Plastic Potential Research Articles

A constitutive model for caprock siltstone subject to three-dimensional compression with emphasis on plastic potential and hardening law

Investigation of the strength, deformation and constitutive model of rocks under true three-dimensional stress is of much significance to scientifically and accurately predict the integrity of underground gas storage caprock. In general the intermediate principal stress and the minimum principal stress to which the caprock is subjected are not equal, and there is uncertainty in the in-situ stress test. In this study, the siltstone is taken from the caprock of the Hutubi gas storage in Xinjiang, with a buried depth of 3600 m. A series of true triaxial compression tests on siltstones were carried out using a true triaxial testing system for rocks, and the strength and deformation characteristics of siltstones under different intermediate principal stress conditions were studied. The elastoplastic constitutive model of rocks has been established: in the plastic part, the Mogi-Coulomb yield criterion has been modified so as to obtain the triple-mean-normal-stress yield function and triple-mean-normal-stress potential function suitable for rocks under the true three-dimensional stress state. The hardening law has been determined by fitting the relationship between the plastic deviatoric shear strain and the deviatoric stress in the true triaxial compression test. The constitutive model and the test data of siltstones have been verified by fitting. Finally, the influence of true triaxial stress on the permeability and breakthrough pressure of caprock siltstones has been discussed. The result indicates that with the increase of intermediate principal stress within a certain range, the peak strength of siltstones will also increase, and deformation in the direction of the intermediate principal stress will be restrained. Compared with the potential functions in other constitutive models, the triple-mean-normal-stress potential function proposed in this paper can accurately describe the plastic flow direction of siltstones under the true three-dimensional stress state. And the hardening law truly reflects the plastic deformation of siltstone specimens under different stress states during the loading process. The calculation result of the constitutive model is in good agreement with the test result. Under the true three-dimensional stress, the permeability of siltstones shows anisotropy. With the increase of the intermediate principal stress, the minimum value of siltstone permeability decreased by 8%, and the maximum value of breakthrough pressure increased by 4.3%.

Yield Surfaces and Plastic Potentials for Metals, with Analysis of Plastic Dilatation and Strength Asymmetry in BCC Crystals

Since the 1980s, constitutive modeling has steadily migrated from phenomenological descriptions toward approaches that are based on micromechanics considerations. Despite significant efforts, crystal plasticity remains an open field of research. Among the unresolved issues are the anomalous behavior of metals at low temperatures and the stress upturn at extreme dynamics. This work is focused on the low-temperature responses of body-centered-cubic (bcc) metals, among them, molybdenum (Mo). At these conditions, the plastic flow strength is governed by the motion of screw dislocations. The resultant non-planarity of core structures and slip causes the following: the shear stress includes non-glide components, the Schmid law is violated, there is a tension-compression asymmetry, and the yield surface and plastic potential are clearly decoupled. We find that the behavioral complexities can be explained by atomistically resolved friction coefficients in macroscopic yield and flow. The plastic flow mechanisms establish the departure point into the follow-up analysis of yield surfaces. For example, we know that while the von Mises stress is explained based on energy considerations, we will also show that the stress has a clear geometric interpretation. Moreover, the von Mises stress is just one case within a much broader class of equivalent stresses. Possible correlations among non-Schmid effects (as represented macroscopically by friction coefficients), volume change (i.e., residual elastic dilatation) from dislocation lines, and elastic anisotropy are investigated. Extensions to the shock regime are also established.

CD8 T-cell diversification: Asymmetric cell division and its functional implications.

Establishment of cellular diversity is a basic requirement for the development of multicellular organisms. Cellular diversification can be induced by asymmetric cell division (ACD), during which the emerging two daughter cells unequally inherit lineage specific cargo (including transcription factors, receptors for specific signaling inputs, metabolic platforms, and possibly different epigenetic landscapes), resulting in two daughter cells endowed with different fates. While ACD is strongly involved in lineage choices in mammalian stem cells, its role in fate diversification in lineage committed cell subsets that still exhibit plastic potential, such as T-cells, is currently investigated. In this review, we focus predominantly on the role of ACD in fate diversification of CD8 T-cells. Further, we discuss the impact of differential T-cell receptor stimulation strengths and differentiation history on ACD-mediated fate diversification and highlight a particular importance of ACD in the development of memory CD8 T-cells upon high-affinity stimulation conditions.

