before applying the graft while the control group received treatment with conventional fixation procedures (sutures and staples). We estimated the pooled odds ratio with a 95% confidence interval (CI). The total number of participants in the analysis was 460. The participants had wounds of varying etiology. Post-operative graft loss and hematoma formation were taken to be the primary and secondary outcome measures, respectively. Thirty-four participants suffered graft loss of differing extent in the control group while three participants suffered graft loss in the intervention group. The pooled odds ratio for graft loss was 0.15 (95% CI: 0.05-0.49; I2=38%; p=0.184) signifying that PRP use decreased the odds of graft loss by 85%. For our secondary outcome measure, 440 participants were studied. Forty-four participants suffered hematoma formation in the control group versus 11 in the intervention group. The pooled odds ratio for hematoma formation was calculated as 0.21 (95% CI: 0.09-0.50; I2=0%; p=0.869) signifying that PRP use decreased the odds of hematoma formation by 79%. PRP appears to significantly impact graft survival, and further studies are needed to strengthen the evidence base for its use in split-thickness skin grafting.

Association between platelet-rich fibrin and collagen matrix for root coverage: Case Series.

Association between platelet-rich fibrin and collagen matrix for root coverage: Case Series.

Management of external root resorption with Biodentine and platelet-rich fibrin matrix: A case report with 3 year follow up

A male patient aged 28 years presented with a chief complaint of discolored left central incisor and missing tooth, due to road traffic accident 15 years ago with no associated symptoms. Clinical and radiographic examination revealed no pulp to response to vitality test, large periapical radiolucency, and root resorption of the middle third of the root. A definite diagnosis of pulpal necrosis with asymptomatic apical periodontitis and external resorption was made. Root canal treatment was done, and the extent of the lesion was analyzed and planned for surgical management. The periapical lesion was curetted, and the external resorption site was sealed with a matrix of Biodentine and platelet-rich fibrin. The case has shown successful healing after 3-year follow-up.

A Comparative Study on Therapeutic Efficacy of Autologous Platelet Rich Fibrin Matrix Versus Zinc Oxide And Phenytoin Paste in Non Healing Ulcers.

Context:A non-healing ulcer occurs due to multiple causes and possesses a great impact on the quality of life. Autologous platelet-rich fibrin and the application of a triple combination paste (zinc oxide, phenytoin, and mupirocin ointment) have emerged with a good response in the treatment of non-healing ulcers.Aims:To compare the therapeutic efficacy of autologous platelet-rich fibrin matrix versus the triple combination paste (zinc oxide, phenytoin, and mupirocin ointment) in non-healing ulcers.Settings and Design:Hospital-based interventional comparative study.Materials and Method:Twenty-four patients with non-healing ulcers were randomly divided into two groups. One group received platelet-rich fibrin (PRF) therapy every week for up to 5 weeks and the second group applied triple combination paste (zinc oxide, phenytoin, and mupirocin ointment) again for 5 weeks. In the end, the ulcer area was measured.Statistical Analysis Used:Student's paired t-test was used to evaluate the correlations between variables. A P-value of < 0.05 was considered significant.Results:Group A showed a mean reduction in the ulcer area by 8.26 mm2 (75.99%). Group B showed a mean reduction in the ulcer area by 4.799 mm2 (47.75%), which was statistically significant with a P-value of 0.004.Conclusion:We conclude that autologous platelet-rich fibrin matrix is much more effective than the triple combination paste (zinc oxide, phenytoin, and mupirocin ointment) in the treatment of non-healing ulcers.

Effect of platelet-rich fibrin matrix in complex with artificial material Nubiplant on expression of chondrogenic marker genes and morphogenesis of the nucleus pulposus cells of intervertebral discs in rats

