Plasma Rich Fibrin Research Articles

Procedures for production, storage and utilization of platelet-rich plasma and correlated products in Brazil: a cross-sectional study

Introdução: O plasma rico em plaquetas (PRP) é uma tecnologia com potencial aplicação em diversas áreas da medicina e odontologia. A alta variabilidade no processo de produção desta tecnologia e de tecnologias relacionadas desafiam a regulamentação de sua produção, do controle de qualidade e do uso. Objetivo: Avaliar práticas de produção, armazenamento e utilização do PRP e produtos relacionados no Brasil, permitindo a identificação de variabilidade operacional ao longo destes processos. Método: Estudo transversal com coleta de dados realizadas por meio de um questionário eletrônico, autoaplicado e com preservação de anonimato. As perguntas foram definidas a partir de revisão de literatura e por consulta a especialista da área. O questionário foi encaminhado a pesquisadores e a profissionais de várias áreas atualmente envolvidas na produção do PRP. Esses profissionais foram identificados por diferentes estratégias, tais como participação prévia em eventos relacionados e publicações na área. Resultados: Foram obtidas 64 respostas, sendo 36 respostas completas (56,3%) e 28 respostas parciais (43,7%). A taxa de respondentes não pôde ser calculada, considerando-se as formas de divulgação do questionário. Para a produção de PRP, as respostas indicaram predomínio de protocolos com centrifugação dupla e utilização de métodos variados para ativação plaquetária. A maioria dos respondentes referiu utilizar o PRP nas áreas de ortopedia, fisiatria e medicina esportiva. Para a produção do plasma rico em fibrina (PRF), houve predomínio de respostas indicando utilização de protocolo de centrifugação única, sem utilização de métodos de ativação plaquetária. A maioria dos respondentes indicou utilizar o PRF em áreas da odontologia. No geral, observou-se grande variabilidade nos procedimentos de obtenção e do perfil de utilização entre os principais tipos de concentrados de plaquetas. Conclusões: Os achados corroboram a necessidade do desenvolvimento de medidas regulatórias com foco nos pontos críticos identificados, visando promover a segurança durante a produção e utilização dos concentrados de plaqueta.

Assessment of Bone Regeneration Using Tooth Ash and Injectable Platelet Rich Fibrin: A Microcomputed Tomographic (CT) Analysis

Introduction: One of the main challenge of bone graft or socket preservation in particular is to get good quality and quantity of bone in short time prior to implant bed preparation. The buccal bone at the crest of the ridge is a very thin bone and usually resorb faster than the rest of alveolar bone which may hinder an optimum dento-osseous implant insertion. The purpose of this study will be to assess the bone regeneration capabilities of Tooth Ash Particles (TAP) with injectable Plasma Rich Fibrin (i-PRF) and Tooth Ash Particles (TAP) alone at defects created in the goat mandible bone using micro-computed tomographic (micro-CT). Materials and Methods: A total, 54 bone defect (5 mm × 8 mm) were performed in the 18 goats. The created defect received different treatment (Tx): Tx.A: Unfilled defect (allow natural bone regeneration; Tx.B: Tooth Ash particle (TAP) alone; Tx.C: Tooth Ash + injectable PRF (TAP/i-PRF). Six goats, were sacrificed at different time points:Group 1: at 2nd week, Group2: at 5th week and Group3 at 8th week. The newly formed bone (NFB) was analyzed using micro-CT at different time points. Quantitative and qualitative assessment were carried out namely; the volume of new bone formation (NF-BV) within the defect and its mineral density (NF-BMD), Trabecular Thickness (Tb Th), Trabecular Number (Tb N) and Trabecular Separation (Tb Sp). Result: By 8th week, the mean NF-BV was 69.482 ± 6.554 mm3 (cubic millimeter), 65.872±6.804 mm3, 26.820±14.643 mm3, while the mean NF-BMD was 0.417±0.119g/mm3, 0.786±0.036 g/mm3, 0.805±0.033 g/mm3 for the defects which received Tx.C, Tx.B and Tx.A respectively. At 8th weekTb Th of NFB was 0.612±0.168, 0.913±0.112, and 0.701 ±0.126, Trabecular Number of NFB was 2.062±0.946, 1.002±0.155, and 1.816±2.042 and, Trabecular Separation of NFB was 0.330 ±0.131, 0.559 ±0.110, and 0.495 ±0.258 for the defects which received Tx.A, Tx.B and Tx.C respectively. Conclusion: Micro-CT study demonstrated that tooth ash particles mixed with injectable Platelet Rich Fibrin (i-PRF) on mandibular bone defect in goat’s model, resulting in new bone with significantly higher volume, mineral density and less remodeling rate when compared with normal bone regeneration of unfilled defects.