Liquid-Based Memory Devices for Next-Generation Computing

Liquid-based devices have emerged as bioinspired neuromorphic applications owing to their high ion-diffusion coefficients, diverse structures, and controllable ion-exchange reactions. By engineering and modifying liquid materials, multifunctional liquid-based computing devices have been developed for next-generation memory and neuromorphic devices. The unique properties of liquids make them feasible for memory functions and various synaptic applications, such as emulating synaptic plasticity, homeostasis, and action potentials. Utilizing liquids in computing devices provides a promising and versatile platform for high-performance memory devices and enables the emulation of bioinspired computing functions. In this Spotlight, we highlight recent advances in liquid-based memory devices and focus on synaptic applications. We then discuss possible array structures and scaling-down technologies for liquid-based devices. Finally, the challenges and future prospects of liquid-based devices are discussed.

Numerical investigation of plastic strain localization for rock-like materials in the framework of fractional plasticity

The plastic strain localization phenomenon is of critical importance for rock-like materials when undergoing non-homogeneous deformation. This paper presents a fractional plastic framework which is applied to take into account the plastic strain localization of rock-like materials. The novelty of the presented framework lies in the non-coaxial plastic flow that is controlled by the RiemannLiouville (RL) fractional derivative without additional plastic potential. The hardening response is described by a function of the generalized plastic shear strain. The capability of the fractional constitutive model to predict the main mechanical behaviors of rock-like materials is assessed by the conventional triaxial compression test data of Vosges sandstones from the literature. With the help of a MATLAB-based finite element method, several numerical examples (i.e., a plate, a plate with a hole, and a plate with a crack) are implemented, compared and analyzed to demonstrate the effectiveness of the proposed framework and the influence of the fractional order on the strain localization. It can be found from the numerical application of the twin-tunnel that the fractional model is promising to flexibly capture the strain localization zone for rock-like materials.

Ecological plasticity and adaptive potential of annual form of white sweet clover (Melilotus albus Medik)

The ecological plasticity, stability, and adaptive potential of varieties and promising genetic lines of the annual form of white sweet clover were assessed. The studies were carried out in 2016-2020 in Volga Scientific Research Institute of Selection and Seed-Growing named after P.N. Konstantinov, Samara Federal Research Scientific Center, Russian Academy of Sciences. According to the parameters of ecological plasticity and stability, the studied varieties and genetic lines were differentiated into groups according to their response to the changes in environmental conditions. Most of studied varieties belonged to highly plastic genotypes; varieties Srednevolzhsky, Kinelsky, Zavolzhsky, and genetic line Hugh 0 were characterized by the highest stability under the conditions of the Samara Trans-Volga Region. Varieties Srednevolzhsky, Kinelsky, Zavolzhsky, and genetic lines Hugh 0, Hugh 40 and Indus 1 were highly resistant to unfavorable environmental factors. All the studied genetic lines and varieties are characterized by high selective value and thus may be used as a starting material for developing of new varieties of the annual form of white sweet clover.

Assessment of carbon emission potential of polyvinyl chloride plastics

Plastic pollution has become a global concern, and research has shown that carbon emissions during the lifecycle of plastics are rapidly consuming global carbon credits. This study focuses on the effective assessment of carbon emissions from polyvinyl chloride (PVC) plastics using a life cycle assessment (LCA) method during the production and recycling stages. The greenhouse gas emission potential is evaluated using 1kg PVC plastic as a functional unit. Research has shown that the total carbon emissions during the production stage of PVC plastic are 7.83kg CO2-eq. The carbon emissions during the production stage of hydrochloric acid, acetylene, electricity, and water vapor are 2.340 kg CO2-eq, 4.900 kg CO2-eq, 0.117 kg CO2-eq, and 0.468 kg CO2-eq, respectively. During the recycling phase, the carbon emissions from the power consumption zone are 0.184 kg CO2-eq, followed by 0.156 kg CO2-eq from natural gas. Research has shown that fossil materials contribute the largest carbon emissions during the production stage of PVC plastics. Therefore, how to effectively reduce the use of fossil fuels or seek alternative raw materials can effectively reduce carbon emissions.