The purpose was to study the barrier and biological properties of platelet-rich fibrin matrix (PRFM), an artificial biopolymer Nubiplant, and a mixture of PRFM / Nubiplant by assessing the viability and morphological characteristics of nucleus pulposus (NP) cells in rats, as well as the expression level of chondrogenic marker genes during cell cultivation in the presence of these matrices.Materials and methods. PRFM was obtained from platelet-rich plasma using a SiO2 coagulation activator. A suspension of nucleus pulposus cells was obtained from the caudal spine of rats. Cultivation was carried out in the presence of one of three matrices — PRFM, Nubiplant, or their mixture for 3, 7, and 14 days under standard culture conditions in an EC-160 incubator (Nüve, Turkey). Observation of the living culture was carried out in the area bordering with the matrix within one field of view using an inverted microscope (Nicon TS100, Japan). The expression of chondrogenic marker genes in the cell culture of the NP was determined by the method of PCR with reverse transcription.Results. The study of the viability and morphological characteristics of NP cells during their cultivation for 3, 7, and 14 days in the presence of PRFM, PRFM / Nubiplant, or Nubiplant showed a decrease in the content of living cells in control samples; in cultures with PRFM and PRFM / Nubiplant, the number of living cells significantly exceeded the control values, aggregation of cells was observed in the area bordering with the matrices from the side of the application. None of the experimental samples showed the outflow of cells to the opposite side of the matrix after 14 days of cultivation; thus, PRFM, Nubiplant, and their mixture can perform barrier functions to keep the cell population in a certain location. Expression of the COL II, ACAN, GPC3, ANXA3, PTN, MGP, and VIM genes by the NP cells during cultivation for 3 and 7 days in the presence of PRFM and PRFM / Nubiplant increased as compared to the control samples.Conclusions. The use of PRFM, Nubiplant, or a mixture of PRFM / Nubiplant during the cultivation of NP cells demonstrated the absence of cell outflow to the opposite side of the studied matrices during the study period (14 days). The use of PRFM, Nubiplant, or a mixture of PRFM / Nubiplant promoted the formation of cell colonies with chondrocyte-like morphology in the zone bordering with the matrices and maintained cell viability throughout the study period. PRFM and PRFM / Nubiplant contributed to the maintenance of the expression of chondrogenic genes in the NP cells in the zone bordering the matrices. The results obtained indicate the positive effect of the matrix based on platelet-rich fibrin on the NP cells and its barrier functions, which is promising for the use of PRMF for preventing the formation of cicatricial adhesion.

Use of platelet-rich plasma in the treatment of medication-related osteonecrosis of the jaw. A case report

Introduction : Medication-related osteonecrosis of the jaw (MRONJ) is an area of uncovered bone in the maxillofacial region that does not heal within 8 weeks after identification by a healthcare provider, in a patient who is receiving or has been exposed to bisphosphonate therapy without previous radiation therapy to the craniofacial region. Case Description : The survey was conducted in 2018/2019. It was conducted at the Clinic of Maxillofacial Surgery, St. George University Hospital, Plovdiv, Bulgaria. In order to conduct the study, we obtained informed consent from the patient, who was 63 years of age. We have diagnosed medication-related osteonecrosis of the lower jaw. We performed bone resection and applied platelet-rich plasma (PRF) in order to enhance soft tissue healing. Discussion : Management of MRONJ remains a challenge and controversial topic. Multiple treatment approaches have been introduced to control MRONJ. Surgical treatment remains one of the most reliable methods but it also needs to take advantages of advanced scientific researches and technologies. One of these achievements is the use of a platelet-rich fibrin matrix (PRFM). Conclusion: In addition to the established surgical treatment options, we can add the application of platelet-rich plasma as an adjuvant treatment for MRONJ.

Platelet Rich Fibrin Augmented Versus Non-Augmented Glycerolized Bovine Pericardium and Polypropylene Mesh for Repairing of Large Abdominal Wall Defects

This study aimed to evaluate the use of glycerolized bovine pericardium (GBP) compared to polypropylene mesh (PPM) in repairing of large abdominal wall defects in animal model, and to investigate the role of platelet rich fibrin (PRF) in promoting this repair. Fresh bovine pericardium collected from local abattoir were processed and preserved in 99.5 percent glycerol. PRF matrix was harvested from fresh autologous blood (10 ml) after centrifugation. Full-thickness, mid-ventral abdominal wall defects (6 × 10 cm) were surgically created in 36 healthy goats (9 goats/group) and were repaired with an equal size of GBP, PPM, GBP-PRF, or PPM-PRF. Qualitative and gray scale quantitative ultrasonography were adopted at day 1, 1, 2, 3, 4, 8 and 12 weeks post-implantation. Three goats per group were slaughtered at 4, 8 and 12 weeks post-implantation for further gross, histopathological and tensiometric (tensile strength, load at failure and strain percent) evaluations. Ultrasonography revealed significant (P˂ 0.05) improvement of implant gray scale, low subcutaneous edema and reduction of skin implant distance in PRF-augmented groups. Besides, a substantial improvement of connective tissue covering, implant incorporation, new blood vessels formation, and reduction of the inflammatory cells infiltrations were observed. Tensiometric parameters were improved in GBP-PRF group compared to the other groups. In conclusion, the obtained results not only proved the superiority of GBP over PPM, but also the advantage of PRF-augmented over non-augmented implants in treatment of large abdominal wall defects. Ultrasonographic analysis provided a satisfactory tool to evaluate the healing process of the abdominal wall defects.

