Plasma Malondialdehyde Levels Research Articles

In ovo injection of cinnamon or clove alters the physiology and growth of broilers in a hot tropical environment

A study was conducted to evaluate the influence of in ovo feeding of clove and cinnamon on broilers. The experiment used 700 broiler (Ross 308) hatching eggs that were incubated at the recommended temperature of 37.8 °C. On day 17.5 of incubation, 100 eggs were randomly assigned to each of the following seven treatments: uninjected eggs (OE), eggs injected 0.5 mL distilled water (DH), 2 mg of clove, 4 mg of clove, 2 mg of cinnamon, 4 mg of cinnamon, and 3 mg of ascorbic acid (AC). During the posthatch period, the chicks were raised for 56 days. Data on physiological parameters, growth performance, and intestinal histomorphology were collected. Results revealed that the plasma triiodothyronine (T3) of AC and CV2 chicken was higher than the others. Additionally, the plasma malondialdehyde levels of the chickens of AC, CV2, and CM2 were improved significantly (P < 0.05). The initial weights of CV2 birds were comparable with AC CV4, CM4, and CM2 birds but heavier than those of OE and DW. The bodyweight gain in the CV2 group was similar to AC, CV4, and CM2 groups but heavier than OE, DW, and CM4 birds. Feed intake of OE and DW groups was similar to AC, CV2, CV4, and CM2 but higher than CM4. The feed conversion ratio of OE and DW chickens was comparable but higher than the value obtained in chickens of other treatments. The intestinal morphology of the birds did not follow a particular trend. The study concluded that the in ovo injection of 2 mg of clove improved broiler birds' metabolic and antioxidant status at hatch. The high and low doses of clove and the low dose of cinnamon improved the performance of broiler chickens at the market age in a hot tropical environment.

Estimation of malondialdehyde and catalase activity in pregnant women at IIMS&amp;R Hospital, Lucknow, India

Background: During pregnancy, the physiological production of reactive oxygen species (ROS) is associated with a variety of maternal, placental, and fetal developmental functions. These functions are disrupted by excessive amounts of ROS, resulting to pregnancy complications. Different stages of pregnancy require a balance between oxidant and antioxidant production.&#x0D; Objective: The aim of this study is to investigate the status of malondialdehyde (MDA) and catalase (CAT) activity in pregnant and age matched non-pregnant women.&#x0D; Methods: In this cross-sectional study, a total of 74 participants were enrolled, including 37 pregnant and 37 age-matched non-pregnant women. The age range of participants was 18 to 40 years. MDA and CAT levels were measured spectrophotometrically. A p-value 0.05 was statistically significant.&#x0D; Results: Compared to non-pregnant women, the mean plasma level of MDA was considerably elevated in pregnant women (p&lt;0.0001). However, the mean level of CAT activity in pregnant women was significantly lower than in non-pregnant women (p&lt;0.0001) In pregnant women, there is a significant strong negative correlation between MDA levels and CAT activity (p&lt;0.01).&#x0D; Conclusion: Pregnant women have high level of oxidative stress, indicating that the pregnant women are more susceptible to oxidative damage and may develop pregnancy-associated complications.&#x0D;

Anisodamine hydrobromide attenuates oxidative stress and proinflammatory cytokines in septic rats induced by cecal ligation and puncture.

We previously demonstrated that anisodamine hydrobromide (Ani HBr) ameliorates septic organ injury induced by lipopolysaccharide. The present study is aimed to explore the role of Ani HBr in protecting of organs against inflammation and oxidative stress in septic rats induced by cecal ligation and puncture (CLP). A total of forty-two rats were randomly divided into sham (sham operation), septic shock (CLP), Ani HBr, atropine and racemic anisodamine (Rac Ani) groups. Ani HBr (1.8, 3.6, and 5.4 mg/kg), atropine (5.4 mg/kg), and Rac Ani (5.4 mg/kg) were administrated to septic rats. After 24 h, the plasma and organs including brain, heart, liver, lung, kidney, and intestine were obtained. Then, the H&E staining and TUNEL staining were performed. The proinflammatory factors TNF-α and IL-6 and oxidative stress markers SOD and MDA in plasma were detected by ELISA. H&E staining showed that the tissues in the brain, heart, liver, lung, kidney and intestine in the septic shock group were seriously damaged. Consistently, TUNEL staining showed an increase of apoptotic cells in those tissues. Ani HBr treatment alleviated the injury and apoptotic cells in all those organs in septic rats. Ani HBr, atropine, and Rac Ani reduced the plasma TNF-α and IL-6 levels in septic rats, whereas 5.4 mg/kg Ani HBr reduced the cytokines more than Rac Ani. Ani HBr raised SOD activity and reduced plasma MDA levels in a concentration-dependent manner, which at 5.4 mg/kg were greater than atropine and Rac Ani. Therefore, anisodamine hydrobromide suppressed the proinflammatory cytokines and oxidative stress, thereby alleviating organ injury in rats with septic shock. Moreover, the therapeutic effect of Ani HBr is more powerful than that of atropine or Rac Ani, which suggests that Ani HBr is a preferred treatment for septic shock.