Projeções futuras e modelagem ecológica para a distribuição de plantas alimentícias não convencionais

ABSTRACT The importance of non-conventional food plants has been evidenced due to their great potential for phenotypic plasticity, resilience and resistance to permanence in inhospitable places. This study aimed to evaluate the natural distribution of two of these species (Eryngium foetidum and Fridericia chica) in the present period (2009-2019) and the projection for two future climate scenarios (RCP 4.5 - “less pessimistic” and RCP 8.5 - “more pessimistic”) in two-time intervals (2020-2050 and 2051-2070), in the six Brazilian phytogeographic domains. Nineteen bioclimatic variables obtained from the WorldClim database and four algorithm models were tested: Climate Space Model, Envelope Score, Niche Mosaic and Environmental Distance. The Environmental Distance algorithm presented the best discrimination of the models adjusted for the two species. From the projections, it is possible to perceive that the species are severely affected in the phytogeographic domains of the Amazon, Pantanal and Pampa, becoming practically extinct in the RCP 8.5 scenario, for the period of 2051-2070.

Degree of plasticity of wheat winter varieties in different ecotypes

Topicality. Over the past decades, a breeding program of leading scientific institutions has been implemented to improve soft winter wheat varieties in terms of yield, grain weight per main spike, degree of plasticity and genotypic effect. These characteristics demonstrate the ability of varieties to adapt to environmental changes. Purpose. To determine the degree of plasticity, level of stability, breeding value of modern varieties of soft winter wheat developed by leading scientific institutions. Materials and Methods. During 2020-2022, 15 varieties of soft winter wheat were examined in the crop rotation of the Breeding and Seed Production Department of the Dnipro State Agrarian and Economic University. Accounting and observations were carried out according to the methodology of state variety testing. Ecological plasticity was determined by the methodology of the Plant Production Institute named after V. Ya. Yuriev of NAAS according to the OSGE application package Tlite Systems gr. Results. The presented varieties have the sum of ranks 2 and 3 for the trait "grain weight per main spike", which indicates their high plasticity due to the stability of genetic potential realisation and their greater adaptation to the growing conditions in the northern subzone of the Steppe of Ukraine. The level of stability, plasticity and breeding value of varieties in different conditions of growing seasons was determined. The varieties Smuhlianka, Podolianka, Peremoha, Ihrysta, Komertsiina, Korusna and Yednist showed the highest level of plasticity. This allows us to predict the realisation of plasticity under sufficient moisture supply during the growing season and a slight decrease it under drought. Conclusions. The stability, plasticity, and breeding value for 15 varieties of soft winter wheat were determined. Under changing environmental conditions, authors revealed the possibility of their genetic potential for yield and ecological plasticity over the years due to the high stability of the genetic effect of the trait. The breeding value of modern varieties in terms of adaptability was confirmed to use them as a source material and introduce into production in conditions of unstable and insufficient moisture supply. Key words: yield, grain weight of the main spike, genotypic effect, stability, breeding value

Mapping of the Human Amniotic Membrane: In Situ Detection of Microvesicles Secreted by Amniotic Epithelial Cells

The potential clinical applications of human amniotic membrane (hAM) and human amniotic epithelial cells (hAECs) in the field of regenerative medicine have been known in literature since long. However, it has yet to be elucidated whether hAM contains different anatomical regions with different plasticity and differentiation potential. Recently, for the first time, we highlighted many differences in terms of morphology, marker expression, and differentiation capabilities among four distinct anatomical regions of hAM, demonstrating peculiar functional features in hAEC populations. The aim of this study was to investigate in situ the ultrastructure of the four different regions of hAM by means of transmission electron microscopy (TEM) to deeply understand their peculiar characteristics and to investigate the presence and localization of secretory products because to our knowledge, there are no similar studies in the literature. The results of this study confirm our previous observations of hAM heterogeneity and highlight for the first time that hAM can produce extracellular vesicles (EVs) in a heterogeneous manner. These findings should be considered to increase efficiency of hAM applications within a therapeutic context.

