Plasma Antioxidant Parameters Research Articles

Antioxidant status in the blood, liver, and muscle tissue of turkey hens receiving a diet with alfalfa protein concentrate

This study aimed to investigate the impact of the oxidative potential of turkeys fed a diet with alfalfa protein concentrate (APC), used throughout the rearing period or periodically at 2-wk intervals. The research material consisted of 6-wk-old BIG 6 turkey hens kept in pens, 5 birds per pen in 6 replicates. The experimental factor was the addition of APC to the diet in the amount of 15 or 30 g/kg of diet. APC was administered in 2 ways: birds received a diet with APC throughout the experiment or periodically. In the latter case, the birds received the diet with APC for 2 wk, and then for 2 wk they received the standard diet without APC. Levels of nutrients in the diet; flavonoids, polyphenols, tannins, and saponins in APC; uric acid, creatinine, bilirubin, and some antioxidants in the blood; and enzyme parameters in the blood and tissues of turkeys were determined. The use of APC in the diet stimulated antioxidant processes, which could be seen in the values of the pro-oxidant-antioxidant parameters of the tissues and blood plasma of turkeys. The significant reduction in the H2O2 level (P = 0.042) and slight reduction in the MDA level (P = 0.083), accompanied by an increase in catalase (P = 0.046) activity in the turkeys continuously receiving APC in the amount of 30 g/kg of diet, as well as the increase in plasma antioxidant parameters (vitamin C, P = 0.042 and FRAP, P = 0.048) in these birds, reflects improvement in their antioxidant status. Thus continuous use of the APC supplement in the amount of 30 g/kg of diet proved to be a better feeding practice to optimize oxidative potential than periodic inclusion of APC.

Maternal Dietary Supplementation with γ-Aminobutyric Acid Alleviated Oxidative Stress in Gestating Sows and Their Offspring by Regulating GABRP.

Simple SummarySows usually suffer oxidative stress during gestation, and this limits the growth of fetuses via placenta. Gamma-aminobutyric acid (GABA) is a functional nonessential amino acid engaged in regulating the physiological status of animals. However, the effects of GABA on the oxidative homeostasis of sows and their offspring remain unclear. This study aims to investigate the effects of GABA on the oxidative stress of gestating sows, newborn piglets, as well as the H2O2-treated porcine trophectoderm cell line-2 (pTr-2 cells) and to subsequently provide guidance on the application of GABA in sows’ nutrition during late gestation. We found that GABA alleviated placental oxidative stress in gestating sows by increasing antioxidant-associated enzyme activity and by upregulating the gene expressions of gamma-aminobutyric acid receptor (GABRP) and nuclear factor-erythroid 2-related factor-2 (Nrf2) in pTr-2 cells, as well as enhanced the antioxidant capacity of sows and fetal piglets. This study provides the possibility for future application of GABA in maternal–fetal nutrition.Sows usually suffer oxidative stress during gestation, and this limits the growth of fetuses via placenta. Gamma-aminobutyric acid (GABA) is a functional nonessential amino acid engaged in regulating the physiological status of animals. However, the effects of GABA on the oxidative homeostasis of sows and their offspring remain unclear. Eighteen late gestating sows (85 d) were divided into the CON and GABA groups and fed the basal diet and the GABA diet (200 mg/kg GABA), respectively, until farrowing. At parturition, the sows’ litter characteristics, the plasma antioxidant parameters of sows, and their offspring were evaluated. The results showed that GABA supplementation had no marked effect on the reproductive performance of sows (p > 0.10) but had a trend of reducing the amount of intrauterine growth restriction (IUGR) in piglets (0.05 < p < 0.10). At the same time, the addition of GABA elevated the plasma superoxide dismutase (SOD) level of sows and enhanced the glutathione peroxidase (GSH-Px) activity of newborn piglets (p < 0.05). Based on the H2O2-induced oxidative stress in pTr-2 cells, GABA elevated intracellular GSH-Px, SOD, catalase (CAT), and total antioxidant capacity (T-AOC, p < 0.01) and upregulated the gene expressions of CAT, gamma-aminobutyric acid receptor (GABRP), and nuclear factor-erythroid 2-related factor-2 (Nrf2) in H2O2-treated pTr-2 cells (p < 0.05). Taken together, GABA improved the antioxidant capacity of sows and alleviated the placental oxidative stress by upregulating the GABRP and Nrf2 genes, which have the potential to promote oxidative homeostasis in newborn piglets.