INCUBATION OF PLATELET-RICH FIBRIN MATRIX WITH MESENCHYMAL STEM CELLS IMPROVES MATRIX STIFFNESS

Objective: The platelet-rich fibrin matrix (PRFM) is condensed platelet-rich plasma (PRP) and should possess a comparable biomechanical property to the transplanted sites, for them to be physiologically functional. The aim of this study was to investigate the effect of human bone marrow mesenchymal stem cells (hBM-MSC) or human umbilical cord mesenchymal stem cells (hUC-MSC) on the biomechanical properties of PRFM.&#x0D; Methods: PRFM was prepared by the gelation of PRP using 25 mmol CaCl2. The resulting coin-shaped PRFM pellets, 5 cm in diameter and 300 μm thick, were directly seeded with hUC-MSC or hBM-MSC at 2,000 cells cm-2, followed by 24 h incubation at 37 °C in 5% (v/v) CO2 in air. The samples were then observed by scanning electron microscopy to determine the morphology of the matrix surface. The PRFM biomechanical properties were determined at a 10 mm. min-1failure rate using an MCT 2150 universal testing machine (AandD Co. LTD).&#x0D; Results: SEM imaging of the surface of the PRFM seeded with hBM-MSC and hUC-MSC showed a cloudy layer that thickened over time. The elastin slope of the PRFM was significantly improved after seeding with hBM-MSC and hUC-MSC when compared with unseeded PRFM (p&lt;0.002, R2=0.983). Both cell types elicited similar biomechanical effects (p=0.99).&#x0D; Conclusion: PRFM seeded with hBM-MSC or hUC-MSC showed significantly increased elasticity.

The Effect of Platelet-Rich Fibrin Matrix on Skin Rejuvenation: A Split-Face Comparison.

Despite the increasingly popular use of platelet-rich fibrin matrix (PRFM) for facial rejuvenation, no studies have evaluated its effects on skin. The aim of this study was to determine the effect of PRFM on skin quality as measured by the Canfield VISIA Complexion Analysis System. This was a 12-week randomized, placebo-controlled trial conducted at a single center comparing 2 groups. Thirty patients received a PRFM injection in the mid-cheek region and nasolabial fold on one side of the face and saline on the contralateral side. The primary outcome measure was the difference between pre- and posttreatment total VISIA skin scores for each group at 6 and 12 weeks. The change in subscores for each skin parameter was also calculated. There were 30 participants, with a mean [standard deviation] age of 49.9 [13.9] years. At 6 weeks, the median change in total VISIA score (interquartile range) was -1.77 (2.36) in the PRFM group and -0.73 (2.09) in the saline group (P = 0.003). The only skin parameter that had a significantly different change in score between the groups was texture (P = 0.004). At 12 weeks, the change in median score was -1.31 (3.26) in the PRFM cohort and -0.76 (2.21) in the saline cohort (P = 0.34). There was no statistical significance in the change in score for any of the individual skin parameters. PRFM can objectively improve skin quality compared with placebo. Texture was the only skin parameter that significantly improved, which is consistent with PRFM's role as a filler agent. The results appear to persist for at least 6 weeks.

The use of piezoelectric instrumentation and platlet rich fibrin matrix in septorhinoplasty: Report of two cases

Introduction. Rhinoplasty is one of the most commonly performed surgeries in the area of aesthetic surgery. Surgical instruments, which are used in traditional rhinoplasty, like saws, chisels and osteotomes are relatively imprecise and their usage can lead to uncontrolled fractures of the bone and consequently to inadequate final results. Piezoelectric-powered ultrasonic instruments (PEI) are currently the most innovative instrumentation available for minimally traumatic reshaping of the bony vault and lateral walls. There are many studies which have shown positive effects of platelet-rich fibrin (PRF) in postoperative course of rhinoplasty patients. Case report. We presented two innovative approaches in rhinoplasty combined PEI and PRF matrix through two case reports. In both patients, satisfying results were achieved by use of PEI technique. Also, usage of PRF membrane provided good healing and small postoperative edema. Conclusion. Based on our experience, the use of of PEI technique has many benefits. It is safe, practical and effective method and it demonstrates valuable and favourable results in osteotomies. Also, usage of PRF membrane helps patients in better healing and less postoperative edema.