Prognostic significance of ımmunhistochemical axl expression in pancreas ductal adenocarcinomas.

Pancreas Ductal Adenocarcinomas (PDACs) are among the leading causes of cancer-related death. Tyrosine kinase receptors (TKRs) are responsible for cell plasticity, chemoresistance, immunosuppression and metastasis potential. Axl is a receptor of the TKR family, and it has come to the fore in cancer treatment in the last decade. This study aimed to investigate the relationship of immunohistochemical Axl expression with histological features and its prognostic importance in PDACs. Fifty-three patients who were operated on for PDAC between 2006-2017 were evaluated retrospectively. Features of tumors; size, lymphovascular invasion (LVI), perineural invasion (PNI), resection margin (RM), lymph node metastasis (LNM), differentiation, tumor-infiltrating lymphocyte, stage and overall survival were recorded. Immunohistochemically, membranous and or cytoplasmic staining was considered positive for Axl. Statistically, Pearson Chi-Square, Cox regression and Kaplan Mayer tests were used in the SPSS 21.0 program. Axl was positive in 28 patients (52.8%). Axl positivity was found to be associated with the presence of LVI (P = 0.009) and LNM (P = 0.002) and was an independent prognostic factor in short survival (P = 0.006). It was found that increased expression of Axl, which is known to increase EMT-mediated metastasis in carcinogenesis, may be an indicator of local spread and poor prognosis in PDAC patients. In this respect, it can be promising as a targeted molecule in PDAC patient's individualized treatments.

Leaf acclimation to soil flooding and light availability underlies photosynthetic capacity of Lindera melissifolia, an endangered shrub of bottomland forests in the Mississippi Alluvial Valley, USA.

Lindera melissifolia is an endangered shrub indigenous to the broadleaf forest of the Mississippi Alluvial Valley (MAV). In this region, extant colonies of the species are found in periodically ponded habitats where a diversity of broadleaf trees can form well-developed overstory and sub-canopies-these habitat characteristics suggest that soil flooding and light availability are primary drivers of L. melissifolia ecophysiology. To understand how these two factors affect its photosynthetic capacity, we quantified leaf characteristics and photosynthetic response of plants grown in a large-scaled, field setting of three distinct soil flooding levels (no flood, 0day; short-term flood, 45days; and extended flood, 90days) each containing three distinct light availability levels (high light, 30% shade cloth; intermediate light, 63% shade cloth; and low light, 95% shade cloth). Lindera melissifolia leaves showed marked plasticity to interacting effects of flooding and light with lamina mass per unit area (Lm/a) varying 78% and total nitrogen content per unit area (Na) varying 63% from the maximum. Photosynthetic capacity (A1800-a) ranged 123% increasing linearly with Na from low to high light. Extended flooding decreased the slope of this relationship 99% through a reduction in N availability and metabolic depression of A1800-a relative to Na. However, neither soil flooding nor light imposed an additive limitation on photosynthetic capacity when the other factor was at its most stressful level, and the A1800-a-Na relationship for plants that experienced short-term flooding suggested post-flood acclimation in photosynthetic capacity was approaching the maximal level under respective light environments. Our findings provide evidence for wide plasticity and acclimation potential of L. melissifolia photosynthetic capacity, which supports active habitat management, such as manipulation of stand structure for improved understory light environments, to benefit long-term conservation of the species in the MAV.

Bioprospecting for polyesterase activity relevant for PET degradation in marine Enterobacterales isolates.

