Addition Of Plasticizer Research Articles

Recycling of Post-Consumer Waste Polystyrene Using Commercial Plastic Additives

Recycling of Post-Consumer Waste Polystyrene Using Commercial Plastic Additives

Disentangling mechanisms by which microplastic films affect plant-soil systems: physical effects of particles can override toxic effects of additives

BackgroundMicroplastics, polymer-based particles < 5 mm, affect plant–soil systems positively or negatively, suggesting there are different modes of action. Microplastics, as particles, have physical effects but the leaching of additives likely contributes chemical mechanisms, both of which may be dependent on microplastic size. To disentangle such mechanisms, we established a controlled experiment involving polypropylene and polyethylene films of small, medium and large size, and we evaluated the individual and combined effect of plastic particles and additives (leachates from plastic particles) on soil properties and plant performance of the phytometer Daucus carota and on bare soils.ResultsWe find that additives better explained variation in soil properties (e.g., 44.6% vs 1.3%). Soil respiration and aggregation were negatively affected for additives, likely due to the presence of toxic substances. Overall, such effects increased as plastic size decreased. By contrast, plastic particles better explained plant biomass responses. The positive effect of particles on aeration which may promote root penetration and nutrient uptake, and microplastics itself as a source of carbon potentially promoting soil microbial activity, help explain the positive effect of particles on plant biomass. Plants mitigated the negative effects of additives on bare soils while enhancing the positive effects of particles. This improvement was likely linked to an increase in root activity and rhizodeposition, as plastic particles improved soil aeration. The combined effect of additives and particles, which mimics the microplastic found in the soil, mitigated their individual negative effects on plant–soil systems. As the negative effect of additives could have been masked by the positive effects of particles, simply reporting net positive effects would capture only part of the response.ConclusionsAdditives and plastic particles differently affect soil properties and plant biomass. Additives primarily negatively affect soil properties due to toxic substances, while plastic particles enhance plant biomass likely by improving soil aeration. When examining microplastics effects on terrestrial systems (i.e., the combined effect of additives and particles), the negative effect of additives may be masked by the positive effects of plastic particles. Reporting only net positive effects risks overlooking these underlying negative effects. Plants can mitigate the negative impacts of additives and amplify the positive effects of plastic particles. Our study emphasizes the importance of investigating both the individual and combined effects of additives and particles to fully understand and address the impacts of microplastics on terrestrial ecosystems.

Characterization of Chemical Exposome in A Paired Human Preconception Pilot Study.

Parental preconception exposure to synthetic chemicals may have critical influences on fertility and reproduction. Here, we present a robust LC-MS/MS method covering up to 95 diverse xenobiotics in human urine, serum, seminal and follicular fluids to support exposome-wide assessment in reproductive health outcomes. Extraction recoveries of validated analytes ranged from 62% to 137% and limits of quantification from 0.01 to 6.0 ng/mL in all biofluids. We applied the validated method to a preconception cohort of Australian couples (n = 30) receiving fertility treatment. In total, 36 and 38 xenobiotics were detected across the paired biofluids of males and females, respectively, including PFAS, parabens, organic UV-filters, plastic additives, antimicrobials, and other industrial chemicals. Results showed 39% of analytes in males and 37% in females were equally detected in paired serum, urine, and reproductive fluids. The first detection of the sunscreen ingredient avobenzone and the industrial chemical 4-nitrophenol in follicular and seminal fluids suggests it can cross both blood-follicle/testis barriers, indicating potential risks for fertility. Further, the blood-follicle transfer of perfluorobutanoic acid, PFOA, PFHxS, PFOS, and oxybenzone corroborate that serum concentrations can be reliable proxies for assessing exposure within the ovarian microenvironment. In conclusion, we observed significant preconception exposure to multiple endocrine disruptors in couples and identified potential xenobiotics relevant to male and female fertility impairments.