Effects of partial replacement of fish meal with porcine meat meal on growth performance, antioxidant status, intestinal morphology, gut microflora and immune response of juvenile golden pompano (Trachinotus ovatus)

This study was conducted to investigate the effects of partial replacement of fish meal (FM) with porcine meat meal (PMM) on growth performance, plasma antioxidant parameters, intestine morphological parameters, gut microflora and expression of intestinal Nrf2 and Nf-κB signaling pathway of juvenile golden pompano. Fish were fed with six experimental diets (0 (PC), 10% (P10), 20% (P20), 30% (P30), 40% (P40) and 50% (P50) replacement levels) for 8-weeks. The percent weight gain and specific growth rate in fish fed with P40 and P50 diets were significantly lower than those of the control group (P < 0.05). Compared with the control group, the plasma antioxidant indexes including the activities of superoxide dismutase, catalase, glutathione peroxidase, total antioxidant capacity and malondialdehyde content in fish fed with P30, P40 and P50 diets were significantly affected (P < 0.05). With dietary replacement of FM with PMM increasing 0 to 30%, the relative expressions of IL-10, TGF-β, Nrf2, SOD, Cu/Zn-SOD and GSH-Px were significantly down-regulated, while the relative expressions of NF-κB, TNF-α, IL-1β and IL-8 were significantly up-regulated (P < 0.05). Villus heights and crypt depths of proximal and mid intestine were significantly affected with dietary replacement of FM with PMM increasing 0 to 40% (P < 0.05). The structure of the intestinal microbial flora showed that the relative abundances of beneficial bacteria decreased, and the relative abundances of pathogenic bacteria increased in fish fed with P50 diet compared with the control group. In conclusion, replacing FM with PMM up to 30% had no significant impact on growth performance and feed utilization of golden pompano. However, 30% replacements of FM with PMM exerted adverse effects on antioxidant capacity and immune response of fish. In addition, replacement of a high proportion of FM with PMM negatively affect the gut microflora of fish.

Effects of aromatic plant extract mixture on laying efficiency, egg quality and antioxidant status in laying quails

This study aimed to evaluate the effects of commercial plant extract mixture added to laying quail rations on performance, egg quality, plasma antioxidant and blood biochemical parameters. In this experiment, 160 Japanese quails aged twelve weeks were equally divided into four groups of 40 (five replicates of 8 quails each). The study included a control and three treatment groups. The treatment groups were as follows: (1) 100 mg/kg aromatic plant extract mixture-group 1; (2) 150 mg/kg aromatic plant extract mixture-group 2 (3) 200 mg/kg aromatic plant extract mixture-group 3, respectively. When examined the egg parameters at the end of the study, significant differences (P&amp;lt;0.05) were found in the parameters of egg weight, Haugh unit (HU), albumen index, egg yolk color and eggshell thickness. Egg weight and eggshell thickness values were measured the highest (P&amp;lt;0.05) in group 1. HU, egg yellow color and albumen index values were found significantly higher (P&amp;lt;0.05) in all experimental groups compared to the control group. Significant differences (P&amp;lt;0.05) were determined in superoxide dismutase (SOD) and malondialdehyde (MDA) values of plasma antioxidant parameters in the study. In the study, High density lipoprotein (HDL) value, one of the blood (plasma) parameters, was found to be higher (P&amp;lt; 0.001) in the experimental groups. Consequently, we can say that the addition of herbal extract mixture to quail diets increases egg quality and has a positive effect on cholesterol and plasma antioxidant parameters