Clinical Assessment of Platelet Rich Fibrin Matrix in Treatment of Gingival Recession: A Randomized Controlled Clinical Study

Purpose: to measure platelet rich fibrin matrix PRFM in enhancing root coverage of Miller class I and II gingival recession clinically by coronally advanced flap. Materials and Methods: Eighteen healthy patients were randomly splitted for three groups: group I (Test group): five patients treated using Platelet Rich Fibrin Matrix (PRFM) membrane and coronally advanced flap, group II (Positive Control group): fivepatients treated by collagen membrane and coronally advanced flapand group III (Negative control group):five patients treated only using coronally advanced flap. Recession width(RW), recession depth(RD), and probing pocket depth (PPD)were evaluated at baseline,three months and six months respectively. Results: In comparing all groups at all time intervals recorded in RD and RW, a statistically significant difference was found.A great reduction in PPD were recorded at 6 monthsthan at 3 month (P-value = 0.50). However, this different was statistically insignificant. Conclusion: The use of PRFM was effective in treatment of gingival recessions. However, there was no difference recorded between the use of a coronally advanced flap alone and a coronally advanced flap combined with PRFM or collagen membrane in root coverageafter 6 months.

Quantitative Near-Infrared Imaging of Platelets in Platelet-Rich Fibrin (PRF) Matrices: Comparative Analysis of Bio-PRF, Leukocyte-Rich PRF, Advanced-PRF and Concentrated Growth Factors.

Platelet-rich fibrin (PRF) is a fibrin matrix enriched with platelets. The PRF matrix is thought to form a steep gradient of platelet density around the region corresponding to the buffy coat in anticoagulated blood samples. However, this phenomenon has not yet been proven. To visualize platelet distribution in PRF in a non-invasive manner, we utilized near-infrared (NIR) imaging technology. In this study, four types of PRF matrices, bio-PRF, advanced-PRF (A-PRF), leukocyte-rich PRF (L-PRF), and concentrated growth factors (CGF) were compared. Blood samples collected from healthy, non-smoking volunteers were immediately centrifuged using four different protocols in glass tubes. The fixed PRF matrices were sagittally divided into two equal parts, and subjected to modified immunohistochemical examination. After probing with NIR dye-conjugated secondary antibody, the CD41+ platelets were visualized using an NIR imager. In L-PRF and CGF, platelets were distributed mainly on and below the distal surface, while in bio-PRF and A-PRF, platelet distribution was widespread and homogenous. Among three regions of the PRF matrices (upper, middle, and lower), no significant differences were observed. These findings suggest that platelets aggregate on polymerizing fibrin fibers and float up as a PRF matrix into the plasma fraction, amending the current “gradient” theory of platelet distribution.

Effect of platelet-rich plasma and platelet-rich fibrin matrix on healing of vertical meniscal tears in a rabbit model.

This study aimed to investigate the effect of platelet-rich plasma (PRP) and platelet-rich fibrin matrix (PRFM) on the healing of vertical medial meniscal tears in a rabbit model. The study was conducted on 72 New Zealand mature rabbits aged more than 6 months. Rabbits were randomly assigned to six groups: control (C) group, meniscal repair (MR) group, PRP group, PRFM group, MR+PRP group, and MR+PRFM group, with 12 rabbits in each group. A 5-mm full-thickness vertical tear was created in the avascular zone of the medial meniscus corpus in the right knee of all rabbits. The respective treatment for each group was given to the meniscal tear in each rabbit. Histological evaluation of healing was performed 6 and 12 weeks after surgery. Defect filling and collagen formation remained low in the C group. However, in all other groups, there was no significant difference in the 6th week. In the 12th week, similar results as those obtained in the 6th week were obtained. In the C group, there was a difference in defect filling and cell type. This difference was that the defect filling and collagen formation remained low in the 12th week. No significant difference was observed between MR, MR + PRP and MR + PRFM groups. The MR group significantly differed from the other groups in the defect-filling rate and cell type; however, the use of PRP and PRFM did not provide an obvious benefit. The contradictory results obtained in previous studies emphasize the need for further research on the use of PRP in meniscal recovery and repair. We believe that if surgery is indicated, repair is absolutely necessary to improve the healing of the tissue in meniscal tears. Studies using human meniscal tissue for meniscal injury and those that evaluate clinical applications of PRP are warranted.