Evaluating microplastic particles as vectors of exposure for plastic additive chemicals using a food web model

Microplastic particles (MPs) represent potential hazards for humans and wildlife, including as vectors for chemical exposure (e.g. plastic additives and pollutants sorbed from the surrounding environment). The leaching of chemicals from MPs has been identified as a potential exposure pathway but the relative magnitude of this pathway under environmentally relevant conditions remains unclear. Here, we describe a modification of the ACC-HUMANSTEADY bioaccumulation model to include dietary exposure to MPs containing either accumulated chemicals from the surrounding environment or embedded plastic additive chemicals (PACs). Chemical transfer to humans and wildlife is described using two-film resistance concepts assuming spheroidal particles of different sizes. The relative contribution of MPs and environmental media to the estimated daily chemical intake in humans was assessed in various exposure scenarios, for a range of hypothetical chemicals with varying octanol-water and air-water partition coefficients (KOW and KAW, respectively; i.e. 0 < log KOW <8 and − 5 < log KAW < 3). Results imply that MPs could act as sources of exposure to chemical additives when the ingestion rate of 1 μm MPs is ≥ 10 mg d− 1, and the concentration of hydrophobic plastic additive is ≥ 5% wt wt− 1. The contribution made by MPs as vectors of exposure decreased with increasing particle size and decreasing ingestion rates of MPs. Human health risks were evaluated for four specific PACs to illustrate the application of the model for risk assessment. Risks were negligible when the ingestion rate of MPs was < 100 μg d− 1. Uncertainties are high regarding the characterization and quantification of ingestion of MPs by humans and wildlife, including particle sizes and polymer composition, as well as on the presence of PACs in MPs. These data gaps need to be addressed if the issue of MPs as vectors of chemical exposure is to be fully understood. The work illustrates that mechanistic models, which account for all major exposure pathways, can be used to identify the importance of different exposure pathways, help prioritize research needs and support decision making.

Evaluation of char properties from co-pyrolysis of biomass/plastics: effect of different types of plastics

Co-pyrolysis of biomass/plastics is a viable method to obtain high-quality carbon materials. The synergistic effect in the char production process of co-pyrolysis of biomass/plastics affects the properties of the obtained char. However, the synergistic effect research on the char production process of co-pyrolysis of biomass/plastics is facing challenges owing to highly varying composition of different plastics. In this study, the synergistic effect during co-pyrolysis of bamboo (BM) and plastics (polypropylene (PP), polystyrene (PS), polyethylene terephthalate (PET), polycarbonate (PC), and polybutylene terephthalate (PBT)) was investigated. TG results showed that PP, PS, PET, and PBT promoted the release of biomass volatiles and increased co-pyrolysis char yield. Notably, the O-aromatic structure in PC underwent cleavage and deoxygenation reactions, inhibiting the production of co-pyrolysis char during co-pyrolysis process. Char yield from co-pyrolysis process decreased from 24.80% for bamboo pyrolysis to 15.74% for co-pyrolysis of bamboo/PC. The results of physicochemical tests on char samples indicated that the addition of polyhydrocarbon plastics (PP and PS) increased H/C ratio, O/C ratio, heating value and oxidative reactivity of the char compared to bamboo char due to the vapor deposition of hydrocarbons derived from thermal decomposition of plastics on the biomass char. While, co-pyrolysis of biomass and polyester plastics (PET, PC, PBT) improved the pore structure of char and increased oxygen content.

A multidisciplinary perspective on the role of plastic pollution in the triple planetary crisis

In this perspective paper, we discuss the negative impacts of plastics and associated chemicals on the triple planetary crisis of environmental pollution, climate change and biodiversity loss from a multidisciplinary perspective. Plastics are part of the pollution crisis, threatening ecosystems and human health. They also impact climate change and accelerate biodiversity loss; in this, they aggravate the triple planetary crisis. We analyze the scientific state-of-the-art to identify critical knowledge gaps regarding the life cycle, release, fate, exposure, hazard and governance of plastics and associated chemicals, as well as links to climate change and biodiversity loss. Based on the outcome, we derive key research needs for a comprehensive hazard assessment of plastics and associated chemicals, amongst others, to address the largely missing regulation of plastic additives and in-use plastics. We offer a holistic perspective bridging disciplinary expertise from natural and social sciences to achieve effective plastic governance and risk management of plastics and associated chemicals that protect the Earth, its ecosystems and human health from the plastics crisis.