An array of occupationally exposure to extremely low frequency electromagnetic fields and light at night on melatonin and related oxidative stress in human subjects

During one decade, an increasing percentage of world population has to be attributed to an array of environmental, health and lifestyle factors. The potential effect of extremely low frequency electromagnetic fields (ELF-EMFs) emitted from electrical and various electrical devices in the occupational environment and light at night (LAN) to elicit biological response are a major concern for the public and scientists. Controversial genetic and oxidative stress effects have been reported by these polluted environments. Hence this study was undertaken with a purpose to evaluate melatonin hormone and oxidative stress markers in exposure workers along with cumulative effects of smoking and drinking. The study subjects (n=342) included night shift workers for more than 2 years with age, gender, socioeconomic status matched non-exposure controls (n=150). Self-reported symptoms were sought using questionnaire. Plasma Melatonin was evaluated using radioimmuno assay. Oxidative stress was estimated by measuring plasma malondialdehyde (MDA), serum nitric oxide (NO) and erythrocyte catalase levels. When the data of exposed subjects was analyzed, a significant decrease in melatonin levels was observed. Plasma MDA levels demonstrated a significant increase in one sub-group. The serum NO levels and erythrocyte catalase levels shows a significant increase. The cumulative effect of a chemical agent or environmental pollutant could be a possible contributor in enhancing the adverse effect of extremely low frequency electromagnetic fields. Certain caution is necessary.

Blood cholinesterase activities and oxidative stress status among farmworkers using pesticides in Duhok, KRG, Iraq

Background: The use of pesticides by farmworkers poses considerable health risks. This study was undertaken to examine plasma and erythrocyte cholinesterase activities, plasma oxidative biomarkers malondialdehyde (MDA), and total antioxidant status (TAS) among farmworkers using different pesticide products in Duhok, northern of Iraq. Methods: This is a case-control study conducted between November 2021 to July 2022 on 92 male farmworkers who were exposed to pesticides in comparison with 44 non-exposed male subjects (control). The availability and uses of pesticides were obtained from 19 agrochemical shops and the farmworkers exposed to pesticides. Demographic data of pesticide-exposed farmworkers and their practice of pesticide applications were recorded. Plasma and erythrocyte cholinesterase activities and plasma MDA and TAS levels were determined in both groups. Results: The farmworkers had a significant 10.0% increase in plasma MDA level, with no significant changes in blood cholinesterase activities or the TAS level. Odds and risk ratios of reduced plasma cholinesterase activity (20.0%) suggested an association of health risks in pesticide-exposed farmworkers. Most of the pesticide products (278) in use were insecticides (47.0%), which comprised mainly 26.0% pyrethroids and 3.0-7.0% anticholinesterase insecticides, among others. The majority of the farmworkers (51%) were merely aware of the general target use of the pesticide, and 75% had an exposure history of &gt; 5 years. Pesticide application was mostly (50.0%) manual, and 54.0% used insufficient personal protection equipment; 32.0% ate and drank at work, 48.0% practiced disposal of empty pesticide containers by burning and/or burying them, whereas 25.0% dumped the containers indiscriminately, and 25% disposed them at garbage sites openly. Conclusion: The farmworkers, with only a marginal increase in oxidative stress biomarker MDA, did not suffer from significant reductions in blood cholinesterase activities, although odds and risk ratios of reduced plasma cholinesterase activity suggested a health risk. Implementation of a national program is needed to measure pre-exposure blood cholinesterase activities in farmworkers.

The Role of Plasma Cell-Free Mitochondrial DNA and Nuclear DNA in Systemic Lupus Erythematosus.