Treatment of Miller I Mandibular Gingival Recessions Using PRF vs. Connective Graft.

Gingival recession (GR) can cause aesthetic and functional problems. Using connective tissue graft (CTG) and coronally advanced flap (CAF) is considered the technique of choice for treating GR. Considering the morbidity resulting from taking CTG, different alternative biomaterials have been described, including plasma-rich fibrin (PRF) membrane. Studies in lower teeth are few because of the complexity of the factors that can influence obtaining less predictable outcomes. Objective. To compare between CAF + PRF and CAF + CTG in the treatment of lower teeth Miller I gingival recession. Methodology. Split-mouth included 26 isolated GR (13 in each side of the mouth). The left side was treated with CAF + PRF and the right side with CAF + CTG. Clinical variables, probing depth (PD), GR, keratinized tissue (KT), vestibular soft tissue thickness (VSTT), and teeth sensitivity (TS), were assessed at the baseline. GR, KT, VSTT, extraoral inflammation (EI), and patient discomfort (PaD) were assessed at 45 days. Results. Statistically greater VSTT at 45 days was obtained using CAF + CTG (p < 0.05). Less EI and PaD were obtained using CAF + PRF (p < 0.05). No change was observed in GR, KT, and TS values in the intergroup or intragroup comparisons. Conclusion. Even with the limitations of this study, using PRF and CTG in lower teeth demonstrated an improvement in terms of root coverage, although it was without a total percentage of coverage. Regarding the VSTT, better results were obtained using the CTG + CAF, suggesting eventually long-term stable clinical results. We suggest a combined technique for future investigations.

INFLUENCE OF MESENCHYMAL STEM CELLS AND FIBRIN RICH PLASMA MEMBRANE TO REPAIR OSTEOCHONDRAL DEFECTS IN THE RABBIT KNEE

<h3>Background</h3> Bone marrow-derived mesenchymal stromal cells (BMMSC) can be expanded ex vivo, they have the ability to regenerate cartilage for accelerated knee recovery as already demonstrated in several studies. This study evaluated the influence BMMSC and fibrin-rich plasma membrane (FRPM) to repair osteochondral defects in the rabbit knee. <h3>Methods</h3> We evaluated 51 New Zealand male and female rabbits of average weight 4 kg and seven months. The animals were randomly separated into five groups: Group 0 (immediate control): 7 animals submitted to removal of cartilaginous flap until exposure of the subchondral bone of femoral trochlea, by abrasion sulcoplasty of 0.3 (width) x 0,9 (length) cm; Group A (control 60 days): 11 animals submitted to the same procedure as Group 0; Group B (stem cells): 11 animals submitted to the same Group 0 procedure and 5 × 106 BMMSC transplantation at the lesion site; Group C (fibrin-rich plasma membrane): 11 animals submitted to the same procedure of Group 0 and application of MPRF on the lesion and Group D (BMMSC + FRPM): 11 animals submitted to the same procedure of Group 0 and transplantation of 5 × 106 of BMMSC at the site of injury, covered with FRPM. Group 0 animals were submitted to euthanasia shortly after removal of the cartilaginous flap, while the animals of Groups A, B, C and D were submitted to euthanasia after 60 days of cartilage flap removal and established therapy in Groups B, C and D. <h3>Results</h3> It evaluated by morphometry and histological analysis, if there was a decrease in lesion length and an increase in the density of chondrocytes and chondroblasts in the regenerated tissue and adjacent tissue. In the present study, it was observed that the association of the treatments was superior to the isolated treatments regarding the reduction of the iatrogenic lesion. In all groups the chondrocyte density of both regenerated tissue and adjacent tissue was greater than the density observed in Group 0, whereas the chondroblasts density in all groups was lower than the density observed in Group 0. Even without statistical difference, the association of BMMSC and FRPM presented a more uniform histological evaluation. <h3>Conclusion</h3> The association of BMMSC and FRPM was more efficient in decreasing the iatrogenic injury than the natural repair and their isolated administration.