The Effect of Thaumatococcus danielli Leaf Extracts on Immunological and Oxidative Stress Markers in Rat

This work demonstrated the immunological and antioxidant activities of Thaumatococcus daniellii leaf extracts and their potential as the pharmaceutical factor for the treatment of some pathological disorders such as cancer, leucopenia, cells inflammation, dietary, atherosclerosis, neurodegenerative disease and other immunological and oxidative stress disorders.36 albino rats of average weight 140 ± 5 g were randomly grouped into 6 with six rats per group. The rats in group A (positive control) received only 0.1% DMSO. The rats in the negative control (group B) received pyrogallol (immunosuppressor) + 0.1% DMSO for 7 days and challenged with 0.1ml Sheep Red Blood Cell (SRBC). The test groups (C to F) also received pyrogallol + 0.1% DMSO for 7 days and were also challenged with SRBC for a period of 72hours after which they were treated with High and Low doses of either petroleum ether or water extracts of Thaumatococcus danielii for 21days. The assessment of immunomodulatory activity was carried out using Carbon Clearance Test. Haematological/ immunological parameters, Spleen and Plasma antioxidant parameters like Catalase (CAT), Superoxide dismutase (SOD), Glutathione Peroxidase (GPX) Activities, Lipid Peroxidation (LPO) and Malonaldehyde (MDA) concentrations were also determined. Result unveiled a significant (p&lt;0.05) increase in the phagocytic index of both high doses of water and petroleum ether extracts compared to the negative control group. There were no significant (p&lt;0.05) changes in the Heamatological parameters and plasma catalase, but there was a significant change in the white blood cell (WBC) count and also an improvement in the spleen and plasma antioxidant parameters. Further study is required to understand the mechanism of action of both extracts in order to exploit it in immunomodulation.

The immunoreaction and antioxidant capacity of juvenile blunt snout bream (Megalobrama amblycephala) involves the PI3K/Akt/Nrf2 and NF-κB signal pathways in response to dietary methionine levels

A 75-day rearing trail was designed to evaluate the immunoreaction and antioxidant capacity of juvenile blunt snout bream in response to dietary methionine levels. Three practical diets were extruded to feed juveniles with graded methionine levels (0.40%, 0.84% and 1.28% dry matter). The data indicated that the plasma concentrations of immunoglobulin M (IgM), complement component 3 (C3) and glutathione (GSH) in the 0.84% methionine diet were markedly upper than those in the 0.40% group (P < 0.05). The activities of plasma antioxidant parameters involving catalase (CAT), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) and glutathione peroxidase (GPx) were significantly increased by the 0.84% diet compared with the 0.40% diet, whereas plasma alanine aminotransferase (ALT) and malondialdehyde (MDA) levels were significantly induced by 0.40% methionine (P < 0.05). Compared with the 0.40% group, 0.84% dietary methionine dramatically upregulated the mRNA expression levels of protein kinase B (Akt), phosphoinositide 3-kinase (PI3K) and nuclear factor erythroid 2-related factor 2 (Nrf2) pathway related genes including CAT, manganese superoxide dismutase (Mn-SOD), heme oxygenase 1 (HO-1) and glutathione peroxidase-1 (GPx-1) in the kidney and liver, and downregulated Kelch-like ECH-associated protein 1 (Keap1) mRNA levels (P < 0.05). Compared with the 0.40% group, the 0.84% dietary methionine strikingly suppressed the mRNA levels of renal and hepatic nuclear factor-kappa B (NF-κB) and pro-inflammatory cytokines (interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6)), however, improved the mRNA expression levels of anti-inflammatory cytokines involved renal and hepatic transforming growth factor-β (TGF-β) and hepatic interleukin 10 (IL-10) (P < 0.05). Renal IL-10 and interleukin 8 (IL-8) mRNA expression levels were not markedly influenced by experimental diets (P > 0.05). Dietary methionine (0.84%) significantly upregulated renal and hepatic heat stress protein 70 (Hsp70), renal B-cell lymphoma-2 (Bcl-2) gene expression levels compared with the 0.40% diet (P < 0.05). In a word, the data represented that 0.84% dietary methionine could enhance the immune and antioxidant capacity of this fish species by inducing PI3K/Akt/Nrf2 pathway and inhibiting NF-κB pathway.