The roles of plasma cell-free (pcf) mitochondrial DNA (mtDNApcf) and nuclear DNA (nDNApcf) in the pathogenesis of systemic lupus erythematosus (SLE) remain unclear. We analyzed the relative copies of mtDNApcf and nDNApcf and investigated their association with the levels of plasma 8-hydroxy-2'-deoxyguanosine (8-OHdG), plasma malondialdehyde (MDA) and mRNA of leukocyte C-type lectin domain family 5 member A (CLEC5A) in SLE patients. A total of 80 SLE patients and 43 healthy controls (HCs) were enrolled. Their plasma samples were subjected to the measurements of mtDNApcf copies, nDNApcf copies, 8-OHdG and MDA, respectively. Their leukocytes were analyzed for CLEC5A mRNA expression. SLE patients had higher nDNApcf copies (2.84 ± 1.99 vs. 2.00 ± 0.88, p = 0.002), lower mtDNApcf copies (4.81 ± 6.33 vs. 9.83 ± 14.20, p = 0.032), higher plasma 8-OHdG (0.227 ± 0.085 vs. 0.199 ± 0.041 ng/mL, p = 0.016), lower plasma MDA (3.02 ± 2.20 vs. 4.37 ± 2.16 μM, p = 0.001) and similar leukocyte CLEC5A mRNA expression levels (1.21 ± 1.17 vs. 1.26 ± 1.05, p = 0.870), as compared with those of HCs. Among the HCs, SLE patients with SLE Disease Activity Index (SLEDAI) ≤8, and SLE patients with SLEDAI >8, their respective mtDNApcf copies decreased stepwisely (9.83 ± 14.20 vs. 6.28 ± 7.91 vs. 3.19 ± 3.35, p = 0.054). The nDNApcf copies of HCs, SLE patients without nephritis, and SLE patients with nephritis were increased stepwisely (2.00 ± 0.88 vs. 2.63 ± 1.74 vs. 3.16 ± 2.34, p = 0.043). Among SLE patients, higher nDNApcf copies were associated with higher levels of plasma 8-OHdG (p < 0.001) but lower plasma MDA (p = 0.019). Among HCs but not SLE patients, higher nDNApcf copies (p = 0.013) or lower mtDNApcf copies (p < 0.001) were related to higher levels of leukocyte CLEC5A mRNA expression. Higher nDNApcf, lower mtDNApcf, increased ROS-elicited oxidative DNA damage and dysregulated leukocyte CLEC5A expression might be implicated in the pathogenesis of SLE.

Effect of 4 Weeks HIIT with Spirulina Supplementation Intake on Plasma Total Antioxidant Capacity (TAC) and Lipid Peroxidation (MDA) in Women with Type 2 Diabetes

Objective: Oxidative stress plays a key role in the pathogenesis of type 2 diabetes mellitus (T2DM) and its complications. Exercise and anti-oxidant supplements are two potential approaches to delay the development of T2DM. The purpose of this study was to evaluate the interaction effects of spirulina supplementation and high intensity interval training (HIIT) on oxidative stress and total antioxidant capacity in inactive women with T2DM.&#x0D; Materials and Methods: This research was a quasi-experimental study with pretest-posttest control group design. Our study subjects were 55 women with T2DM (age of 51.95 ± 5.57 years and BMI of 30.55 ± 4.63 kg/m2 ) that were randomly divided into 4 groups: 1- exercise and spirulina (n= 15), 2- spirulina (n= 15), 3- placebo (n= 15), 4-control (n= 10) without exercise and supplementation. Participants received 2 grams spirulina supplement per day. Training program included three sessions pre-week walking and running on a treadmill for 4 weeks, each session consisted of 10 minutes of warming and 10 minutes of cooling with a 50- 70% HRR intensity and 25 minutes of HIIT (The training interval of 4-minute sections with 85-95 % HRR intensity and 3-minute active rest sections, with 50-70 % HRR intensity). All evaluations were performed with SPSS statistical software using analysis of covariance to assess between-group differences and t-test to assess within-group differences.&#x0D; Results: Our study results showed that the plasma level of MDA decreased significantly in the exercise + placebo group compared to the control group (P= 0.03). However, the level of TAC was not changed significantly in our experimental groups compared to the control group (P= 0.7).&#x0D; Conclusion: Based on the findings of this study the spirulina supplementation and HIIT can be good stimuli for reducing oxidative stress in women with T2DM.