Main Approaches to the Use of Biomaterials in Oral and Maxillofacial Surgery: A Brief Systematic Review

&#x0D; &#x0D; &#x0D; &#x0D; Introduction: In recent years, procedures with the use of dental implants have increased worldwide, reaching approximately one million dental implants per year. In recent years, a platelet concentrate called FRP (fibrin-rich plasma) has been the subject of clinical studies. Associated with this, the biomaterial Bio-Oss® (Geistlich), as it is biodegradable, biocompatible, non-toxic, and has low immunogenicity, and bio stimulators can act in the regeneration of bone tissue, as it establishes with the cells the appropriate biological niche (favorable microenvironment) for bone growth. Objective: Therefore, the present study aimed to evaluate, through a brief systematic review, the results that involve bone formation for dental implantation, with the use of biomaterials such as fibrin-rich plasma and Bio-Oss®. Methods: The model used for the review was PRISMA. Was used databases such as Scopus, Scielo, Lilacs, Google Scholar, PubMed. Results: Fibrin-rich plasma (FRP) as an autologous biomaterial for use in oral and maxillofacial surgery presents most leukocytes, platelets, and growth factors, forming a fibrin matrix, with three-dimensional architecture. The Bio-Oss® biomaterial (Geistlich), as it is biodegradable, biocompatible, non-toxic, and has low immunogenicity and bio stimulators can act in the regeneration of bone tissue, since it establishes with the adenomatous mesenchymal stem cells the appropriate biological niche for bone growth and, thus, allowing the dental implant to be as effective as possible. Conclusion: The use of FRP associated with Bio-Oss® seems to illustrate high success rates with minimal costs, which may reduce the amount of bone graft needed to fill the sinus cavity, reducing the costs of the procedure.&#x0D; &#x0D; &#x0D; &#x0D;

Dental Implants and Plasma Rich in Fibrine: A Wide Literary Review

&#x0D; &#x0D; &#x0D; &#x0D; Introduction: When a dental element is lost in the posterior region of the maxilla, there is natural reabsorption of the alveolar process and at the same time there will be pneumatization of the maxillary sinus. It will increase its volume towards the place where the roots existed and this will often make it difficult or impossible to restore implants in place. For this reason, the procedure for elevating the floor of the maxillary sinus or short implants should be performed when possible. In this context, allogeneic, xenogenous, and alloplastic bone grafts are an alternative for the treatment of bone defects in the jaws, since they avoid the need for a second surgical access. However, due to the need for processing to eliminate antigenic components, these grafts are only osteoconductive with a lower bone formation potential compared to autogenous bone grafts. Also, in this context, in the last 20 years, platelet concentrates have been proposed as regenerative materials in tissue regeneration procedures. Among the platelet concentrates proposed in the literature, PRP and FRP are found to act as autogenous platelet aggregates with osteoinductive properties. Objective: The present study aimed to conduct a wide literature review on maxillary sinus surgery using fibrin-rich plasma. Methods: Experimental and clinical studies (case reports, retrospective, prospective and randomized) with qualitative and/or quantitative analysis were included. Results: The total of 48 articles were found involving Maxillary sinus surgery, Fibrin-rich plasma, and Biomaterials, of which 22 were selected to compose the present study. Conclusion: Based on the literary findings, it was shown that the FRP is favorable for bone formation processes for dental implants, especially when combined with xenografts.&#x0D; &#x0D; &#x0D; &#x0D;