Dietary Vitamin C, E and β-Carotene Intake Does Not Significantly Affect Plasma or Salivary Antioxidant Indices and Salivary C-Reactive Protein in Older Subjects.

It is not clear whether habitual dietary intake influences the antioxidant or inflammatory status. The aim of the present study was to assess the impact of antioxidative vitamins C, E, and β-carotene obtained from daily food rations on plasma and salivary Total Antioxidant Capacity (TAC), uric acid and salivary C-reactive protein (CRP). The study involved 80 older subjects (66.9 ± 4.3 years), divided into two groups: group 1 (n = 43) with lower and group 2 (n = 37) with higher combined vitamins C, E and β-carotene intake. A 24-h dietary recall was obtained from each individual. TAC was assessed simultaneously with two methods in plasma (Ferric Reducing Ability of Plasma—FRAP, 2.2-diphenyl-1-picryl-hydrazyl—DPPH) and in saliva (FRAS and DPPHS test). Lower vitamin C intake corresponded to higher FRAS. There were no other correlations between vitamins C, E or β-carotene intake and antioxidant indices. Salivary CRP was not related to any antioxidant indices. FRAS was decreased in group 2 (p < 0.01) but no other group differences for salivary or for plasma antioxidant parameters and salivary CRP were found. Habitual, not extra supplemented dietary intake does not significantly affect plasma or salivary TAC and salivary CRP.

Whole body vibration as a safe exercise training method induces no impaired alterations on rat plasma antioxidant biomarkers

Whole body vibration (WBV) has been regarded as an exercise training method and as a non-pharmacological supportive treatment option appearing to be efficient in chronic disease conditions, such as bone disorders and for cardio-respiratory fitness. Since, data on the safety and efficacy of vibration on oxidative stress parameters are lacking, it was decided to assess the effects of WBV on the plasma antioxidant biomarkers in adult male Wistar rat model. Male Wistar rats weighing 140-180 g, were divided into control and vibration group. Vibration training consisted of vertical sinusoidal whole body vibration for 8 weeks, followed by blood collection. The vibrated rats weighed more than the control group (1353.0 ± 21.0 vs. 157.0 ± 36.0g, P < 0.048). The plasma Cu and Zn concentrations, vitamin C, uric acid, the activities of antioxidant enzymes, total antioxidant capacity, and malondialdehyde (MDA) levels were similar in the vibration group. No major differences was observed for selected plasma antioxidant parameters . The potential effects of physiological responses of WBV on several physiological systems are without deteriorations concerning plasma antioxidant status.

Effect of increasing levels of bioflavonoids in broiler feed on plasma anti-oxidative potential, lipid metabolites, and fatty acid composition of meat