Plastics have quickly become an integral part of modern life. Due to excessive production and improper waste disposal, they are recognized as contaminants present in practically all habitat types. Although there are several polymers, polyethylene terephthalate (PET) is of particular concern due to its abundance in the environment. There is a need for a solution that is both cost-effective and ecologically friendly to address this pollutant. The use of microbial depolymerizing enzymes could offer a biological avenue for plastic degradation, though the full potential of these enzymes is yet to be uncovered. The purpose of this study was to use (1) plate-based screening methods to investigate the plastic degradation potential of marine bacteria from the order Enterobacterales collected from various organismal and environmental sources, and (2) perform genome-based analysis to identify polyesterases potentially related to PET degradation. 126 bacterial isolates were obtained from the strain collection of RD3, Research Unit Marine Symbioses-GEOMAR-and sequentially tested for esterase and polyesterase activity, in combination here referred to as PETase-like activity. The results show that members of the microbial families Alteromonadaceae, Shewanellaceae, and Vibrionaceae, derived from marine sponges and bryozoans, are the most promising candidates within the order Enterobacterales. Furthermore, 389 putative hydrolases from the α/β superfamily were identified in 23 analyzed genomes, of which 22 were sequenced for this study. Several candidates showed similarities with known PETases, indicating underlying enzymatic potential within the order Enterobacterales for PET degradation.

Potential of sediment bacterial communities from Manila Bay (Philippines) to degrade low-density polyethylene (LDPE).

The persistence of plastics and its effects in different environments where they accumulate, particularly in coastal areas, is of serious concern. These plastics exhibit signs of degradation, possibly mediated by microorganisms. In this study, we investigated the potential of sediment microbial communities from Manila Bay, Philippines, which has a severe plastics problem, to degrade low-density polyethylene (LDPE). Plastics in selected sites were quantified and sediment samples from sites with the lowest and highest plastic accumulation were collected. These sediments were then introduced and incubated with LDPE in vitro for a period of 91days. Fourier transform infrared spectroscopy detected the appearance of carbonyl and vinyl products on the plastic surface, indicating structural surface modifications attributed to polymer degradation. Communities attached to the plastics were profiled using high-throughput sequencing of the V4-V5 region of the 16S rRNA gene. Members of the phylum Proteobacteria dominated the plastic surface throughout the experiment. Several bacterial taxa associated with hydrocarbon degradation were also enriched, with some taxa positively correlating with the biodegradation indices, suggesting potential active roles in the partial biodegradation of plastics. Other taxa were also present, which might be consuming by-products or providing nourishment for other groups, indicating synergy in utilizing the plastic as the main carbon source and creation of a microenvironment within the plastics biofilm. This study showed that sediment microbes from Manila Bay may have naturally occurring microbial groups potentially capable of partially degrading plastics, supporting previous studies that the biodegradation potential for plastics is ubiquitously present in marine microbial assemblages.

A closest point projection method for stress integration of 3D sand models generalised by transformed stress method

ABSTRACT It is significant to generalise constitutive models from the triaxial space to the multi-axial space for soils. Different from the popular approaches by a g(θ) function to interpolate failure curve, the transformed stress (TS) method can bring the effect of the third stress invariant and stress-induced anisotropy into constitutive models without adding any extra parameters. The closest point projection method (CPPM) is the most popular implicit integration algorithm for the implementation of constitutive model in the finite element analysis. The disadvantage of the CPPM is that it requires the deduction of the quadratic derivative of the plastic potential function, which is extremely difficult for a complex plastic potential. This study employed the TS method with the spatially mobilised plane criterion to generalise a state-dependent sand model, in which the analytical expression of the quadratic derivative of the plastic potential can be obtained. A novel CPPM integration scheme was proposed for the generalised model, which employed an accurate integration of the non-linear elastic constitutive law and a sub-stepping technique to improve the elastic trial state before the Newton’s iteration. The numerical examples demonstrated that the proposed CPPM algorithm has the higher efficiency and better robustness than the conventional cutting plane algorithm.

High Resilience and Fast Acclimation Processes Allow the Antarctic Moss Bryum argenteum to Increase Its Carbon Gain in Warmer Growing Conditions.