Bone formation with functionalized 3D printed poly-ε-caprolactone scaffold with plasma-rich-fibrin implanted in critical-sized calvaria defect of rat

Background/purposeSpace-making is one of the essential factors for bone regeneration in severe bony defect. To test the hypothesis that an appropriately designed scaffold may be beneficial for the bone formation in defect, the new bone formed in the critical-size calvarial defect of rat was examined after implanted with a 3D-printed poly-ɛ-caprolactone (PCL) scaffold, retaining with and without plasma rich fibrin (PRF). Materials and methodsThirty-two rats were divided into four groups (control, PCL, PRF, and PCL-plus-PRF). A custom-made 3D-printed PCL scaffold, 900 μm in pore size, retaining with and without PRF, was implanted into a critical-sized calvarial defect, 6 mm in diameter. Animals were sacrificed at week-4 or 8 after implantation for assessing the new bone formation by dental radiography, micro-computed tomography (μ-CT), and histology. ResultsBy radiography and μ-CT, significantly greater mineralization areas/volumes were observed in defects with 3D-printed scaffold groups compared to that without the scaffold in both two-time points. However, no advantage was found by adding PRF. Histology showed that bone tissues grew into the central zone of the critical defect when 3D-printed PCL scaffold was present. In contrast, for the groups without the scaffolds, new bones were formed mostly along defect borders, and the central zones of the defects were collapsed and healed with thin connective tissue. ConclusionOur results suggest that the use of a 900 μm pore size 3D-printed PCL scaffold may have the potential in facilitating the new bone formation.

Evaluation and Comparison of Human Chorionic Amniotic Membrane and Platelet-Rich Fibrin in Achieving Bone Formation and Soft Tissue Healing in Extraction Sockets Indicated for Rehabilitation with Implants: A Preliminary Study.

To evaluate and compare human chorionic amniotic membrane and platelet-rich fibrin on new bone formation and soft tissue healing in extraction sockets indicated for rehabilitation with dental implants. A prospective, triple blind clinical study was conducted. The inclusion criteria were as follows: patient with two extraction sites each in the same arch, intact buccal bone and soft tissue around the socket, and recommended rehabilitation with dental implants. Postextraction, the sockets were randomly placed with human chorionic amniotic membrane in one site and platelet-rich fibrin in the other site. After 3 months, a trephine drill was used to take a biopsy of the respective sites for soft and hard tissue samples. The outcome parameters that were assessed histologically were percentage of new bone formation and lymphocyte density. After screening 80 patients, eight participants were recruited for the study. The mean percentage of new bone formation in the human chorionic amniotic membrane group was 45.71% ± 4.82%, and for the plasma-rich fibrin group, it was 41.39% ± 6.29%, showing no statistically significant difference (z = 0.99, P = .31). In the human chorionic amniotic membrane group, six out of eight sites had mild lymphocyte density, while the plasmarich fibrin group had equal numbers of mild and moderate lymphocyte density. No statistically significant difference between the groups (Fischer test value = 0.60, P = .25) was noted. Within the limitations of the study, the results showed that there is no difference in the efficiency of human chorionic amniotic membrane compared with platelet-rich fibrin in achieving new bone formation and soft tissue healing in the extraction socket.

Clinical and Radiographic Evaluation of the Labial Bone Plate after Using Plasma Rich Fibrin in Immediate Implant in Esthetic zone

Clinical and Radiographic Evaluation of the Labial Bone Plate after Using Plasma Rich Fibrin in Immediate Implant in Esthetic zone

A Histomorphometric meta-analysis of sinus elevation with various grafting materials