Platelet-rich plasma, platelet-rich fibrin, and enamel matrix derivative for oral mucosal wound healing.

Different approaches to enhance healing of hard or soft tissues include the use of cytokines and growth factors to modify cellular behaviour. Numerous growth factors are found in autologous blood concentrates - platelet-rich plasma (PRP) and platelet-rich fibrin (PRF). Enamel matrix derivative (EMD) may improve tissue healing via amelogenins. Bilayered collagen matrix (CM) is used for soft tissue augmentation. The aim of the present study was to assess potential benefits of PRP, PRF and EMD in combination with bilayered collagen matrix or CM alone in treatment of oral mucosal defects in rabbits. Twenty-seven New Zealand white rabbits were included in this randomized controlled trial. Artificial oral mucosal defects were treated with one of these five approaches: PRP+CM, PRF+CM, EMD+CM, CM alone, or left untreated as a negative control - CO. The animals were euthanized 1 day, 7 days, or 28 days after surgery and necropsies were harvested. Histological and molecular biological analyses were performed. All defects were healed by day 28. No differences between PRP+CM, PRF+CM, CM alone and CO groups were recorded at any time point. Slower angiogenesis and a higher presence of inflammatory infiltrate were observed in the EMD+CM group 28 days after surgery. Molecular biological analyses did not reveal any statistically significant changes. In conclusion, no improvement in mucosal healing of wounds covered with a collagen membrane and PRP, PRF, or EMD was observed, compared with CM alone or untreated controls.

Acute cytotoxic effects of silica microparticles used for coating of plastic blood-collection tubes on human periosteal cells

Because of its simple operation, platelet-rich fibrin (PRF) is becoming more popular than the original form, platelet-rich plasma (PRP), in regenerative dentistry. PRF preparation requires plain glass blood-collection tubes, but not either anticoagulants or coagulation factors. However, such glass tubes designed for laboratory testing are no longer commercially available. Although several glass tubes specifically designed for PRF preparation are available, many clinicians prefer to obtain stably supplied substitutes, such as silica-coated plastic tubes produced by major medical device companies. The quality of PRF prepared by silica-coated tubes has not been assessed and we previously reported significant contamination of silica microparticles in the resulting PRF matrix and alerted clinicians against the use for PRF preparation. To further assess the biosafety of the silica microparticles, we presently examined their effects on human normal periosteal cells derived from alveolar bone. The periosteal cells were obtained from explant cultures of small periosteal tissues obtained from healthy donors. Silica microparticles were obtained from silica-coated tubes and added to cell cultures. Cellular responses were monitored using a tetrazolium assay, phase-contract inverted microscopy, an immunofluorescence method, and scanning electron microscopy. Silica microparticles adsorbed onto the cell surface with seemingly high affinity and induced apoptosis, resulting in significant reduction of cell proliferation and viability. These findings suggest that silica microparticles contained in plastic tubes for the purpose of blood coagulation are hazardous for various cell types around sites where silica-contaminated PRF matrices are implanted.

Evaluation of vascular endothelial growth factor - A release from platelet-rich fibrin, platelet-rich fibrin matrix, and dental pulp at different time intervals.

Background:Lack of collateral blood supply and the low compliance environment of pulp makes its healing, a challenge. Vascular endothelial growth factor (VEGF) is a primary angiogenic growth factor. Knowledge of dose and time-dependent expression of VEGF from platelet concentrates, namely platelet-rich fibrin (PRF) and PRF matrix (PRFM), along with vital pulp can aid in developing strategies to improve the outcome of vital pulp therapy and regenerative procedures. Hence, the aim of this study was to compare VEGF kinetics of PRF, PRFM, and dental pulp.Materials and Methods:The PRF, PRFM, and vital dental pulp were placed in culture media for a week; the supernatant was collected from these samples at days 1, 4, and 7. VEGF-A expression was evaluated using ELISA and compared with the weight of the sample so as to quantify the release of VEGF-A per milligram of sample.Results:PRF exhibited maximum VEGF-A release on day 4 and was sustained till day 7. In contrast, PRFM and dental pulp showed no significant release of VEGF-A till day 7. However, on day 7, there was a rapid increase in VEGF-A expression from dental pulp that was comparable to PRF. On comparing the release of VEGF-A per milligram of tissue, pulp exhibited the maximum values.Conclusion:Among the platelet concentrates, differential expression of VEGF-A was superior in PRF. The use of PRF in partial pulpitis should be explored in order to restore pulp vascularity and hasten pulpal healing.