The Influence of the Type and Concentration of Plasticizer on the Properties of Biopolymer Films based on Wild Flax (Camelina Sativa)

The development of biodegradable packaging materials using naturally occurring, renewable biopolymers has gained attraction due to consumer demand for high-quality products and concerns about environmental waste problems. However, the inferior mechanical properties and low water resistance of packaging materials based on natural polymers pose a significant obstacle to their wider use. One of the ways to improve the properties of biopolymer-based packaging materials is the addition of plasticizers during their synthesis. In this work, the influence of the type and concentration of plasticizer on the properties of new biopolymer films based on wild flax (Camelina sativa) was investigated. Camelina sativa oil cake (CSoC) remains after edible oil cold pressing as a by-product. One of the possibilities for its valorization is the synthesis of biopolymer materials. During the synthesis, different plasticizers - glycerol and polyethylene glycol 400 - were added in different concentrations - 20%-60%. The obtained CSoC-based biopolymer films were analyzed for the following properties: Moisture content, solubility, thickness, tensile strength, elongation at break, and water vapor permeability. The results obtained showed significant differences when different plasticizers were applied at different concentrations. The biopolymer film with optimal properties was obtained by adding glycerol at a concentration of 40%.

Rapid Generation of Microplastics and Plastic-Derived Dissolved Organic Matter from Food Packaging Films under Simulated Aging Conditions.

In this study, we show that low-density polyethylene films, a prevalent choice for food packaging in everyday life, generated high numbers of microplastics (MPs) and hundreds to thousands of plastic-derived dissolved organic matter (DOM) substances under simulated food preparation and storage conditions. Specifically, the plastic film generated 66-2034 MPs/cm2 (size range 10-5000 μm) under simulated aging conditions involving microwave irradiation, heating, steaming, UV irradiation, refrigeration, freezing, and freeze-thaw cycling alongside contact with water, which were 15-453 times that of the control (plastic film immersed in water without aging). We also noticed a substantial release of plastic-derived DOM. Using ultrahigh-resolution mass spectrometry, we identified 321-1414 analytes with molecular weights ranging from 200 to 800 Da, representing plastic-derived DOM containing C, H, and O. The DOM substances included both degradation products of polyethylene (including oxidized forms of oligomers) and toxic plastic additives. Interestingly, although no apparent oxidation was observed for the plastic film under aging conditions, plastic-derived DOM was more oxidized (average O/C increased by 27-46%) following aging with a higher state of carbon saturation and higher polarity. These findings highlight the future need to assess risks associated with MP and DOM release from plastic wraps.

Long-term performance of porous asphalt pavement incorporating recycled plastics

Recycling plastic in asphalt pavement holds potential for environmental and economic benefits. However, its performance after prolonged exposure to heat, oxygen, sunlight, and moisture remains uncertain, casting doubt on its viability. In this study, various types of recycled plastics were incorporated into porous asphalt mixtures and exposed to long-term aging conditions. A total of 360 specimens were tested to evaluate several key properties: moisture susceptibility and rutting resistance; abrasion resistance; and indirect tensile strength and fatigue resistance. The results indicate that porous mixtures with 5 % plastic generally exhibit superior long-term performance compared to non-plastic porous mixtures, except for cracking resistance. Excessive addition of plastic (15 %) in asphalt pavement can lead to significant degradation of long-term performance. A trial section incorporating plastic in porous asphalt pavement exhibited satisfactory performance after a year of construction. These insights are pivotal in shaping policy and directing resources toward sustainable road construction practices.

Biological exposure to microplastics and nanoplastics and plastic additives: impairment of glycolipid metabolism and adverse effects on metabolic diseases.