Several grafting materials have been used in the sinus floor augmentation procedures including autogenous bone(AB), Xenograft (Bio-Oss), inorganic bovine bone (ABB), platelet rich fibrin (PRF) , plasma rich fibrin (PRF), hydroxy appatite (HA), calcium sulfate and pegen P15 used AB as a comparator and the other six materials as interventions. Up to now a subject of controversy in maxillofacial surgery and dentistry is what is the most appropriate graft material for sinus floor augmentation. Materials and Methods: The literature searches were performed using PubMed search. The search covers only English, human and RCT literatures. For analyzing the quality and quantity of bone. After search strategy on PubMed we found 336 articles then after applying the inclusion and exclusion criteria the remaining RCT articles which met the criteria are 2 studies, which measures the bone quality only by Histomorphometric and no included RCT paper using CBCT. Results: In the remaining 2 articles the comparison occur between 2 materials which are autogenous bone and Bio-Oss using Histomorphometric analysis on 48 patients, which gives result in the first study AB=37.7 ± 31.3%, Bio-Oss=41.7 ± 26.6% and in the second study AB=42.74 ± 2.10%, Bio-Oss=24.90 ± 5.76%. Conclusion: The bone quality formed by Bio-Oss is less than autogenous bone by 17.1% so that the autogenous bone remains the gold standard grafting material.

Association of subepithelial connective tissue graft and plasma rich-fibrin for treatment of gingival recession – clinical case report with 12 months post-surgical follow-up

Gum is a portion of periodontal tissue that plays an important role in protecting the surface of the teeth. Its migration beyond the boundaries of the cementum enamel junction, exposing the root of the tooth, is called Gingival Recession (GR) which increases susceptibility to root caries, dentin sensitivity, and aesthetic discomfort. Objective: The main goal of this case report is to describe a surgical procedure that combined the technique of subepithelial connective tissue graft and Plasma-rich fibrin for treatment of gingival recession. Case report: A man, 45 years old, leucoderma, attended the Positive University dental clinic complaining that ‘his gum was rising, and his teeth were sensitive’. Clinical buccal examination was performed according to international guidelines for periodontal data collection and was the man was diagnosed with GR in the region comprising the buccal faces of the first right upper molar to the first right premolar. Before surgical procedure, 60 ml of blood Gum is a portion of periodontal tissue that plays an important role in protecting the surface of the teeth. Its migration beyond the boundaries of the cementum enamel junction, exposing the root of the tooth, is called Gingival Recession (GR) which increases susceptibility to root caries, dentin sensitivity, and aesthetic discomfort. Objective: The main goal of this case report is to describe a surgical procedure that combined the technique of subepithelial connective tissue graft and Plasma-rich fibrin for treatment of gingival recession. Case report: A man, 45 years old, leucoderma, attended the Positive University dental clinic complaining that ‘his gum was rising, and his teeth were sensitive’. Clinical buccal examination was performed according to international guidelines for periodontal data collection and was the man was diagnosed with GR in the region comprising the buccal faces of the first right upper molar to the first right premolar. Before surgical procedure, 60 ml of blood

Functional Medicine-Based Dentistry: A Clinical Conversation with Mary Ellen S. Chalmers, DMD, and Robert Rountree, MD

Functional Medicine-Based Dentistry: A Clinical Conversation with Mary Ellen S. Chalmers, DMD, and Robert Rountree, MD

Regenerative Endodontics: A Potential Solution for External Root Resorption (Case Series)

IntroductionRegenerative endodontic procedures (REPs) are aimed to treat apical periodontitis and promote root maturation of immature necrotic teeth. However, REPs are not intended to be a primary indication for treating or arresting external root resorption (ERR). The purpose of this study was to describe REP treatment in the cessation of ERR. MethodsFour cases (5 teeth) of posttraumatic immature teeth diagnosed with necrotic pulp and apical periodontitis or chronic apical abscess were treated with REPs using plasma-rich fibrin as a scaffold. All the teeth showed ERR and have been followed up to 3 years. ResultsThis case series shows how REPs arrested ERR. In 3 of the cases, replacement resorption was arrested, thus avoiding complications of ankylosis and the need for decoronation. ConclusionsIn addition to the known advantages of REPs, we show that REPs are a promising treatment modality for arresting ERR, warranting further clinical trials.