Slow flow induces endothelial dysfunction by regulating thioredoxin-interacting protein-mediated oxidative metabolism and vascular inflammation.

Endothelial cells are highly sensitive to hemodynamic shear stresses, which act in the blood flow's direction on the blood vessel's luminal surface. Thus, endothelial cells on that surface are exposed to various physiological and pathological stimuli, such as disturbed flow-induced shear stress, which may exert effects on adaptive vascular diameter or structural wall remodeling. Here we showed that plasma thioredoxin-interactive protein (TXNIP) and malondialdehyde levels were significantly increased in patients with slow coronary flow. In addition, human endothelial cells exposed to disturbed flow exhibited increased levels of TXNIP in vitro. On the other hand, deletion of human endothelial TXNIP increased capillary formation, nitric oxide production and mitochondrial function, as well as lessened oxidative stress response and endothelial cell inflammation. Additional beneficial impacts from TXNIP deletion were also seen in a glucose utilization study, as reflected by augmented glucose uptake, lactate secretion and extracellular acidification rate. Taken together, our results suggested that TXNIP is a key component involved in mediating shear stress-induced inflammation, energy homeostasis, and glucose utilization, and that TXNIP may serve as a potentially novel endothelial dysfunction regulator.

Preliminary Effects of Osteopathic Manipulative Medicine on Reactive Oxygen Species in Parkinson's Disease: A Randomized Controlled Pilot Study.

Context Parkinson's disease (PD) is the second most common neurodegenerative disorder and causes many clinical manifestations including bradykinesia, tremor, postural instability, and musculoskeletal stiffness. Neurodegeneration is commonly associated with oxidative stress. Oxidative stress has not been measured in PD in relation to the manual techniques used in Osteopathic Manipulative Treatment (OMT). Objective To investigate the effect of OMT on oxidative stress biomarkers in PD. Methods In this randomized non-blinded study, 32 PD subjects were separated by block randomization into counseling and OMT groups, receiving respective interventions twice a week for six weeks. The counseling arm received informative sessions while the OMT arm received a set treatment protocol. Biomarkers of oxidative stress, malondialdehyde (MDA), dityrosine (DT), 3-nitrotyrosine (3-NT), 8-hydroxy-2-deoxyguanosine (8-OHdG), and 8-isoprostane were measured before and after the first session and at weeks three, six, and 10 (four weeks after conclusion of intervention). Results No significant changes were found in blood plasma levels of MDA, DT, 3-NT, or 8-OHdG during or after intervention compared to controls (p > 0.05). No significant changes were found in urine 8-OHdG or 8-isoprostane during or after intervention compared to controls (p > 0.05). Conclusion OMT used in this study did not significantly affect the chosen oxidative stress biomarkers, however many limitations of the study may have impeded possible findings.

Curcumin Supplementation in Semen Extender Improves Post-Thaw Quality and Antioxidant Capacity of Gir Bull Spermatozoa

This investigation was carried out on semen of three Gir bulls at Cattle Breeding Farm, Junagadh (India) for a period of 6 months. The aim was to assess the effect of different concentrations of Curcumin, viz., 0 µM (Control), 5 µM, 10 µM, and 15 µM in Andromed extender on sperm quality along with oxidative stress parameters of cryopreserved semen. Semen ejaculates (n=30, 10/bull) with &gt;70% initial motility were included in the study. Soon after initial evaluation, the semen was extended in Andromed extender @ 80 million sperm per ml and was divided into four equal aliquots. The Curcumin stock solution (10 mM) was added in these aliquots to get a final concentration of 0, 5, 10, and 15 µM and sperm motility was checked. It was soon filled and sealed in medium French straws (0.5 ml), equilibrated at 4°C for 4 h and frozen in LN2 vapour using conventional wide mouth freezer. Thawing of straws was done next day in water bath at 37°C for 30 seconds, and various sperm quality parameters including oxidative markers were assessed. The results showed that supplementation of Curcumin to freezing extender improved semen quality and antioxidant capacity. The post-thaw sperm motility, sperm viability, HOST reactive sperm and acrosomal integrity in semen extended with 15 µM Curcumin concentration (57.33±0.67, 73.20±1.05, 64.77±1.56 and 72.43±1.38 %, respectively) were significantly (p&lt;0.05) higher and sperm abnormality (18.00±0.41%) was significantly (p&lt;0.05) lower as compared to those of control extender and the 5 µM Curcumin group. Mean malondialdehyde (MDA) level in post-thaw seminal plasma did not differ significantly (p&gt;0.05) among the groups, while total antioxidants activity (TAC) with 15 µM Curcumin (476.36±16.52 μmol/L) was significantly (p&lt;0.05) higher as compared to control and 5 µM Curcumin group. It is concluded that Curcumin @ 15 µM in semen extender improves post-thaw sperm quality and antioxidant capacity of cryopreserved Gir bull semen.

