Stroke is a severe that restricts the individual's functions, effects the oral health. The aim of this study was to investigate the relationship between oral health and vitamin D levels in stroke patients in rehabilitation units. Participants with stroke, and age- and sex-matched healthy controls were recruited for the study. Oral Health Impact Scale-14 (OHIP-14), Decayed, Missing, Filled Teeth (DMFT) Index, Community Periodontal Index of Treatment Needs (CPITN), Gingival Index (GI), and Plaque Index (PI) were assesed for all participants. Barthel Index (BI), and Brunnstrom Recovery Scale (BRS), and Modified Ashworth Scale (MAS) assessed for the stroke patients. Vitamin D levels of each participant were recorded. The stroke patients' DMFT index and OHIP-14 values were higher than those of the healthy controls (p<0.05). However, no significant differences were observed in terms of CPTIN, PI, or GI scores (p>0.05). No statistically significant difference was determined in the DMFT index, OHIP-14 scores, Community Periodontal Index of Treatment Needs (CPITN), PI (Plaque Index), and GI (Gingival Index) scores according to vitamin D levels among the stroke patients (p>0.05). Stroke patients' functional scores (BRS, MAS, and BI), vitamin D levels, and oral health status (OHIP-14, DMFT, CPITN, PI, and GI) were subjected to correlation analysis. This revealed significant negative correlation between BRS (arm, hand, and leg), and BI and OHIP-14 scores (p<0.05). Although the DMFT index and health-related quality of life were adversely affected in stroke patients, no relationship was found between vitamin D and oral health status.