Climate warming in Antarctica involves major shifts in plant distribution and productivity. This study aims to unravel the plasticity and acclimation potential of Bryum argenteum var. muticum, a cosmopolitan moss species found in Antarctica. By comparing short-term, closed-top chamber warming experiments which mimic heatwaves, with in situ seasonal physiological rates from Cape Hallett, Northern Victoria Land, we provide insights into the general inherent resilience of this important Antarctic moss and into its adaptability to longer-term threats and stressors associated with climate change. Our findings show that B. argenteum can thermally acclimate to mitigate the effects of increased temperature under both seasonal changes and short-term pulse warming events. Following pulse warming, this species dramatically increased its carbon uptake, measured as net photosynthesis, while reductions in carbon losses, measured as dark respiration, were not observed. Rapid growth of new shoots may have confounded the effects on respiration. These results demonstrate the high physiological plasticity of this species, with acclimation occurring within only 7 days. We show that this Antarctic moss species appears to have a high level of resilience and that fast acclimation processes allow it to potentially benefit from both short-term and long-term climatic changes.

Lymphatic/blood vessel plasticity: motivation for a future research area based on present and past observations.

The lymphatic system plays a significant role in homeostasis and drainage of excess fluid back into venous circulation. Lymphatics are also associated with a number of diseases including lymphedema, tumor metastasis, and various lymphatic malformations. Emerging evidence suggests that lymphatics might have a bigger connection to the blood vascular system than originally presumed. As these two systems are often studied in isolation, several knowledge gaps exist surrounding what constitutes lymphatic vascular plasticity, under what conditions it arises, and where structures characteristic of plasticity can form. The objective of this review is to overview current structural, cell lineage-based, and cell identity-based evidence for lymphatic plasticity. These examples of plasticity will then be considered in the context of potential clinical and surgical implications of this evolving research area. This review details our current understanding of lymphatic plasticity, highlights key unanswered questions in the field, and motivates future research aimed at clarifying the role and therapeutic potential of lymphatic plasticity in disease.

Determining the Feasibility of a Circular Economy for Plastic Waste from the Construction Sector in New Zealand

By 2012, the annual quantity of C&D waste produced by 40 countries had reached three billion tonnes, contributing 10-50% to total municipal solid waste around the world. Recent data from Australia and New Zealand estimated a combined contribution of approximately 28 million tonnes C&D waste to landfill for just 0.4% of the world’s population. If C&D waste was produced at an equal rate around the world, global production could be close to seven billion tonnes. In 2015, the global production of plastic waste from building and construction was 13 million tonnes. It is estimated that annually, C&D waste contributes ≥25,000 tonnes to the total amount of plastic landfilled in one major city (Auckland) alone. Waste audits from four sites demonstrated that this was predominantly polyethylene (PE) or polyvinyl chloride (PVC), and was derived from packaging, building componentry and building protection equally. The aims of this study were to implement a ‘foundations to completion’ plastic waste audit on a new secondary school in Auckland, New Zealand. This sheds light on the nature of the plastic waste, e.g., type, use and potential for recyclability or reuse. The aim was to also identify the challenges in the construction industry that hinder effective waste diversion from landfill, and to trial practical on-site solutions.

Marine-Derived Actinomycetes: Biodegradation of Plastics and Formation of PHA Bioplastics-A Circular Bioeconomy Approach.

Plastics are present in the majority of daily-use products worldwide. Due to society's production and consumption patterns, plastics are accumulating in the environment, causing global pollution issues and intergenerational impacts. Our work aims to contribute to the development of solutions and sustainable methods to mitigate this pressing problem, focusing on the ability of marine-derived actinomycetes to accelerate plastics biodegradation and produce polyhydroxyalkanoates (PHAs), which are biodegradable bioplastics. The thin plastic films' biodegradation was monitored by weight loss, changes in the surface chemical structure (Infra-Red spectroscopy FTIR-ATR), and by mechanical properties (tensile strength tests). Thirty-six marine-derived actinomycete strains were screened for their plastic biodegradability potential. Among these, Streptomyces gougerotti, Micromonospora matsumotoense, and Nocardiopsis prasina revealed ability to degrade plastic films-low-density polyethylene (LDPE), polystyrene (PS) and polylactic acid (PLA) in varying conditions, namely upon the addition of yeast extract to the culture media and the use of UV pre-treated thin plastic films. Enhanced biodegradation by these bacteria was observed in both cases. S. gougerotti degraded 0.56% of LDPE films treated with UV radiation and 0.67% of PS films when inoculated with yeast extract. Additionally, N. prasina degraded 1.27% of PLA films when these were treated with UV radiation, and yeast extract was added to the culture medium. The main and most frequent differences observed in FTIR-ATR spectra during biodegradation occurred at 1740 cm-1, indicating the formation of carbonyl groups and an increase in the intensity of the bands, which indicates oxidation. Young Modulus decreased by 30% on average. In addition, S. gougerotti and M. matsumotoense, besides biodegrading conventional plastics (LDPE and PS), were also able to use these as a carbon source to produce degradable PHA bioplastics in a circular economy concept.