Toxicological Outcome of Combined Exposure to Potassium Dichromate and Levonorgestrel in the Kidney of Female Rats

Co-exposure to chromate (VI) compound and oral contraceptives is common in our environment especially among women working in chromate-related industries. Exposure to either chromate (VI) or oral contraceptives is linked with the etiology of several diseases including cancers and renal injury. However, there is paucity of information on the toxic effect of combined co-exposure to both compounds. The present study examines the toxicity of combined exposure to potassium dichromate (PDC) and an oral contraceptive, levonorgestrel in the kidney of female rats. Control animals were fed distilled water, while experimental rats were injected 12 mg/kg body weight of PDC once a week for six weeks and oral daily exposure to 15µg/kg body weight of levonorgestrel either alone or in combination. Absolute and relative kidney weight, renal function, oxidative stress and pathological lesion were assessed in plasma and kidney of control and experimental rats. The PDC and levonorgestrel significantly (p&lt;0.05) increased plasma urea creatinine and malondialdehyde levels in treated-rats, while renal superoxide dismutase and glutathione-S-transferase activities were reduced by both compounds. Moreover, histopathological lesions including necrotizing nephritis was observed in the kidney of PDC-treated rats, while tubular epithelial degeneration and necrosis was observed in levonorgestrel-treated rats. Combined exposure to both compounds aggravated the increase in urea, creatinine and renal damage. Additionally, the antioxidant enzymes were further repressed in the co-treatment group. The study suggests that combined exposure to potassium dichromate and levonorgestrel worsened nephrotoxicity in rats by increasing oxidative stress.

The Efficacy of Therapeutic-Diabetes Mellitus Functional Drink on Blood Glucose and Plasma Malondialdehyde (MDA) Levels of Type 2 Diabetes Mellitus Patients

A long-time condition of hyperglycemia in type 2 diabetes mellitus (DM) will cause glucose auto-oxidation, which can increase reactive oxygen stress. Improving the balance between the composition of gut microbiota and host cells in DM patients with prebiotics and probiotics concepts is a therapy to reduce the risk of ongoing inflammation. This study aimed to examine the efficacy of the therapeutic diabetes mellitus functional beverage product from local functional food on blood glucose levels and plasma malondialdehyde (MDA) levels of type 2 DM patients. This study used a “pre-posttest control design”. The research subjects were 46 patients with type 2 DM who were divided into two groups: the intervention and control groups. The research sample was selected by a purposive sampling technique. The intervention was conducted by giving 200 ml of yogurt bengkuang tape ketan hitam for two weeks. Meawhile, the statistical test used an independent t-test. The results showed that the samples did not have a difference in the mean blood glucose levels before and after the Yobetam treatment. The blood glucose levels of the control group were 4.9 ± 39.3, and those of the intervention group were -14.1 ± 52.1. However, the samples showed a difference in the mean MDA levels before and after the Yobetam treatment. The mean MDA levels of the control group were 0.16 ± 0.39, and those of the intervention group were 0.46 ± 0.37. The functional drink is expected to be an alternative to oral therapy.

The Effect of Dietary Inclusion of Microalgae Schizochytrium spp. on Ewes' Milk Quality and Oxidative Status.

An unprecedented challenge for nutritionists arises during the 21st century in order to produce highly nutritious and functional food which promotes human health. Polyunsaturated fatty acids (PUFA) that are highly contained in microalgae have broadly been confirmed for preventing cardiovascular diseases and regulating immune-oxidative status. However, their optimum dietary inclusion level needs to be defined since PUFA are prone to oxidation. For this purpose, 24 cross-bred dairy ewes, were separated into four groups (n = 6) and were fed with different levels of microalgae Schizochytrium spp. [0 (CON, no microalgae), 20 (SC20), 30 (SC30) and 40 (SC40) g/ewe/day] for 60 days. The results showed that although the production parameters were not impaired, milk fat content was decreased in medium and high-level supplemented groups while protein content was suppressed only for the medium one. Concerning the fatty acids (FA) profile, the proportions of C14:0, trans C18:1, trans-11 C18:1, cis-9, trans-11 C18:2, trans-10, cis-12 C18:2, C20:5 (EPA), C22:5n-6 (DPA), C22:6n-3 (DHA), the total ω3 FA and PUFA were significantly increased, while those of C18:0, cis-9 C18:1 and C18:2n-6c were decreased in the milk of treated ewes. Additionally, in the S40 group an oxidative response was induced, observed by the increased malondialdehyde (MDA) levels in milk and blood plasma. In conclusion, the dietary inclusion of 20 g Schizochytrium spp./ewe/day, improves milks’ fatty acid profile and seems to be a promising way for producing ω3 fatty acid-enriched dairy products.