Platelet-rich fibrin and collagen matrix for the regeneration of infected necrotic immature teeth

This article documents the 2-year outcome of regenerative endodontic treatment in necrotic immature teeth. The successful outcome of this case shows that regenerative endodontic teeth can be a viable treatment option in immature teeth.

To Evaluate the Efficacy and Safety of Autologous Platelet Rich Fibrin Matrix in the Treatment of Non-Healing Ulcer of Various Aetiology

To Evaluate the Efficacy and Safety of Autologous Platelet Rich Fibrin Matrix in the Treatment of Non-Healing Ulcer of Various Aetiology

The Effect of Platelet-Rich Fibrin (PRF), Plasma Rich in Growth Factors (PRGF), and Enamel Matrix Proteins (Emdogain) on Migration of Human Gingival Fibroblasts.

Statement of the Problem: Platelet preparations such as plasma rich in growth factors (PRGF), platelet-rich fibrin (PRF), or enamel matrix proteins (Emdogain) are commonly used for soft and hard tissue regeneration. However, their comparative effectiveness has not been extensively studied, and a consensus has yet to be reached on their efficacy. Purpose:The aim of this in vitro study was to compare the effect of PRF, PRGF, and Emdogain on the proliferation of human gingival fibroblasts (HGF). Materials and Method: Artificial wounds were made in HGF cell culture after the fibroblasts reached nearly 100% of confluence. The study groups included leukocyte and platelet-rich fibrin (LPRF), pure platelet-rich fibrin (PPRF), PRGF and Emdogain. Stained cells were photographed at 48 h and one week and the percent of wound filling was measured. Statistical analysis was performed by one-way ANOVA and p values < 0.05 were considered statistically significant. Results:The highest wound filling percentages at both time intervals were observed in the PPRF group, followed by the PRGF. The lowest percentage of wound healing among test groups was observed in the LPRF while Emdogain yielded modest results. However, statistical analysis showed similar wound healing values in PRGF, PPRF, and Emdogain groups. Conclusion: Within the limitations of this study, PRGF, PPRF, and Emdogain were similarly effective in enhancing the fibroblast proliferation and artificial wound closure.

Efficacy of autologous platelet rich fibrin matrix in the management of non-healing ulcers

&lt;p class="abstract"&gt;&lt;strong&gt;Background:&lt;/strong&gt; Non healing ulcers have a worldwide prevalence of 1.9%-13.1% with lower extremity being the commonest site. They are difficult to manage for the physician and frustrating for the patient due longer duration of treatment, cost, unsatisfactory outcomes and impairment caused due to them. Standard conventional management may fail at times and hence growth factors derived from platelets have been tried in the management of these ulcers. The purpose of our study was to evaluate the efficacy of platelet rich fibrin matrix (PRFM) in treatment of these ulcers.&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Methods:&lt;/strong&gt; Twelve patients with fifteen non-healing ulcers more than 3 months duration were included in the study. 10cc of venous blood was withdrawn in plain tube without anti-coagulants and centrifuged at 1500 rpm for 14 minutes as early as possible as per advanced PRF protocol. Middle layer of PRFM was applied to the clean ulcer followed by a secondary dressing. All patients received PRFM sittings every 7-10 days or till ulcer healed. Baseline photographs and measurements of length, breadth and depth were taken to calculate the area of ulcer at every sitting.&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Results:&lt;/strong&gt; The average percentage reduction in area and volume of the ulcer was 95.84% and 98.18% respectively at the end of six sittings. Twelve out of fifteen ulcers showed complete healing by 6 weeks, while three ulcers showed significant improvement but did not heal completely. The procedure was safe, well tolerated without any side effects.&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; Platelet rich fibrin matrix is a novel modality and an ideal, safe, affordable therapeutic option for non-healing wounds of varied causes.&lt;/p&gt;