Targeted Endodontic Microsurgery: Computed Tomography–based Guided Stent Approach with Platelet-rich Fibrin Graft: A Report of 2 Cases

Endodontic microsurgery is a predictable alternative to nonsurgical treatment of persistent and recurrent apical periodontitis. The modern endodontic microsurgical procedure has been evolving in pace with technological advances. The introduction of modern innovations such as cone-beam computed tomographic (CBCT) imaging, 3-dimensional (3D) printing technology, and a 3D surgical guide designed with computer-aided software has allowed for a novel approach to the surgical site. The aim of these case reports was to describe root-end surgery with the use of the implant DDS-Pro planning software (Natrodent Polska, Poland), a 3D-printed surgical guide precisely positioned according to the preoperative CBCT scan measurements (CS 8100; Carestream Dental, Atlanta, GA), and a modified soft tissue access. A hollow trephine bur was used to perform the osteotomy, resection of the root, and enucleation of the lesion. The intact cortical plate was salvaged and used as a graft along with plasma-rich fibrin acquired preoperatively from the patient’s blood. The positioning guide allowed the clinicians to precisely achieve targeted tissues and shorten the procedure time. Modified soft tissue management helped achieve a small surgical wound for uneventful healing. A less than 12-month CBCT follow-up of both cases showed complete 3D healing of the surgical site.

Periodontal Therapy Associating Plasma Rich in Fibrine (PRF) in treating teeth with furca injury grade II: literature review

Periodontal treatment for teeth with a furcation lesion is a challenge for the dental surgeon due to the remaining tissue complexity, limiting the prognosis of the therapy. The assumption of this study is that auxiliary therapy with regenerative approaches could increase the chances of clinical success. Thus, the objective was to evaluate the effect of the combination of Scraping and Root Straightening (RAR) and Fibrin Rich Plasma (PRF) in the treatment of grade II furcation injury. The systematic review was carried out following the PRISMA criteria, including the PICO strategy for the study design. The PubMed, Scopus, Cochrane DataBase, SciELO, LILACS databases were used. The search strategy included the terms MeSH and DeCS and was carried out in September 2019. With this research, it was observed that the studies available in the literature do not present a standardization in the study of PRF for treatments of grade II furcation injuries. Therefore, in this review, we cannot state that PRF is the gold standard for treating furcation injuries, although statistically it presents more satisfactory results. We emphasize the importance of further studies associating RAR and PRF in a more judicious manner and with more methodologies of investigation such as histological, morphological and image analyzes in studies to assess the validation of tissue neoformation in the treatment of grade II furcation injury.

Plasma-rich fibrin in neurosurgery: a feasibility study.

Cerebrospinal fluid (CSF) leakage represents an important and sometimes challenging complication in both cranial and spinal surgery. Current available options for dural closure pose inherent problems regarding safety, efficacy, immunogenicity, cost, and invasiveness. In this article, the use of leukocyte- and platelet-rich fibrin (L-PRF) derived from the patient's own blood is proposed to facilitate dural closure. We aim to describe the safety, feasibility, and applicability of L-PRF membranes and plugs in cranial and spinal neurosurgery. A retrospective study reviewing clinical and surgical characteristics was conducted in 47 patients in whom the use of L-PRF was attempted to reinforce dural closure at a single institution during 1year. Procedures included skull base, posterior fossa, and spinal revision surgeries. L-PRF membranes and/or plugs were used in 44 surgeries. The preparation of L-PRF failed in three cases. L-PRF membranes were used as onlay grafts to augment sealing or sutured into a defect. No short-term complications related to the use of L-PRF were recorded. Postoperative CSF leakage was present in two endoscopic transsphenoidal pituitary surgeries and in one spinal CSF leak repair. L-PRF is safe, inexpensive, and completely autologous and can be rapidly and non-invasively harvested to aid in dural closure. Theoretical advantages include a regenerative bioactive potential, which could lead to improved wound healing and reduced infection rates. These findings warrant larger prospective studies to determine the potential role of L-PRF in neurosurgery.