THE EFFECTIVENESS OF KAFFIR LIME PEEL EXTRACT (Citrus hystrix) ON PLASMA MALONDIALDEHYDE LEVEL IN DEMENTIA MICE

ABSTRACTBackground: Antioxidant is a substance that can catch and neutralize free radicals so that the process of oxidative stress can be stopped and cell damage can be avoided. Kaffir lime peel contains antioxidant compounds that have the potential to be the neuroprotective agent and can protect neurons from free radical-induced damage.Objective: To evaluate the effect of kaffir lime peel extract (Citrus hystrix) on the plasma malondialdehyde (MDA) level of mice induced by Scopolamine (SCM).Method: This research was a true experimental study with a post-test-only controlled group design. Thirty mice were randomly divided into 5 groups consisting of a healthy control (K+), Negative Control (K-), extract 5 mg/20gBW group (P1), 10 mg/20gBW group (P2), 20 mg/20gBW group (P3). SCM was injected intraperitoneally on days 1-7 and kaffir lime peel extract was given orally on days 2-7, the MDA levels testing of mice using TBARS method on day 8. Data analysis used One-Way ANOVA test and continued with Post Hoc LSD test.Results: The mean MDA levels of K+, K-, P1, P2, P3 were 4,212; 4,644; 3,481; 4,555; 4,733 nmol/mL, respectively. MDA levels of P1 were lower than K-, even though there were no statistically significant differences.Conclusion: There was no effect of administration kaffir lime peel extract (Citrus hystrix) on MDA levels in mice with scopolamine-induced dementia.Keywords: Citrus hystrix, malondialdehyde, dementia, mice

Dietary fructooligosaccharide and Bacillus licheniformis on growth performance, digestive enzyme, immune indices, and antioxidant capacity of common carp (Cyprinus carpio)

A feeding trial of eight weeks was conducted to investigate the effects of dietary fructooligosaccharide (FOS), Bacillus licheniformis (B. licheniformis) and synbiotic in common carp (Cyprinus carpio). 360 fish averaging 12.5 ± 0.5 g (mean ± SD) were randomly divided into four groups in triplicate. D1 was the control group, feeding the basal diet. Diet 2, 3, 4 (D2, D3, D4) were test groups, adding 0.3% FOS, 1 × 107cfu/g B.licheniformis, and 0.3% FOS with 1 × 107cfu/g B.licheniformis to the basal diets (33.16% crude protein, 6.52% crude lipid and 4.93% ash), respectively. The results showed that the growth parameters such as weight gain, specific growth rate and protein efficiency rate were significantly higher in D4 group compared with the control group. The Feed conversion ratio in D2 and D4 groups had lower values compared to the control group. The intestinal digestive enzymes including protease and lipase were significantly higher in D4 group than that of the control group. On the other hand, aspartate aminotransferase and alanine aminotransferase activities, triglyceride and cholesterol content in the D4 group were significantly decreased compared with D1 group. Furthermore, lysozyme, acid phosphatase activities, and plasma complements (C3 and C4) levels in fish fed D3 and D4 diets were significantly higher than the control group. Additionally, the antioxidant status was improved, that coincided with an increase in superoxide dismutase and catalase activities, Total antioxidative capacity as well as a decrease in malondialdehyde levels in plasma. Overall, these results indicated that dietary supplementation of either individually or in combination (synbiotic) could improve growth, the immune indices, and antioxidant function of Cyprinus carpio. The combinational group showed the better result.

Maternal resveratrol regulates the growth performance, antioxidant capacity, and intestinal health of suckling piglets through intestinal microorganisms at high summer temperatures.