This study was conducted to investigate the supplemental effects of purified bioflavonoids (genistein and hesperidin), as potential alternatives to plant/herbs or synthetic antioxidants, individually and in combination for fatty acid profile, lipid metabolites, and antioxidant status of broilers. Three hundred sixty 1-d-old broilers were divided into 6 treatment groups: control (basal diet), G5 (5 mg of genistein per kg of feed), and H20 (20 mg hesperidin per kg of feed), whereas the other 3 groups were supplemented with a mixture of genistein and hesperidin (20% genistein + 80% hesperidin) having a dosage of 5 mg•kg(-1) (GH5), 10 mg•kg(-1) (GH10), and 20 mg•kg(-1) (GH20), respectively. Broilers were slaughtered at 42 d, and breast muscle, liver, and blood samples were collected. A dose-dependent increase (P < 0.05) was observed for plasma antioxidant parameters, including total antioxidant capacity, malondialdehyde production, and total superoxide dismutase activity. Cholesterol and triglyceride contents were found to decrease (P < 0.05) in serum and breast muscle. The proportion of total polyunsaturated fatty acids and the ratio of n-6 to n-3 fatty acids and polyunsaturated fatty acids to saturated fatty acids in breast muscles was significantly improved (P < 0.05) by increasing levels of dietary bioflavonoids. The current results implied that dietary bioflavonoids genistein and hesperidin could positively improve the fatty acid and lipid metabolite profile of broiler breast meat in a dose-dependent fashion. Thus, bioflavonoids could be a feasible alternative of antioxidant plants/herbs and synthetic feed additives for the production of healthier chicken meat.

Effects of dietary supplementation of tea saponins (Ilex kudingcha C.J. Tseng) on ruminal fermentation, digestibility and plasma antioxidant parameters in goats

Four goats (25±2.5kg) fitted with the ruminal, duodenal and ileal cannulae were used in a 4×4 Latin square design experiment to determine effects of dietary supplementation of tea saponins (TS) extracted from tea leaves (Ilex kudingcha C.J. Tseng) on ruminal fermentation, digestibility and plasma antioxidant parameters in goats. The goats were fed the same basal diet with levels of TS supplementation of 0 (TS0), 400 (TS400), 600 (TS600) or 800 (TS800) mg TS/kg dry matter (DM). Dietary TS levels did not affect apparent disappearance in the forestomach of DM, N, neutral detergent fiber (NDFom) or acid detergent fiber (ADFom), or apparent digestibility in the small intestine of NDFom and ADFom. Ruminal pH, ammonia N and volatile fatty acid concentrations were not affected by TS addition, as was the plasma concentrations of glutathione peroxidase and malondialdehyde. However triacylglycerol level declined linearly (P<0.01) with increasing levels of TS addition, Results indicate that dietary supplementation of TS did not affect nutrient digestibility, patterns of rumen fermentation, and plasma metabolite concentrations.

Status of lipidsoluble antioxidants and TRAP in patients with Crohn's disease and healthy controls.

To assess the status of lipidsoluble antioxidants (carotenoids, tocopherols, ubiquinone), retinol and their correlation with TRAP (total radical-trapping antioxidant potential) in patients with Crohn's disease. Prospective case-control study. Clinic of Internal Medicine IV/Department of Gastroenterology and Hepatology, University of Vienna Medical School. Plasma antioxidant concentrations were determined in patients with Crohn's disease (n = 24) to evaluate the antioxidant capacity compared to healthy controls (n = 33). Additionally, plasma TRAP (total radical-trapping antioxidant potential) was measured in 13 patients and 22 controls. All investigated carotenoids (alpha-carotene, beta-carotene and cryptoxanthin) were significantly decreased in patients with Crohn's disease (10.2 +/- 9.3, 16.2 +/- 12.4 and 7.8 +/- 5.5 microg/dl) compared to controls (13.3 +/- 5.1, 34.7 +/- 18.8 and 48.5 +/- 38.4 microg/dl respectively), whereas gamma-tocopherol and ubiquinone were significantly elevated in patients (0.14 +/- 0.07 microg/dl and 82.3 +/- 41.5 microg/dl, controls: 0.09 +/- 0.04 microg/dl and 60.8 +/- 30.0 microg/dl, respectively). Retinol and alpha-tocopherol did not significantly differ from controls. The total radical-trapping antioxidant potential (TRAP) was significantly lower in patients (1.11 +/- 0.28 micromol/l) compared to controls (1.34 +/- 0.26 micromol/l). Antioxidants were neither related to duration or severity of disease nor to disease activity. In patients with Crohn's disease several plasma antioxidant parameters are altered and the total radical-trapping antioxidant potential is decreased.