Microplastics and nanoplastics (M-NPs) are widespread pollutants in the environment, posing growing risks to human health and garnering increasing concern from researchers. Due to their small particle size, ease of adsorption, and resistance to degradation, M-NPs can retain and migrate in the environment for long-term periods. Upon entering organisms, M-NPs have been reported to cause inflammation and oxidative stress and result in abnormalities in glycolipid metabolism. Furthermore, research suggests that exposure to M-NPs may act as a causative agent for metabolic and cardiovascular diseases such as diabetes, obesity, and atherosclerosis. This paper aims to review the consequences of exposure to M-NPs on animal and cellular glycolipid metabolism and discusses the disruption of gut microbial homeostasis and the subsequent emergence of insulin resistance. PPAR signaling pathway activation after exposure to M-NPs was found to lead to increased hepatic fat accumulation and impaired lipid metabolism. Additionally, the paper highlights how M-NPs exacerbate the progression of obesity and diabetes in patients, induce damage to vascular endothelial cells, trigger oxidative stress, and contribute to the development of atherosclerosis. Despite the growing concern, the toxicity and molecular mechanism of M-NPs on glycolipid metabolism remain understudied, and effective methods for removing plastic pollutants deposited in the body are yet to be established. These findings provide valuable insights for future research in this field.

Comprehensive inventory of imports of electrical and electronic equipment and related plastics and POPs plastic additives into Nigeria in the past 32 years (1990–2022)

The global management of electrical and electronic equipment (EEE) and related plastics, and the persistent organic pollutants (POPs) contained in it constitute a global challenge in particular for low-income countries in Africa where monitoring capacity and waste management infrastructure are lacking. While plastic recycling is highlighted as an important part of the solution to plastic pollution, such recycling is threatened by the presence of POPs. Overall, there is a lack of data on POPs in (W)EEE plastics with the exception of PBDEs. The objective of this study was to analyse the overall imports of (W)EEE into Nigeria and estimate the associated amount of plastics and POPs for better planning and management of WEEE plastics. This study assessed all EEE importation categories and compiled the importations of 28 EEE product groups with the highest importation, covering more than 98 % of officially imported EEE based on the United Nations Comtrade Database using harmonized system (HS) codes. The impact factors of POPs and plastic from the UNEP inventory guidance were used for the estimates. Overall, 4568 kilo-tonnes (kt) of EEE were officialy imported between 1990 and 2022 containing 1337 kt of plastic. If considering also the estimated imported WEEE and EEE not covered in the Comtrade statistics, the total imported (W)EEE is estimated to 12,259 kt including 3644 kt of plastics and an estimated 1043 t of hexaBDE/heptaBDE and 8511 t of DecaBDE as major POP additives, and lower levels of 154 t of HBCD, 91 t of SCCPs, 364 t of MCCP, and 146 t of Dechlorane Plus. The study is the first estimate of all major POPs in (W)EEE plastics in a low-income country and provides important information for their management.

Substituting corn starch with wolf's fruit and butterfly lily starches in thermopressed films: Physicochemical, mechanical, and biodegradation properties

The industrial use of corn starch competes with food supplies, encouraging the investigation of native starches as an alternative for its partial replacement. This study aimed to analyze the effects of replacing corn starch (CS) with wolf's fruit (WFS) and butterfly lily (BLS) starches on the physicochemical, mechanical, and biodegradation properties of starch-based films. Plasticized (with glycerin and citric acid) and unplasticized films were prepared with a microwave (10 s) and by thermopressing (1.5 t/120 °C/2 min) and were analyzed for amylose, scanning electron microscopy, X-ray diffraction, and paste properties. Furthermore, the biodegradability of films was tested in two soils over 42 days. Our results show that BLS is not a suitable raw material to replace corn starch. WFS with 27.5 % apparent amylose content and granule size of 12.5 μm produced films with thickness, permeability, tensile strength, and elongation of ~110 μm, ~4.8 g (m.s.Pa)−1, ~2.5 MPa, and ~2.9 %, respectively, similar to CS. The biodegradability of WFS film showed greater resistance (≤61.4 %), increasing with the addition of plasticizers (89–93 % for WFS302) or partial replacement of CS (73–91 % for CSWFS303). These findings indicate that WFS can partially or fully replace CS in thermopressed films.