Abstract A013: Induced pluripotent stem cells derived from normal human esophageal epithelial cells identify transcription factor networks contributing to esophageal carcinogenesis

Abstract Esophageal cancer (EsC) is the sixth leading cause of cancer-related mortality worldwide. Esophageal squamous cell cancers (ESCC) typically arise in the upper and mid-esophagus, whereas esophageal adenocarcinomas (EAC) arise in the distal esophagus and gastroesophageal junction. Presently, epigenetic mechanisms contributing to initiation, progression, and dissemination of EsC have not been fully elucidated. The present study was undertaken to determine if induced pluripotent stem cells (iPSCs) derived from normal esophageal epithelial cells (Eso-iPSCs), EsC cell lines cultured in stemness enriched conditions, and cancer stem-like cells isolated from primary EsC specimens could be used to identify unique primitive stem-like transcription factor networks associated with epigenetic plasticity, chemoresistance, and metastatic potential of EsC cells. RNA-seq analysis demonstrated that genes differentially expressed in Eso-iPSC overlapped with transcriptome signatures in lung-iPSC (Lu-iPSC) which we previously generated to identify novel therapeutic targets in lung cancers; similar to Lu-iPSC, Eso-iPSC transcriptome signatures overlapped considerably more with small cell lung cancers (SCLC) compared to non-small cell lung cancers (NSCLC), possibly reflecting greater stemness in SCLC. We next compared transcriptome signatures of Eso-iPSC with stem-like-EAC enrichment models (cell lines and PDX). 6% and 8% of differentially expressed genes in Eso-iPSC uniquely overlapped with EAC or ESCC lines, respectively, whereas 24% were common to Eso-iPSC, EAC cells, and ESCC cells. Culturing of EAC cells on matrigel or propagation of EAC as primary patient derived xenografts increased commonalities of Eso-iPSC and EAC transcriptome signatures. Lastly, we compared transcriptomes of Eso-iPSC with Barrett’s esophagus (BE; a premalignant condition in which the squamous epithelium of the distal esophagus is replaced by metaplastic columnar epithelial cells) and EAC samples. By combining and stringently filtering transcriptome, ATAC-seq, DNA methylome, and ChIP-seq datasets for tissue- and disease-specific signatures, we identified several potentially druggable, master TFs mediating stemness/plasticity, chemoresistance, and metastatic potential which are shared between EsC lines and primary tumor specimens. Enrichment techniques promoted acquisition of more stem-like states in cultured EAC cells as reflected in multi-omic signatures of these cells relative to Eso-iPSC and primary EAC specimens. Collectively, these findings establish proof of principal for our experimental approach which is now being used to further characterize epigenetic mechanisms contributing to esophageal carcinogenesis and to identify novel targets for EsC therapy. Citation Format: Haitao Wang, Ruihong Wang, Katherine P. Prothro, Sichuan Xi, Weilong Hou, Xinwei Wu, Tuana Tolunay, Vivek Shukla, Mary R. Zhang, Stephanie J. Shiffka, Sudheer Gara, David S. Schrump. Induced pluripotent stem cells derived from normal human esophageal epithelial cells identify transcription factor networks contributing to esophageal carcinogenesis. [abstract]. In: Proceedings of the AACR Special Conference: Cancer Epigenomics; 2022 Oct 6-8; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2022;82(23 Suppl_2):Abstract nr A013.