BackgroundResveratrol has numerous beneficial properties, including antioxidant, anti-inflammatory, and immunomodulatory properties. High summer temperatures in Southern China affect the reproductive performance of sows. The present study aimed to investigate the effects of dietary resveratrol supplementation in different thermal environments on the reproductive performance, antioxidant capacity, immune function, and intestinal microbes of sows and piglets during late gestation and lactation, as well as their relationship with colostrum immunoglobulin.MethodsA two-phase experiment was conducted with 40 healthy multiparous sows. In the first phase of the experiment, 20 sows were used in a moderate temperature environment, and in the second phase of the experiment, the remaining 20 sows were used in a high-temperature environment. In both phases, sows were fed either a control diet or a diet consists of control diet and 300 mg/kg resveratrol starting on day 75 of gestation. Plasma, milk, and fecal samples were collected to obtain the indices of antioxidant capacity, immune function, and intestinal microbes.ResultsThe results showed that resveratrol supplementation increased the number of live births by 13.24 and 26.79% in the first and second phases, respectively, compared with the control group. In the second phase, resveratrol supplementation increased litter weight at weaning and in the concentrations of growth hormone (GH), insulin (INS), progesterone (PROG), triglycerides, and uric acid (UA). The plasma superoxide dismutase (SOD) level on day 110 of gestation and day 14 of lactation, as well as glutathione peroxidase (GSH-Px) on day 14 of lactation in the first phase, showed an increasing trend (p = 0.0728, p = 0.0932, and p = 0.067, respectively) in the resveratrol group, compared with the control group. On day 14 of lactation, the plasma total antioxidant capability (T-AOC) level was higher in the second phase, while the plasma malondialdehyde (MDA) level was lower in both phases in the resveratrol group. Resveratrol supplementation increased the abundance of immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) in colostrum and the relative abundance of Lactobacillus and Alloprevotella but decreased the relative abundance of Escherichia-shigella in piglet feces in the second phase. In addition, Spearman's correlation analysis indicated that the weight gain of weaned piglets was positively (p < 0.05) associated with IgM content in colostrum and the abundance of Lactobacillus in the fecal microbiota of piglets in the second phase. Moreover, the abundance of Alloprevotella was positively correlated with the contents of IgA and IgG in colostrum, while the abundance of Lactobacillus was positively correlated with IgM content.ConclusionThese findings indicated that maternal resveratrol supplementation could enhance the growth performance, antioxidant capacity, and intestinal health of piglets in a high temperature environment, which might be associated with increased immunoglobin secretion from colostrum.

Effect of Resveratrol Supplementation on Intestinal Oxidative Stress, Immunity and Gut Microbiota in Weaned Piglets Challenged with Deoxynivalenol.

(1) Background: Deoxynivalenol (DON) is a general mycotoxin that induces severe intestinal barrier injury in humans and animals. Resveratrol (RES) efficiently exerts anti-inflammatory and antioxidant effects. However, the information regarding RES protecting against DON-induced oxidative stress and intestinal inflammation in piglets is limited. (2) Methods: A total of 64 weaned piglets (Duroc × (Landrace × Yorkshire), 21-d-old, barrow) were randomly allocated to four groups (eight replicate pens per group, each pen containing two piglets) for 28 d. The piglets were fed a control diet (CON) or the CON diet supplemented with 300 mg RES/kg diet (RES group), 3.8 mg DON/kg diet (DON) or both (DON+RES) in a 2 × 2 factorial design. (3) Compared with unsupplemented DON-challenged piglets, RES supplementation in DON-challenged piglets increased ileal villus height and the abundance of ileal SOD1, GCLC and PG1-5 transcripts and Muc2 protein (p < 0.05), while decreasing the mRNA and proteins expression of ileal IL-1β, IL-6 and TNF-α, and malondialdehyde (MDA) levels in plasma and ileum in DON-challenged piglets (p < 0.05). Moreover, the abundances of class Bacilli, order Lactobacillales, family Lactobacillaceae and species Lactobacillus gasseri were increased in DON-challenged piglets fed a RES-supplemented diet compared with those in DON-challenged piglets(p ≤ 0.05). (4) Conclusions: our results indicated that RES supplementation in DON-challenged piglets efficiently attenuated intestinal inflammation and oxidative stress and improved gut microbiota, thereby alleviating DON-induced intestinal barrier injury.

Exercise Training Ameliorates Renal Oxidative Stress in Rats with Chronic Renal Failure.