Fluorescent polymers for environmental monitoring: Targeting pathogens and metal contaminants with naphthalimide derivatives

Monitoring Hg2+ levels in aqueous environments is crucial to assess the potential methylmercury contamination via bacterial conversion, however, existing methods often require extensive sample treatment and expensive equipment. To mitigate this issue, this study examines the synthesis and application of three naphthalimide-based compounds, with significant fluorescent and solvatochromic behavior (C1, C2, and C3). Compounds C1 and C2 demonstrated a strong affinity for Hg2+ metal ions, with C2 showing selectivity and a strong antibacterial profile, particularly against S. aureus (MIC50 (C2) = 0.01 µg/mL). Moreover, these compounds were incorporated into three polymeric matrices, namely polyvinyl chloride (PVC), poly (methyl methacrylate-co-methacrylic acid) (PMMMA), and Starch, allowing for the development of solid-support sensors/surfaces with a strong antibacterial profile, highlighting the inherent dual-functionality of the compounds. Interestingly, the C2-doped Starch biopolymer detected low concentrations of Hg2+ ions, such as 23 nM in tap water (value within the WHO standards for drinking water), through a rapid spectroscopic evaluation without sample treatment. This biopolymer was generated via a sustainable, green-chemistry-oriented, temperature-dependent water/Starch synthetic route, without the addition of plasticizers and any associated ecotoxicity. The study used sustainable methods for environmental monitoring and antibacterial applications, advancing material science to offer effective, accessible, and eco-friendly solutions for detecting and mitigating mercury pollution and bacterial contaminations, enhancing environmental and health safety.

Investigating Exposure and Hazards of Micro- and Nanoplastics During Pregnancy and Early Life (AURORA Project): Protocol for an Interdisciplinary Study.

Micro- and nanoplastics (MNPs) are emerging pollutants of concern with ubiquitous presence in global ecosystems. MNPs pose potential implications for human health; however, the health impacts of MNP exposures are not yet understood. Recent evidence suggests that MNPs can cross the placental barrier, underlying the urgent need to understand their impact on reproductive health and development. The Actionable eUropean ROadmap for early-life health Risk Assessment of micro- and nanoplastics (AURORA) project will investigate MNP exposures and their biological and health effects during pregnancy and early life, which are critical periods due to heightened vulnerability to environmental stressors. The AURORA project will enhance exposure assessment capabilities for measuring MNPs, MNP-associated chemicals, and plastic additives in human tissues, including placenta and blood. In this interdisciplinary project, we will advance methods for in-depth characterization and scalable chemical analytical strategies, enabling high-resolution and large-scale toxicological, exposure assessment, and epidemiological studies. The AURORA project performs observational studies to investigate determinants and health impacts of MNPs by including 800 mother-child pairs from 2 existing birth cohorts and 110 women of reproductive age from a newly established cohort. This will be complemented by toxicological studies using a tiered-testing approach and epidemiological investigations to evaluate associations between maternal and prenatal MNP exposures and health perturbations, such as placental function, immune-inflammatory responses, oxidative stress, accelerated aging, endocrine disruption, and child growth and development. The ultimate goal of the AURORA project is to create an MNP risk assessment framework and identify the remaining knowledge gaps and priorities needed to comprehensively assess the impact of MNPs on early-life health. In the first 3 years of this 5-year project (2021-2026), progress was made toward all objectives. This includes completion of recruitment and data collection for new and existing cohorts, development of analytical methodological protocols, and initiation of the toxicological tiered assessments. As of September 2024, data analysis is ongoing and results are expected to be published starting in 2025. As plastic pollution increases globally, it is imperative to understand the impact of MNPs on human health, particularly during vulnerable developmental stages such as early life. The contributions of the AURORA project will inform future risk assessment. DERR1-10.2196/63176.