Closure of Oroantral Communication With Plasma-Rich Fibrin Membrane.

Oroantral communication (OAC) is the opening between the maxillary sinus and oral cavity. It may cause oroantral fistula or maxillary sinusitis if left untreated. The surgical closure of the OAC within 48 hours was recommended to avoid the complications like sinus infections. The aim of this study is to evaluate the treatment of OACs with plasma-rich fibrin (PRF) which is safe and easy to implement in the OACs.This study was conducted with the patients, who required the treatment of the OAC, which was developed after the posterior maxillary tooth extraction in the Dental and Maxillofacial Department of the Faculty of Dentistry in Adnan Menderes University.Plasma-rich fibrin membranes were inserted in layers into the tooth socket so that they covered the OAC. Then these membranes were fixated with the sutures to the surrounding gingiva. Antibiotic (amoxicillin/clavulanic acid 1000 mg), analgesic (dexketoprofen trometamol and/or paracetamol), and oral rinse (0.2% chlorhexidine digluconate) agents were prescribed to all patients. The patients were examined in the 3rd and 7th days and 2 months after the operation.All patients tolerated PRF perfectly, and the soft tissue recovery was completed without any problem. Full epithelization was observed in the defect area in all patients. The OAC did not relapse in any patient.Plasma-rich fibrin technique is a simple and effective method, which can be used in the treatment of OACs with a diameter of 5 mm or less with a low risk of complications.

Treating Odontomes : Surgical Management with Plasma Rich Fibrin as New Front Foot

Treating Odontomes : Surgical Management with Plasma Rich Fibrin as New Front Foot

Platelet-Rich fibrin as palatal bandage: A case report

Introduction PRF [Plasma-rich fibrin] consists of numerous growth factors and platelet cytokines that promote faster wound healing. The use of PRF bandage on donor site in FGGs [free gingival graft procedures] can significantly reduce morbidity and accelerate healing. The aim of the presented case report is to document the contribution of PRF in the healing of FGG donor site.Method and MaterialPRF was prepared and compacted as a dressing on the palatal wound. Post-operative follow-up was done every week for 1 month.Results and DiscussionIt was observed that wound healing was superior and accelerated compared to conventional FGG. Complete wound healing was observed in 2 weeks. The patient reported minimal pain during first 2 days post-operatively.Owing to the promising results delivered, a PRF palatal bandage is an efficacious method to safeguard the raw wound engendered as a result of FGG remarkably alleviating discomfort afflicted patients.

Proliferation and differentiation of stem cells in contact with eluate from fibrin-rich plasma membrane

ObjectiveTo evaluate the ability of the eluate from fibrin-rich plasma (FRP) membrane to induce proliferation and differentiation of isolated human adipose-derived stem cells (ASCs) into chondrocytes. MethodFRP membranes were obtained by centrifugation of peripheral blood from two healthy donors, cut, and maintained in culture plate wells for 48h to prepare the fibrin eluate. The SCATh were isolated from adipose tissue by collagenase digestion solution, and expanded in vitro. Cells were expanded and treated with DMEM-F12 culture, a commercial media for chondrogenic differentiation, and eluate from FRP membrane for three days, and labeled with BrdU for quantitative assessment of cell proliferation using the High-Content Operetta® imaging system. For the chondrogenic differentiation assay, the SCATh were grown in micromass for 21 days and stained with toluidine blue and aggrecan for qualitative evaluation by light microscopy. The statistical analysis was performed using ANOVA and Tukey's test. ResultsThere was a greater proliferation of cells treated with the eluate from FRP membrane compared to the other two treatments, where the ANOVA test showed significance (p<0.001). The differentiation into chondrocytes was visualized by the presence of mucopolysaccharide in the matrix of the cells marked in blue toluidine and aggrecan. ConclusionsTreatment with eluate from FRP membrane stimulated cell proliferation and induced differentiation of the stem cells into chondrocytes, suggesting a potential application of FRP membranes in hyaline cartilage regeneration therapies.