In patients with chronic kidney disease, exercise training with moderate intensity protects renal function and improves mortality. However, the mechanisms of the renal protective effects of exercise training in chronic kidney disease have not been clarified. This study investigated the effects of exercise training on renal NADPH oxidative and xanthine oxidase, which are major sources of reactive oxygen species, in rats with chronic renal failure. Six-week-old, male Sprague–Dawley rats were divided into the sham operation, 5/6 nephrectomy (Nx)+ sedentary, and Nx+ exercise training groups. The Nx+ exercise training group underwent treadmill running. After 12 weeks, systolic blood pressure, renal function, malondialdehyde, renal NADPH oxidase, and xanthine oxidase were examined. Nx induced hypertension, proteinuria, and renal dysfunction, and exercise training attenuated these disorders. Although the plasma levels of malondialdehyde were not different among the group, urinary levels were increased by Nx and decreased by exercise training. Renal activity and expression of NADPH oxidase and xanthine oxidase were increased by Nx and decreased by exercise training. These results indicate that exercise training attenuates hypertension and renal dysfunction and ameliorates NADPH oxidase and xanthine oxidase in rats with chronic renal failure, suggesting that the reduction of reactive oxygen species generation may be involved in the renal protective effects of exercise training.

Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration.

Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed to determine relative telomere length (RTL), oxidative stress status, and antioxidant levels and examine the relationships between RTL, oxidative stress, and the severity in LDD patients. A total of 100 subjects, 50 LDD patients and 50 healthy controls, were enrolled in the case–control study. Blood leukocyte RTL was analyzed using quantitative real-time polymerase chain reaction. Lipid peroxidation was determined by malondialdehyde (MDA) assay. Plasma 8-hydroxy 2′-deoxyguanosine (8-OHdG) values were determined using enzyme-linked immunosorbent assay. Total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) in plasma were also measured. The LDD patients had significantly shorter telomeres than the healthy controls (p = 0.04). Blood leukocyte RTL was inversely correlated with the LDD severity (r = −0.41, p = 0.005). Additionally, plasma MDA and 8-OHdG levels were markedly greater in LDD patients than in the controls (p = 0.01 and p = 0.002, respectively). Furthermore, the plasma MDA level showed a positive correlation with the radiographic severity (r = 0.49, p = 0.001). There was a positive correlation between plasma 8-OHdG and the severity (r = 0.60, p < 0.001). Moreover, plasma TAC and FRAP levels were significantly lower in LDD patients than in the controls (p = 0.04). No significant differences in plasma TAC and FRAP were observed among the three groups of LDD severity. We found that RTL was negatively correlated with the severity while plasma MDA and 8-OHdG levels were positively correlated with the severity. These findings suggest that blood leukocyte RTL, plasma MDA, and 8-OHdG may have potential as noninvasive biomarkers for the assessment of severity in LDD.

Arabic Gum Could Alleviate the Aflatoxin B1-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways.

Aflatoxin B1 (AF) is an unavoidable environmental pollutant that contaminates food, feed, and grains, which seriously threatens human and animal health. Arabic gum (AG) has recently evoked much attention owing to its promising therapeutic potential. Thus, the current study was conducted to look into the possible mechanisms beyond the ameliorative activity of AG against AF-inflicted hepatic injury. Male Wistar rats were assigned into four groups: Control, AG (7.5 g/kg b.w/day, orally), AF (200 µg/kg b.w), and AG plus AF group. AF induced marked liver damage expounded by considerable changes in biochemical profile and histological architecture. The oxidative stress stimulated by AF boosted the production of plasma malondialdehyde (MDA) level along with decreases in the total antioxidant capacity (TAC) level and glutathione peroxidase (GPx) activity. Additionally, AF exposure was associated with down-regulation of the nuclear factor erythroid2–related factor2 (Nrf2) and superoxide dismutase1 (SOD1) protein expression in liver tissue. Apoptotic cascade has also been evoked following AF-exposure, as depicted in overexpression of cytochrome c (Cyto c), cleaved Caspase3 (Cl. Casp3), along with enhanced up-regulation of inflammatory mediators such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, inducible nitric oxide synthase (iNOS), and nuclear factor kappa-B transcription factor/p65 (NF-κB/p65) mRNA expression levels. Interestingly, the antioxidant and anti-inflammatory contents of AG may reverse the induced oxidative damage, inflammation, and apoptosis in AF-exposed animals.