Producción de esterasa por Fusarium culmorum crecido en presencia de diferentes concentraciones di(2-etilhexil) ftalato y de ion calcio en fermentación sumergida

Phthalates are plastic additives used as plasticizers in the manufacture of flexible plastic materials. These compounds, such as di(2-ethylhexyl) phthalate (DEHP) are toxic substances to the environment and human health, and have been detected as environmental pollutants. In this study, the activity of esterase produced by Fusarium culmorum grown in media supplemented with different concentrations of DEHP (1.5, 3 and 4 g/L) and calcium ion (0.1 and 0.5 g/L) in submerged fermentation was characterized by biochemical tests and polyacrylamide gel electrophoresis. The greatest esterase activity was observed in cultures grown in media added with 3 g of DEHP/L and 0.5 g of calcium ion/L, followed by those cultures grown in media containing 1.5 g of DEHP/L. Whereas cultures grown on 4 g of DEHP/L had the lowest esterase activity. In general, cultures added with 0.5 g of calcium ion/L had the maximum enzymatic activity and the highest enzymatic productivity in the media tested. High concentration of DEHP were used as the sole source of carbon and energy by F. culmorum. It is suggested that the calcium ion favored the esterase production during the DEHP biodegradation. However, further studies are needed to understand the relationship between calcium ion, fungal growth, and enzyme production to provide the appropriate concentration of this ion in the culture medium.

Innovations to overcome the current waste problem caused by single-use plastics in the pursuit of a circular economy

Plastic production continues to increase each year, yet only 9% are successfully recycled. This has impacted natural habitats and ecosystems, due to an uncontrolled amount of waste. The food industry is a major contributor to plastic waste. To counter this problem, the demand for environmentally sustainable alternatives, i.e. bio-based plastics, in the pursuit of a circular economy is increasing. As such, this problem is interconnected and at the resource nexus of particularly, food, material, waste, and ecosystem. This systematic review provides an overview of different innovations regarding materials and additives for bio-based plastics for packaging in the food industry. The paper argues that a majority of materials for bio-based plastics originate from the food industry’s value chain and utilizing these resources is essential to reduce waste and to create more value, essentially addressing the problem with a focus on the resource nexus. Moreover, the importance of developing biodegradable and recyclable plastics to reduce the environmental impact of plastic waste is also highlighted, especially in the context of single-use food packaging. These findings and conclusions cumulated into a framework to differentiate the various materials and classify them regarding their biodegradability properties, origin (plant- or animal-based industry by-products and raw materials) and end-of-life scenarios. This contributes to the academic literature and practice by categorizing different kinds of materials, which might be labelled environmentally sustainable, particularly biodegradable, but which might not always be the case and critically discussing implications of this.

Effect of Glucose Addition on Physical Characteristics of Edible Film from Kappa-Carrageenan Using Sorbitol Plasticizer

The development of increasingly advanced food technology has resulted in various new food product innovations, one example of which is edible film. Edible films can be made from polysaccharides such as kappa carrageenan with the addition of sorbitol and glucose plasticizers to improve their physical characteristics. This research aims to determine the effect of adding glucose on the physical properties of edible film from kappa carrageenan and sorbitol plasticizer. The research carried out was experimental using a Completely Randomized Design (CRD) with four treatments and five replications. Treatment consisted of glucose concentrations of 0%, 0.5%, 1%, and 1.5%. Data analysis used ANOVA, then continued with the DMRT test. The research results prove that glucose has a significant effect (p&lt;0.05) on the physical characteristics of edible film. Optimal results were found at a glucose concentration of 0.5%, with a thickness value of 0.0972 mm, water vapor transmission rate of 3.319 g/m2/day, tensile strength of 3.2648 MPa, and elongation of 22.34%.

Photoaging enhances the leaching of di(2-ethylhexyl) phthalate and transformation products from polyvinyl chloride microplastics into aquatic environments

Increasing chemical pollution is a threat to sustainable water resources worldwide. Plastics and harmful additives released from plastics add to this burden and might pose a risk to aquatic organisms, and human health. Phthalates, which are common plasticizers and endocrine-disrupting chemicals, are released from polyvinyl chloride (PVC) microplastics and are a cause of concern. Therefore, the leaching kinetics of additives, including the influence of environmental weathering, are key to assessing exposure concentrations but remain largely unknown. We show that photoaging strongly enhances the leaching rates of di(2-ethylhexyl) phthalate (DEHP) by a factor of 1.5, and newly-formed harmful transformation products, such as mono(2-ethylhexyl) phthalate (MEHP), phthalic acid, and phthalic anhydride from PVC microplastics into the aquatic environment. Leaching half-lives of DEHP reduced from 449 years for pristine PVC to 121 years for photoaged PVC. Aqueous boundary layer diffusion (ABLD) is the limiting mass transfer process for the release of DEHP from pristine and photoaged PVC microplastics. The leaching of transformation products is limited by intraparticle diffusion (IPD). The calculated mass transfer rates can be used to predict exposure concentrations of harmful additives in the aquatic environment.

Plasticizers levels in four fish species from the Ligurian Sea and Central Adriatic Sea (Mediterranean Sea) and potential risk for human consumption

Plastic materials contain additives such as plasticizers and flame retardants, which are not covalently bound to plastic polymers and can therefore be unintentionally released into the marine environment. This study investigated three families of compounds, phthalates (PAEs), organophosphate esters (OPEs), and non-phthalate plasticizers (NPPs) currently used as plastic additives, in 48 muscle samples of bogue (Boops boops), European hake (Merluccius merluccius), red mullet (Mullus barbatus), and European pilchard (Sardina pilchardus) sampled in the Central Adriatic and the Ligurian Seas. The additional goal of this study is to assess the potential risk to human health from fish consumption with the objective of determining whether the detected levels might potentially pose a concern. PAEs represent the majority of the plastic additives detected in the selected species, with ubiquitous distribution across the study areas, whereas for OPEs and NPPs, there is a more pronounced difference between the two study areas, suggesting that these compounds may represent different exposure levels in the two seas. Among PAEs, bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and diisobutyl phthalate (DIBP) were the most abundant compounds, reaching levels up to 455 ng/g ww. OPEs were detected at higher concentrations in samples from the Ligurian Sea, and triethyl phosphate (TEP) was the most abundant compound. Among the NPPs, acetyl tributyl citrate (ATBC) was most frequently detected. From the results obtained, fish consumption may not pose a risk to human health (Hazard Quotient<1) but needs to be considered in future studies. Given the limited number of studies on PAEs, OPEs and NPPs in the Mediterranean Sea, further research is necessary to understand their potential bioaccumulation in marine organisms.

Effect of plastic additives on anaerobic biological reutilization of biodegradable plastics and sludge: Focusing on the digestion process, microbial composition and metabolic traits

The inevitable release of plastic additives (PAs) from biodegradable plastics (BPs) might affect the ensuing biological reutilization, and the impact of PAs on anaerobic co-digestion performance is still controversial. This study evaluated the impacts of three typical PAs (bis(2-ethylhexyl) phthalate (DEHP), acetyl tributylcitrate (ATBC), tributyl citrate (TBC)) on the BPs anaerobic co-digestion with waste-activated sludge (WAS). Results indicated that the BPs type had a significant effect on methane production, but the effect of PAs was negligible within a concentration range (0.5–50 mg/g TSS). Mechanistic exploration revealed that PAs addition exhibited insignificant effects on solubilization, hydrolysis, and acidification stage. Microbial communities' analyses suggested that PAs did not change the diversity and composition of microbial communities. Furthermore, the addition of PAs has no direct effect on the metabolic function of microorganisms. This study shed light on the effects of PAs on BPs biological reutilization and help to gain a better understanding of the potential environmental hazards of plastics.